Evaluation of mixed chimerism by two-step polymerase chain reaction amplification of hypervariable region MCT118 after allogeneic bone marrow transplantation.


:We assessed the origin of peripheral blood cells and bone marrow cells of 92 samples obtained from 19 patients after allo BMT by two-step polymerase chain reaction (PCR) amplification of MCT118, one of the variable number of tandem repeat regions (VNTR) which has a different length in 19 of 32 sibling pairs examined, that can detect the DNA pattern of a minor cell population of only 1% without using radioisotopes. Mixed chimerism (MC) was detected in the hematopoietic cells of four patients. Two patients who showed progressive MC developed relapse of leukemia 3 months and 4 months after the detection of MC, and two patients died with bone marrow hypoplasia 61 days and 7 months after BMT. These data suggest the clinical importance of analyzing the correlation of MC and clinical complications after BMT by this method, which can be used to monitor MC in about two thirds of allo BMT patients with an adequate sensitivity.


Ann Hematol


Annals of hematology


Tanaka J,Kasai M,Imamura M,Higa T,Kobayashi S,Hashino S,Sakurada K,Miyazaki T




Has Abstract


1994-04-01 00:00:00












  • Thrombotic thrombocytopenic purpura without any evidence of thrombotic lesions at autopsy.

    abstract::Diagnosis of thrombotic thrombocytopenic purpura (TTP) is usually based upon the clinical features, and does not always involve histopathological evidence. We recently had experience with a patient who developed the five signs characteristic for TTP. He had been treated for liver cirrhosis associated with chronic hepa...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Hori A,Kami M,Hamaki T,Yamamoto T,Mori M,Miyakoshi S,Takeuchi K

    更新日期:2001-08-01 00:00:00

  • Trends in incidence and medical resource utilisation in patients with chronic lymphocytic leukaemia: insights from the UK Clinical Practice Research Datalink (CPRD).

    abstract::Chronic lymphocytic leukaemia (CLL) is the most common leukaemia in European adults. We aimed to evaluate time trends in CLL incidence and medical resource utilisation of CLL patients in the UK. We conducted a retrospective, observational cohort analysis using the UK Clinical Practice Research Datalink (CPRD) comprisi...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Pfeil AM,Imfeld P,Pettengell R,Jick SS,Szucs TD,Meier CR,Schwenkglenks M

    更新日期:2015-03-01 00:00:00

  • The predictive value of selected serum microRNAs for acute GVHD by TaqMan MicroRNA arrays.

    abstract::Currently, the diagnosis of acute graft-versus-host disease (aGVHD) is mainly based on clinical symptoms and biopsy results. This study was designed to further explore new no noninvasive biomarkers for aGVHD prediction/diagnosis. We profiled miRNAs in serum pools from patients with aGVHD (grades II-IV) (n = 9) and non...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Zhang C,Bai N,Huang W,Zhang P,Luo Y,Men S,Wen T,Tong H,Wang S,Tian YP

    更新日期:2016-10-01 00:00:00

  • Acute porphyrias: a German monocentric study of the biochemical, molecular genetic, and clinical data of 62 families.

    abstract::In Germany, analyses of clinical and laboratory features of patients with acute porphyrias are only available for hereditary coproporphyria (HCP) but not with other acute porphyrias, acute intermittent porphyria (AIP) and variegate porphyria (VP). The aim of the study was to analyze a large cohort of patients with par...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Bronisch O,Stauch T,Haverkamp T,Beykirch MK,Petrides PE

    更新日期:2019-12-01 00:00:00

  • Non-invasive urinary biomarkers of renal function in sickle cell disease: an overview.

    abstract::Sickle cell disease (SCD) is a hereditary condition characterized by homozygosis of the hemoglobin S (HbS) gene. Marked morbimortality is observed due to chronic hemolysis, endothelial injury, and episodes of vaso-occlusion, which leads to multi-organ damage. Renal impairment is common and may have different presentat...

    journal_title:Annals of hematology

    pub_type: 杂志文章,评审


    authors: Laurentino MR,Parente Filho SLA,Parente LLC,da Silva Júnior GB,Daher EF,Lemes RPG

    更新日期:2019-12-01 00:00:00

  • Predictors of plasma cell disorders among African American patients: a community practice perspective.

    abstract::African Americans have two- to three-fold higher incidence of multiple myeloma and MGUS compared to other ethnic groups in the USA. Some physicians often perform diagnostic evaluations for plasma cell disorders (PCD) in African American patients on the basis of hematological abnormalities (thrombocytopenia, leucopenia...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Tun NM,Joseph G,Soe AM,Ford JG

    更新日期:2014-06-01 00:00:00

  • Immunoglobulin serum levels in very low birth weight infants treated with different intravenous preparations.

    abstract::Parenteral human immunoglobulin (IVIG) administration is widely used in low birth weight (LBW) infants for prevention and therapy of neonatal infection. In previous studies, IVIG preparations containing IgG and low IgM concentrations were commonly used. In this study we compare immunoglobulin serum levels in two group...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Amato M,Markus D,Hüppi P,Imbach P

    更新日期:1991-10-01 00:00:00

  • Cytogenetic profiles of 2806 patients with acute myeloid leukemia-a retrospective multicenter nationwide study.

    abstract::The cytogenetic and molecular data is recognized as the most valuable prognostic factor in acute myeloid leukemia (AML). Our aim was to systemically analyze the cytogenetics of Korean AML patients and to compare the cytogenetic profiles of various races to identify possible geographic heterogeneity. We retrospectively...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Byun JM,Kim YJ,Yoon HJ,Kim SY,Kim HJ,Yoon J,Min YH,Cheong JW,Park J,Lee JH,Hong DS,Park SK,Kim HJ,Ahn JS,Shin HJ,Chung JS,Lee WS,Lee SM,Park Y,Kim BS,Lee JH,Lee KH,Jung CW,Jang JH,Min WS,Park TS,AML\/M

    更新日期:2016-08-01 00:00:00

  • Evaluation of neutrophil-lymphocyte ratio in patients with early-stage mycosis fungoides.

    abstract::Neutrophil-lymphocyte ratio (NLR), an indicator of inflammation, has been lately demonstrated as a prognostic factor and an indicator of disease activity in various diseases. However, the effects of NLR have not been investigated in mycosis fungoides (MF) patients yet. The aim of this study is to investigate the relat...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Eren R,Nizam N,Doğu MH,Mercan S,Erdemir AV,Suyanı E

    更新日期:2016-10-01 00:00:00

  • Evaluation of a new protein-C concentrate and comparison of protein-C assays in a child with congenital protein-C deficiency.

    abstract::We investigated a new protein-C (PC) concentrate in a child with a type-II homozygous deficiency, concerning tolerance and safety. By means of various functional and antigen assays the in vivo recovery and the half-life were determined. In order to compare the results we reduced the measured values to the average half...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Auberger K

    更新日期:1992-03-01 00:00:00

  • The effect of pre-eclampsia on complete blood count, platelet count and mean platelet volume.

    abstract::Pre-eclampsia is a condition observed during pregnancy and threatens the life of both mother and foetus. There are studies, which suggest platelets play a major role in the pathogenesis of pre-eclampsia. The aim of this study is to compare the complete blood count (CBC) parameters, especially platelet count and mean p...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Ceyhan T,Beyan C,Başer I,Kaptan K,Güngör S,Ifran A

    更新日期:2006-05-01 00:00:00

  • Good adherence to imatinib therapy among patients with chronic myeloid leukemia--a single-center observational study.

    abstract::Previous studies have suggested that adherence to imatinib therapy can be an obstacle among patients with chronic myeloid leukemia (CML). We studied adherence to imatinib therapy among CML patients treated at the Sahlgrenska University Hospital. We identified all CML patients that were alive at the 1st of January 2010...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Jönsson S,Olsson B,Söderberg J,Wadenvik H

    更新日期:2012-05-01 00:00:00

  • Presence of a high-grade component in gastric mucosa-associated lymphoid tissue (MALT) lymphoma is not associated with an adverse prognosis.

    abstract::Gastric mucosa-associated lymphoid tissue (MALT) lymphoma and diffuse large B cell lymphoma (DLBCL) show a spectrum of disease characterized by varying proportions of low-grade and high-grade components. While the natural history and optimum treatment for low-grade gastric MALT lymphoma and DLBCL is well established, ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Ang MK,Hee SW,Quek R,Yap SP,Loong S,Tan L,Tao M,Lim ST

    更新日期:2009-05-01 00:00:00

  • A phase 1 clinical trial of flavopiridol consolidation in chronic lymphocytic leukemia patients following chemoimmunotherapy.

    abstract::Patients with chronic lymphocytic leukemia (CLL) who receive chemoimmunotherapy and do not achieve complete remission experience significantly shortened progression-free interval (PFS). Additionally, the majority of patients treated for relapsed disease demonstrate evidence of measurable disease. Eradication of minima...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Awan FT,Jones JA,Maddocks K,Poi M,Grever MR,Johnson A,Byrd JC,Andritsos LA

    更新日期:2016-06-01 00:00:00

  • Predictors of clinical responses to hypomethylating agents in acute myeloid leukemia or myelodysplastic syndromes.

    abstract::Azacitidine and decitabine, two hypomethylating agents, are known to be effective in the treatment of high-risk myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) patients who cannot endure intensive cytotoxic chemotherapy or are not eligible for transplantation. However, the treatment response rate is l...

    journal_title:Annals of hematology

    pub_type: 杂志文章,评审


    authors: Wang H,Li Y,Lv N,Li Y,Wang L,Yu L

    更新日期:2018-11-01 00:00:00

  • Regulation of FLT3 and its ligand in normal hematopoietic progenitor cells.

    abstract::FLT3 and its ligand (FL) are one of the regulators of normal hematopoiesis. Ligand-independent activation of FLT3 occurs in about 30% of acute myeloid leukemia cases and is one goal for selectively targeted therapies. However, the function of FLT3/FL in the regulation of non-malignant immature hematopoietic cells is p...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Weisel KC,Yildirim S,Schweikle E,Kanz L,Möhle R

    更新日期:2009-03-01 00:00:00

  • Thrombotic thrombocytopenic purpura in a patient with sickle cell crisis.

    abstract::The combination of sickle cell disease crisis and thrombotic thrombocytopenic purpura has been described only a few times. Here we present the case of a patient with a hemolytic crisis due to sickle cell disease complicated by thrombotic thrombocytopenic purpura. We also review the cases previously reported and compar...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Bolaños-Meade J,Keung YK,López-Arvizu C,Florendo R,Cobos E

    更新日期:1999-12-01 00:00:00

  • Neutropenic enterocolitis in acute leukemia: diagnostic and therapeutic dilemma.

    abstract::The main purpose of this report is to focus on the importance of an accurate etiologic diagnosis of gastrointestinal complications during chemotherapy for acute myeloid leukemia, taking into account that a syndrome characterized by bowel wall thickening associated with diarrhea and abdominal pain may have etiologies d...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Capria S,Vitolo D,Cartoni C,Dessanti L,Micozzi A,Mandelli F,Meloni G

    更新日期:2004-03-01 00:00:00

  • Treatment of primary defective iron-reutilization syndrome: revisited.

    abstract::We encountered two patients who presented with hypochromic-microcytic anemia and were refractory to iron therapy. The symptoms were suggestive of anemia of chronic disease (ACD); however, there was no evidence of any such disease, either inflammatory or malignant. These patients were reminiscent of patients originally...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Besa EC,Kim PW,Haurani FI

    更新日期:2000-08-01 00:00:00

  • Phase IV study evaluating efficacy of escalated dose of imatinib in chronic myeloid leukemia patients showing suboptimal response to standard dose imatinib.

    abstract::The aim of this phase IV study was to (1) to define efficacy of escalating dose imatinib in chronic myeloid leukemia (CML) patients showing suboptimal response to standard dose imatinib and (2) to find markers that predict the response to escalating doses of imatinib. CML patients in chronic phase (CP) who failed to a...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Koh Y,Kim I,Yoon SS,Kim BK,Kim DY,Lee JH,Lee KH,Park E,Kim HJ,Sohn SK,Joo YD,Kim SJ,Chung J,Shin HJ,Kim SH,Kim CS,Song HS,Kim MK,Hyun MS,Ahn JS,Jung CW,Park S,Korean Society of Hematology CML working party.

    更新日期:2010-07-01 00:00:00

  • Malignant infantile osteopetrosis initially presenting with neonatal hypocalcemia: case report.

    abstract::Autosomal recessive "malignant" osteopetrosis is a rare congenital disorder relating to bone resorption abnormalities. It is believed to arise due to the failure of osteoclasts to resorb immature bone. This leads to abnormal bone marrow cavity formation and, clinically, to the signs and symptoms of bone marrow failure...

    journal_title:Annals of hematology

    pub_type: 杂志文章,评审


    authors: Chen CJ,Lee MY,Hsu ML,Lien SH,Cheng SN

    更新日期:2003-01-01 00:00:00

  • High leukemia-free survival after TBI-based conditioning and mycophenolate mofetil-containing immunosuppression in patients allografted for chronic myelomonocytic leukemia: a single-center experience.

    abstract::This retrospective single-center analysis studied the impact of the conditioning and the graft-versus-host disease (GVHD) prophylaxis on outcome in unselected patients allografted for chronic myelomonocytic leukemia (CMML) and acute myeloid leukemia (AML) secondary to documented prior CMML. A total of 44 patients (med...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Radujkovic A,Hegenbart U,Müller-Tidow C,Herfarth K,Dreger P,Luft T

    更新日期:2020-04-01 00:00:00

  • Transient spontaneous regression of aggressive non-Hodgkin's lymphoma confined to the adrenal glands.

    abstract::A 72-year-old-man with night sweats and a low-grade fever was found to have bilateral adrenal enlargement associated with incipient adrenal insufficiency. Without any intervention, these adrenal lesions regressed spontaneously, accompanied by disappearance of clinical symptoms. Seven months later, however, the lesions...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Fujiwara T,Kawamura M,Sasaki A,Asahi H,Sasou S,Itoh S,Hiramori K

    更新日期:2001-09-01 00:00:00

  • Failure of repeated courses of high-dose intravenous immunoglobulin to induce stable remission in patients with chronic idiopathic thrombocytopenic purpura.

    abstract::Repeated courses of HD IVIg are reported to induce stable remission in a significant proportion of adults with chronic refractory ITP. We have treated 14 such patients obtaining a remission rate quite comparable to the 5-10% of spontaneous remission. ...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Schiavotto C,Ruggeri M,Rodeghiero F

    更新日期:1995-02-01 00:00:00

  • Acute alveolitis induced by hydroxyurea in a patient with myeloproliferative syndrome.

    abstract::Hydroxyurea is increasingly being used to control myeloproliferative disorders, in part because of its relative lack of side effects. We present a case of life-threatening alveolitis in a patient treated with hydroxyurea for myeloproliferative syndrome. Absence of exposure to other drugs and the clinical course sugges...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Hennemann B,Bross KJ,Reichle A,Andreesen R

    更新日期:1993-09-01 00:00:00

  • Thymoma-associated pancytopenia: effectiveness of cyclosporine A.

    abstract::Aplastic anemia is a rare complication of thymoma and is properly documented in only few cases. Here, we report the case of a previously healthy, 65-year-old patient who was found simultaneously to have a spindle-cell thymoma and severe hypoplastic anemia with a mild infiltration of the bone marrow by CD4+ and CD8+ T ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Liozon E,Touati M,Allegraud A,Gachard N,Loustaud V,Vidal E,Bordessoule D

    更新日期:1998-10-01 00:00:00

  • Risk for cytomegalovirus infection following reduced intensity allogeneic stem cell transplantation.

    abstract::Preliminary data suggest a faster immune recovery following non-myeloablative stem cell transplantation because of the persistence of recipient T cells, but the real impact on post-transplant infectious complications remains unknown. We retrospectively analysed the incidence of cytomegalovirus (CMV) infection in twent...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Nachbaur D,Larcher C,Kircher B,Eibl G,Nussbaumer W,Gunsilius E,Haun M,Grünewald K,Gastl G

    更新日期:2003-10-01 00:00:00

  • Modified BEAM with triple autologous stem cell transplantation for patients with relapsed aggressive non-Hodgkin lymphoma.

    abstract::Treatment of relapse and primary progression in aggressive lymphoma remains unsatisfactory; outcome is still poor. Better treatment strategies are much needed for this patient population. The R1 study is a prospective multi-center phase I/II study evaluating a dose finding approach with a triple transplant regimen in ...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Hohloch K,Zeynalova S,Chapuy B,Pfreundschuh M,Loeffler M,Ziepert M,Feller AC,Trümper L,Hasenclever D,Wulf G,Schmitz N

    更新日期:2016-06-01 00:00:00

  • Ixazomib-based regimens for relapsed/refractory multiple myeloma: are real-world data compatible with clinical trial outcomes? A multi-site Israeli registry study.

    abstract::Ixazomib, the first oral proteasome inhibitor (PI), has been approved for the treatment of relapsed refractory multiple myeloma (RRMM) in combination with lenalidomide and dexamethasone, based on the TOURMALINE-MM1 phase 3 trial, which demonstrated the efficacy and safety of this all-oral triplet, compared with lenali...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Cohen YC,Magen H,Lavi N,Gatt ME,Chubar E,Horowitz N,Kreiniz N,Tadmor T,Trestman S,Vitkon R,Rouvio O,Shvetz O,Shaulov A,Ziv-Baran T,Avivi I

    更新日期:2020-06-01 00:00:00

  • Neutralization of stem cell factor in vitro and in vivo: dose-dependent inhibition of stromal cells and induction of granulocyte/monocyte differentiation.

    abstract::Stem cell factor (SCF), also termed mast cell growth factor or c-kit ligand, plays a central role in the regulation of hematopoiesis and maintenance of viability of hematopoietic cells. We used a new murine monoclonal antibody (MAb) specific for canine SCF to further dissect the role of SCF in vitro and in vivo. This ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Huss R,Hong DS,Beckham C,Storb R,Broudy VC,Deeg HJ

    更新日期:1996-04-01 00:00:00