Effect of DNA loop anchorage regions (LARs) and microinjection timing on expression of beta-galactosidase gene injected into one-cell rabbit embryos.


:The conditions favoring expression of a reporter gene microinjected into a male pronucleus of naturally ovulated and fertilized rabbit eggs have been studied. Injection of the reporter gene during S phase of the cell-cycle allows the highest level of expression of the gene. Incorporation of DNA loop anchorage regions (LARs) into constructs upstream and/or downstream of the reporter gene significantly increased the efficiency of expression. In all cases the expression of the microinjected gene started after a period of transcriptional quiescence, i.e., together with the expression of the host genome. Correct targeting of microinjected constructs within the nuclei via interaction of LAR elements with receptor sites on the nucleoskeleton may facilitate expression of injected DNA constructs as well as their integration into host cell DNA.


J Cell Biochem


Goldman IL,Razin SV,Kadulin SG,Vassetzky YS




Has Abstract


2004-08-15 00:00:00












  • Neonatal treatment with fluoxetine improves mitochondrial respiration and reduces oxidative stress in liver of adult rats.

    abstract::Recent studies have shown that exposure to fluoxetine treatment induces excessive production of ROS, and alters the antioxidant defense system in various tissues and cell types, mainly the liver. When fluoxetine is administered intraperitoneally, the drug rapidly reaches high concentrations in the liver, has potential...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Simões-Alves AC,Silva-Filho RC,Braz GRF,Silva SCA,da Silva AI,Lagranha CJ,Fernandes MP

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    abstract::Embryo development block seriously limits the success of in vitro embryo production and assisted reproductive technology. Although numerous researchers have explored this problem, it remains to be solved. In this study, we found that melatonin supplementation at 10-8 and 10-9 M in M16 significantly reduced two-cell bl...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Shi F,Li H,Wang E,Chen ZJ,Zhang C

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  • Impact of HMG-CoA reductase inhibition on oxidant-induced injury in human retinal pigment epithelium cells.

    abstract::In addition to cholesterol-lowering effect, HMG-CoA reductase inhibition by statins has been shown to have protective effect in many cells type. The loss of vision in retinal degeneration disease associates with oxidative stress and apoptosis in retinal pigment epithelium (RPE) cell. This study was designed to examine...

    journal_title:Journal of cellular biochemistry

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    authors: Qian J,Keyes KT,Long B,Chen G,Ye Y

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  • Pyrazinamide drug resistance in RpsA mutant (∆438A) of Mycobacterium tuberculosis: Dynamics of essential motions and free-energy landscape analysis.

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    authors: Singh A,Somvanshi P,Grover A

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  • Biochemical regulation and structural analysis of copper-transporting ATPase in a human hepatoma cell line for Wilson disease.

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    pub_type: 杂志文章


    authors: Agnihotry S,Dhusia K,Srivastav AK,Upadhyay J,Verma V,Shukla PK,Ramteke PW,Gautam B

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    journal_title:Journal of cellular biochemistry

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    authors: Bortell R,Owen TA,Ignotz R,Stein GS,Stein JL

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    authors: Maqbool F,Bahadar H,Hassani S,Niaz K,Baeeri M,Rahimifard M,Ghasemi-Niri SF,Abdollahi M

    更新日期:2019-09-01 00:00:00

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Westendorf JJ

    更新日期:2006-05-01 00:00:00

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    authors: Underwood JM,Becker KA,Stein GS,Nickerson JA

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    pub_type: 杂志文章


    authors: Park YS,Liu Z,Vasamsetti BM,Cho NJ

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


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  • Nitric oxide from both exogenous and endogenous sources activates mitochondria-dependent events and induces insults to human chondrocytes.

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    authors: Wu GJ,Chen TG,Chang HC,Chiu WT,Chang CC,Chen RM

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    authors: Civitelli R,Ziambaras K,Warlow PM,Lecanda F,Nelson T,Harley J,Atal N,Beyer EC,Steinberg TH

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  • Study on the relationship between insulin growth factor 1 and liver fibrosis in patients with chronic hepatitis C with type 2 diabetes mellitus.

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    authors: Cao LH,Lu FM,Lu XJ,Zhu LY

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  • 7-Dehydrocholesterol (7-DHC), But Not Cholesterol, Causes Suppression of Canonical TGF-β Signaling and Is Likely Involved in the Development of Atherosclerotic Cardiovascular Disease (ASCVD).

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    authors: Altenhofen D,da Luz G,Frederico MJ,Venzke D,Brich M,Vigil S,Fröde TS,Linares CE,Pizzolatti MG,Silva FR

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  • Saitohin, which is nested within the tau gene, interacts with tau and Abl and its human-specific allele influences Abl phosphorylation.

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cheng TJ,Lai YK

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    authors: Parker MG,Webb P,Needham M,White R,Ham J

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ahmad A,Banerjee S,Wang Z,Kong D,Sarkar FH

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  • Post-transcriptional gene regulation by gamma herpesviruses.

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Swaminathan S

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  • KRAS mutation and abnormal expression of Cripto-1 as two potential candidate biomarkers for detection of colorectal cancer development.

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Igder S,Mohammadiasl J,Azadpour S,Mansouri E,Ashktorab H,Mokarram P

    更新日期:2020-04-01 00:00:00

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Li Y,Zhao W,Shi R,Jia J,Li X,Cheng J

    更新日期:2018-11-01 00:00:00

  • TNFalpha mediates sepsis-induced impairment of basal and leucine-stimulated signaling via S6K1 and eIF4E in cardiac muscle.

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lang CH,Pruznak AM,Frost RA

    更新日期:2005-02-01 00:00:00

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Benzakour O,Kanthou C,Lupu F,Dennehy U,Goodwin C,Scully MF,Kakkar VV,Cooper DN

    更新日期:1995-12-01 00:00:00

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    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Katakawa Y,Funaba M,Murakami M

    更新日期:2016-08-01 00:00:00