Human allograft limbal transplantation for corneal surface reconstruction.


:Limbal allograft transplantation was performed consecutively in 16 eyes with thermal or chemical burns (n = 5), Terrien's degeneration (n = 2), congenital sclerocornea (n = 1), Stevens-Johnson syndrome (n = 1), and chronic keratoconjunctivitis (n = 7), by transplanting randomly selected cadaver limbocorneal grafts to the recipient eye that had received superficial lamellar keratectomy to remove fibrovascular pannus. Oral cyclosporine A was administered immediately for 2.9 +/- 1.3 months. During 18.5 +/- 5.4 months of follow-up, the results showed improved visual acuity in 13 eyes (81.3%) and rapid (within 1 week) surface healing in 10 eyes (62.5%). Donor limbal tissue developed engorged and tortuous blood vessels in 12 eyes within 1-2 months, but these regressed within 3 months after surgery. No acute graft failure or allograft rejection could be identified. Twelve eyes (75%) showed total regression of vascularization and four eyes had decreased vascularization. These preliminary results indicate that limbal allograft transplantation may be able to reconstruct a corneal surface that has undergone bilateral diffuse destruction, including the loss of limbal epithelial stem cells.






Tsai RJ,Tseng SC




Has Abstract


1994-09-01 00:00:00














  • Toxicity and pharmacokinetics of subconjunctival amphotericin B. An experimental study.

    abstract::The pharmacokinetics and toxicity of subconjunctival (S/C) amphotericin B (AmB) were evaluated in Dutch-belted rabbits. Following the S/C injection of 1,500 micrograms of AmB, corneal and aqueous levels were determined by bioassay. The highest levels were present in the periphery of debrided corneas at 1 h (90.12 +/- ...


    pub_type: 杂志文章


    authors: O'Day DM,Smith R,Stevens JB,Williams TE,Robinson RD,Head WS

    更新日期:1991-09-01 00:00:00

  • The relation of conjunctival and corneal findings in severe ocular allergies.

    abstract:OBJECTIVE:Corneal lesions such as ulcers and erosions are major complications observed in patients with severe ocular allergic diseases. We aimed to look into the relationship between specific clinical conjunctival findings and the severity of corneal complications. METHODS:Thirty eyes of 6 patients with vernal kerato...


    pub_type: 杂志文章


    authors: Tanaka M,Dogru M,Takano Y,Miyake-Kashima M,Asano-Kato N,Fukagawa K,Tsubota K,Fujishima H

    更新日期:2004-07-01 00:00:00

  • Anatomical Changes in the Anterior Chamber Volume After Descemet Membrane Endothelial Keratoplasty.

    abstract:PURPOSE:To evaluate changes in the anterior chamber of eyes that have undergone Descemet membrane endothelial keratoplasty (DMEK) and to identify factors that affect these changes. METHODS:This retrospective study included 25 pseudophakic eyes of 25 patients who underwent DMEK. We determined the preoperative and posto...


    pub_type: 杂志文章


    authors: Onouchi H,Hayashi T,Shimizu T,Matsuzawa A,Suzuki Y,Kato N

    更新日期:2020-09-29 00:00:00

  • Glucocorticoids suppress corneal lymphangiogenesis.

    abstract:PURPOSE:To determine whether glucocorticoids suppress corneal lymphatic vessel growth (lymphangiogenesis) or induce lymphatic vessel regression. METHODS:We measured human lymphatic endothelial cell proliferation and collagen-induced tubulogenesis in culture conditions with and without dexamethasone, a potent glucocort...


    pub_type: 杂志文章


    authors: Steele MM,Kelley PM,Schieler AM,Tempero RM

    更新日期:2011-12-01 00:00:00

  • Corneal Transplantation in Auckland, New Zealand, 1999-2009: Indications, Patient Characteristics, Ethnicity, Social Deprivation, and Access to Services.

    abstract:PURPOSE:To analyze characteristics and indications for corneal transplantation in patients undergoing penetrating, lamellar, and endothelial keratoplasty in Auckland, New Zealand (NZ). METHODS:Corneal transplantation data from the NZ National Eye Bank and hospital records of corneal transplant recipients in the Auckla...


    pub_type: 杂志文章


    authors: Crawford AZ,McKelvie J,Craig JP,McGhee CN,Patel DV

    更新日期:2017-05-01 00:00:00

  • Ofloxacin in human serum, urine, and tear film after topical application.

    abstract::We evaluated the systemic absorption of ofloxacin eyedrops in humans and their availability in the tear film. Serum, urine, and tear film concentrations of ofloxacin were measured in 30 healthy women topically treated with 0.3% ofloxacin, in both eyes, four times daily for 10 1/2 days. Serum was collected before the f...


    pub_type: 杂志文章


    authors: Borrmann L,Tang-Liu DD,Kann J,Nista J,Lin ET,Frank J

    更新日期:1992-05-01 00:00:00

  • Combined Excimer Laser Photoablation and Amniotic Membrane Overlay for Relief of Symptomatic Discomfort in Gelatinous Drop-like Corneal Dystrophy.

    abstract:PURPOSE:To describe the efficacy of combined excimer laser photoablation and amniotic overlay membrane in the relief of symptomatic discomfort in a 17-year-old patient who had gelatinous drop-like corneal dystrophy. METHODS:The best-corrected visual acuity (BCVA) was measured with Snellen letters. Slit-lamp examinatio...


    pub_type: 杂志文章


    authors: Alex AF,Eter N,Uhlig CE

    更新日期:2015-10-01 00:00:00

  • Diabetes and corneal cell densities in humans by in vivo confocal microscopy.

    abstract:PURPOSE:Diabetes is accompanied by an increased autofluorescence of the cornea, probably because of accumulation of advanced glycation end products (AGEs). The pathogenic mechanism is still unknown. This study aimed to quantify differences in corneal cell densities between diabetic patients and healthy controls. METHO...


    pub_type: 杂志文章


    authors: Quadrado MJ,Popper M,Morgado AM,Murta JN,Van Best JA

    更新日期:2006-08-01 00:00:00

  • Eyelid Tattooing Induces Meibomian Gland Loss and Tear Film Instability.

    abstract:PURPOSE:To investigate the changes of meibomian gland (MG) and tear film stability in subjects with eyelid tattoos. METHODS:Forty female subjects were recruited. Ten subjects had eyelid tattoos and 30 subjects did not (control group). Tear film break-up time (TBUT) measurement and fluorescein staining were performed t...


    pub_type: 杂志文章


    authors: Lee YB,Kim JJ,Hyon JY,Wee WR,Shin YJ

    更新日期:2015-07-01 00:00:00

  • Recovery time of an optimally secreting meibomian gland.

    abstract:PURPOSE:To investigate the times for an optimally secreting meibomian gland (OSMG) to be drained of available liquid secretion, to recover with expressible liquid secretion, and then to be redrained. METHODS:Twelve subjects with no dry eye symptoms and mean age 21.3 +/- 1.9 years were recruited. Lipid layer thickness ...


    pub_type: 杂志文章


    authors: Blackie CA,Korb DR

    更新日期:2009-04-01 00:00:00

  • Trends in contact lens-related corneal ulcers at a tertiary referral center.

    abstract:PURPOSE:To evaluate the changes and trends in the number and characteristics of contact lens-related ulcers (CLRUs) and to compare the results with those of previously published series at our institution. METHODS:Medical records of all patients diagnosed with presumed bacterial corneal ulcers seen at the Cornea Servic...


    pub_type: 杂志文章


    authors: Yildiz EH,Airiani S,Hammersmith KM,Rapuano CJ,Laibson PR,Virdi AS,Hongyok T,Cohen EJ

    更新日期:2012-10-01 00:00:00

  • Experimental transplantation of corneal epithelium-like cells induced by Pax6 gene transfection of mouse embryonic stem cells.

    abstract:PURPOSE:Corneal epithelial stem cells are deficient in cases of limbal disorders, leading to conjunctival epithelial ingrowth, vascularization, and eventually visual disturbance. We introduced the eye development-associated transcription factor pax6 to embryonic stem (ES) cells and tested whether pax6-transfected cells...


    pub_type: 杂志文章


    authors: Ueno H,Kurokawa MS,Kayama M,Homma R,Kumagai Y,Masuda C,Takada E,Tsubota K,Ueno S,Suzuki N

    更新日期:2007-12-01 00:00:00

  • Mitomycin C does not inhibit exacerbation of granular corneal dystrophy type II induced by refractive surface ablation.

    abstract:PURPOSE:To evaluate the effect of mitomycin C (MMC) on the exacerbation of corneal opacity that occurs in patients with granular corneal dystrophy type II (GCD II) after refractive corneal surface ablation. METHODS:Ten eyes of patients with GCD II who underwent refractive corneal surface ablation with MMC were compare...


    pub_type: 杂志文章


    authors: Ha BJ,Kim TI,Choi SI,Stulting RD,Lee DH,Cho HS,Kim EK

    更新日期:2010-05-01 00:00:00

  • Intracorneal hemorrhage secondary to aphakic contact lens wear.

    abstract::Two cases of intracorneal hemorrhage developing as a complication of aphakic contact lens use are reported. Patient 1 had a penetrating keratoplasty to restore vision and therefore the histologic examination could be performed on the corneal button. In patient 2 the contact lens use was discontinued with partial resol...


    pub_type: 杂志文章


    authors: al-Hussaini AK,Friedlander MH,Karcioglu ZA

    更新日期:1992-01-01 00:00:00

  • Effectiveness of ciprofloxacin and ofloxacin in a prophylaxis model of Staphylococcus keratitis.

    abstract:PURPOSE:To determine the effectiveness of prophylactic fluoroquinolone treatment against staphylococci in a rabbit keratitis model. METHODS:Prophylactic ciprofloxacin or ofloxacin was applied as one topical drop 15 minutes before infection or as one drop at three time points (19, 17, and 15 minutes) before infection. ...


    pub_type: 杂志文章


    authors: Dajcs JJ,Moreau JM,Thibodeaux BA,Traidej M,Austin MS,Marquart ME,Stroman DW,O'Callaghan RJ

    更新日期:2001-11-01 00:00:00

  • Histological analysis of a cornea following experimental femtosecond laser ablation.

    abstract::Corneal photorefractive surgery is currently performed by ablation of corneal stroma under the stromal flap. A stromal flap is created using a femtosecond (FS) laser or mechanical microkeratome, although the FS laser procedure is considered safer and more accurate. This review assesses and compares the use of FS laser...


    pub_type: 杂志文章,评审


    authors: Sumioka T,Miyamoto T,Takatsuki R,Okada Y,Yamanaka O,Saika S

    更新日期:2014-11-01 00:00:00

  • Treatment of Acanthamoeba keratitis by corneal cross-linking.

    abstract:PURPOSE:To evaluate the efficacy of corneal cross-linking (CXL; riboflavin/ultraviolet A) as a simple therapy for Acanthamoeba keratitis. METHODS:Twenty rabbits were systemically anesthetized and the stroma of their right corneas was inoculated with a suspension of Acanthamoeba. Rabbits were divided into 2 groups: one...


    pub_type: 杂志文章


    authors: Berra M,Galperín G,Boscaro G,Zarate J,Tau J,Chiaradia P,Berra A

    更新日期:2013-02-01 00:00:00

  • Endothelial keratoplasty: the influence of insertion techniques and incision size on donor endothelial survival.

    abstract:PURPOSE:To determine the acute endothelial cell damage from trephination and tissue insertion in endothelial keratoplasty (EK) surgery. The influence of insertion technique (forceps insertion vs "pull-through" insertion) of donor tissue and incision size (3 vs 5 mm length) was assessed. METHODS:Forty precut 8.-mm-diam...


    pub_type: 杂志文章


    authors: Terry MA,Saad HA,Shamie N,Chen ES,Phillips PM,Friend DJ,Holiman JD,Stoeger C

    更新日期:2009-01-01 00:00:00

  • Assessment of keratocyte density in patients with keratoconus not using contact lenses.

    abstract:PURPOSE:To assess the keratocyte density (KD) in the cornea of patients with keratoconus not using contact lenses. METHOD:In this pilot study, the eyes (n = 68) of 35 patients with the diagnosis of keratoconus, who did not use contact lenses and the eyes(n = 70) of 35 healthy controls were prospectively examined using...


    pub_type: 杂志文章


    authors: Timucin OB,Karadag MF,Cinal A

    更新日期:2011-05-01 00:00:00

  • Evaluation of Meibomian Glands in Obstructive Sleep Apnea-Hypopnea Syndrome.

    abstract:PURPOSE:To evaluate the effect of obstructive sleep apnea-hypopnea syndrome (OSAHS) on the meibomian glands, ocular surface, and tear parameters. METHODS:The study included 59 individuals (32 subjects with OSAHS, 27 control subjects) who underwent polysomnography in the Chest Diseases Clinic. The right eyes of all ind...


    pub_type: 杂志文章


    authors: Muhafiz E,Ölçen M,Erten R,Bozkurt E

    更新日期:2020-06-01 00:00:00

  • Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas.

    abstract::After n-heptanol removal of the total corneal epithelium and a limbal lamellar keratectomy, 23 rabbit eyes developed features of limbal stem cell deficiency including conjunctival epithelial ingrowth, vascularization and chronic inflammation. One month later, 10 control eyes received a total keratectomy, and 13 experi...


    pub_type: 杂志文章


    authors: Kim JC,Tseng SC

    更新日期:1995-09-01 00:00:00

  • Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses.

    abstract:PURPOSE:To evaluate adhesion of Acanthamoeba trophozoites to different silicone hydrogel contact lens (SHCL) generations with and without multipurpose contact lens care solution (MPS) treatment. METHODS:Acanthamoeba lugdunensis L3a trophozoites were inoculated onto discs trimmed from SHCLs: first generation, Air Optix...


    pub_type: 杂志文章


    authors: Lee GH,Lee JE,Park MK,Yu HS

    更新日期:2016-05-01 00:00:00

  • Adenovirus epithelial keratitis.

    abstract::The authors sought to determine whether adenovirus could infect human corneal epithelium in vivo. They reviewed the medical records of six patients with adenovirus-positive viral corneal cultures who were examined at the Bascom Palmer Eye Institute between March 21, 1986, and December 31, 1992. The six patients with a...


    pub_type: 杂志文章


    authors: Chodosh J,Miller D,Stroop WG,Pflugfelder SC

    更新日期:1995-03-01 00:00:00

  • Expression of phosphorylated heat shock protein 27 during corneal epithelial wound healing.

    abstract:PURPOSE:The 27-kDa heat shock protein (HSP27) has been implicated in wound healing in multiple tissues. We investigated the expression and localization of phosphorylated HSP27 during epithelial wound healing in the murine cornea. METHODS:Corneas of 8- to 10-week-old C57BL6 mice were wounded by epithelial debridement (...


    pub_type: 杂志文章


    authors: Jain S,De la Cruz J,Kang E,Kojima T,Chang JH,Kim JY

    更新日期:2012-07-01 00:00:00

  • A stepwise approach to donor preparation and insertion increases safety and outcome of Descemet membrane endothelial keratoplasty.

    abstract:PURPOSE:Lamellar techniques for selective replacement of diseased corneal structures have recently been improved. Descemet membrane endothelial keratoplasty (DMEK) allows the sole replacement of the endothelium--Descemet membrane layer (EDM). However, wide-spread use of DMEK is currently limited because of problems wit...


    pub_type: 杂志文章


    authors: Kruse FE,Laaser K,Cursiefen C,Heindl LM,Schlötzer-Schrehardt U,Riss S,Bachmann BO

    更新日期:2011-05-01 00:00:00

  • Ocular Capnocytophaga infection in an edentulous, immunocompetent host.

    abstract::Severe microbial keratitis developed in a 74-year-old women receiving long-term corticosteroids. Corneal smears revealed slender, fusiform gram-negative bacilli, but the lesion continued to worsen despite intensive antibiotic treatment. After several days, Capnocytophaga sputigena was identified on culture. Specific a...


    pub_type: 杂志文章


    authors: Ormerod LD,Foster CS,Paton BG,Haaf J,Baker AS

    更新日期:1988-01-01 00:00:00

  • Scheimpflug Corneal Densitometry Changes After Trabeculectomy.

    abstract:PURPOSE:To study the possible changes in Scheimpflug corneal densitometry 6 months after mitomycin C-augmented trabeculectomy and to compare these measurements with healthy controls. METHODS:Corneal densitometry was monitored with the Pentacam HR3 before and 6 months after first-time uncomplicated mitomycin C-augmente...


    pub_type: 杂志文章


    authors: Pillunat KR,Orphal J,Haase M,Pillunat LE,Herber R

    更新日期:2020-07-27 00:00:00

  • Wound healing after keratorefractive surgery: review of biological and optical considerations.

    abstract::The introduction of the excimer laser for keratorefractive surgery in the 1990 s permanently reshaped the treatment landscape for correcting refractive errors, such as myopia, hyperopia, and astigmatism. Until that point, these treatments had relied on less predictable techniques, such as radial keratotomy and automat...


    pub_type: 杂志文章,评审


    authors: Azar DT,Chang JH,Han KY

    更新日期:2012-11-01 00:00:00

  • Suppression of corneal neovascularization by culture supernatant of human amniotic cells.

    abstract:PURPOSE:To examine the applicability of culture supernatant of human amniotic cells on basic fibroblast growth factor (bFGF)-induced corneal neovascularization. METHODS:Human amniotic epithelial and mesenchymal cells (AC) were obtained from human amniotic membranes by digesting with collagenase and maintained in serum...


    pub_type: 杂志文章


    authors: Kobayashi N,Kabuyama Y,Sasaki S,Kato K,Homma Y

    更新日期:2002-01-01 00:00:00

  • The castroviejo square graft: wound healing after 51 years.

    abstract:PURPOSE:To describe the histopathologic characteristics of a 51-year-old Castroviejo square graft that remained functional for more than 50 years and to describe the wound-healing characteristics over this period of time. METHODS:An 80-year-old woman with a history of keratoconus underwent penetrating keratoplasty wit...


    pub_type: 杂志文章


    authors: Graue-Hernández EO,Harocopos G,Bradley JC,Smith ME,Mannis MJ

    更新日期:2009-04-01 00:00:00