Migrating motor complex recorded spontaneously and induced by motilin and erythromycin in an ex vivo rabbit intestinal preparation.


:We investigated basal motility and the motor effects of motilin, erythromycin, and prostigmine on segments of rabbit gastrointestinal tract removed from extrinsic neural and vascular pathway and immersed in an oxygenated organ bath. Motility was recorded by means of four strain gauges sutured on the serosal surface of the segment. During basal recording, clusters of duodenal contractions that propagated distally, resembling phase III activity of migrating motor complex, were seen. Motilin (10(-6) M) and erythromycin (10(-6) M) induced a propagated cluster of contractions similar to the phase III recorded during the basal period. Prostigmine (10(-6) M) induced a simultaneous increase in gastric and small intestinal motility. Atropine (10(-5) M) prevented the motor effect of motilin, erythromycin, and prostigmine. Thus, MMCs do not appear to require central input for initiation and propagation. Motilin and erythromycin stimulate MMCs through an enteric cholinergic mechanism; therefore, the previously reported smooth muscle receptors for both substances were not apparent in the ex vivo preparation.






Marzio L,Grossi L,Martelli L,Falcucci M,Lapenna D




Has Abstract


1994-01-01 00:00:00
















  • Amiloride enhances atrial natriuretic factor stimulation of cGMP accumulation in rat glomeruli.

    abstract::The effects of amiloride on ANF binding and ANF stimulation of cGMP were evaluated in rat glomeruli. Amiloride increased ANF binding to whole glomeruli and to glomerular membrane preparations. In contrast, amiloride enhanced ANF-stimulated cGMP accumulation only at 37 degrees C in whole glomeruli, but not at 4 degrees...


    pub_type: 杂志文章


    authors: Iwata T,Hardee E,Frohlich ED,Cole FE

    更新日期:1989-05-01 00:00:00

  • Open conformation of adipokinetic hormone receptor from the malaria mosquito facilitates hormone binding.

    abstract::Insect flight requires rapid mobilization of energy reserves during flight, which is mediated and regulated by hormonal control via adipokinetic hormones. The structure of the G-protein receptors to which these hormones bind, are crucial in understanding many of the physiological processes in which they play a central...


    pub_type: 杂志文章


    authors: Mugumbate G,Jackson GE,van der Spoel D

    更新日期:2011-03-01 00:00:00

  • Analysis of conserved residues of the human puromycin-sensitive aminopeptidase.

    abstract::The puromycin-sensitive aminopeptidase (ApPS) is a zinc metallopeptidase involved in the degradation of neuropeptides. Putative catalytic residues of the enzyme, Cys146, Glu338, and Lys396 were mutated, and the resultant mutant enzymes characterized. ApPS C146S exhibited normal catalytic activity, ApPS E338A exhibited...


    pub_type: 杂志文章


    authors: Thompson MW,Hersh LB

    更新日期:2003-09-01 00:00:00

  • Cholecystokinin in plasma and cerebrospinal fluid--a study in healthy young women.

    abstract::Cholecystokinin (CCK) is widely distributed in the brain and is known to affect behavioral and physiological functions including anxiety and pain. The expression of CCK has been shown to be regulated by estrogen and to vary during the estrous cycle in rat brain. In the present study CCK was determined in plasma from 2...


    pub_type: 杂志文章


    authors: Lundberg K,Hilke S,Nordin C,Theodorsson E,Josefsson A

    更新日期:2010-08-01 00:00:00

  • Comparison of neurokinin SP with diazepam in effects on memory and fear parameters in the elevated T-maze free exploration paradigm.

    abstract::The elevated T-maze was combined with a free exploration protocol, which, in contrast to the conventional procedure, dispenses with handling of the animals during the experimental sessions. This allows measurement of fear indexes derived from the elevated plus-maze as well as assessment of acquisition of open arm avoi...


    pub_type: 杂志文章


    authors: Echeverry MB,Hasenöhrl RU,Huston JP,Tomaz C

    更新日期:2001-07-01 00:00:00

  • Anxiolytic effect of motilin and reversal with GM-109, a motilin antagonist, in mice.

    abstract::There have been few reports on the effects of the brain-gut peptide motilin on the central nervous system (CNS). We administered motilin intracerebroventricularly to mice and investigated the effect of motilin on anxiety using an elevated plus-maze. Motilin produced a significant decrease in anxiety with an inverted U...


    pub_type: 杂志文章


    authors: Momose K,Inui A,Asakawa A,Ueno N,Nakajima M,Kasuga M

    更新日期:1998-01-01 00:00:00

  • Synthesis and biological activity of adipokinetic hormone analogues modified at the C-terminus.

    abstract::A series of Locusta adipokinetic hormone I (AKH-I), < QLNFTPNWGTa, analogues, were synthesized with modifications at the C-terminal threonine residue using a combination of solid- and liquid-phase methodology and evaluated in Locusta migratoria, in a lipid mobilization assay in vivo and an acetate uptake assay in vitr...


    pub_type: 杂志文章


    authors: Lee MJ,Goldsworthy GJ,Poulos CP,Velentza A

    更新日期:1996-01-01 00:00:00

  • Elevated plasma levels of immunoreactive urotensin II and its increased urinary excretion in patients with Type 2 diabetes mellitus: association with progress of diabetic nephropathy.

    abstract::Urotensin II (UII) is the most potent vasoconstrictor peptide ever identified. In order to clarify the pathophysiological role of UII in diabetes mellitus, we examined plasma immunoreactive UII levels and urinary excretion of immunoreactive UII in 10 control subjects and 48 patients with Type 2 diabetes mellitus. The ...


    pub_type: 杂志文章


    authors: Totsune K,Takahashi K,Arihara Z,Sone M,Murakami O,Ito S,Kikuya M,Ohkubo T,Hashimoto J,Imai Y

    更新日期:2004-10-01 00:00:00

  • N-terminal 10- and 12-kDa POMC fragments stimulate differentiation of lactotrophs.

    abstract::Medium conditioned by a highly enriched population of gonadotrophs, cultured as reaggregates in the presence of 0.01 nM GnRH, was concentrated, separated on a reversed-phase HPLC column, and tested for activity on lactotroph development in pituitary reaggregate cell cultures of 14-day-old rats. The number of cells exp...


    pub_type: 杂志文章


    authors: Van Bael A,Vande Vijver V,Devreese B,Van Beeumen J,Denef C

    更新日期:1996-01-01 00:00:00

  • Des-acetyl MSH and gamma-MSH act as partial agonists to alpha-MSH on the Anolis melanophore.

    abstract::The biological activities of alpha-MSH des-acetyl MSH, gamma-MSH and LPH37-58 were compared using the Anolis rate method of bioassay. Dose-response data showed LPH37-58 to be equipotent with alpha-MSH, but des-acetyl MSH and gamma-MSH were found to be much less active. The effect of LPH37-58 was additive to that of al...


    pub_type: 杂志文章


    authors: McCormack AM,Carter RJ,Thody AJ,Shuster S

    更新日期:1982-01-01 00:00:00

  • The cationic tetradecapeptide mastoparan as a privileged structure for drug discovery: Enhanced antimicrobial properties of mitoparan analogues modified at position-14.

    abstract::Mastoparan (MP) peptides, distributed in insect venoms, induce a local inflammatory response post envenomation. Most endogenous MPs share common structural elements within a tetradecapeptide sequence that adopts an amphipathic helix whilst traversing biological membranes and when bound to an intracellular protein targ...


    pub_type: 杂志文章


    authors: Howl J,Howl L,Jones S

    更新日期:2018-03-01 00:00:00

  • Characterization of neurotensin(6-13) from an hepatic fibrolamellar carcinoma.

    abstract::Neurotensin(6-13) has been isolated and sequenced as the major form of neurotensin-like immunoreactivity (NTLI) in a human hepatic fibrolamellar carcinoma. Circulating NTLI in the patient, especially C-terminal, was very high. In additional studies, NT(6-13) was synthesized and compared with the purified tumor NTLI by...


    pub_type: 杂志文章


    authors: Read D,Shulkes A,Fernley R,Simpson R

    更新日期:1991-07-01 00:00:00

  • Processing of long-acting natriuretic peptide and vessel dilator in human plasma and serum.

    abstract::High performance-gel permeation chromatography (HP-GPC) evaluation of human plasma and serum incubated at 37 degrees C up to 24 h after the addition of long-acting natriuretic peptide (LANP) revealed a 25% decrease in LANP's peak at 2 h followed by an increase in plasma without EDTA at 8 h (p < 0.001). Vessel dilator'...


    pub_type: 杂志文章


    authors: Overton RM,Vesely DL

    更新日期:1996-01-01 00:00:00

  • Unexpected high density of neuropeptide Y Y1 receptors in the guinea pig spleen.

    abstract::Receptors for neuropeptide Y (NPY) and peptide YY (PYY) have been extensively characterized in the brain. Less is known about NPY receptor subtypes in the spleen, though it is well established that NPY produces vascular contraction in this tissue. In the present study, we found an unusually high density of Y1 receptor...


    pub_type: 杂志文章


    authors: Gehlert DR,Gackenheimer SL

    更新日期:1996-01-01 00:00:00

  • From blood to brain through BBB and astrocytic signaling.

    abstract::In this Festschrift, I discuss the career and guiding principles to which Abba J. Kastin has adhered during the last 20 years we worked together. I briefly describe the history of our joint laboratory group, the context of studies of peptide permeation across the blood-brain barrier (BBB), and newer developments in th...


    pub_type: 历史文章,杂志文章


    authors: Pan W

    更新日期:2015-10-01 00:00:00

  • Discovery of novel antimicrobial peptides with unusual cysteine motifs in dandelion Taraxacum officinale Wigg. flowers.

    abstract::Three novel antimicrobial peptides designated ToAMP1, ToAMP2 and ToAMP3 were purified from Taraxacum officinale flowers. Their amino acid sequences were determined. The peptides are cationic and cysteine-rich and consist of 38, 44 and 42 amino acid residues for ToAMP1, ToAMP2 and ToAMP3, respectively. Importantly, acc...


    pub_type: 杂志文章


    authors: Astafieva AA,Rogozhin EA,Odintsova TI,Khadeeva NV,Grishin EV,Egorov TsA

    更新日期:2012-08-01 00:00:00

  • The postnatal development of VIP binding sites in rat forebrain and hindbrain.

    abstract::The specific binding of 125I-labeled vasoactive intestinal peptide (VIP) to brain membranes from the forebrain and hindbrain regions of 2 to 37-day postnatal rats was measured. In both regions of the brain, VIP binding was low but detectable two days after birth and rose markedly between postnatal days 7 and 17. This ...


    pub_type: 杂志文章


    authors: Roth BL,Beinfeld MC

    更新日期:1985-01-01 00:00:00

  • Peripheral obestatin has no effect on feeding behavior and brain Fos expression in rodents.

    abstract::Obestatin is produced in the stomach from proghrelin by post-translational cleavage. The initial report claimed anorexigenic effects of obestatin in mice. Contrasting studies indicated no effect of obestatin on food intake (FI). We investigated influences of metabolic state (fed/fasted), environmental factors (dark/li...


    pub_type: 杂志文章


    authors: Kobelt P,Wisser AS,Stengel A,Goebel M,Bannert N,Gourcerol G,Inhoff T,Noetzel S,Wiedenmann B,Klapp BF,Taché Y,Mönnikes H

    更新日期:2008-06-01 00:00:00

  • Enzymatic cleavage of thymopoietin oligopeptides by pancreatic and intestinal brush-border enzymes.

    abstract::The intestinal enzymatic degradation of the immunomodulating peptides thymotrinan (TP3), thymocartin (TP4), and thymopentin (TP5), three oligopeptides derived from the naturally occurring thymus hormone thymopoietin, was investigated to evaluate their potential for peroral drug delivery. In the presence of brush-borde...


    pub_type: 杂志文章


    authors: Heizmann J,Langguth P,Biber A,Oschmann R,Merkle HP,Wolffram S

    更新日期:1996-01-01 00:00:00

  • Transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-A gene.

    abstract::Activation of natriuretic peptide receptor-A (NPRA) produces the second messenger cGMP, which plays a pivotal role in maintaining blood pressure and cardiovascular homeostasis. In the present study, we have examined the role of trans-acting factor Ets-1 in transcriptional regulation of Npr1 gene (coding for NPRA). Usi...


    pub_type: 杂志文章


    authors: Kumar P,Arise KK,Pandey KN

    更新日期:2006-07-01 00:00:00

  • Combined effects of vasoactive intestinal peptide and dopamine on adenylate cyclase in prolactin-secreting cells.

    abstract::VIP stimulates adenylate cyclase activity of male and female rat anterior pituitaries and human prolactinomas, while dopamine inhibits the enzyme activity of female rat pituitaries and prolactinomas. A dopamine inhibited cyclase can be detected also in male rats provided the enzyme activity is increased by VIP. The an...


    pub_type: 杂志文章


    authors: Oliva D,Vallar L,Giannattasio G,Spada A,Nicosia S

    更新日期:1984-11-01 00:00:00

  • Post-trial administration of vasopressin in humans does not enhance memory formation (vasopressin and memory consolidation).

    abstract::Many animal studies show an enhancing effect of vasopressin (VP) on memory, but not all human studies could confirm this finding. This study examined the influence of post-learning administration of VP (40 IU, intranasally) on the consolidation of declarative memories in healthy humans during different intervals of sl...


    pub_type: 临床试验,杂志文章


    authors: Gais S,Sommer M,Fischer S,Perras B,Born J

    更新日期:2002-03-01 00:00:00

  • Altered alertness of vasopressin-secreting transgenic mice.

    abstract::We evaluated behavior and cognitive performance in a line of transgenic mice that overexpress the rat gene for vasopressin. Open field testing revealed greatest habituation in homozygous mice. Passive avoidance performance indicated equal learning and memory ability of transgenic compared to normal mice. Drinking beha...


    pub_type: 杂志文章


    authors: Miller M,Haroutunian V,Wiltshire-Clement M

    更新日期:1995-01-01 00:00:00

  • Structural requirements for galanin interaction with receptors from pancreatic beta cells and from brain tissue of the rat.

    abstract::The binding activity of several galanin fragments and analogs was measured on specific receptors present in rat brain and the rat pancreatic beta cell line Rin m 5F. In both tissues it was observed that: 1) galanin(3-29), galanin(10-29) and [Ile2]-galanin were ineffective for inhibiting [125I] galanin binding and 2) a...


    pub_type: 杂志文章


    authors: Lagny-Pourmir I,Lorinet AM,Yanaihara N,Laburthe M

    更新日期:1989-07-01 00:00:00

  • Somatostatin analogs with affinity for opiate receptors in rat brain binding assay.

    abstract::The somatostatin analogs D-Phe-Cys-D-Trp-Lys-Thr-Cys-Thr and the corresponding penicillamine compounds have been prepared and tested for their ability to displace [3H]naloxone and [3H] [D-Ala2, D-Leu5]enkephalin from rat brain receptors. While somatostatin and the cystine containing peptide displayed little or no pref...


    pub_type: 杂志文章


    authors: Pelton JT,Gulya K,Hruby VJ,Duckles S,Yamamura HI

    更新日期:1985-01-01 00:00:00

  • The state versus amyloid-beta: the trial of the most wanted criminal in Alzheimer disease.

    abstract::Investigators studying the primary culprit responsible for Alzheimer disease have, for the past two decades, primarily focused on amyloid-beta (Abeta). Here, we put Abeta on trial and review evidence amassed by the prosecution that implicate Abeta and also consider arguments and evidence gathered by the defense team w...


    pub_type: 杂志文章,评审


    authors: Rottkamp CA,Atwood CS,Joseph JA,Nunomura A,Perry G,Smith MA

    更新日期:2002-07-01 00:00:00

  • Selective autonomic modulation by mu- and kappa-opioid receptors in the hindbrain.

    abstract::In the Nucleus Ambiguus (NA) mu-receptors increased mean arterial pressure (MAP) and heart rate (HR) and kappa-receptors decreased MAP without altering HR. Mu-receptors in the Dorsal Motor Nucleus of the Vagus (DMV) increased MAP without changing HR whereas kappa-receptors in the DMV decreased HR without changing MAP....


    pub_type: 杂志文章


    authors: Hassen AH,Broudy EP

    更新日期:1988-01-01 00:00:00

  • Receptors for GRP/bombesin-like peptides in the rat forebrain.

    abstract::Binding sites in the rat forebrain were characterized using (125I-Tyr4)bombesin as a receptor probe. Pharmacology experiments indicate that gastrin releasing peptide (GRP) and the GRP fragments GRP as well as Ac-GRP inhibited radiolabeled (Tyr4)bombesin binding with high affinity. Biochemistry experiments indicated th...


    pub_type: 杂志文章


    authors: Wolf SS,Moody TW

    更新日期:1985-01-01 00:00:00

  • Novel synthetic peptides from the C-terminal heparin binding domain of fibronectin with heparin binding activity.

    abstract::To identify a minimal peptide ligand that participates in recognition of heparin, we synthesized peptides extending a 29-kDa fragment in the C-terminal heparin binding domain of fibronectin. We obtained evidence that two peptides (designated D5 and D1) exhibit inhibitory activity on binding of the 29-kDa fragment to h...


    pub_type: 杂志文章


    authors: Mohri H,Katoh K,Motomura S,Okubo T

    更新日期:1996-01-01 00:00:00

  • Naturally-occurring TGR5 agonists modulating glucagon-like peptide-1 biosynthesis and secretion.

    abstract::Selective GLP-1 secretagogues represent a novel potential therapy for type 2 diabetes mellitus. This study examined the GLP-1 secretory activity of the ethnomedicinal plant, Fagonia cretica, which is postulated to possess anti-diabetic activity. After extraction and fractionation extracts and purified compounds were t...


    pub_type: 杂志文章


    authors: Jafri L,Saleem S,Calderwood D,Gillespie A,Mirza B,Green BD

    更新日期:2016-04-01 00:00:00