Abstract:
:RNase MRP is a ribonucleoprotein endoribonuclease that has been shown to cleave mitochondrial primer RNA sequences from a variety of sources. Most of the RNase MRP activity is found in the nucleus where it plays a role in the processing of 5.8S rRNA. A temperature-conditional point mutation in the yeast RNA component of the enzyme has been identified. This mutation results in a loss of normal rRNA processing at the nonpermissive temperature while cellular levels of the RNA component of RNase MRP remain stable. High-copy suppressor analysis of this point mutation was employed to identify interacting proteins. A unique suppressor, termed SNM1 (suppressor of nuclear mitochondrial endoribonuclease 1), was identified repeatedly. The SNM1 gene was localized to the right arm of chromosome IV, directly adjacent to the SNF1 gene, and it contains an open reading frame encoding a protein of 198 amino acids. The protein contains a leucine zipper motif, a zinc-cluster motif, and a serine/lysine-rich tail. The gene was found to be essential for viability in a yeast cell, consistent with it being a protein component of the RNase MRP ribonucleoprotein complex. Recombinant SNM1 protein binds RNA in both gel retardation and Northwestern assays. Antibodies raised against bacterially expressed proteins identified four separate species in yeast whole cell extracts. Antibodies directed against the SNM1 protein immunoprecipitated RNase MRP RNA from whole-cell extracts without precipitating the structurally and functionally related RNase P RNA. We propose that the SNM1 protein is an essential and specific component of the RNase MRP ribonucleoprotein complex, the first unique protein of this complex to be identified.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Schmitt ME,Clayton DAdoi
10.1101/gad.8.21.2617subject
Has Abstractpub_date
1994-11-01 00:00:00pages
2617-28issue
21eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract::Activating JAK2 point mutations are implicated in the pathogenesis of myeloid and lymphoid malignancies, including high-risk B-cell acute lymphoblastic leukemia (B-ALL). In preclinical studies, treatment of JAK2 mutant leukemias with type I JAK2 inhibitors (e.g., Food and Drug Administration [FDA]-approved ruxolitinib...
journal_title:Genes & development
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journal_title:Genes & development
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pub_type: 评论,杂志文章,评审
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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doi:10.1101/gad.11.9.1194
更新日期:1997-05-01 00:00:00
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journal_title:Genes & development
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更新日期:2010-06-15 00:00:00
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pub_type: 杂志文章
doi:10.1101/gad.11.8.1023
更新日期:1997-04-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.21.2801
更新日期:1997-11-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.275453.115
更新日期:2016-02-15 00:00:00
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journal_title:Genes & development
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更新日期:2001-12-15 00:00:00
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journal_title:Genes & development
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更新日期:2010-09-01 00:00:00
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doi:10.1101/gad.11.5.663
更新日期:1997-03-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.10.1253
更新日期:1997-05-15 00:00:00
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journal_title:Genes & development
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doi:10.1101/gad.2.12b.1700
更新日期:1988-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.8.1157
更新日期:1989-08-01 00:00:00
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journal_title:Genes & development
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更新日期:2002-07-01 00:00:00
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