Nuclear localization signals, but not putative leucine zipper motifs, are essential for nuclear transport of hepatitis delta antigen.


:Hepatitis delta antigen (HDAg) is the only known protein of hepatitis delta virus and was previously shown to localize in the nucleoplasm of infected liver cells. In this study, nuclear localization signals of HDAg were defined by expressing various domains of the antigen in both hepatic and nonhepatic cells as beta-galactosidase fusion proteins. A cytochemical staining assay demonstrated that a domain from amino acid residues 35 to 88 of HDAg was able to facilitate transport to the nucleus of the originally cytoplasm-localized protein beta-galactosidase. Two nuclear localization signals, NLS1 and NLS2, which are similar to those of simian virus 40 T antigen and polyomavirus T antigen, respectively, were identified. Either NLS1 or NLS2 alone was sufficient for the nuclear transport of HDAg. However, a fusion protein (N65Z) containing beta-galactosidase and the N-terminal 65 amino acids of HDAg, containing NLS1, was localized exclusively in the cytoplasm and perinuclear region. A possible hydrophobic subdomain between amino acid residues 50 and 65 may block the function of NLS1. Nevertheless, N65Z could enter the nuclei of transfected cells when it was coexpressed with full-length HDAg. Entry into the nucleus may be mediated by the coiled-coil structure rather than the putative leucine zipper motif located between amino acid residues 35 and 65. The existence of two independent nuclear localization signals may ensure the proper functioning of HDAg in the multiplication of delta virus in the nucleus. In addition, two putative casein kinase II sites (SRSE-5 and SREE-126) that may be important in controlling the rate of nuclear transport were found in HDAg.


J Virol


Journal of virology


Chang MF,Chang SC,Chang CI,Wu K,Kang HY




Has Abstract


1992-10-01 00:00:00












  • Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.

    abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lee PS,Zhu X,Yu W,Wilson IA

    更新日期:2015-07-01 00:00:00

  • Aerosol transmission of chronic wasting disease in white-tailed deer.

    abstract::While the facile transmission of chronic wasting disease (CWD) remains incompletely elucidated, studies in rodents suggest that exposure of the respiratory mucosa may be an efficient pathway. The present study was designed to address this question in the native cervid host. Here, we demonstrate aerosol transmission of...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Denkers ND,Hayes-Klug J,Anderson KR,Seelig DM,Haley NJ,Dahmes SJ,Osborn DA,Miller KV,Warren RJ,Mathiason CK,Hoover EA

    更新日期:2013-02-01 00:00:00

  • Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type 2.

    abstract::The nonpathogenic human defective parvovirus adeno-associated virus (AAV) type 2 induced differentiation-associated antigens in cells of the human leukemia cell line HL60 (CD 67), as well as in two different lines of immortalized human keratinocytes, HaCaT and HPK Ia cells (involucrin and cytokeratin 10). Simultaneous...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Klein-Bauernschmitt P,zur Hausen H,Schlehofer JR

    更新日期:1992-07-01 00:00:00

  • Structure-based rationale for the rescue of systemic movement of brome mosaic virus by spontaneous second-site mutations in the coat protein gene.

    abstract::We describe spontaneous second-site reversions within the coat protein open reading frame that rescue the systemic-spread phenotype and increase virion stability of a mutant of brome mosaic virus. Based on the crystal structure of the related cowpea chlorotic mottle virus, we show that the modified residues are spatia...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Flasinski S,Dzianott A,Speir JA,Johnson JE,Bujarski JJ

    更新日期:1997-03-01 00:00:00

  • Recruitment of herpes simplex virus type 1 immediate-early protein ICP0 to the virus particle.

    abstract::Although the herpes simplex virus type 1 (HSV-1) tegument is comprised of a large number of viral and cellular proteins, how and where in the cell these proteins are recruited into the virus structure is poorly understood. We have shown previously that the immediate-early gene product ICP0 is packaged by a mechanism d...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Maringer K,Elliott G

    更新日期:2010-05-01 00:00:00

  • Inactivation of Genes by Frameshift Mutations Provides Rapid Adaptation of an Attenuated Vaccinia Virus.

    abstract::Unlike RNA viruses, most DNA viruses replicate their genomes with high-fidelity polymerases that rarely make base substitution errors. Nevertheless, experimental evolution studies have revealed rapid acquisition of adaptive mutations during serial passage of attenuated vaccinia virus (VACV). One way in which adaptatio...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Senkevich TG,Zhivkoplias EK,Weisberg AS,Moss B

    更新日期:2020-08-31 00:00:00

  • Infection by coronavirus JHM of rat neurons and oligodendrocyte-type-2 astrocyte lineage cells during distinct developmental stages.

    abstract::Primary telencephalic cultures derived from neonatal Wistar Furth rats were able to support the growth of coronavirus JHM if a viable neuronal population was maintained. This occurred under serum-free defined, but not serum-supplemented, growth conditions. The importance of neurons in establishing infections in mixed ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pasick JM,Dales S

    更新日期:1991-09-01 00:00:00

  • Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA.

    abstract::Oligonucleotide-directed mutagenesis was used to construct a nonsense mutation in open reading frame (ORF) E2 of bovine papillomavirus DNA. A single base substitution mutation was constructed which converted a TAC codon into a TAG amber stop codon at a position in the ORF that did not overlap with any other viral ORFs...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: DiMaio D

    更新日期:1986-02-01 00:00:00

  • Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15.

    abstract::Budding of retroviruses from cell membranes requires ubiquitination of Gag and recruitment of cellular proteins involved in endosome sorting, including endosome sorting complex required for transport III (ESCRT-III) protein complex and vacuolar protein sorting 4 (VPS4) and its ATPase. In response to infection, a cellu...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kuang Z,Seo EJ,Leis J

    更新日期:2011-07-01 00:00:00

  • Immunoreceptor tyrosine-based activation motif-dependent signaling by Kaposi's sarcoma-associated herpesvirus K1 protein: effects on lytic viral replication.

    abstract::The Kaposi's sarcoma-associated herpesvirus (KSHV) K1 gene encodes a polypeptide bearing an immunoreceptor tyrosine-based activation motif (ITAM) that is constitutively active for ITAM-based signal transduction. Although ectopic overexpression of K1 in cultured fibroblasts can lead to growth transformation, in vivo th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lagunoff M,Lukac DM,Ganem D

    更新日期:2001-07-01 00:00:00

  • Mutations in the membrane-proximal region of the influenza A virus M2 protein cytoplasmic tail have modest effects on virus replication.

    abstract::Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane-proximal residues in the M2 cytoplasmic tail in virus replication, we utilized scanning and directed alanine mutagenesis in combination wit...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Stewart SM,Pekosz A

    更新日期:2011-12-01 00:00:00

  • Properties of a ribonucleoprotein particle isolated from Nonidet P-40-treated Rous sarcoma virus.

    abstract::A ribonucleoprotein particle containing about 20% ribonucleic acid (RNA), and containing little if any phospholipid or glucosamine, was recovered in high yield after treatment of Schmidt-Ruppin strain of Rous sarcoma virus and B77 virus with the nonionic detergent Nonidet P-40. This structure, which probably derives f...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Davis NL,Rueckert RR

    更新日期:1972-11-01 00:00:00

  • Differential effects of human immunodeficiency virus type 1 Vpu on the stability of BST-2/tetherin.

    abstract::BST-2/CD317/tetherin is a host factor that inhibits the release of HIV-1 and other unrelated viruses. A current model proposes that BST-2 physically tethers virions to the surface of virus-producing cells. The HIV-1-encoded Vpu protein effectively antagonizes the activity of BST-2. How Vpu accomplishes this task remai...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Andrew AJ,Miyagi E,Strebel K

    更新日期:2011-03-01 00:00:00

  • The TLR4-TRIF pathway protects against H5N1 influenza virus infection.

    abstract::Prestimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked out. Microarray analysis of primary mouse lung cultur...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shinya K,Ito M,Makino A,Tanaka M,Miyake K,Eisfeld AJ,Kawaoka Y

    更新日期:2012-01-01 00:00:00

  • Interaction of NS2 with AIMP2 facilitates the switch from ubiquitination to SUMOylation of M1 in influenza A virus-infected cells.

    abstract:UNLABELLED:Influenza A viruses (IAVs) rely on host factors to support their life cycle, as viral proteins hijack or interact with cellular proteins to execute their functions. Identification and understanding of these factors would increase our knowledge of the molecular mechanisms manipulated by the viruses. In this s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gao S,Wu J,Liu RY,Li J,Song L,Teng Y,Sheng C,Liu D,Yao C,Chen H,Jiang W,Chen S,Huang W

    更新日期:2015-01-01 00:00:00

  • Open reading frame 33 of a gammaherpesvirus encodes a tegument protein essential for virion morphogenesis and egress.

    abstract::Tegument is a unique structure of herpesvirus, which surrounds the capsid and interacts with the envelope. Morphogenesis of gammaherpesvirus is poorly understood due to lack of efficient lytic replication for Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8, which are etiologically as...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Guo H,Wang L,Peng L,Zhou ZH,Deng H

    更新日期:2009-10-01 00:00:00

  • Assembly of vaccinia virus: effects of rifampin on the intracellular distribution of viral protein p65.

    abstract::The cytoplasmic assembly of vaccinia virus is reversibly blocked by the antibiotic rifampin, leading to the accumulation of partially membrane-delineated rifampin bodies in infected cells. Rifampin-resistant vaccinia virus mutants have point mutations in the D13L gene, which is controlled by a late promoter and expres...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sodeik B,Griffiths G,Ericsson M,Moss B,Doms RW

    更新日期:1994-02-01 00:00:00

  • Asymmetric transcription of mouse mammary tumor virus genes in vivo and in vitro.

    abstract::Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of n...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Prewitt RS,Washington LD,Stallcup MR

    更新日期:1984-04-01 00:00:00

  • Thermostability of the Foot-and-Mouth Disease Virus Capsid Is Modulated by Lethal and Viability-Restoring Compensatory Amino Acid Substitutions.

    abstract::Infection by viruses depends on a balance between capsid stability and dynamics. This study investigated biologically and biotechnologically relevant aspects of the relationship in foot-and-mouth disease virus (FMDV) between capsid structure and thermostability and between thermostability and infectivity. In the FMDV ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: López-Argüello S,Rincón V,Rodríguez-Huete A,Martínez-Salas E,Belsham GJ,Valbuena A,Mateu MG

    更新日期:2019-05-01 00:00:00

  • The I domain is required for efficient plasma membrane binding of human immunodeficiency virus type 1 Pr55Gag.

    abstract::The interaction of the human immunodeficiency virus type 1 (HIV-1) Pr55Gag molecule with the plasma membrane of an infected cell is an essential step of the viral life cycle. Myristic acid and positively charged residues within the N-terminal portion of MA constitute the membrane-binding domain of Pr55Gag. A separate ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sandefur S,Varthakavi V,Spearman P

    更新日期:1998-04-01 00:00:00

  • Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L.

    abstract::Viruses in the family Coronaviridae, within the order Nidovirales, are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans. These viruses encode conserved replicase and structural proteins as well as more diverse accessory proteins, encoded in the 3' ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Goldstein SA,Thornbrough JM,Zhang R,Jha BK,Li Y,Elliott R,Quiroz-Figueroa K,Chen AI,Silverman RH,Weiss SR

    更新日期:2017-02-14 00:00:00

  • Active-site mutations in the South african human immunodeficiency virus type 1 subtype C protease have a significant impact on clinical inhibitor binding: kinetic and thermodynamic study.

    abstract::Human immunodeficiency virus (HIV) infections in sub-Saharan Africa represent about 56% of global infections. Study of active-site mutations (the V82A single mutation and the V82F I84V double mutation) in the less-studied South African HIV type 1 subtype C (C-SA) protease indicated that neither mutation had a signific...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mosebi S,Morris L,Dirr HW,Sayed Y

    更新日期:2008-11-01 00:00:00

  • Vesicular stomatitis virus NS proteins: structural similarity without extensive sequence homology.

    abstract::The complete nucleotide sequence of the NS mRNA of vesicular stomatitis virus (New Jersey serotype) was established from two cDNA clones spanning the entire coding region of the mRNA. The gene is 856 nucleotides long and can code for a polypeptide of 274 amino acids. Comparison with the nucleotide sequence of the NS g...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gill DS,Banerjee AK

    更新日期:1985-07-01 00:00:00

  • Mutational analysis of the central domain of adenovirus virus-associated RNA mandates a revision of the proposed secondary structure.

    abstract::Protein synthesis in adenovirus-infected cells is regulated during the late phase of infection. The rate of initiation is maintained by a small viral RNA, virus-associated (VA) RNAI, which prevents the phosphorylation of eukaryotic initiation factor eIF-2 by a double-stranded RNA-activated protein kinase, DAI. On the ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pe'ery T,Mellits KH,Mathews MB

    更新日期:1993-06-01 00:00:00

  • Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.

    abstract::Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Neary K,Horwitz BH,DiMaio D

    更新日期:1987-04-01 00:00:00

  • Herpes Simplex Virus 1 UL36USP Antagonizes Type I Interferon-Mediated Antiviral Innate Immunity.

    abstract::Type I interferons (IFNs), as major components of the innate immune system, play a vital role in host resistance to a variety of pathogens. Canonical signaling mediated by type I IFNs activates the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway through binding to the IFN-α/β receptor ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yuan H,You J,You H,Zheng C

    更新日期:2018-09-12 00:00:00

  • Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus.

    abstract::West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent or treat disease. Passive immunization with antibodies against the envelope protein represents a promising means to provide short-term prophylaxis and treatment for West Nile virus infect...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gould LH,Sui J,Foellmer H,Oliphant T,Wang T,Ledizet M,Murakami A,Noonan K,Lambeth C,Kar K,Anderson JF,de Silva AM,Diamond MS,Koski RA,Marasco WA,Fikrig E

    更新日期:2005-12-01 00:00:00

  • Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus.

    abstract::We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chiocca S,Baker A,Cotten M

    更新日期:1997-04-01 00:00:00

  • DNA sequence alterations responsible for the synthesis of thermosensitive VP1 in temperature-sensitive BC mutants of simian virus 40.

    abstract::The segment of simian virus 40 (SV40) genome which is recognized as the BC domain encodes for the COOH-terminal end of the SV40 major capsid protein VP1. Mutations in this domain lead to the synthesis of a thermosensitive VP1 which fails to assemble mature SV40 at the nonpermissive temperature. We determined the DNA s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ng SC,Behm M,Bina M

    更新日期:1985-05-01 00:00:00

  • Capped nonviral sequences at the 5' end of the mRNAs of rice hoja blanca virus RNA4.

    abstract::Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ramirez BC,Garcin D,Calvert LA,Kolakofsky D,Haenni AL

    更新日期:1995-03-01 00:00:00