Modulation of rhodopsin function by properties of the membrane bilayer.


:A prevalent model for the function of rhodopsin centers on the metarhodopsin I (MI) to metarhodopsin II (MII) conformational transition as the triggering event for the visual process. Flash photolysis techniques enable one to determine the [MII]/[MI] ratio for rhodopsin in various recombinant membranes, and thus investigate the roles of the phospholipid head groups and the lipid acyl chains systematically. The results obtained to date clearly show that the pK for the acid-base MI-MII equilibrium of rhodopsin is modulated by the lipid environment. In bilayers of phosphatidylcholines the MI-MII equilibrium is shifted to the left; whereas in the native rod outer segment membranes it is shifted to the right, i.e., at neutral pH near physiological temperature. The lipid mixtures sufficient to yield full photochemical function of rhodopsin include a native-like head group composition, viz, comprising phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS), in combination with polyunsaturated docosahexaenoic acid (DHA; 22:6 omega 3) chains. Yet such a native-like lipid mixture is not necessary for the MI-MII conformational transition of rhodopsin; one can substitute other lipid compositions having similar properties. The MI-MII transition is favored by relatively small head groups which produce a condensed bilayer surface, viz, a comparatively small interfacial area as in the case of PE, together with bulky acyl chains such as DHA which prefer a relatively large cross sectional area. The resulting force imbalance across the layer gives rise to a curvature elastic stress of the lipid/water interface, such that the lipid mixtures yielding native-like behavior form reverse hexagonal (HII) phases at slightly higher temperatures. A relatively unstable membrane is needed: lipids tending to form the lamellar phase do not support full native-like photochemical function of rhodopsin. Thus chemically specific properties of the various lipids are not required, but rather average or material properties of the entire assembly, which may involve the curvature free energy of the membrane-lipid water interface. These findings reveal that the membrane lipid bilayer has a direct influence on the energetics of the conformational states of rhodopsin in visual excitation.


Chem Phys Lipids


Brown MF




Has Abstract


1994-09-06 00:00:00














  • Development and optimization of simultaneous determination of fat soluble vitamins by liquid chromatography tandem mass spectrometry.

    abstract:OBJECTIVE:Fat-soluble vitamins (A, D, E and K) are isoprene derived apolar molecules. While deficiencies of these vitamins have been associated with various diseases such as type 2 diabetes and cancer, high doses of Vitamin A and D can cause toxic effects. Accurate detection of serum levels of these vitamins have criti...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Ertugrul S,Yucel C,Sertoglu E,Ozkan Y,Ozgurtas T

    更新日期:2020-08-01 00:00:00

  • Structure of stratum corneum lipids characterized by FT-Raman spectroscopy and DSC. II. Mixtures of ceramides and saturated fatty acids.

    abstract::Fourier transform (FT) Raman spectroscopy and differential scanning calorimetry (DSC) were used to study the thermotropic phase behaviour of mixtures of ceramides type IV (CER) and stearic acid (SA). For comparison the melting behaviour of SA was re-examined. The Raman spectra of all mixtures in the solid state show s...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Neubert R,Rettig W,Wartewig S,Wegener M,Wienhold A

    更新日期:1997-09-24 00:00:00

  • Short-lived fluorescence component of DPH reports on lipid--water interface of biological membranes.

    abstract::Fluorescence measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) in large unilamellar phospholipid vesicles were performed to characterize the influence of the membrane physical properties on the short-lived lifetime component of the fluorescence decay. We have found that the short-lived component of DPH significantly...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Konopásek I,Vecer J,Strzalka K,Amler E

    更新日期:2004-07-01 00:00:00

  • Vitamin E - phosphatidylethanolamine interactions in mixed membranes with sphingomyelin: Studies by 2H NMR.

    abstract::Among the structurally diverse collection of lipids that comprise the membrane lipidome, polyunsaturated phospholipids are particularly vulnerable to oxidation. The role of α-tocopherol (vitamin E) is to protect this influential class of membrane phospholipid from oxidative damage. Whether lipid-lipid interactions pla...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cavazos AT,Kinnun JJ,Williams JA,Wassall SR

    更新日期:2020-09-01 00:00:00

  • Isothermal lipid phase transitions.

    abstract::In liotropic lipid systems phase transitions can be induced isothermally by changing the solvent concentration or composition; alternatively, lipid composition can be modified by (bio)chemical means. The probability for isothermal phase transitions increases with the decreasing transition entropy; it is proportional t...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Cevc G

    更新日期:1991-03-01 00:00:00

  • Molecular species analysis of lipids by metastable ion mass spectrometry.

    abstract::A convenient universal and fast mass spectrometrical method designed for the molecular species analysis of natural lipids is described. In contrast to the commonly employed procedures the method does not require chemical or enzymatic treatment and does not include chromatographic steps. The method relies on the recogn...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Batrakov SG,Sadovskaya VL,Rozynov BV,Bergelson LD

    更新日期:1983-11-01 00:00:00

  • The interaction of water with the phospholipid head group and its relationship to the lipid electrical conductivity.

    abstract::We have studied the interaction of water with the lipid head group by gravimetrically measuring the lipid water adsorption and the lateral dc electrical conductivity increase resulting from this hydration. We have done this for dimyristoyl phosphatidylcholine (DMPC) having protonated or deuterated hydrocarbon chains. ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Jendrasiak GL,Smith RL

    更新日期:2004-09-01 00:00:00

  • Membrane specificity of non-monotonic trends in cation-mediated injury to rapidly frozen phospholipid vesicles.

    abstract::Phospholipid vesicles like erythrocyte ghosts [1] have been shown to display trends in freeze-thaw injury with Group I ions which are non-monotonic in nature. That is to say, the relative extent of injury with such ions, measured as calcein release, does not follow a lyotropic series related solely to the hydrated ion...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Brearley CA,Hodges NA,Oliff CJ

    更新日期:1991-09-01 00:00:00

  • Biophysical perturbations induced by ethylazinphos in lipid membranes.

    abstract::Perturbations induced by ethylazinphos on the physical organization of dipalmitoylphosphatidylcholine (DPPC) and DPPC/cholesterol membranes were studied by differential scanning calorimetry (DSC) and fluorescence polarization of 2-, 6-, 12-(9-anthroyloxy) stearic acids and 16-(9-anthroyloxy) palmitic acid. Ethylazinph...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Videira RA,Antunes-Madeira MC,Madeira VM

    更新日期:1999-02-01 00:00:00

  • Bile acid signaling after an oral glucose tolerance test.

    abstract::Monitoring bile acids as signal molecules in combination with a bile acid synthesis marker and the FXR regulator fibroblast growth factor 19 (FGF19), this study addresses significant postprandial changes. The efficacy of the different pathways to regulate bile acid synthesis through short heterodimer partner (SHP) dep...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Matysik S,Martin J,Bala M,Scherer M,Schäffler A,Schmitz G

    更新日期:2011-09-01 00:00:00

  • Improvising anti-leishmanial activity of amphotericin B and paromomycin using co-delivery in d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) tailored nano-lipid carrier system.

    abstract::In the current study, we have focused on the design, development and in-vitro evaluation of d-α-tocopheryl polyethylene glycol 1000 succinate modified amphotericin B (AmB) and paromomycin (PM) loaded solid lipid nanoparticles (TPGS-SLNPs) by emulsion-solvent evaporation method. The optimized TPGS-SLNPs had a mean part...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Parvez S,Yadagiri G,Singh A,Karole A,Singh OP,Sundar S,Mudavath SL

    更新日期:2020-09-01 00:00:00

  • Inhibition of pain responses by activation of CB(2) cannabinoid receptors.

    abstract::Cannabinoid receptor agonists diminish responses to painful stimuli. Extensive evidence demonstrates that CB(1) cannabinoid receptor activation inhibits pain responses. Recently, the synthesis of CB(2) cannabinoid receptor-selective agonists has allowed testing whether CB(2) receptor activation inhibits pain. CB(2) re...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Malan TP Jr,Ibrahim MM,Vanderah TW,Makriyannis A,Porreca F

    更新日期:2002-12-31 00:00:00

  • The phase transition behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) model membrane influenced by 2,4-dichlorophenol--an FT-Raman spectroscopy study.

    abstract::The effect of 2,4-dichlorophenol (DCP) on the structures and phase transitions of fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes was studied using FT-Raman spectroscopy. Whereas the Raman frequency shifts of the most frequently investigated bands of C-C and C-H stretching regions only indi...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Csiszár A,Koglin E,Meier RJ,Klumpp E

    更新日期:2006-02-01 00:00:00

  • Influence of temperature on dynamic surface properties of spread DPPC monolayers in a broad range of surface pressures.

    abstract::This work is focused on the study of the dynamic surface properties of spread monolayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), which is the main component of the pulmonary surfactant (PS), in the region of high surface pressures and at different temperatures. The increase of temperature from 25 to 35 ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Bykov AG,Guzmán E,Rubio RG,Krycki MM,Milyaeva OY,Noskov BA

    更新日期:2019-12-01 00:00:00

  • Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichlorodihydrofluorescein (DCFH) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer.

    abstract::2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is commonly used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon. It has been suggested that DCFH-DA crosses the cell membrane, subsequently undergoing deacetylation by intracellula...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Afri M,Frimer AA,Cohen Y

    更新日期:2004-08-01 00:00:00

  • Lipid-protein interactions in human plasma LDL evidenced by magnetic resonance.

    abstract::Low density lipoprotein (LDL) particles exhibit extremely complex three-dimensional structural organization which is still not understood at the molecular level. The aim of this study was to provide the experimental evidence of a direct non-covalent interaction of the protein part with the lipid matrix. The approach w...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Kveder M,Marinić Z,Krisko A,Vikić-Topić D,Pifat G

    更新日期:2006-06-01 00:00:00

  • Aggregate formation in the intercalation of long-chain fatty acid esters into liposomes.

    abstract::Various hydrophobic benzenediacetic esters, the corresponding benzenedipropionic esters, and branched alkyl esters were intercalated into DMPC liposomes, where the molar ratio (n/n) of ester:DMPC was 1:5. In the case of the very long-chain derivatives, double carbonyl peaks were observed in the 13C NMR spectrum. This ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cohen Y,Afri M,Frimer AA

    更新日期:2008-10-01 00:00:00

  • Carbohydrate liquid crystals: synthesis and characterisation of the methyl-6-O-(n-acyl)-α-D-glucopyranosides.

    abstract::Seven members of the methyl-6-O-(n-acyl)-α-D-glucopyranosides have been synthesised and their transitional properties determined. The undecanoyl and octadecanoyl members do not exhibit liquid crystallinity while the members having chain lengths between dodecanoyl and hexadecanoyl exhibit a monotropic smectic A phase. ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cook AG,Wardell JL,Imrie CT

    更新日期:2011-02-01 00:00:00

  • The effect of dolichol on the permeability properties of phosphatidylcholine bilayers.

    abstract::The permeability of liposomes to water, glucose, Ca2+ and alkaline cations was monitored by recording the change in absorbance at 450 nm using a rapid reaction stopped-flow spectrophotometer. Liposomes were prepared with egg phosphatidylcholine and concentrations of dolichol ranging from 0.1% to 9% (w/w). Net permeabi...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Boscoboinik DO,Feliz M,Disalvo EA,Belocopitow E

    更新日期:1985-11-01 00:00:00

  • Tailoring lipid nanoconstructs for the oral delivery of paliperidone: Formulation, optimization and in vitro evaluation.

    abstract:PURPOSE:The present research work involves Quality by Design (QbD)-based fabrication of lipid nanoconstructs (LNC) of paliperidone (PPD) bearing superior biopharmaceutical attributes. METHODS:LNC of paliperidone was prepared by melt emulsification-probe sonication and high-pressure homogenization method followed by op...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Rehman S,Nabi B,Baboota S,Ali J

    更新日期:2021-01-01 00:00:00

  • Crystal structure of 3 beta-benzoyloxy-6 alpha-chloro-5 alpha-cholest-7-ene, a key intermediate in the chemical synthesis of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one.

    abstract::The X-ray crystal structure of 3 beta-benzoyloxy-6 alpha-chloro-5 alpha-cholest-7-ene (IV) was determined by the heavy atom method and refined to R = 0.063 (space group P21, a = 11.364, b = 11.089, c = 12.232, beta = 99.43 degrees, Z = 2). IV was previously shown to be an important intermediate in the acid-catalyzed i...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Wilson DK,Wilson WK,Quiocho FA,Schroepfer GJ Jr

    更新日期:1988-08-01 00:00:00

  • Double layer potential and degree of dissociation in charged lipid monolayers.

    abstract::One of the contributions to the surface potential in charged phospholipid monolayers at air-water interfaces is the double layer potential. In this note several misconceptions found in the literature concerning the relationship between the double layer potential and the degree of dissociation of the lipid polar headgr...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Aguilella VM,Mafé S,Manzanares JA

    更新日期:2000-04-01 00:00:00

  • Iron-catalyzed reaction products of alpha-tocopherol with 1-palmitoyl-2-linoleoyl-3-sn-phosphatidylcholine (13S)-hydroperoxide.

    abstract::Alpha-tocopherol was reacted with 1-palmitoyl-2-[(9Z,11E)-(S)-13-hydroperoxy-9,11-octadecadienoyl]-3-sn-phosphatidylcholine (13-PLPC-OOH) in the presence of a lipid-soluble iron chelate, Fe(III) acetylacetonate, in methanol at 37 degrees C. The reaction product was isolated and identified as a mixture of 1-palmitoyl-2...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Yamauchi R,Ozaki K,Shimoyamada M,Kato K

    更新日期:2002-02-01 00:00:00

  • Changes induced in bovine serum albumin following interactions with the lipid peroxidation product E-2-octenal.

    abstract::Bovine serum albumin (BSA) undergoes a number of deteriorative changes when exposed to E-2-octenal. Reaction of BSA with E-2-octenal produced fluorescent BSA with an excitation maximum at 350 nm and emission maximum at 440 nm and promoted polymerization. Amino acid analysis of the modified BSA showed that the E-2-octe...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Alaiz M,Barragán S

    更新日期:1995-08-25 00:00:00

  • Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.

    abstract::Phospholipases are ubiquitous in nature and the target of significant research aiming at both their physiological roles and technical applications in e.g. the food industry. In the search for sensitive and selective phospholipase assays, we have focused on synthetic FRET (Förster resonance energy transfer) substrates....

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Piel MS,Peters GH,Brask J

    更新日期:2017-01-01 00:00:00

  • Structural studies on branched fucosphingolipids of hog gastric mucosa.

    abstract::A structural studies have been performed on new complex glycolipids extracted from hog gastric mucosa by 0.4 M sodium acetate in methanol-chloroform-water, and which were purified to homogeneity by DEAE-Sephadex and Florisil column chromatography and by preparative thin-layer chromatography in three solvent systems. F...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Slomiany BL,Slomiany A

    更新日期:1977-09-01 00:00:00

  • Synthesis and characterization of long-chain 1,2-dioxo compounds.

    abstract::A series of long-chain methyl esters with vicinal oxo groups (1,2-diones; 1,2-diketones) were synthesized by potassium permanganate-based oxidation of methyl esters of mono-unsaturated fatty acids. The presence of two additional carbonyl groups may facilitate the synthesis of other derivatives. The starting materials ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Knothe G

    更新日期:2002-05-01 00:00:00

  • Arrangement of lipid and protein in human serum high density lipoproteins: a proposed model.

    abstract::Based on size and compositional data, models for the two major density subclasses of human serum high density lipoproteins have been developed. Descriptions of both the surface structure of the high density lipoproteins and the structure of their apolar core regions are integral parts of these models. The surface is d...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Verdery RB,Nichols AV

    更新日期:1975-04-01 00:00:00

  • Inhibitors of sterol synthesis. Metabolism of [2,4-3H]5 alpha-cholest-8(14)-en-3 beta-ol-15-one after oral administration to a nonhuman primate.

    abstract::5 alpha-Cholest-8(14)-en-3 beta-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to rodents and nonhuman primates. In the present study the metabolism of the 15-ketosterol has been investigated after the oral administration of a mix...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Pajewski TN,Brabson JS,Kisic A,Wang KS,Hylarides MD,Jackson EM,Schroepfer GJ Jr

    更新日期:1989-03-01 00:00:00

  • Self-assembled systems based on novel hydroxyethylated imidazolium-containing amphiphiles: Interaction with DNA decamer, protein and lipid.

    abstract::The study on aggregation capacity of novel imidazolium-containing amphiphiles of 1-(2-hydroxyethyl)alkylimidazolium bromide series and their interaction with bio-objects (DNA decamer, bovine serum albumin, phospholipid) was performed. It was revealed that introduction of hydroxyethyl moiety into the surfactant molecul...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Kuznetsova DA,Gabdrakhmanov DR,Lukashenko SS,Voloshina AD,Sapunova AS,Kashapov RR,Zakharova LY

    更新日期:2019-09-01 00:00:00