Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the cell cycle.

Abstract:

:Dexamethasone and etoposide both induce apoptosis in immature rat thymocytes. We investigated the dependence of apoptosis on the phase of the cell cycle after incubation with these drugs. Cell cycle progression was followed by a combination of pulse labelling with 5-bromo-2'-deoxyuridine (BrdU), labelling fixed cells with an anti-BrdU antibody and flow cytometry. Dexamethasone had little effect on the cell cycle progression of proliferating thymocytes, while etoposide caused cell cycle arrest. Normal and apoptotic thymocytes were separated by centrifugation on discontinuous Percoll gradients into four fractions (F1-F4). It was found that both dexamethasone and etoposide induced apoptosis in cells in G0/G1 and G2/M of the cell cycle, whereas only etoposide induced apoptosis of cells in S phase. These results demonstrated that dexamethasone induced apoptosis in quiescent cells while only etoposide could induce apoptosis in cells from the proliferative compartment. Following treatment of thymocytes with etoposide, some of the proliferating thymocytes (F1) were converted to cells with intermediate size and density (F3). We have recently identified these cells as a population of preapoptotic thymocytes, at an early stage of apoptosis. These cells then further progressed to fully apoptotic cells (F4). These data support the hypothesis that normal thymocytes (F1) became apoptotic (F4) via an intermediate population (F3).

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Fearnhead HO,Chwalinski M,Snowden RT,Ormerod MG,Cohen GM

doi

10.1016/0006-2952(94)90142-2

subject

Has Abstract

pub_date

1994-09-15 00:00:00

pages

1073-9

issue

6

eissn

0006-2952

issn

1873-2968

pii

0006-2952(94)90142-2

journal_volume

48

pub_type

杂志文章
  • Heat shock protein 70 protects against bleomycin-induced pulmonary fibrosis in mice.

    abstract::Idiopathic pulmonary fibrosis (IPF) involves infiltration of leucocytes, pulmonary injury, fibrosis and resulting pulmonary dysfunction. Myofibroblasts and transforming growth factor (TGF)-beta1 have been suggested to play a major role in the pathology and the myofibroblasts are derived from both lung epithelial cells...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2010.05.025

    authors: Tanaka K,Tanaka Y,Namba T,Azuma A,Mizushima T

    更新日期:2010-09-15 00:00:00

  • Combination of irinotecan (CPT11) and 5-fluorouracil with an analysis of cellular determinants of drug activity.

    abstract::We evaluated the combination SN38 (7-ethyl-10-hydroxycamptothecin) -5fluorouracil (5FU) +/- folinic acid (FA) on six human colon cancer cell lines expressing spontaneous sensitivity to both drugs. Tumoral parameters potentially related to drug sensitivity were investigated: topoisomerase I (topo I) cleavable complexes...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(98)00205-6

    authors: Pavillard V,Formento P,Rostagno P,Formento JL,Fischel JL,Francoual M,Etienne MC,Milano G

    更新日期:1998-11-15 00:00:00

  • 3-(13-Hydroxytridecyl)-1-[13-(3-pyridyl)tridecyl]pyridinium chloride (YM-53792), a novel inhibitor of NF-AT activation.

    abstract::A compound, YM-53792, was identified as an inhibitor of interleukin-2 (IL-2) gene promoter activity, using a Jurkat cell-based reporter system in which the luciferase gene is regulated by the IL-2 gene promoter. Production of IL-2, interleukin-4 (IL-4) and interleukin-5 (IL-5) from human peripheral blood mononuclear c...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(97)00289-x

    authors: Kuromitsu S,Fukunaga M,Lennard AC,Masuho Y,Nakada S

    更新日期:1997-11-01 00:00:00

  • Restored expression and activity of organic ion transporters rOAT1, rOAT3 and rOCT2 after hyperuricemia in the rat kidney.

    abstract::We previously reported that in hyperuricemic rats, renal impairment occurred and organic ion transport activity decreased, accompanied with a specific decrease in the expression of rat organic anion transporters, rOAT1 and rOAT3, and organic cation transporter, rOCT2. In the present study, we investigated the reversib...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2004.12.004

    authors: Habu Y,Yano I,Okuda M,Fukatsu A,Inui K

    更新日期:2005-03-15 00:00:00

  • Selective action of the iminosugar isofagomine, a pharmacological chaperone for mutant forms of acid-beta-glucosidase.

    abstract::Gaucher disease is a lysosomal glycolipid storage disorder characterized by defects in acid-beta-glucosidase (GlcCerase), the enzyme responsible for the catabolism of glucosylceramide. We recently demonstrated that isofagomine (IFG), an iminosugar that binds to the active site of GlcCerase, enhances the folding, trans...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2006.12.015

    authors: Steet R,Chung S,Lee WS,Pine CW,Do H,Kornfeld S

    更新日期:2007-05-01 00:00:00

  • GR-C/EBPα-IGF1 axis mediated azithromycin-induced liver developmental toxicity in fetal mice.

    abstract::Azithromycin is considered an effective drug to treat the perinatal mycoplasma infection. However, there is a lack of studies on developmental toxicity of azithromycin. In this study, we observed the developmental toxicity of fetal liver induced by prenatal azithromycin exposure (PAE) in mice and explored the potentia...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2020.114130

    authors: Liu K,Wang G,Li L,Chen G,Gong X,Zhang Q,Wang H

    更新日期:2020-10-01 00:00:00

  • Selective modifications in the de novo biosynthesis of retinal phospholipids and glycerides by propranolol or phentolamine.

    abstract::The effects of propranolol or phentolamine on the metabolism of phospholipids, diacylglycerol, and triacylglycerol were studied in the bovine retina in vitro. Lipid labeling was followed during short-term incubation of intact bovine retinas with [U-14C]glycerol and [1-14C]palmitic acid. Each of these precursors was re...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(82)90341-0

    authors: de Boschero MG,Bazan NG

    更新日期:1982-03-15 00:00:00

  • Different effects of oxytetracycline and doxycycline on mitochondrial protein synthesis in rat liver after long-term treatment.

    abstract::The tetracyclines inhibit specifically mitochondrial (mt) and bacterial protein synthesis when they are present in low concentrations (2-10 micrograms/ml). There is no difference between the various members of this group of antibiotics in this respect. In the present study, however, it is shown that the inhibitory eff...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(87)90126-2

    authors: van den Bogert C,Holtrop M,Melis TE,Roefsema PR,Kroon AM

    更新日期:1987-05-01 00:00:00

  • 3'-Azido-3'-deoxythymidine (AZT) inhibits thymidine phosphorylation in isolated rat liver mitochondria: a possible mechanism of AZT hepatotoxicity.

    abstract::3'-azido-3'-deoxythymidine (AZT) is a staple of highly active antiretroviral therapy (HAART). Prior to HAART, long-term use of high-dosage AZT caused myopathy, cardiomyopathy, and hepatotoxicity, associated with mitochondrial DNA depletion. As a component of HARRT, AZT causes cytopenias and lipodystrophy. AZT-5'-triph...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2006.01.003

    authors: Lynx MD,Bentley AT,McKee EE

    更新日期:2006-04-28 00:00:00

  • Inhibition of the release of prostaglandins, leukotrienes and lysosomal acid hydrolases from macrophages by selective inhibitors of lecithin biosynthesis.

    abstract::The release of the inflammatory mediators, prostaglandins (PGs), leukotrienes (LT) and lysosomal acid hydrolases (LAH), by macrophages is stimulated by endocytic stimuli such as zymosan. This process can be interfered with by specific inhibitors of phosphatidylcholine (PC) biosynthesis. The diphenylsulfone dapsone and...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(83)90568-3

    authors: Bonney RJ,Wightman PD,Dahlgren ME,Sadowski SJ,Davies P,Jensen N,Lanza T,Humes JL

    更新日期:1983-01-15 00:00:00

  • Overcoming chemotherapy resistance of ovarian cancer cells by liposomal cisplatin: molecular mechanisms unveiled by gene expression profiling.

    abstract::Previously we reported that liposomal cisplatin (CDDP) overcomes CDDP resistance of ovarian A2780cis cancer cells (Krieger et al., Int. J. Pharm. 389, 2010, 10-17). Here we find that the cytotoxic activity of liposomal CDDP is not associated with detectable DNA platination in resistant ovarian cancer cells. This sugge...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2013.01.028

    authors: Koch M,Krieger ML,Stölting D,Brenner N,Beier M,Jaehde U,Wiese M,Royer HD,Bendas G

    更新日期:2013-04-15 00:00:00

  • Cisplatin-induced apoptosis by translocation of endogenous Bax in mouse collecting duct cells.

    abstract::cis-platinum(II) (cis-diammine dichloroplatinum; cisplatin) is a potent antitumor compound that is widely used for the treatment of many malignancies. An important side-effect of cisplatin is nephrotoxicity, which results from injury to renal tubular epithelial cells and can be manifested as either acute renal failure...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(01)00748-1

    authors: Lee RH,Song JM,Park MY,Kang SK,Kim YK,Jung JS

    更新日期:2001-10-15 00:00:00

  • The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function.

    abstract::The human α7 neuronal nicotinic acetylcholine receptor gene (CHRNA7) is a candidate gene for schizophrenia and an important drug target for cognitive deficits in the disorder. Activation of the α7*nAChR, results in opening of the channel and entry of mono- and divalent cations, including Ca(2+), that presynaptically p...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2011.06.018

    authors: Araud T,Graw S,Berger R,Lee M,Neveu E,Bertrand D,Leonard S

    更新日期:2011-10-15 00:00:00

  • SGS742: the first GABA(B) receptor antagonist in clinical trials.

    abstract::The GABA(B) receptor antagonist SGS742 (CGP36742) displays pronounced cognition enhancing effects in mice, young and old rats and in Rhesus monkeys in active and passive avoidance paradigms, in an eight-arm radial maze and a Morris water maze and in a social learning task. SGS742 blocks the late inhibitory postsynapti...

    journal_title:Biochemical pharmacology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/j.bcp.2004.07.030

    authors: Froestl W,Gallagher M,Jenkins H,Madrid A,Melcher T,Teichman S,Mondadori CG,Pearlman R

    更新日期:2004-10-15 00:00:00

  • Broad specificity of human phosphoglycerate kinase for antiviral nucleoside analogs.

    abstract::Nucleoside analogs used in antiviral therapies need to be phosphorylated to their tri-phospho counterparts in order to be active on their cellular target. Human phosphoglycerate kinase (hPGK) was recently reported to participate in the last step of phosphorylation of cytidine L-nucleotide derivatives [Krishnan PGE, La...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2004.06.012

    authors: Gallois-Montbrun S,Faraj A,Seclaman E,Sommadossi JP,Deville-Bonne D,Véron M

    更新日期:2004-11-01 00:00:00

  • Cytochrome P-450-dependent mixed-function oxidase and glutathione S-transferase activities in spontaneous obesity-diabetes.

    abstract::The effect of non-insulin-dependent diabetes on the hepatic microsomal cytochrome P450-dependent mixed-function oxidase system and on cytosolic glutathione S-transferase activity was determined using the spontaneously obese-diabetic (ob/ob) mouse model. The activities of the xenobiotic-metabolizing cytochrome P450 pro...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(92)90724-w

    authors: Barnett CR,Abbott RA,Bailey CJ,Flatt PR,Ioannides C

    更新日期:1992-04-15 00:00:00

  • Differential modulation of brain benzodiazepine receptor subtypes by ricinelaidic acid in vitro.

    abstract::The C-18 hydroxy fatty acids ricinelaidic acid and ricinoleic acid diminish the oleic acid-stimulated agonist benzodiazepine binding in the rat brain in vitro. The oleic acid-induced enhancement of [3H]diazepam binding was completely abolished in membranes from the cerebellum, but only partially decreased in membranes...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(94)90138-4

    authors: Witt MR,Nielsen M

    更新日期:1994-02-11 00:00:00

  • Regulatory roles of adenylate cyclase and cyclic nucleotide phosphodiesterases 1 and 4 in interleukin-13 production by activated human T cells.

    abstract::We studied the activities of 3',5'-adenosine-cyclic monophosphate (cAMP)- synthesizing adenylate cyclase (AC) and cAMP-hydrolyzing cyclic nucleotide phosphodiesterase (PDE) in phytohemagglutinin (PHA)- or anti-CD3 plus anti-CD28-stimulated human T cells, and examined their roles in interleukin-13 (IL-13) production. T...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(01)00688-8

    authors: Kanda N,Watanabe S

    更新日期:2001-08-15 00:00:00

  • Azalanstat (RS-21607), a lanosterol 14 alpha-demethylase inhibitor with cholesterol-lowering activity.

    abstract::Agents that inhibit hepatic cholesterol biosynthesis reduce circulating cholesterol levels in experimental animals and humans, and may be of pharmacological importance in the prevention of atherosclerosis. Azalanstat (RS-21607), a synthetic imidazole, has been shown to inhibit cholesterol synthesis in HepG2 cells, hum...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(95)00152-p

    authors: Burton PM,Swinney DC,Heller R,Dunlap B,Chiou M,Malonzo E,Haller J,Walker KA,Salari A,Murakami S

    更新日期:1995-08-08 00:00:00

  • Unequivocal synthesis and characterisation of dopamine 3- and 4-O-sulphates.

    abstract::The major metabolic products of the endogeneous catecholamine dopamine are its 3- and 4-O-sulphates which have also been implicated as intermediates in noradrenaline biosynthesis. Because of the unsatisfactory status of the literature concerning the synthesis, isolation, purity and characterisation of the dopamine O-s...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(82)90115-0

    authors: Osikowska BA,Idle JR,Swinbourne FJ,Sever PS

    更新日期:1982-07-01 00:00:00

  • Isoliquiritigenin inhibits IkappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells.

    abstract::Isoliquiritigenin (ILTG) is a flavonoid with chalcone structure (4,2',4'-trihydroxychalcone), an active component present in plants like Glycyrrhiza and Dalbergia which showed various biological activities including anti-inflammatory, anti-carcinogenic and antihistamic. As very little is known in regard to the underly...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2007.01.015

    authors: Kumar S,Sharma A,Madan B,Singhal V,Ghosh B

    更新日期:2007-05-15 00:00:00

  • Differential effects of in vitro peroxidation on peripheral- and central-type benzodiazepine receptors. Protection by diverse antioxidants.

    abstract::The influence of various concentrations of ferrous iron and ascorbate on in vitro peroxidation and drug binding of diverse membrane preparations (cerebral cortex and liver) was studied. Peroxidation was not simply dose-related to ascorbate and ferrous iron, but a complex relationship between iron and ascorbate when ad...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(95)02058-6

    authors: Courtiere A,Molard F,Reybaud J

    更新日期:1995-11-27 00:00:00

  • Inhibitory effect of the natural product betulin and its derivatives against the intracellular bacterium Chlamydia pneumoniae.

    abstract::Chlamydia pneumoniae is a universal pathogen that has been indicated to play a part in the development of asthma, atherosclerosis and lung cancer. The complete eradication of this intracellular bacterium is in practice impossible with the antibiotics that are currently in use and studies on new antichlamydial compound...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2010.06.051

    authors: Salin O,Alakurtti S,Pohjala L,Siiskonen A,Maass V,Maass M,Yli-Kauhaluoma J,Vuorela P

    更新日期:2010-10-15 00:00:00

  • Effect of tuamine, heptaminol and two analogues on uptake and release of catecholamines in cultured chromaffin cells.

    abstract::The effects of tuamine (1-methylhexylamine), a sympathomimetic compound with hypertensive properties, heptaminol (6-amino-2-methyl-2-heptanol), an aliphatic amine with pressor properties, and two structural analogues of tuamine on high-affinity Na(+)-dependent noradrenaline uptake and on nicotine-evoked release were e...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(90)90322-c

    authors: Delicado EG,Fideu MD,Miras-Portugal MT,Pourrias B,Aunis D

    更新日期:1990-08-15 00:00:00

  • Cytotoxicity of BMS-181174. Effects of hypoxia, dicoumarol, and repair deficits.

    abstract::The mitomycin C (MC) analog BMS-181174 (previously designated as BMY25067) has been shown to be active against a variety of solid tumors in mice. The activity of this compound against tumor cell lines resistant to MC and the different toxicity profiles of BMS-181174 and MC suggested that there may be significant diffe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(95)00264-z

    authors: Rockwell S,Kemple B,Kelley M

    更新日期:1995-10-12 00:00:00

  • Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone body-utilizing enzyme, in human brain.

    abstract::Acetoacetyl-CoA synthetase (AACS, acetoacetate-CoA ligase, EC 6.2.1.16) is a ketone body-utilizing enzyme, the physiological role of which remains unclear yet in mammals, particularly has never been studied in human. In order to investigate the tissue distribution of AACS in human, cDNA encoding AACS was isolated from...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0006-2952(02)01656-8

    authors: Ohgami M,Takahashi N,Yamasaki M,Fukui T

    更新日期:2003-03-15 00:00:00

  • Synergistic interaction of a chloroquine metabolite with chloroquine against drug-resistant malaria parasites.

    abstract::We have previously shown that structural modification of chlorpromazine to introduce a basic side chain converts this chloroquine (CQ) resistance-reversing agent into a compound that has activity against Plasmodium falciparum in vitro. In an effort to further dissect the structural features that determine quinoline an...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.bcp.2003.12.005

    authors: Kalkanidis M,Klonis N,Tschan S,Deady LW,Tilley L

    更新日期:2004-04-01 00:00:00

  • Exploiting the balance between life and death: targeted cancer therapy and "oncogenic shock".

    abstract::Rational approaches to targeted cancer therapy have begun to predominate the pipelines of oncology drug development. Our rapidly increasing understanding of the "wiring" of tumor cells and the vulnerabilities of such cells that can potentially be exploited through targeted treatments has opened up enormous opportuniti...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.bcp.2010.03.001

    authors: Sharma SV,Settleman J

    更新日期:2010-09-01 00:00:00

  • Cationic environment and secretin secretion in canine duodenal mucosa in vitro.

    abstract::We examined the effects of the divalent cation calcium (Ca2+) and the monovalent cations potassium (K+) and sodium (Na+) and different modalities that affect the fluxes of these cations on immunoreactive secretin (IRS) secretion from canine duodenal mucosa in vitro. In the absence of extracellular Ca2+, the basal IRS ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(88)90505-9

    authors: Murthy SN,Lavy A

    更新日期:1988-03-15 00:00:00

  • Novel mode of cytotoxicity obtained by coupling inactive anthracycline to a polymer.

    abstract::The inactive anthracycline analog 4-demethoxy-7,9-di-epi-daunorubicin was covalently coupled to polyglutaraldehyde microspheres. The polymer-bound analog acquired significant cytostatic activity as evaluated with doxorubicin resistant and sensitive murine L1210 leukemia cells. A suggested multiple membrane interaction...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章

    doi:10.1016/0006-2952(84)90315-0

    authors: Rogers KE,Tökés ZA

    更新日期:1984-02-15 00:00:00