Abstract:
:Hep G2 cells, an established cell line derived from a human hepatoma, have retained a number of hepatocytic phase I and II reactions. The influence of picolines (2-, 3- and 4-methylpyridine), related compounds and some classical enzyme inducers on specific glutathione transferase (GST) activity and its subunit composition in Hep G2 cells was investigated. Increased GST activity was observed for rifamycin, phenobarbital, pyrazine and the picolines, of which the 4-isomer was the strongest inducer. The GST subunits were analysed by HPLC. GSTP1, GSTM1a, GSTA1 and GSTA2 were present in control Hep G2 cells. GSTM1a disappeared or was strongly reduced under the influence of the test chemicals. All GST increases were due to augmented GSTA1 expression. Thus, picolines stimulate GST activity in Hep G2 cells by influencing the class alpha GSTA1.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Dierickx PJdoi
10.1016/0006-2952(94)90597-5subject
Has Abstractpub_date
1994-11-16 00:00:00pages
1976-8issue
10eissn
0006-2952issn
1873-2968pii
0006-2952(94)90597-5journal_volume
48pub_type
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