The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors.


:The monoclonal antibody STRO-1 identifies clonogenic bone marrow stromal cell progenitors (fibroblast colony-forming units [CFU-F]) in adult human bone marrow. These STRO-1+ CFU-F have previously been shown to give rise to cells with the phenotype of fibroblasts, adipocytes, and smooth muscle cells. In this study, the osteogenic potential of CFU-F derived from the STRO-1+ fraction of adult human bone marrow was determined. CFU-F were isolated from normal bone marrow aspirates by fluorescence activated cell sorting, based on their expression of the STRO-1 antigen. Osteogenic differentiation was assessed by the induction of alkaline phosphatase expression, by the formation of a mineralized matrix (hydroxyapatite), and by the production of the bone-specific protein osteocalcin. STRO-1+ cells were cultured in the presence of dexamethasone (DEX; 10(-8) mol/L), ascorbic acid 2-phosphate (ASC-2P; 100 mumol/L), and inorganic phosphate (PO4i; 2.9 mmol/L). After 2 weeks of culture, greater than 90% of the cells in each CFU-F colony stained positive for alkaline phosphatase using a monoclonal antibody specific for bone and liver alkaline phosphatase. Alkaline phosphatase activity was confirmed by histochemistry. A mineralized matrix developed in the CFU-F cultures, after 4 weeks of culture in the presence of DEX, ASC-2P, and PO4i. Mineralization was confirmed by both light and electron microscopy. The mineral was identified as hydroxyapatite by electron dispersive x-ray microanalysis and by x-ray diffraction analysis. In replicate cultures, osteocalcin release was shown after exposure of the cells to 1,25-dihydroxyvitamin D3 (10(-7) mol/L) both by radioimmunoassay and Northern blot analysis. This work provides direct evidence that adult human bone marrow-derived CFU-F are capable of differentiating into functional osteoblasts and that osteoprogenitors are present in the STRO-1+ population.






Gronthos S,Graves SE,Ohta S,Simmons PJ


Has Abstract


1994-12-15 00:00:00














  • Bim regulates the survival and suppressive capability of CD8+ FOXP3+ regulatory T cells during murine GVHD.

    abstract::CD8+ Foxp3+ T cells (Tregs) are a potent regulatory population whose functional and ontological similarities to CD4+ Fox3+ T cells have not been well delineated. Using an experimental model of graft-versus-host disease (GVHD), we observed that CD8+ Tregs were significantly less potent than CD4+ Tregs for the suppressi...


    pub_type: 杂志文章


    authors: Agle K,Vincent BG,Piper C,Belle L,Zhou V,Shlomchik W,Serody JS,Drobyski WR

    更新日期:2018-07-26 00:00:00

  • Intrauterine transplantation of human fetal mesenchymal stem cells from first-trimester blood repairs bone and reduces fractures in osteogenesis imperfecta mice.

    abstract::The inherited skeletal dysplasia osteogenesis imperfecta (OI) results in multiple fractures and is currently treated empirically. We transplanted human first-trimester fetal blood mesenchymal stem cells (MSCs) into homozygous oim mice in utero. This resulted in a two-thirds reduction in long bone fractures (P < .01), ...


    pub_type: 杂志文章


    authors: Guillot PV,Abass O,Bassett JH,Shefelbine SJ,Bou-Gharios G,Chan J,Kurata H,Williams GR,Polak J,Fisk NM

    更新日期:2008-02-01 00:00:00

  • Graft failure following bone marrow transplantation for severe aplastic anemia: risk factors and treatment results.

    abstract::Graft failure was analyzed in 625 patients receiving allogeneic bone marrow transplants from HLA-identical sibling donors as treatment for severe aplastic anemia. Sixty-eight (11%) had no or only transient engraftment. Second bone marrow transplants were successful in achieving extended survival in 16 of 27 patients w...


    pub_type: 杂志文章


    authors: Champlin RE,Horowitz MM,van Bekkum DW,Camitta BM,Elfenbein GE,Gale RP,Gluckman E,Good RA,Rimm AA,Rozman C

    更新日期:1989-02-01 00:00:00

  • New transgenic evidence for a system of sympathetic axons able to express tissue plasminogen activator (t-PA) within arterial/arteriolar walls.

    abstract::Sympathetic axons embedded in a few arterioles and vasa vasora were recently shown to store tissue plasminogen activator (t-PA) in vesicles. But the extension of such t-PA axons to arteries and arterioles throughout the organism has not been verified. Confirmation of this anatomy would identify a second significant so...


    pub_type: 杂志文章


    authors: Hao Z,Guo C,Jiang X,Krueger S,Pietri T,Dufour S,Cone RE,O'Rourke J

    更新日期:2006-07-01 00:00:00

  • Ethical considerations in genomic testing for hematologic disorders.

    abstract::As our technological capacities improve, genomic testing is increasingly integrating into patient care. The field of clinical hematology is no exception. Genomic testing carries great promise, but several ethical issues must be considered whenever such testing is performed. This review addresses these ethical consider...


    pub_type: 杂志文章,评审


    authors: Marron JM,Joffe S

    更新日期:2017-07-27 00:00:00

  • Transgenic overexpression of human IL-17E results in eosinophilia, B-lymphocyte hyperplasia, and altered antibody production.

    abstract::We have identified and cloned a novel human cytokine with homology to cytokines of the interleukin-17 (IL-17) family, which we have termed human IL-17E (hIL-17E). With the identification of several IL-17 family members, it is critical to understand the in vivo function of these molecules. We have generated transgenic ...


    pub_type: 杂志文章


    authors: Kim MR,Manoukian R,Yeh R,Silbiger SM,Danilenko DM,Scully S,Sun J,DeRose ML,Stolina M,Chang D,Van GY,Clarkin K,Nguyen HQ,Yu YB,Jing S,Senaldi G,Elliott G,Medlock ES

    更新日期:2002-10-01 00:00:00

  • In vivo administration of vascular endothelial growth factor (VEGF) and its antagonist, soluble neuropilin-1, predicts a role of VEGF in the progression of acute myeloid leukemia in vivo.

    abstract::Recent findings implied that the progression of hematologic malignancies, like that of solid tumors, is dependent on neovascularization. Recent studies on patients with acute myeloid leukemia (AML) showed increased levels of leukocyte-associated vascular endothelial growth factor (VEGF) and neovascularization of the b...


    pub_type: 杂志文章


    authors: Schuch G,Machluf M,Bartsch G Jr,Nomi M,Richard H,Atala A,Soker S

    更新日期:2002-12-15 00:00:00

  • Hepatitis viruses and non-Hodgkin lymphoma: epidemiology, mechanisms of tumorigenesis, and therapeutic opportunities.

    abstract::Over the past 2 decades considerable evidence has accumulated on the association between hepatitis C virus (HCV) and hepatitis B virus (HBV) and several hematologic malignancies, most notably B-cell non-Hodgkin lymphoma (NHL). In this review we summarize this evidence, address possible mechanisms whereby hepatitis vir...


    pub_type: 杂志文章,评审


    authors: Marcucci F,Mele A

    更新日期:2011-02-10 00:00:00

  • Divergent effects of interleukin-4 (IL-4) on the granulocyte colony-stimulating factor and IL-3-supported myeloid colony formation from normal and leukemic bone marrow cells.

    abstract::Human recombinant interleukin-4 (IL-4) was studied for its effects on myeloid progenitor cells from normal and leukemic bone marrow cells in the presence and absence of additional growth factors. IL-4 itself did not support myeloid cluster or colony formation (CFU-GM). However, cultures supplied with IL-4 (300 U/mL) a...


    pub_type: 杂志文章


    authors: Vellenga E,de Wolf JT,Beentjes JA,Esselink MT,Smit JW,Halie MR

    更新日期:1990-02-01 00:00:00

  • The role of apoptosis in the development of AGM hematopoietic stem cells revealed by Bcl-2 overexpression.

    abstract::Apoptosis is an essential process in embryonic tissue remodeling and adult tissue homeostasis. Within the adult hematopoietic system, it allows for tight regulation of hematopoietic cell subsets. Previously, it was shown that B-cell leukemia 2 (Bcl-2) overexpression in the adult increases the viability and activity of...


    pub_type: 杂志文章


    authors: Orelio C,Harvey KN,Miles C,Oostendorp RA,van der Horn K,Dzierzak E

    更新日期:2004-06-01 00:00:00

  • The inhibitory anti-FGFR3 antibody, PRO-001, is cytotoxic to t(4;14) multiple myeloma cells.

    abstract::The association of fibroblast growth factor receptor 3 (FGFR3) expression with t(4;14) multiple myeloma (MM) and the demonstration of the transforming potential of this receptor tyrosine kinase (RTK) make it a particularly attractive target for drug development. We report here a novel and highly specific anti-FGFR3-ne...


    pub_type: 杂志文章


    authors: Trudel S,Stewart AK,Rom E,Wei E,Li ZH,Kotzer S,Chumakov I,Singer Y,Chang H,Liang SB,Yayon A

    更新日期:2006-05-15 00:00:00

  • Physical and functional association of Fc alpha R with protein tyrosine kinase Lyn.

    abstract::In this report, we show that the Src family nonreceptor protein tyrosine kinase (PTK) Lyn associates with aggregated IgA Fc receptor (Fc alpha R) in the monocytic cell line THP-1. Receptor aggregation and subsequent immunoprecipitation of receptor complexes with huIgA adsorbed to nitrocellulose particles shows that Ly...


    pub_type: 杂志文章


    authors: Gulle H,Samstag A,Eibl MM,Wolf HM

    更新日期:1998-01-15 00:00:00

  • Mutations of the PML tumor suppressor gene in acute promyelocytic leukemia.

    abstract::The promyelocytic leukemia (PML) tumor suppressor of acute promyelocytic leukemia (APL) is essential for a number of proapoptotic and growth-suppressive pathways as well as for the activity of differentiating agents such as retinoic acid (RA). In human APL, the dose of PML is reduced to heterozygosity given that one a...


    pub_type: 杂志文章


    authors: Gurrieri C,Nafa K,Merghoub T,Bernardi R,Capodieci P,Biondi A,Nimer S,Douer D,Cordon-Cardo C,Gallagher R,Pandolfi PP

    更新日期:2004-03-15 00:00:00

  • Self-renewal in late-stage erythropoiesis.

    abstract::Extensive in vitro self-renewal of proerythroblasts from the earliest period of definitive erythropoiesis, as reported by England and co-investigators in this issue of Blood, expands the potential role of self-renewal in mammalian hematopoiesis and suggests a possible source of blood cells for clinical treatments. ...


    pub_type: 评论,杂志文章


    authors: Koury MJ

    更新日期:2011-03-03 00:00:00

  • alpha-thalassemia resulting from a negative chromosomal position effect.

    abstract::To date, all of the chromosomal deletions that cause alpha-thalassemia remove the structural alpha genes and/or their regulatory element (HS -40). A unique deletion occurs in a single family that juxtaposes a region that normally lies approximately 18-kilobase downstream of the human alpha cluster, next to a structura...


    pub_type: 杂志文章


    authors: Barbour VM,Tufarelli C,Sharpe JA,Smith ZE,Ayyub H,Heinlein CA,Sloane-Stanley J,Indrak K,Wood WG,Higgs DR

    更新日期:2000-08-01 00:00:00

  • Factor VIII gene (F8) mutation and risk of inhibitor development in nonsevere hemophilia A.

    abstract::Neutralizing antibodies (inhibitors) toward factor VIII form a severe complication in nonsevere hemophilia A, profoundly aggravating the bleeding pattern. Identification of high-risk patients is hampered by lack of data that take exposure days to therapeutic factor VIII concentrates into account. In the INSIGHT study,...


    pub_type: 杂志文章,多中心研究


    authors: Eckhardt CL,van Velzen AS,Peters M,Astermark J,Brons PP,Castaman G,Cnossen MH,Dors N,Escuriola-Ettingshausen C,Hamulyak K,Hart DP,Hay CR,Haya S,van Heerde WL,Hermans C,Holmström M,Jimenez-Yuste V,Keenan RD,Klamroth R

    更新日期:2013-09-12 00:00:00

  • T lymphocytes from invaded lymph nodes in patients with B-cell-derived non-Hodgkin's lymphoma: reactivity toward the malignant clone.

    abstract::Tumor-infiltrating T lymphocytes (TIL-T) are always present in B-cell-derived non-Hodgkin's lymphoma (NHL). In this investigation, we explored the possibility that collaboration might exist between these cells. TIL-T were isolated from 39 lymph nodes of patients with NHL. In most of the cases, few of them (less than 1...


    pub_type: 杂志文章


    authors: Jacob MC,Piccinni MP,Bonnefoix T,Sotto MF,Couderc P,Bensa JC,Sotto JJ

    更新日期:1990-03-01 00:00:00

  • Expression of oncogenic K-ras from its endogenous promoter leads to a partial block of erythroid differentiation and hyperactivation of cytokine-dependent signaling pathways.

    abstract::When overexpressed in primary erythroid progenitors, oncogenic Ras leads to the constitutive activation of its downstream signaling pathways, severe block of terminal erythroid differentiation, and cytokine-independent growth of primary erythroid progenitors. However, whether high-level expression of oncogenic Ras is ...


    pub_type: 杂志文章


    authors: Zhang J,Liu Y,Beard C,Tuveson DA,Jaenisch R,Jacks TE,Lodish HF

    更新日期:2007-06-15 00:00:00

  • Prevalence and predictors of chronic health conditions after hematopoietic cell transplantation: a report from the Bone Marrow Transplant Survivor Study.

    abstract::Long-term survival is now an expected outcome after hematopoietic cell transplantation (HCT). However, the burden of morbidity long-term after HCT remains unknown. We examined the magnitude of risk of chronic health conditions reported by 1022 HCT survivors and their siblings (n = 309). A severity score (grades 1 [mil...


    pub_type: 杂志文章


    authors: Sun CL,Francisco L,Kawashima T,Leisenring W,Robison LL,Baker KS,Weisdorf DJ,Forman SJ,Bhatia S

    更新日期:2010-10-28 00:00:00

  • AC133, a novel marker for human hematopoietic stem and progenitor cells.

    abstract::AC133 is one of a new panel of murine hybridoma lines producing monoclonal IgG antibodies (mAbs) to a novel stem cell glycoprotein antigen with a molecular weight of 120 kD. AC133 antigen is selectively expressed on CD34(bright) hematopoietic stem and progenitor cells (progenitors) derived from human fetal liver and b...


    pub_type: 杂志文章


    authors: Yin AH,Miraglia S,Zanjani ED,Almeida-Porada G,Ogawa M,Leary AG,Olweus J,Kearney J,Buck DW

    更新日期:1997-12-15 00:00:00

  • Perturbed hematopoiesis in mice lacking ATMIN.

    abstract::The ataxia telangiectasia mutated (ATM)-interacting protein ATMIN mediates noncanonical ATM signaling in response to oxidative and replicative stress conditions. Like ATM, ATMIN can function as a tumor suppressor in the hematopoietic system: deletion of Atmin under the control of CD19-Cre results in B-cell lymphomas i...


    pub_type: 杂志文章


    authors: Anjos-Afonso F,Loizou JI,Bradburn A,Kanu N,Purewal S,Da Costa C,Bonnet D,Behrens A

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  • Dasatinib crosses the blood-brain barrier and is an efficient therapy for central nervous system Philadelphia chromosome-positive leukemia.

    abstract::Although imatinib, a BCR-ABL tyrosine kinase inhibitor, is used to treat acute Philadelphia chromosome-positive (Ph(+)) leukemia, it does not prevent central nervous system (CNS) relapses resulting from poor drug penetration through the blood-brain barrier. Imatinib and dasa-tinib (a dual-specific SRC/BCR-ABL kinase i...


    pub_type: 临床试验,杂志文章


    authors: Porkka K,Koskenvesa P,Lundán T,Rimpiläinen J,Mustjoki S,Smykla R,Wild R,Luo R,Arnan M,Brethon B,Eccersley L,Hjorth-Hansen H,Höglund M,Klamova H,Knutsen H,Parikh S,Raffoux E,Gruber F,Brito-Babapulle F,Dombret H,Dua

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  • Perforin and interferon-gamma activities independently control tumor initiation, growth, and metastasis.

    abstract::Perforin (pfp) and interferon-gamma (IFN-gamma) together in C57BL/6 (B6) and BALB/c mouse strains provided optimal protection in 3 separate tumor models controlled by innate immunity. Using experimental (B6, RM-1 prostate carcinoma) and spontaneous (BALB/c, DA3 mammary carcinoma) models of metastatic cancer, mice defi...


    pub_type: 杂志文章


    authors: Street SE,Cretney E,Smyth MJ

    更新日期:2001-01-01 00:00:00

  • Isolated follicular lymphoma cells are resistant to apoptosis and can be grown in vitro in the CD40/stromal cell system.

    abstract::Low-grade follicular non-Hodgkin's lymphomas are characterized by the presence of a t(14;18) chromosomal translocation that results in deregulation of the B-cell lymphoma (Bcl-2) gene. Studies in cell lines and transgenic animal models have suggested that this results in the suppression of apoptotic cell death in germ...


    pub_type: 杂志文章


    authors: Johnson PW,Watt SM,Betts DR,Davies D,Jordan S,Norton AJ,Lister TA

    更新日期:1993-09-15 00:00:00

  • Immunoproteasome down-modulation enhances the ability of dendritic cells to stimulate antitumor immunity.

    abstract::The process of dendritic cell (DC) maturation, critical for effective DC-based immunotherapy, also alters the proteasome such that peptides presented in the context of HLA class I are generated not by the constitutive proteasome, but by the immunoproteasome. Cytotoxic T lymphocytes (CTLs) induced by such DCs might not...


    pub_type: 杂志文章


    authors: Dannull J,Lesher DT,Holzknecht R,Qi W,Hanna G,Seigler H,Tyler DS,Pruitt SK

    更新日期:2007-12-15 00:00:00

  • Critical roles for Rac GTPases in T-cell migration to and within lymph nodes.

    abstract::Naive T cells continuously recirculate between secondary lymphoid tissue via the blood and lymphatic systems, a process that maximizes the chances of an encounter between a T cell and its cognate antigen. This recirculation depends on signals from chemokine receptors, integrins, and the sphingosine-1-phosphate recepto...


    pub_type: 杂志文章


    authors: Faroudi M,Hons M,Zachacz A,Dumont C,Lyck R,Stein JV,Tybulewicz VL

    更新日期:2010-12-16 00:00:00

  • Defective anti-polysaccharide response and splenic marginal zone disorganization in ALPS patients.

    abstract::Autoimmune lymphoproliferative syndrome (ALPS) caused by impaired FAS-mediated apoptosis of lymphocytes is characterized by lymphoproliferation, autoimmunity, but also an increased risk of invasive bacterial infection, notably following splenectomy. We surveyed a cohort of 100 ALPS patients (including 33 splenectomize...


    pub_type: 杂志文章


    authors: Neven B,Bruneau J,Stolzenberg MC,Meyts I,Magerus-Chatinet A,Moens L,Lanzarotti N,Weller S,Amiranoff D,Florkin B,Bader-Meunier B,Leverger G,Ferster A,Chantrain C,Blanche S,Picard C,Molina TJ,Brousse N,Durandy A,Rizzi

    更新日期:2014-09-04 00:00:00

  • Large granular lymphocytes in human peripheral blood: ultrastructural and cytochemical characterization of the granules.

    abstract::Large granular lymphocytes (LGL) are defined as nonadherent mononuclear cells with cytoplasmic azurophilic granules, avid receptors for the Fc portion of IgG, and cytotoxic functions (NK or ADCC activities). In the present study, the granules of LGL isolated from human peripheral blood have been analyzed by enzyme cyt...


    pub_type: 杂志文章


    authors: Grossi CE,Cadoni A,Zicca A,Leprini A,Ferrarini M

    更新日期:1982-02-01 00:00:00

  • Activation of human platelets by the rabbit anticardiolipin antibodies.

    abstract::Affinity purified anticardiolipin antibodies (ACLA) raised in rabbits showed cross-reactivities with various negatively charged phospholipids as shown by both the solid phase enzyme-linked immunosorbent assay (ELISA) and inhibition studies. In ELISA, ACLA showed strong cross-reactivity to both sphingomyelin (SM) and p...


    pub_type: 杂志文章


    authors: Lin YL,Wang CT

    更新日期:1992-12-15 00:00:00

  • Cytokine regulation of the human burst-forming unit-megakaryocyte.

    abstract::The human burst-forming unit-megakaryocyte (BFU-MK) is a primitive megakaryocytic progenitor cell. A marrow cell population enriched for BFU-MK (CD34+ DR-) was obtained by monoclonal antibody labeling and fluorescence-activated cell sorting. CD34+DR- cells were assayed in a serum-depleted, fibrin clot culture system. ...


    pub_type: 杂志文章


    authors: Briddell RA,Hoffman R

    更新日期:1990-08-01 00:00:00