Proteomic approach to characterize the supramolecular organization of photosystems in higher plants.


:A project to investigate the supramolecular structure of photosystems was initiated, which is based on protein solubilizations by digitonin, protein separations by Blue native (BN)-polyacrylamide gel electrophoresis (PAGE) and protein identifications by mass spectrometry (MS). Under the conditions applied, nine photosystem supercomplexes could be described for chloroplasts of Arabidopsis, which have apparent molecular masses between 600 and 3200 kDa on BN gels. Identities of the supercomplexes were determined on the basis of their subunit compositions as documented by 2D BN/SDS-PAGE and BN/BN-PAGE. Two supercomplexes of 1060 and approximately 1600 kDa represent dimeric and trimeric forms of photosystem I (PSI), which include tightly bound LHCI proteins. Compared to monomeric PSI, these protein complexes are of low abundance. In contrast, photosystem II mainly forms part of dominant supercomplexes of 850, 1000, 1050 and 1300 kDa. According to our interpretation, these supercomplexes contain dimeric PSII, 1-4 LHCII trimers and additionally monomeric LHCII proteins. The 1300-kDa PSII supercomplex (containing four LHCII trimers) is partially converted into the 1000-kDa PSII supercomplex (containing two LHCII trimers) in the presence of dodecylmaltoside on 2D BN/BN gels. Analyses of peptides of the trypsinated 1300-kDa PSII supercomplex by mass spectrometry allowed to identify known subunits of the PSII core complex and additionally LHCII proteins encoded by eight different genes in Arabidopsis. Further application of this experimental approach will allow new insights into the supermolecular organization of photosystems in plants.






Heinemeyer J,Eubel H,Wehmhöner D,Jänsch L,Braun HP




Has Abstract


2004-06-01 00:00:00














  • Unusual cycloartane triterpenoids from Kadsura ananosma.

    abstract::Five 3,4-seco-cycloartane triterpenoids were isolated from the stems of Kadsura ananosma, two of which had rearranged 5/6 consecutive carbocycle rings C/D (trivially named ananosins A (1) and B (2)), one had a migrated CH3-18 (named ananosins C (3)), and two were analogs, ananosins D (4) and E (5). Their structures we...


    pub_type: 杂志文章


    authors: Yang JH,Pu JX,Wen J,Li XN,He F,Su J,Li Y,Sun HD

    更新日期:2015-01-01 00:00:00

  • Mulinane-type diterpenoids from Azorella compacta display antiplasmodial activity.

    abstract::Two mulinane-type diterpenoids were isolated from Azorella compacta; namely 20-hydroxymulin-11,13-dienyl acetate and 13,14-dihydroxymulin-11-en-20-oic acid. The structures were elucidated by analysis of their spectroscopic data. These compounds, as well as three previously isolated diterpenes, were evaluated as potent...


    pub_type: 杂志文章


    authors: Loyola LA,Bórquez J,Morales G,San-Martín A,Darias J,Flores N,Giménez A

    更新日期:2004-07-01 00:00:00

  • Abietane diterpenoids and triterpenoic acids from Salvia cilicica and their antileishmanial activities.

    abstract::Bioguided-fractionation of an acetone extract of the roots of Salvia cilicica (Lamiaceae) led to isolation of two new diterpenes, 7-hydroxy-12-methoxy-20-nor-abieta-1,5(10),7,9,12-pentaen-6,14-dione and abieta-8,12-dien-11,14-dione (12-deoxy-royleanone), together with oleanolic acid, ursolic acid, ferruginol, inuroyol...


    pub_type: 杂志文章


    authors: Tan N,Kaloga M,Radtke OA,Kiderlen AF,Oksüz S,Ulubelen A,Kolodziej H

    更新日期:2002-12-01 00:00:00

  • 13,14-dihydrocoptisine--the genuine alkaloid from Chelidonium majus.

    abstract::The genuine major benzylisoquinoline alkaloid occurring in the traditional medicinal plant greater celandine (Chelidonium majus L.) is 13,14-dihydrocoptisine and not - as described previously - coptisine. Structure of 13,14-dihydrocoptisine was elucidated. The discrepancy between the alkaloid pattern of the living pla...


    pub_type: 杂志文章


    authors: Paulsen J,Yahyazadeh M,Hänsel S,Kleinwächter M,Ibrom K,Selmar D

    更新日期:2015-03-01 00:00:00

  • Triterpenoids from the stems and leaves of Schisandra incarnata.

    abstract::Six undescribed triterpenoids, schincarins A‒F, categorized as the preschisanartane (schincarin A), 3,4-secocycloartane (schincarins B‒D), 3,4; 9,10-disecocycloartane (schincarin E), and 3,4-secolanostane (schincarin F) classes, along with twelve known analogues, were obtained from the stems and leaves of Schisandra i...


    pub_type: 杂志文章


    authors: Zhou M,Peng X,Zhou J,Liu Y,Meng X,Ruan H

    更新日期:2020-09-01 00:00:00

  • 5-Lipoxygenase-derived oxylipins from the red alga Rhodymenia pertusa.

    abstract::The lipid extract of the temperate red alga Rhodymenia pertusa has yielded four eicosanoid metabolites, three of which are new natural products. Using principally NMR and MS techniques, their structures were deduced as 5R,6S-dihydroxy-7(E),9(E),11(Z),14(Z)-eicosatetraenoic acid (5R,6S-diHETE), 5R*,6S*-dihydroxy-7(E),9...


    pub_type: 杂志文章


    authors: Jiang ZD,Ketchum SO,Gerwick WH

    更新日期:2000-01-01 00:00:00

  • Biosynthesis of 20-hydroxyecdysone in plants: 3β-hydroxy-5β-cholestan-6-one as an intermediate immediately after cholesterol in Ajuga hairy roots.

    abstract::3β-Hydroxy-5β-cholestan-6-one was identified in the EtOAc extract of Ajuga hairy roots by micro-analysis using LC-MS/MS in the multiple reaction mode (MRM). Furthermore, administration of (2,2,4,4,7,7-(2)H6)- and (2,2,4,4,6,7,7-(2)H7)-cholesterols to the hairy roots followed by LC-MS/MS analysis of the EtOAc extract o...


    pub_type: 杂志文章


    authors: Fujimoto Y,Maeda I,Ohyama K,Hikiba J,Kataoka H

    更新日期:2015-03-01 00:00:00

  • Isolation and structure elucidation of tetrameric procyanidins from unripe apples (Malus pumila cv. Fuji) by NMR spectroscopy.

    abstract::Procyanidins are plant secondary metabolites widely consumed and known to have various physiological functions, but their bioavailability and mechanism of action are still unclear especially for larger oligomers. One of the reasons is scarce information about the detailed structure of oligomeric procyanidins. As for a...


    pub_type: 杂志文章


    authors: Nakashima S,Oda C,Masuda S,Tagashira M,Kanda T

    更新日期:2012-11-01 00:00:00

  • Sebestenoids A-D, BACE1 inhibitors from Cordia sebestena.

    abstract::Bioassay-guided fractionation of an extract prepared from the fruits of Cordia sebestena led to the isolation of sebestenoids A-D (1-4). Their structures were elucidated on the basis of extensive NMR experiments and mass spectroscopic measurements. Compounds 1-4 exhibited moderate inhibition of the aspartic protease B...


    pub_type: 杂志文章


    authors: Dai J,Sorribas A,Yoshida WY,Williams PG

    更新日期:2010-12-01 00:00:00

  • Three types of sesquiterpenes from rhizomes of Atractylodes lancea.

    abstract::Eight sesquiterpenes, including four guaiane-types containing an interesting epoxy unit (1-4), a rare tricyclic carbon skeleton-type (5) and three eudesmane-types (6-8), along with five known compounds, were isolated from rhizomes of Atractylodes lancea. The structures and relative configurations of 1-8 were determine...


    pub_type: 杂志文章


    authors: Wang HX,Liu CM,Liu Q,Gao K

    更新日期:2008-07-01 00:00:00

  • Alkaloids from Cynanchum komarovii with inhibitory activity against the tobacco mosaic virus.

    abstract::A pyrroloisoquinoline alkaloid, 2,3-dimethoxy-6-(3-oxo-butyl)-7,9,10,11,11a,12-hexahydrobenzo[f]pyrrolo[1,2-b]isoquinoline (1), whose structure was determined by spectroscopic methods, was isolated from the aerial parts of Cynanchum komarovii, together with two known alkaloids, 7-demethoxytylophorine (2) and 7-demetho...


    pub_type: 杂志文章


    authors: An T,Huang RQ,Yang Z,Zhang DK,Li GR,Yao YC,Gao J

    更新日期:2001-12-01 00:00:00

  • Exudation of fluorescent beta-carbolines from Oxalis tuberosa L roots.

    abstract::Root fluorescence is a phenomenon in which roots of seedlings fluoresce when irradiated with ultraviolet (UV) light. Soybean (Glycine max) and rye grass (Elymus glaucus) are the only plant species that have been reported to exhibit this occurrence in germinating seedling roots. The trait has been useful as a marker in...


    pub_type: 杂志文章,收录出版


    authors: Bais HP,Park SW,Stermitz FR,Halligan KM,Vivanco JM

    更新日期:2002-11-01 00:00:00

  • A bidesmosidic triterpene saponin from Cephalaria transsylvanica.

    abstract::The structure of cephalaria saponin B, isolated from Cephalaria transsylvanica was elucidated as 3-O-([beta-D-xylopyranosyl(1-->4)-alpha-L-rhamnopyranosyl(1-->2)-beta-D- xylopyranosyl]-[28-O-beta-D-glucopyranosyl])-3beta,23-dihydroxy-12(13)-oleanene-28-oic acid. ...


    pub_type: 杂志文章


    authors: Alankuş-Calişkan O,Anil H

    更新日期:1995-04-01 00:00:00

  • Chondroprotective and anti-inflammatory effects of sesamin.

    abstract::Osteoarthritis (OA) is a major disability of elderly people. Sesamin is the main compound in Sesamun indicum Linn., and it has an anti-inflammatory effect by specifically inhibiting Δ5-desaturase in polyunsaturated fatty acid biosynthesis. The chondroprotective effects of sesamin were thus studied in a porcine cartila...


    pub_type: 杂志文章


    authors: Phitak T,Pothacharoen P,Settakorn J,Poompimol W,Caterson B,Kongtawelert P

    更新日期:2012-08-01 00:00:00

  • Comparative analysis of CYP93E proteins for improved microbial synthesis of plant triterpenoids.

    abstract::Cytochrome P450-dependent monooxygenases (P450s) belonging to the CYP93E subfamily catalyze the C-24 oxidation of the triterpene backbone during the biosynthesis of triterpenoid saponins, which are bioactive plant natural products. In our attempts to produce plant triterpenoids in the yeast Saccharomyces cerevisiae, w...


    pub_type: 杂志文章


    authors: Moses T,Thevelein JM,Goossens A,Pollier J

    更新日期:2014-12-01 00:00:00

  • Kahiricosides II-V, cycloartane glycosides from an Egyptian collection of Astragalus kahiricus.

    abstract::Four cycloartane-type saponins, kahiricosides II-V (1-4), were isolated from the aerial parts of Astragalus kahiricus of Egyptian origin. Their structures were established as 9beta,19-cyclolanost-24E-ene-3beta,6alpha,16beta,27-tetraol-3-O-beta-D-glucopyranoside, 9beta,19-cyclolanost-24E-ene-3beta,6alpha,16beta,27-tetr...


    pub_type: 杂志文章


    authors: Radwan MM,El-Sebakhy NA,Asaad AM,Toaima SM,Kingston DG

    更新日期:2004-11-01 00:00:00

  • Phenolic and triterpenoid glycosides from Aster batangensis.

    abstract::A new phenolic glycoside, asterbatanoside A [p-hydroxyacetophenone-4-O-beta-D-xylopyranosyl-(1-->6)-beta-D- glucopyranoside], and two new triterpenoid saponins, asterbatanoside B [2 alpha,3 beta,23-trihydroxyolean-12-en-28-oic acid-28-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside] and asterbatanoside C [3-O-b...


    pub_type: 杂志文章


    authors: Shao Y,Li YL,Zhou BN

    更新日期:1996-04-01 00:00:00

  • An acidic amino acid-specific protease from germinating soybeans.

    abstract::The degradation of the beta-conglycinin protein reserves in soybean seeds during germination and early growth begins with the proteolysis of its alpha and alpha' subunits by an enzyme called Protease C1. In the pathway, a number of proteolytic intermediates are produced and subsequently degraded. Determination of the ...


    pub_type: 杂志文章


    authors: Tan-Wilson AL,Liu X,Chen R,Qi X,Wilson KA

    更新日期:1996-05-01 00:00:00

  • Flavonoids from Ochna integerrima.

    abstract::Two biflavonoids namely, 2",3"-dihydroochnaflavone and 2",3"-dihydroochnaflavone 7"-O-methyl ether, and a flavonoid glycoside, 6-gamma,gamma-dimethylallyltaxifolin 7-O-beta-D-glucoside were isolated from the leaves of Ochna integerrima. ...


    pub_type: 杂志文章


    authors: Likhitwitayawuid K,Rungserichai R,Ruangrungsi N,Phadungcharoen T

    更新日期:2001-02-01 00:00:00

  • The structures of antioxidant and cytotoxic agents from natural source: anthraquinones and tannins from roots of Rumex patientia.

    abstract::A new anthraquinone glycoside, emodin-6-O-beta-D-glucopyranoside, and a new simple halogenated flavan-3-ol, 6-chlorocatechin, have been isolated from the roots of Rumex patientia L. together with seven known phenolic compounds. Their structures were elucidated on the basis of spectroscopic methods. Cytotoxic effects a...


    pub_type: 杂志文章


    authors: Demirezer LO,Kuruüzüm-Uz A,Bergere I,Schiewe HJ,Zeeck A

    更新日期:2001-12-01 00:00:00

  • Oleanane-type triterpene oligoglycosides with pancreatic lipase inhibitory activity from the pericarps of Sapindus rarak.

    abstract::The methanolic extract from the pericarps of Sapindus rarak DC. was found to show pancreatic lipase inhibitory activity (IC50=ca. 614 microg/mL). From the extract, oleanane-type triterpene oligoglycosides, rarasaponins I-III (1-3), and raraoside A (4), were isolated together with 13 known saponins and four known sesqu...


    pub_type: 杂志文章


    authors: Morikawa T,Xie Y,Asao Y,Okamoto M,Yamashita C,Muraoka O,Matsuda H,Pongpiriyadacha Y,Yuan D,Yoshikawa M

    更新日期:2009-06-01 00:00:00

  • Mechanistic insight in potential dual role of sinigrin against Helicoverpa armigera.

    abstract::The adverse effect of glucosinolates on diverse phytophagous insects is well documented, but its impact on insect physiology has remained enigmatic. Here we report insights into detrimental effects of plant glucosinolate molecule, sinigrin, on Helicoverpa armigera growth and development. In-silico screening of multipl...


    pub_type: 杂志文章


    authors: Agnihotri AR,Hulagabali CV,Adhav AS,Joshi RS

    更新日期:2018-01-01 00:00:00

  • Purification and characterization of a Ca(2+)-dependent protein kinase from sandalwood (Santalum album L.): evidence for Ca(2+)-induced conformational changes.

    abstract::An early development-specific soluble 55 kDa Ca(2+)-dependent protein kinase has been purified to homogeneity from sandalwood somatic embryos and biochemically characterized. The purified enzyme, swCDPK, resolved into a single band on 10% polyacrylamide gels, both under denaturing and non-denaturing conditions. swCDPK...


    pub_type: 杂志文章


    authors: Anil VS,Sankara Rao K

    更新日期:2001-09-01 00:00:00

  • Microbial transformation of papaveraldine.

    abstract::Preparative-scale fermentation of papaveraldine (1), the known benzylisoquinoline alkaloid, with Mucor ramannianus 1839 (sih) has resulted in a stereoselective reduction of the ketone group and the isolation of S-papaverinol (2) and S-papaverinol N-oxide (3). The structure elucidation of both metabolites was based pri...


    pub_type: 杂志文章


    authors: El Sayed KA

    更新日期:2000-03-01 00:00:00

  • Exclusive accumulation of Z-isomers of monolignols and their glucosides in bark of Fagus grandifolia.

    abstract::In addition to Z-coniferyl and Z-sinapyl alcohols, bark extracts of Fagus grandifolia also contain significant amounts of the glucosides, Z-coniferin, Z-isoconiferin (previously called faguside) and Z-syringin. The corresponding E-isomers of these glucosides do not accumulate to a detectable level. The accumulation ...


    pub_type: 杂志文章


    authors: Lewis NG,Inciong EJ,Ohashi H,Towers GH,Yamamoto E

    更新日期:1988-01-01 00:00:00

  • Does an apple a day keep the doctor away because a phytoestrogen a day keeps the virus at bay? A review of the anti-viral properties of phytoestrogens.

    abstract::From dengue to herpes and influenza to AIDS, the phytoestrogens that are present in many fruits and vegetables have been shown to exert anti-viral properties. Here we review the various different anti-viral mechanisms employed by phytoestrogens. ...


    pub_type: 杂志文章,评审


    authors: Martin JH,Crotty S,Warren P,Nelson PN

    更新日期:2007-02-01 00:00:00

  • Chemotaxonomy of Veroniceae and its allies in the Plantaginaceae.

    abstract::In a chemosystematic investigation of tribe Veroniceae (Plantaginaceae), representatives of Camptoloma, Sibthorpia, Veronica subg. Pentasepalae and subg. Hebe, Veronicastrum, Wulfenia, and the related Ellisiophyllum and Globularia were examined for non-flavonoid glycosides. From the 14 species studied, 28 different ir...


    pub_type: 杂志文章


    authors: Taskova RM,Gotfredsen CH,Jensen SR

    更新日期:2006-02-01 00:00:00

  • Metabolomic analysis applied to chemosystematics and evolution of megadiverse Brazilian Vernonieae (Asteraceae).

    abstract::Vernonia sensu lato is the largest and most complex genus of the tribe Vernonieae (Asteraceae). The tribe is chemically characterized by the presence of sesquiterpene lactones and flavonoids. Over the years, several taxonomic classifications have been proposed for Vernonia s.l. and for the tribe; however, there has be...


    pub_type: 杂志文章


    authors: Gallon ME,Monge M,Casoti R,Da Costa FB,Semir J,Gobbo-Neto L

    更新日期:2018-06-01 00:00:00

  • Macrocyclic trichothecenes are undetectable in kudzu (Pueraria montana) plants treated with a high-producing isolate of Myrothecium verrucaria.

    abstract::Myrothecium verrucaria was found to be an effective pathogen against kudzu grown in the greenhouse and the field. M. verrucaria produced large amounts of macrocyclic trichothecenes when cultured on solid rice medium, including epiroridin E (16.8 mg/g crude extract), epiisororidin E (1 mg/g), roridin E (8.7 mg/g), rori...


    pub_type: 杂志文章


    authors: Abbas HK,Tak H,Boyette CD,Shier WT,Jarvis BB

    更新日期:2001-09-01 00:00:00

  • Tumor promoting diterpenes from Euphorbia leuconeura L.

    abstract::Diterpene esters of the phorbol and ingenol types are known to be highly active tumor promoting agents that typically occur in members of the Euphorbiaceae. In the present work, Euphorbia leuconeura, a rare indoor plant, is analyzed for its tumor promoting potential. Latex as well as total leaf extracts exhibited Epst...


    pub_type: 杂志文章


    authors: Vogg G,Mattes E,Rothenburger J,Hertkorn N,Achatz S,Sandermann H Jr

    更新日期:1999-05-01 00:00:00