Mechanism of action of androgen in hair follicles.


:In order to investigate the mode of action of testosterone (T) on human hair follicles we studied the metabolism of T and localization of androgen receptors in outer root sheath cells (ORSC) and dermal papilla cells (DPC) from different body sites. T was principally metabolized to androstenedione (delta 4) even in beard ORSC as well as epidermal keratinocytes (EK), and the ratio of apparent 5 alpha-reductase (5 alpha-R) to 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) did not differ between these two kinds of cells. The 5 alpha-R activity in beard DPC was 3 times as high as that in occipital scalp and axillary DPC. The 5 alpha-R of beard DPC exhibited a narrow optimum pH of 5.5, which is characteristic of type 2 enzyme present in androgen target cells. In contrast, 5 alpha-R of DPC from axillary and occipital scalp hair showed a broad optimum pH range between 6.5-9.0 corresponding to type 1 5 alpha-R. Androgen receptors were detected in the DPC of beard and axillary hair follicles, but not in those of occipital scalp hair follicles using immunohistochemical staining with polyclonal anti-androgen receptor antibody. Epithelial cells of the hair bulb were not stained by the antibody. Androgen receptors were also detected in the nuclei of cultured beard and axillary DPC, but the DPC from occipital scalp hair follicles showed little staining with the antibody. We also examined the effects of T on the DNA synthesis and proliferation of cultured ORSC and DPC. T did not have a proliferative effect on either type of cell when cultured alone.(ABSTRACT TRUNCATED AT 250 WORDS)


J Dermatol Sci


Itami S,Sonoda T,Kurata S,Takayasu S




Has Abstract


1994-07-01 00:00:00










7 Suppl


  • European ancestry and polymorphisms in DNA repair genes modify the risk of melanoma: a case-control study in a high UV index region in Brazil.

    abstract:BACKGROUND:UV radiation is the major environmental factor related to development of cutaneous melanoma. Besides sun exposure and the influence of latitude, some host characteristics such as skin phototype and hair and eye color are also risk factors for melanoma. Polymorphisms in DNA repair genes could be good candidat...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Gonçalves FT,Francisco G,de Souza SP,Luiz OC,Festa-Neto C,Sanches JA,Chammas R,Gattas GJ,Eluf-Neto J

    更新日期:2011-10-01 00:00:00

  • A unique monoclonal antibody 29A stains the cytoplasm of amniotic epithelia and cutaneous basement membrane.

    abstract:BACKGROUND:The basic function of epithelia is to provide a boundary between tissue and its external environment, and is achieved by a wide variety of components including extracellular molecules. Multiple monoclonal antibodies raised against epithelial antigens have helped identify a range of distinct, novel protein ep...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Ota M,Sawamura D,Yokota K,Ueda M,Horiguchi Y,Kodama K,Goto M,Shimizu H

    更新日期:2006-04-01 00:00:00

  • Characterization of itch-associated responses of NC mice with mite-induced chronic dermatitis.

    abstract::To clarify the behavioral and pathological features of spontaneous scratching of NC mice with mite-induced chronic dermatitis, we investigated the spontaneous and pruritogen-evoked scratching of NC mice. Although the frequency of scratching of NC mouse did not increase under specific pathogen-free environment, it grad...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Yamaguchi T,Maekawa T,Nishikawa Y,Nojima H,Kaneko M,Kawakita T,Miyamoto T,Kuraishi Y

    更新日期:2001-01-01 00:00:00

  • Glycolic acid induces keratinocyte proliferation in a skin equivalent model via TRPV1 activation.

    abstract:BACKGROUND:Glycolic acid (GA) is the most commonly used alpha-hydroxy acid (AHA) for dermatologic applications, and is considered as a versatile superficial peeling agent for facial rejuvenation. Its therapeutic effect includes acceleration of epidermal turnover without apparent inflammation, and its action is pH-depen...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Denda S,Denda M,Inoue K,Hibino T

    更新日期:2010-02-01 00:00:00

  • Keratinocyte-derived TGFβ is not required to maintain skin immune homeostasis.

    abstract:BACKGROUND:Transforming growth factor beta 1 (TGFβ) is known to be a regulator of autoimmunity. Loss of TGFβ leads to severe multi-organ autoimmunity in mice. In skin, role of TGFβ in suppressing autoimmunity is unclear. OBJECTIVE:Determine whether Keratinocyte (KC)-derived TGFβ is required for skin immune homeostasis...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Yang Y,Zenke Y,Hirai T,Kaplan DH

    更新日期:2019-05-01 00:00:00

  • Serum concentration of the soluble interleukin-2 receptor in vitiligo patients.

    abstract::Serum levels of sIL-2R can be used to monitor in vivo immune activation and its elevation have been shown to be correlated with T cell mediated immune disease such as atopic dermatitis, psoriasis, lymphoma and systemic sclerosis. Vitiligo is the disease of depigmentation caused by destruction of melanocytes, and there...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Yeo UC,Yang YS,Park KB,Sung HT,Jung SY,Lee ES,Shin MH

    更新日期:1999-04-01 00:00:00

  • Induction of MHC class II antigen expression on human HMC-1 mast cells.

    abstract::Mast cells are not only the primary effector cells of immediate type immune reactions, but they have recently also been considered to contribute to the induction of an immune response. Data on the ability of the cells to express major histocompatibility complex (MHC) antigens and the mechanisms involved are however co...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Grabbe J,Karau L,Welker P,Ziegler A,Henz BM

    更新日期:1997-11-01 00:00:00

  • Activation of STING signaling accelerates skin wound healing.

    abstract:BACKGROUND:The process of repair after skin injury is precisely regulated by a variety of mediators such as cytokines and chemokines. Recent reports demonstrated that cytoplasmic DNA-sensor cyclic GMP-AMP synthase (cGAS) activates the stimulator of interferon genes (STING) via production of cyclic GMP-AMP (cGAMP) and s...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Mizutani Y,Kanbe A,Ito H,Seishima M

    更新日期:2020-01-01 00:00:00

  • Cutaneous manifestations of COVID-19: Report of three cases and a review of literature.

    abstract:BACKGROUND:Various cutaneous manifestations have been observed in patients with COVID-19 infection. However, overall similarities in the clinical presentation of these dermatological manifestations have not yet been summarized. OBJECTIVE:This review aims to provide an overview of various cutaneous manifestations in pa...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Sachdeva M,Gianotti R,Shah M,Bradanini L,Tosi D,Veraldi S,Ziv M,Leshem E,Dodiuk-Gad RP

    更新日期:2020-05-01 00:00:00

  • Streptococci isolated from various skin lesions: the interaction with Staphylococcus aureus strains.

    abstract::We isolated 73 streptococcus strains (41 from infections, and 32 from colonization) from various skin diseases between March, 1994, and June, 1998. In 29 out of 41 cases of infective origin, Staphylococcus aureus strains were simultaneously isolated. Twenty-four out of 28 patients with impetigo were suffering from ato...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Akiyama H,Yamasaki O,Kanzaki H,Tada J,Arata J

    更新日期:1999-01-01 00:00:00

  • Regulation of keratinocyte differentiation by O-GlcNAcylation.

    abstract:BACKGROUND:O-linked β-N-acetylglucosamine (O-GlcNAc) modification is one of the posttranslational modification, emerging as an important regulatory mechanism in various cellular events. OBJECTIVE:We attempted to investigate whether O-GlcNAcylation is involved in keratinocyte differentiation. METHODS:Immunohistochemis...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Sohn KC,Lee EJ,Shin JM,Lim EH,No Y,Lee JY,Yoon TY,Lee YH,Im M,Lee Y,Seo YJ,Lee JH,Kim CD

    更新日期:2014-07-01 00:00:00

  • rAAV2-mediated restoration of LEKTI in LEKTI-deficient cells from Netherton patients.

    abstract:BACKGROUND:Netherton syndrome (NS, MIM 256500) is a potential live threatening autosomal-recessive skin disorder clinically characterized by the trias of congenital erythroderma, hair shaft anomalies and atopic diathesis. It is caused by mutations in the gene SPINK5 resulting in a deficiency of its processed protein na...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Roedl D,Oji V,Buters JT,Behrendt H,Braun-Falco M

    更新日期:2011-03-01 00:00:00

  • Malignant blue nevus: a report of eight cases.

    abstract::Malignant blue nevus is uncommon compared to its benign counterpart and is regarded as a rare form of malignant melanoma. We report the clinical and histological findings in eight cases. Histologically, all eight specimens showed no epidermal involvement and had contained within or were adjacent to portions of blue ne...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Mehregan DA,Gibson LE,Mehregan AH

    更新日期:1992-11-01 00:00:00

  • Sirt1 ameliorates systemic sclerosis by targeting the mTOR pathway.

    abstract:BACKGROUND:Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by inflammation and fibrosis. Our previous research has indicated that Sirtuin1 (Sirt1) plays a role in the regulation of TNF-α-induced inflammation; however, whether Sirt1 may inhibit the progress of SSc by blocking inflammation remains ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Zhu X,Chu H,Jiang S,Liu Q,Liu L,Xue Y,Zheng S,Wan W,Qiu J,Wang J,Zou H

    更新日期:2017-08-01 00:00:00

  • Gene expression in canine atopic dermatitis and correlation with clinical severity scores.

    abstract:BACKGROUND:Canine atopic dermatitis (cAD) is a common condition in dogs that may be a naturally occurring model for human atopic dermatitis (hAD). Despite this, comparative research is limited, particularly into the genetic background of cAD. OBJECTIVES:1. Measure candidate gene expression in cAD skin using quantitati...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Wood SH,Clements DN,Ollier WE,Nuttall T,McEwan NA,Carter SD

    更新日期:2009-07-01 00:00:00

  • Toxic epidermal necrolysis and Stevens-Johnson syndrome: soluble Fas ligand involvement in the pathomechanisms of these diseases.

    abstract::Toxic epidermal necrolysis (TEN) and Stevens-Johnson syndrome (SJS) are considered part of a spectrum of adverse cutaneous drug reactions showing severe and extensive skin detachment. TEN and SJS are morphologically characterized by active apoptotic keratinocyte cell death that results in the separation of the epiderm...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Abe R

    更新日期:2008-12-01 00:00:00

  • Major cleavage-dependent epitopes for linear IgA bullous dermatosis are formed at the boundary between the non-collagenous 16A and collagenous 15 domains of BP180.

    abstract:BACKGROUND:The autoantigen for the major type of linear IgA bullous dermatosis (LAD, lamina lucida type) is the shed ectodomain of BP180. However, it is unknown why most LAD sera react with the shed ectodomain but not with the intact BP180/type XVII collagen. OBJECTIVE:The aim of this study was to characterize the uni...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Yamauchi T,Matsushita S,Hashimoto T,Hirako Y

    更新日期:2014-10-01 00:00:00

  • Attenuation of UVA-induced damage to human keratinocytes by silymarin.

    abstract:BACKGROUND:UV radiation from sunlight is a potent environmental risk factor in skin cancer pathogenesis. UVA is the major portion of UV light reaching the earth surface ( approximately 95%) and it is reported to lead to benign and malignant tumor formation. UVA-mediated cellular damage occurs primarily through the rele...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Svobodová A,Zdarilová A,Malisková J,Mikulková H,Walterová D,Vostalová J

    更新日期:2007-04-01 00:00:00

  • Genetically engineered mouse models for skin research: taking the next step.

    abstract::Genetically engineered mouse models are invaluable to investigators in nearly all areas of biomedical research. The use of genetically engineered mice has allowed researchers to explore fundamental functions of genes in a mammal that shares substantial similarities with human physiology and pathology. Genetically engi...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Chen J,Roop DR

    更新日期:2008-10-01 00:00:00

  • Genetic predisposition to bullous pemphigoid.

    abstract::Bullous pemphigoid (BP) is a common autoimmune blistering skin disease that mainly affects elderly patients. Although BP risk is strongly influenced by age, genetic factors are also important determinants of this disease. Many genomic regions, especially in the HLA-II region, have been found to influence BP susceptibi...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Zhang J,Wang G

    更新日期:2020-11-01 00:00:00

  • Purification of human blood eosinophils by a combination method using anti-CD16 monoclonal antibody, immunobeads, and Nycodenz density gradient.

    abstract::A simple method is described for the procurement of human blood eosinophil phenotypes by combining an anti-CD16 monoclonal antibody, immunobeads, and a non-toxic and non-ionic density gradient medium, Nycodenz. The purification depends on the removal of mononuclear cells using a 1.076/1.102 g/ml Nycodenz density gradi...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Miyasato M,Tsuda S,Kitamura N,Shirouzu K,Nakama T,Sasai Y

    更新日期:1995-09-01 00:00:00

  • Spatial data analysis by epidermal Langerhans cells reveals an elegant system.

    abstract::Langerhans cells are dendritic cells situated in the mammalian epidermis. In human epidermis, the concentration is between 460 and 1000 mm(-2). Langerhans cells fulfill an essential role in skin immune responses. Numerous scientific reports on Langerhans cells have appeared, but with no systematic research on the patt...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Numahara T,Tanemura M,Nakagawa T,Takaiwa T

    更新日期:2001-04-01 00:00:00

  • The antimicrobial peptide derived from insulin-like growth factor-binding protein 5, AMP-IBP5, regulates keratinocyte functions through Mas-related gene X receptors.

    abstract:BACKGROUND:In addition to their microbicidal properties, host defense peptides (HDPs) display various immunomodulatory functions, including keratinocyte production of cytokines/chemokines, proliferation, migration and wound healing. Recently, a novel HDP named AMP-IBP5 (antimicrobial peptide derived from insulin-like g...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Chieosilapatham P,Niyonsaba F,Kiatsurayanon C,Okumura K,Ikeda S,Ogawa H

    更新日期:2017-10-01 00:00:00

  • Exploring the imbalance of circulating follicular helper CD4+ T cells in sarcoidosis patients.

    abstract:BACKGROUND:Sarcoidosis is a systemic granulomatous disease characterized by the combination of Th1 and Th17 responses. Recently, several arguments have suggested a potential involvement of B cells as well as T cells in the pathogenesis of sarcoidosis. Follicular helper CD4+ T (TFH) cells are specialized in interacting ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Ly NTM,Ueda-Hayakawa I,Nguyen CTH,Okamoto H

    更新日期:2020-03-01 00:00:00

  • Activation of fibroblast proliferation by Werner's syndrome fibroblast-conditioned medium.

    abstract::The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late pass...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Hatamochi A,Arakawa M,Takeda K,Ueki H

    更新日期:1994-06-01 00:00:00

  • Culture of human outer root sheath cells from plucked hair follicles in serum-free conditions.

    abstract::We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be seria...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Arase S,Katoh S,Sadamoto Y,Nakanishi H,Urano Y,Fujie K,Takeda K

    更新日期:1991-01-01 00:00:00

  • Combined treatment with H1 and H4 receptor antagonists reduces inflammation in a mouse model of atopic dermatitis.

    abstract:BACKGROUND:Histamine 4 receptor (H4R) antagonists are considered as new therapeutics for the treatment of atopic dermatitis (AD) and first clinical trials have already shown promising results. Histamine 1 receptor (H1R) antagonists are traditionally used to treat AD although the evidence for the efficacy is weak. The c...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Köchling H,Schaper K,Wilzopolski J,Gutzmer R,Werfel T,Bäumer W,Kietzmann M,Rossbach K

    更新日期:2017-08-01 00:00:00

  • CCR7 expression correlates with subcutaneous involvement in mycosis fungoides skin lesions and promotes migration of mycosis fungoides cells (MyLa) through mTOR activation.

    abstract:BACKGROUND:The molecular pathogenesis of mycosis fungoides (MF) is currently poorly understood. The chemokine receptor CCR7 has been demonstrated to be involved in the development and progression of certain cancers, but its role in MF has rarely been investigated. OBJECTIVES:We seek to determine whether CCR7 is expres...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Hu SC,Lin CL,Hong CH,Yu HS,Chen GS,Lee CH

    更新日期:2014-04-01 00:00:00

  • Harlequin ichthyosis and other autosomal recessive congenital ichthyoses: the underlying genetic defects and pathomechanisms.

    abstract::Autosomal recessive congenital ichthyoses (ARCI) include several severe subtypes including harlequin ichthyosis (HI), lamellar ichthyosis and non-bullous congenital ichthyosiform erythroderma. Patients with these severe types of ichthyoses frequently show severe hyperkeratosis and scales over a large part of the body ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审


    authors: Akiyama M

    更新日期:2006-05-01 00:00:00

  • Keratinocyte growth factor and keratin gene regulation.

    abstract::Keratinocyte growth factor (KGF) is a stromally derived paracrine mitogen that belongs to the fibroblast growth factor (FGF) family. It is secreted by dermal fibroblasts and specifically promotes keratinocyte proliferation. We have recently shown that epidermal growth factor (EGF) and transforming growth factor beta (...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章


    authors: Latkowski JM,Freedberg IM,Blumenberg M

    更新日期:1995-01-01 00:00:00