GABA uptake into isolated retinal Müller glial cells of the guinea-pig detected electrophysiologically.


:In order to investigate GABA uptake into Müller glial cells electrophysiologically, we used the whole-cell voltage-clamp technique. Since Müller cells were insensitive to application of muscimol and baclofen, the expression of GABAA and GABAB receptors can be excluded. Therefore, the observed GABA current must be due to an electrogenic GABA transporter. This transporter is driven by the transmembrane Na+ gradient, as replacement of the extracellular Na+ by choline inhibits the GABA current. Currents evoked by cis-4-aminocrotonic acid, a GABAC specific agonist, are also inhibited by replacement of extracellular Na+, indicating that this compound is a substrate for the uptake. The competitive GABA uptake blockers beta-alanine and nipecotic acid are substrates of the transporter as well, and produce 42% and 65% of the currents elicited by the same GABA concentration, respectively. Affinity of the transporter for GABA is high, the Km value being 5 microM at -80 mV.






Biedermann B,Eberhardt W,Reichelt W




Has Abstract


1994-01-12 00:00:00












  • Polyamines modulate the function of transfected glutamate receptor mGluR1a.

    abstract::Metabotropic glutamate receptor mGluR1a was expressed in human embryonic kidney 293 cells. 1S,3R-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) stimulated dose-dependently, phosphoinositide (PI) hydrolysis in transfected, but not in non-transfected cells. The polyamine spermine did not affect PI hydrolysis in th...


    pub_type: 杂志文章


    authors: Manev RM,Gabellini N,Manev H

    更新日期:1993-06-01 00:00:00

  • Mechanisms of nicotine mediated communication between NGF-differentiated PC12 and HEL cells.

    abstract::HEL 92.1.7 cells were immobilized among nerve growth factor (NGF)-differentiated PC12 cells. Nicotine caused an immediate Ca2+ mobilisation in the PC12 cells followed by a delayed secondary Ca2+ response in the HEL 92.1.7 cells. The Ca2+ elevation in response to nicotine in PC12 cells was abolished by Na+ removal. The...


    pub_type: 杂志文章


    authors: Krjukova J,Akerman KE

    更新日期:2002-07-02 00:00:00

  • Centrally injected kisspeptin reduces food intake by increasing meal intervals in mice.

    abstract::Kisspeptin is distributed not only in brain areas for regulating reproduction but also in nuclei involved in feeding control. Whether kisspeptin alters food intake is unknown in mice. We examined how kisspeptin-10 influences feeding after intracerebroventricular injection in mice using automated monitoring. Kisspeptin...


    pub_type: 杂志文章


    authors: Stengel A,Wang L,Goebel-Stengel M,Taché Y

    更新日期:2011-03-30 00:00:00

  • Developmental expression and localization of KCNJ10 K+ channels in the guinea pig inner ear.

    abstract::The inward rectifier Kir4.1, composed of KCNJ10 K channel subunits, plays an essential role in inner ear K homeostasis. We have investigated the developmental expression and localization of KCNJ10 (Kir4.1) in the guinea pig inner ear using semi-quantitative reverse transcription polymerase chain reaction and immunohis...


    pub_type: 杂志文章


    authors: Jin Z,Wei D,Järlebark L

    更新日期:2006-04-03 00:00:00

  • Interindividual and interspecies variations of the extrastriate visual cortex.

    abstract::Functional homology between human and macaque visual cortices has provided an important cue to functional subdivisions of the human visual cortex, but it is unclear beyond V1. We estimated the sizes and the visual field eccentricity functions of the extrastriate visual areas of human brains using MRI and fMRI measurem...


    pub_type: 杂志文章


    authors: Ejima Y,Takahashi S,Yamamoto H,Fukunaga M,Tanaka C,Ebisu T,Umeda M

    更新日期:2003-08-26 00:00:00

  • An IP3-assisted form of Ca2+-induced Ca2+ release in neocortical neurons.

    abstract::Calcium increases induced by single action potentials in rat visual cortex layer II/III pyramidal neurons were shown to be augmented by muscarinic acetylcholine receptor (mAchR) stimulation. This augmentation was drastically reduced by intracellular injection of heparin but not ruthenium red, therefore involving inosi...


    pub_type: 杂志文章


    authors: Yamamoto K,Hashimoto K,Isomura Y,Shimohama S,Kato N

    更新日期:2000-02-28 00:00:00

  • Structural and functional connectivity correlates with motor impairment in chronic supratentorial stroke: a multimodal magnetic resonance imaging study.

    abstract::The aim of this study was to identify differences in structural and functional brain connectivity between poststroke mild and severe motor impairment. Twenty-four chronic stroke patients who underwent resting-state functional MRI and diffusion tensor image were retrospectively included. All patients were classified in...


    pub_type: 杂志文章


    authors: Lee JH,Kyeong S,Kang H,Kim DH

    更新日期:2019-05-01 00:00:00

  • Error-related negativities elicited by monetary loss and cues that predict loss.

    abstract::Event-related potential studies have reported error-related negativity following both error commission and feedback indicating errors or monetary loss. The present study examined whether error-related negativities could be elicited by a predictive cue presented prior to both the decision and subsequent feedback in a g...


    pub_type: 杂志文章


    authors: Dunning JP,Hajcak G

    更新日期:2007-11-19 00:00:00

  • Complete mutism after midbrain periaqueductal gray lesion.

    abstract::Several neurophysiological studies have highlighted the role of the midbrain periaqueductal gray matter (PAG) in the initiation of vocalization in various animal species, from frogs to primates. With regard to humans, only two cases of complete mutism following a lesion to the PAG have been reported so far. This artic...


    pub_type: 杂志文章


    authors: Esposito A,Demeurisse G,Alberti B,Fabbro F

    更新日期:1999-03-17 00:00:00

  • Developmental changes in optokinetic mechanisms in the absence of unilateral cortical control.

    abstract::Animal models suggest that the asymmetry of monocular optokinetic nystagmus (OKN) in young infants can be explained by a direct pathway from retina to the midbrain nucleus of the optic tract. However, earlier studies with hemispherectomized infants showed no evidence for OKN responses towards the damaged cortex that c...


    pub_type: 杂志文章


    authors: Morrone MC,Atkinson J,Cioni G,Braddick OJ,Fiorentini A

    更新日期:1999-09-09 00:00:00

  • Judging semantic and episodic incongruity: a magnetoencephalographic study.

    abstract::Evoked magnetic fields over the entire head were measured during a semantic judgment task, in which conditions of the pre-test learning session were manipulated. Subjects learnt related and unrelated category-noun pairs, and were then asked to judge the relationship between category and noun. Unrelated pairs evoked ac...


    pub_type: 杂志文章


    authors: Suzuki K,Okuda J,Otsuka Y,Sugawara A,Hatanaka K,Nakasato N,Kanno A,Yoshimoto T,Fujii T,Yamadori A

    更新日期:2001-02-12 00:00:00

  • Older adults exhibit greater brain activity than young adults in a selective inhibition task by bipedal and bimanual responses: an fNIRS study.

    abstract::Inhibitory deficits are one of the predominant causes of cognitive aging. This study examined age-related changes in response inhibition. In this study, young and older participants performed a bimanual/bipedal response inhibition task while we measured their brain activity via functional near-infrared spectroscopy. P...


    pub_type: 杂志文章


    authors: Kawai N,Nakata R,Kubo-Kawai N

    更新日期:2020-10-07 00:00:00

  • Transneuronal viral labelling of rat heart left ventricle controlling pathways.

    abstract::Retrograde transneuronal viral labelling and immunocytochemical methods were used for revealing neuronal networks controlling the left ventricle myocardium of the rat heart. After injections of 1 microliter pseudorabies virus solution (3 x 10(6) PFU ml-1) into the left ventricle, infected orthosympathetic preganglioni...


    pub_type: 杂志文章


    authors: Ter Horst GJ,Van den Brink A,Homminga SA,Hautvast RW,Rakhorst G,Mettenleiter TC,De Jongste MJ,Lie KI,Korf J

    更新日期:1993-09-30 00:00:00

  • Role of exosomes induced by remote ischemic preconditioning in neuroprotection against cerebral ischemia.

    abstract::Remote ischemic preconditioning (RIPC) is an effective regimen for neuroprotection in ischemic stroke. Exosomes are extracellular vesicles released into the blood, where they can transfer signals throughout the body. Several studies have demonstrated that RIPC leads to many changes in circulating exosomes. However, th...


    pub_type: 杂志文章


    authors: Li Y,Ren C,Li H,Jiang F,Wang L,Xia C,Ji X

    更新日期:2019-08-14 00:00:00

  • α1-Adrenoceptors in the hippocampal dentate gyrus involved in learning-dependent long-term potentiation during active-avoidance learning in rats.

    abstract::The hippocampus is the key structure for learning and memory in mammals and long-term potentiation (LTP) is an important cellular mechanism responsible for learning and memory. The influences of norepinephrine (NE) on the modulation of learning and memory, as well as LTP, through β-adrenoceptors are well documented, w...


    pub_type: 杂志文章


    authors: Lv J,Zhan SY,Li GX,Wang D,Li YS,Jin QH

    更新日期:2016-11-09 00:00:00

  • Long-term morphine treatment increases Ku protein DNA end-binding activity.

    abstract::Human neuroblastoma SH-SY5Y and small-cell lung carcinoma U1690 cells of neuroendocrine origin were exposed to morphine for 1 h, 3 h or 5 days. These treatments did not alter activities of AP-1, NF-kappa B and YY1 transcription factors in SH-SY5Y cells or NF-kappa B and YY1 in U1690 cells. Five-day morphine treatment,...


    pub_type: 杂志文章


    authors: Bakalkin G,Yakovleva T,Melzig M,Terenius L

    更新日期:1997-07-07 00:00:00

  • Age-dependent changes in distribution of P3a/P3b amplitude and thickness of the cerebral cortex.

    abstract::With increasing age, the electrophysiological event-related potentials P3a/P3b tend to get a more frontal maximum. The cognitive significance of this so-called frontal shift is not known, but hypotheses have focused on changes in the integrity of the frontal lobes. The aim of the present study was to test how the thic...


    pub_type: 杂志文章


    authors: Fjell AM,Walhovd KB,Reinvang I

    更新日期:2005-09-08 00:00:00

  • Intracellular calcium signals in the surround of rat visual cortex lesions.

    abstract::Focal lesions of the visual cortex induce deafferentiation, excitotoxic cell death as well as functional reorganization in the surrounding tissue. The intracellular second messenger calcium is involved in a wide range of cellular responses including excitotoxicity and functional reorganization following cortical injur...


    pub_type: 杂志文章


    authors: Barmashenko G,Eysel UT,Mittmann T

    更新日期:2001-10-08 00:00:00

  • Decreased calmodulin-NR1 co-assembly as a mechanism for focal epilepsy in cortical dysplasia.

    abstract::The NMDA receptor is one of the ionotropic glutamate receptors essential for excitatory neurotransmission. The NMDAR1 subunit is inactivated by direct interaction with calmodulin. The protein levels of calmodulin, NMDAR1 and their complex were quantified in tissue resected from epileptogenic and non-epileptogenic cort...


    pub_type: 临床试验,杂志文章


    authors: Mikuni N,Nishiyama K,Babb TL,Ying Z,Najm I,Okamoto T,Lüders HO,Wylie C

    更新日期:1999-05-14 00:00:00

  • Paraventricular nucleus injections of naloxone methiodide inhibit NPY's effects on energy substrate utilization.

    abstract::Microinjection of neuropeptide Y (NPY) into the paraventricular nucleus (PVN) of the hypothalamus stimulates eating and increases respiratory quotient. In contrast, administration of opioid receptor antagonists reduces food intake and suppresses NPY-induced feeding. The present study examined whether naloxone methiodi...


    pub_type: 杂志文章


    authors: Currie PJ,Coscina DV,Moretti J,Avellino MD

    更新日期:2000-03-20 00:00:00

  • Magno- and parvocellular pathways are segregated in the human optic tract.

    abstract::There is abundant psychophysical evidence in humans suggesting the existence of parallel pathways subserving different aspects of vision. However, there is little direct proof of the neural structures underlying the two pathways. We present direct anatomical evidence that in the normal human optic tract fibres are seg...


    pub_type: 杂志文章


    authors: Tassinari G,Campara D,Balercia G,Chilosi M,Martignoni G,Marzi CA

    更新日期:1994-07-21 00:00:00

  • Electrophysiological correlates of visual consciousness and selective attention.

    abstract::It is not clear whether attention is necessary or not for consciousness. We studied the relationship between attention and consciousness by tracking their electrophysiological correlates. The participants attended to visual targets, ignored nontargets in the prespecified visual field and ignored all stimuli in the opp...


    pub_type: 杂志文章


    authors: Koivisto M,Revonsuo A

    更新日期:2007-05-28 00:00:00

  • Rotenone inhibits delayed rectifier K+ current via a protein kinase A-dependent mechanism.

    abstract::Voltage-gated K+ channels (K(V)) regulate cell electrical properties, proliferation, migration, and death. Rotenone is a mitochondrial inhibitor, influencing activity of many channels that potentially participate in cell death processes, but its effect on K(V) channel in neurons remains unclear. This study used whole-...


    pub_type: 杂志文章


    authors: Gao XF,Wang W,He C

    更新日期:2008-09-17 00:00:00

  • Grating and plaid chrominance motion influences the suppressed ocular following response.

    abstract::Involuntary eye movements to foveal stimulation were measured in a monkey while it performed a fixation task. Second-order plaid motion generated higher velocities of eye movements than did first-order gratings, yet the latency of the early following response was no different for grating or plaid motion. Nevertheless,...


    pub_type: 杂志文章


    authors: Guo K,Benson PJ

    更新日期:1999-02-05 00:00:00

  • Correlations, feature-binding and population coding in primary visual cortex.

    abstract::To test the hypothesis that correlated neuronal activity serves as the neuronal code for visual feature binding, we applied information theory techniques to multiunit activity recorded from pairs of V1 recording sites in anaesthetised cats while presenting either single or separate bar stimuli. We quantified the roles...


    pub_type: 杂志文章


    authors: Golledge HD,Panzeri S,Zheng F,Pola G,Scannell JW,Giannikopoulos DV,Mason RJ,Tovée MJ,Young MP

    更新日期:2003-05-23 00:00:00

  • Genetic and phenotypic characteristics of SCN1A-related epilepsy in Chinese children.

    abstract::SCN1A gene mutations are associated with epilepsy and neurodevelopmental disorders. This study aimed to explore the genotype and phenotype spectrum of SCN1A gene related epilepsy. Epileptic patients who were treated in the Children's Hospital of Chongqing Medical University from January 2015 to July 2018 and identifie...


    pub_type: 杂志文章


    authors: Fang ZX,Hong SQ,Li TS,Wang J,Xie LL,Han W,Jiang L

    更新日期:2019-06-12 00:00:00

  • Neuroprotective effects of 17β-estradiol associate with KATP in rat brain.

    abstract::Previous studies have indicated that estrogen protects the brain from ischemic damage and regulates K(ATP) channel activity; the present study was designed to address the involvement of K(ATP) channels in the neuroprotective effects of estrogen in focal cerebral ischemia: in experiment 1, K(ATP) mRNA and protein in th...


    pub_type: 杂志文章


    authors: Zhang D,Xia H,Xu L,Zhang C,Yao W,Wang Y,Ren J,Wu J,Tian Y,Liu W,Wang X

    更新日期:2012-11-14 00:00:00

  • Effects of aging on P300 between late young-age and early middle-age adulthood: an electroencephalogram event-related potential study.

    abstract::The aim of this study was to identify age-related changes of P300 peak amplitude and P300 latency between closely separated nonsenile age groups (late young-aged adults and early middle-aged adults) and to investigate whether or not P300 has the potential to be used as a measure of cognitive aging even among nonsenile...


    pub_type: 杂志文章


    authors: Bourisly AK

    更新日期:2016-09-28 00:00:00

  • N-methyl D-aspartate induced mechanical allodynia is blocked by nitric oxide synthase and cyclooxygenase-2 inhibitors.

    abstract::The role of spinal NMDA receptors in mechanical nociceptive processing was assessed in sheep. Intrathecal NMDA (2 nmol-1 micromol) produced a significant reduction in mechanical withdrawal thresholds. This effect was attenuated by pretreatment with the NMDA receptor antagonist MK801 (100 nmol), the cyclooxygenase-2 (C...


    pub_type: 杂志文章


    authors: Dolan S,Nolan AM

    更新日期:1999-02-25 00:00:00

  • GPR39 receptor expression in the mouse brain.

    abstract::GPR39, an orphan G protein-coupled receptor, has been recently identified as the receptor for the bioactive peptide obestatin. Obestatin is secreted from the stomach and acts as an anti-appetite hormone. This activity is induced whether obestatin is administered intraperitoneally or intracerebroventricularly. GPR39 is...


    pub_type: 杂志文章


    authors: Jackson VR,Nothacker HP,Civelli O

    更新日期:2006-05-29 00:00:00