Regulation of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF-A) expression in podocytes.

Abstract:

BACKGROUND:Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF-A) is expressed constitutively in the adult glomerular podocytes at high levels; however, the regulation of its production is unclear. Recent data from podocyte-specific knockout mice suggest that VPF/VEGF-A is critical for the proper maintenance of glomerular filtration barrier and the glomerular endothelial fenestrae. We hypothesized that the glomerular basement membrane (GBM) matrix-podocyte interaction may play a role in the constitutive expression of VPF/VEGF-A in the adult glomerulus. METHODS:VPF/VEGF-A mRNA levels in a human podocyte cell line grown in the presence of various extracellular matrices were quantitated by real-time polymerase chain reaction (PCR) experiments. VPF/VEGF-A protein levels in the culture supernatant from the same conditions were measured by enzyme-linked immunosorbent assay (ELISA). Promoter activity of VPF/VEGF-A gene in these cells was performed by transfecting full length (2.6 kb) VPF/VEGF-A promoter, which is fused with luciferase reporter gene. Immunoprecipitation and Western blot experiments were carried out in order to detect the association of hypoxia-inducible factor-alpha (HIF-alpha) and p300 in podocyte cells. RESULTS:In this study, we provide preliminary evidence that signaling through the extracellular matrix proteins and, in particular, laminin and its receptor alpha(3)beta(1) integrin may regulate VPF/VEGF-A production in cultured podocytes in vitro. We also present data that increased activity of the transcription factor HIF-alphas in podocyte is not related to hypoxia and may lead to up-regulation of VPF/VEGF-A transcription. The classical type protein kinase C (PKC) may be a potential intermediate signaling molecule in this event. CONCLUSION:These data suggest a novel nonhypoxic regulation of VPF/VEGF-A production in the glomerulus of the kidney during physiologic states. These observations may form the basis of more elaborate studies that will finally provide the detailed signaling pathway for VPF/VEGF-A synthesis in podocytes and will help our understanding of the pathogenesis of various VPF/VEGF-A-related diseases in the glomerulus of the kidney.

journal_name

Kidney Int

journal_title

Kidney international

authors

Datta K,Li J,Karumanchi SA,Wang E,Rondeau E,Mukhopadhyay D

doi

10.1111/j.1523-1755.2004.00910.x

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

1471-8

issue

4

eissn

0085-2538

issn

1523-1755

pii

S0085-2538(15)50214-7

journal_volume

66

pub_type

杂志文章
  • Mechanisms of altered renal perfusion in the early stage of obstructive jaundice.

    abstract::Mechanisms responsible for renal hemodynamic alterations were studied in jaundiced (by bile-duct ligation) rabbits (BDL) 10 days after ligation of the biliary tract, in comparison with sham-operated rabbits (SO). Arterial hematocrit, plasma volume, blood pressure, abdominal inferior vena cava pressure, and heart rate ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1980.25

    authors: Hishida A,Honda N,Sudo M,Nagase M

    更新日期:1980-02-01 00:00:00

  • Altered membrane ionic permeability in a rat model of chronic renal failure.

    abstract::Acute elevations in intracellular adenosine 3',5'-cyclic monophosphate (cAMP) concentrations are known to increase ionic chloride permeability in diverse tissues. To determine if chronic endogenous increases in cAMP are associated with sustained alterations in membrane ionic permeabilities, renal cortical brush border...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1992.290

    authors: London RD,Lipkowitz MS,Abramson RG

    更新日期:1992-08-01 00:00:00

  • Regulation of mesangial cell proliferation.

    abstract::Regardless of the source of injury, an imbalance in the control of mesangial cell proliferation appears to play a direct role in the degree of progressive renal injury and glomerulosclerosis. Some of the regulatory mechanisms include specific soluble or non-soluble extracellular factors and a complex array of receptor...

    journal_title:Kidney international

    pub_type: 杂志文章,评审

    doi:10.1046/j.1523-1755.1999.00710.x

    authors: Schöcklmann HO,Lang S,Sterzel RB

    更新日期:1999-10-01 00:00:00

  • Malignancy after kidney transplantation: still a challenge.

    abstract::Long-term complications of continuous immunosuppression still remain a serious threat and are currently drawing the attention of transplant physicians. Wimmer et al. show that malignancy occurs approximately fourfold more frequently in renal-transplant recipients than in a normal control population. Besides immunosupp...

    journal_title:Kidney international

    pub_type: 评论,杂志文章

    doi:10.1038/sj.ki.5002306

    authors: Bosmans JL,Verpooten GA

    更新日期:2007-06-01 00:00:00

  • Time to consider ACE insertion/deletion genotypes and individual renoprotective treatment in diabetic nephropathy?

    abstract::One reason for the inadequacy of current renoprotective therapy and the persistent poor renal prognosis in diabetic nephropathy is the large interindividual variation in response to treatment. Genetic as well as non-genetic factors are known to influence treatment efficacy. This Commentary summarizes the impact of the...

    journal_title:Kidney international

    pub_type: 评论,杂志文章

    doi:10.1038/sj.ki.5000283

    authors: Jacobsen PK,Tarnow L,Parving HH

    更新日期:2006-04-01 00:00:00

  • Assessment of glomerular filtration rate in addition to albuminuria is important in managing type II diabetes.

    abstract::Although much emphasis has been placed on screening for albuminuria in type II diabetic patients, less attention has been focused on the role of glomerular filtration rate (GFR) in the assessment of risk. Herein, we examined the association between GFR and vascular complications in a consecutive cohort of 5174 type II...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/sj.ki.5000061

    authors: Kong AP,So WY,Szeto CC,Chan NN,Luk A,Ma RC,Ozaki R,Ng VW,Ho CS,Lam CW,Chow CC,Cockram CS,Chan JC,Tong PC

    更新日期:2006-01-01 00:00:00

  • A risk allele for focal segmental glomerulosclerosis in African Americans is located within a region containing APOL1 and MYH9.

    abstract::Genetic variation at the MYH9 locus is linked to the high incidence of focal segmental glomerulosclerosis (FSGS) and non-diabetic end-stage renal disease among African Americans. To further define risk alleles with FSGS we performed a genome-wide association analysis using more than one million single-nucleotide polym...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.2010.251

    authors: Genovese G,Tonna SJ,Knob AU,Appel GB,Katz A,Bernhardy AJ,Needham AW,Lazarus R,Pollak MR

    更新日期:2010-10-01 00:00:00

  • Pivotal role of xanthine oxidase in the initiation of tubulointerstitial renal injury in rats with hyperlipidemia.

    abstract::Hyperlipidemia can induce or aggravate renal tubulointerstitial injury. Experiments in a complex rat model with chronic glomerulonephritis and long-standing, coexisting hyperlipidemia suggested that induction of xanthine oxidase (XO), with increased oxygen radical generation, is involved in aggravation of tubulointers...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/sj.ki.5000121

    authors: Gwinner W,Scheuer H,Haller H,Brandes RP,Groene HJ

    更新日期:2006-02-01 00:00:00

  • A molecular morphometric approach to diabetic kidney disease can link structure to function and outcome.

    abstract::Diabetic kidney disease is the leading cause of kidney failure. However, studies of molecular mechanisms of early kidney damage are lacking. Here we examined for possible linkage between transcriptional regulation and quantitative structural damage in early diabetic kidney disease in Pima Indians with type 2 diabetes....

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1016/j.kint.2017.08.013

    authors: Nair V,Komorowsky CV,Weil EJ,Yee B,Hodgin J,Harder JL,Godfrey B,Ju W,Boustany-Kari CM,Schwarz M,Lemley KV,Nelson PJ,Nelson RG,Kretzler M

    更新日期:2018-02-01 00:00:00

  • Induction of stress response proteins and experimental renal ischemia/reperfusion.

    abstract:BACKGROUND:The induction of stress response (heat shock) proteins (HSPs) is a highly conserved response that protects many cell types from diverse physiological and environmental stressors. We tested the hypothesis that the induction of HSPs is protective in experimental renal ischemia/reperfusion injury. METHODS:The ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.2001.0590051798.x

    authors: Kelly KJ,Baird NR,Greene AL

    更新日期:2001-05-01 00:00:00

  • Treatment of hepatitis B virus-associated membranous nephropathy with recombinant alpha-interferon.

    abstract::An open, randomized trial study on the therapeutic effect of recombinant alpha-interferon (IFN alpha) in 40 patients with hepatitis B virus membranous nephropathy (HBVMN) was conducted. All were pathologically proven to have HBVMN which showed no response to corticosteroid treatment represented by persistent heavy pro...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1038/ki.1995.27

    authors: Lin CY

    更新日期:1995-01-01 00:00:00

  • Preserved leukocyte CD11b expression at the site of interstitial inflammation in patients with high-flux hemodiafiltration.

    abstract::The impact of high-flux hemodialysis on clinical outcomes remains controversial. We have previously shown that in vivo transmigrated leukocytes from patients with low-flux bioincompatible hemodialysis have an impaired capacity to upregulate CD11b at the site of interstitial inflammation. In the present study, we inves...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/sj.ki.5002090

    authors: Olsson J,Dadfar E,Paulsson J,Lundahl J,Moshfegh A,Jacobson SH

    更新日期:2007-03-01 00:00:00

  • The financial effects of kidney stone prevention.

    abstract::Prevention of nephrolithiasis (NL) is now medically feasible and widely recommended. However, diagnosis and treatment of remediable causes of stones requires testing and drugs that impose a cost; this cost is balanced by the presumed reductions in stone related events and medical encounters. In order to assess the bal...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章

    doi:10.1038/ki.1996.489

    authors: Parks JH,Coe FL

    更新日期:1996-11-01 00:00:00

  • Surveillance of alloantibodies after transplantation identifies the risk of chronic rejection.

    abstract::The monitoring of the levels of alloantibodies following transplantation might facilitate early diagnosis of chronic rejection (CR), the leading cause of renal allograft failure. Here, we used serial alloantibody surveillance to monitor patients with preoperative positive flow cytometric crossmatch (FCXM). Sixty-nine ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.2010.556

    authors: Kimball PM,Baker MA,Wagner MB,King A

    更新日期:2011-05-01 00:00:00

  • G protein beta3 subunit C825T polymorphism in primary IgA nephropathy.

    abstract:BACKGROUND:The T allele of the G protein beta3 subunit (GNB3) C825T polymorphism has been associated with increased signal transduction, increased activity of the kidney Na+/H+ exchanger, and also with late-onset essential hypertension. Hypertension is a strong independent risk factor for progression in IgA nephropathy...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1111/j.1523-1755.2004.00734.x

    authors: Thibaudin L,Berthoux P,Thibaudin D,Mariat C,Berthoux F

    更新日期:2004-07-01 00:00:00

  • Disparity between surface and deep nephron function early after renal ischemia.

    abstract::Experiments were performed using a variety of methods to assess the functional status of different nephron populations following 45 min of renal ischemia in the rat. Micropuncture techniques revealed that SNGFR and reabsorption in the surface nephrons are only modestly reduced after ischemia, whereas kidney GFR and re...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1983.122

    authors: Mason J,Welsch J,Takabatake T

    更新日期:1983-07-01 00:00:00

  • Cysteamine therapy delays the progression of nephropathic cystinosis in late adolescents and adults.

    abstract::Nephropathic cystinosis is a multisystem autosomal recessive disease caused by cystine accumulation, which is usually treated by oral cysteamine. In order to determine long-term effects of this therapy, we enrolled 86 adult patients (mean age 26.7 years) diagnosed with nephropathic cystinosis, 75 of whom received cyst...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.2011.277

    authors: Brodin-Sartorius A,Tête MJ,Niaudet P,Antignac C,Guest G,Ottolenghi C,Charbit M,Moyse D,Legendre C,Lesavre P,Cochat P,Servais A

    更新日期:2012-01-01 00:00:00

  • Cell surface aminopeptidase A and N activities in human glomerular epithelial cells.

    abstract::Cell surface aminopeptidases N (APN) and A (APA) have been characterized on cultured human glomerular epithelial cells and a SV40-transformed cell line derived from them. APN had a wide substrate specificity whereas APA only attacked peptides with an acidic N terminal amino acid. Both enzymes also differed by their se...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1992.227

    authors: Stefanovic V,Vlahovic P,Ardaillou N,Ronco P,Ardaillou R

    更新日期:1992-06-01 00:00:00

  • Blood pressure and long-term mortality in United States hemodialysis patients: USRDS Waves 3 and 4 Study.

    abstract:BACKGROUND:The long-term prognostic associations of pre- and post-dialysis blood pressures, interdialytic weight gain, and antihypertensive use in hemodialysis patients are unclear. METHODS:The United States Renal Data System (USRDS) Dialysis Morbidity and Mortality Waves 3 and 4 Study, a randomly generated sample of ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.2002.00636.x

    authors: Foley RN,Herzog CA,Collins AJ,United States Renal Data System.

    更新日期:2002-11-01 00:00:00

  • KDIGO guideline for the care of kidney transplant recipients will be resource challenged.

    abstract::The Executive Summary of the KDIGO Clinical Practice Guideline for the Care of Kidney Transplant Recipients is presented in this issue of Kidney International. This guideline will require careful attention for implementation in the context of available resources within each country, especially those with underdevelope...

    journal_title:Kidney international

    pub_type: 评论,杂志文章

    doi:10.1038/ki.2009.468

    authors: Delmonico FL

    更新日期:2010-02-01 00:00:00

  • Elevated glucose alters paracellular transport of cultured human proximal tubule cells.

    abstract::Cultures of human proximal tubule cells were exposed to elevated concentrations of glucose and dome formation was assessed over a 22 day period of growth. Cultures grown on 5.5 mM glucose formed five domes per microscopic field while those exposed to elevated glucose concentrations (11.0 mM to 27.5 mM) formed only two...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1989.5

    authors: Hazen-Martin DJ,Sens MA,Detrisac CJ,Blackburn JG,Sens DA

    更新日期:1989-01-01 00:00:00

  • Nephritogenicity of proteoglycans. III. Mechanism of immune deposit formation.

    abstract::Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepi...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1988.166

    authors: Makino H,Lelongt B,Kanwar YS

    更新日期:1988-08-01 00:00:00

  • Glomerular angiotensinase A in the rat: increase of enzyme activity following renal ablation.

    abstract::Angiotensinase A (aminopeptidase A; ATA) is an angiotensin II splitting exopeptidase, which is localized in endothelial and epithelial cells of the glomerular tuft. In order to investigate the influence of a reduction in renal mass on enzyme activity, ATA activity was measured in isolated rat glomeruli five and 14 wee...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.1990.283

    authors: Wolf G,Thaiss F,Scherberich JE,Schoeppe W,Stahl RA

    更新日期:1990-11-01 00:00:00

  • Expansion of cytolytic CD4+CD28- T cells in end-stage renal disease.

    abstract::Cytomegalovirus (CMV) seropositivity is associated with increased risk for atherosclerotic disease in patients with end-stage renal disease. This association is due to a unique peripheral blood CD4(+) T cell population which lack CD28 (CD4(+)CD28(-) T cells). Here we found that this patient population has a significan...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1038/ki.2008.301

    authors: Betjes MG,Huisman M,Weimar W,Litjens NH

    更新日期:2008-09-01 00:00:00

  • Nature of glomerular dysfunction in pre-eclampsia.

    abstract:BACKGROUND:Pre-eclampsia is characterized by hypertension, proteinuria and edema. Simultaneous studies of kidney function and structure have not been reported. We wished to explore the degree and nature of glomerular dysfunction in pre-eclampsia. METHODS:Physiologic techniques were used to estimate glomerular filtrati...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.1998.00097.x

    authors: Lafayette RA,Druzin M,Sibley R,Derby G,Malik T,Huie P,Polhemus C,Deen WM,Myers BD

    更新日期:1998-10-01 00:00:00

  • Phenome-wide association analysis suggests the APOL1 linked disease spectrum primarily drives kidney-specific pathways.

    abstract::The relationship between commonly occurring genetic variants (G1 and G2) in the APOL1 gene in African Americans and different disease traits, such as kidney disease, cardiovascular disease, and pre-eclampsia, remains the subject of controversy. Here we took a genotype-first approach, a phenome-wide association study, ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1016/j.kint.2020.01.027

    authors: Bajaj A,Ihegword A,Qiu C,Small AM,Wei WQ,Bastarache L,Feng Q,Kember RL,Risman M,Bloom RD,Birtwell DL,Williams H,Shaffer CM,Chen J,Center RG,Denny JC,Rader DJ,Stein CM,Damrauer SM,Susztak K

    更新日期:2020-05-01 00:00:00

  • Combination therapy with ACE inhibitors and angiotensin II receptor blockers to halt progression of chronic renal disease: pathophysiology and indications.

    abstract::It is no a secret that we are confronted by an alarmingly increasing number of patients with progressive renal disease. There is ample evidence for the notion that angiotensin II (Ang II) is a major culprit in progression. The vasopeptide Ang II turned out to have also multiple nonhemodynamic pathophysiologic actions ...

    journal_title:Kidney international

    pub_type: 杂志文章,评审

    doi:10.1111/j.1523-1755.2005.00145.x

    authors: Wolf G,Ritz E

    更新日期:2005-03-01 00:00:00

  • Growth hormone receptor abundance in tibial growth plates of uremic rats: GH/IGF-I treatment.

    abstract:BACKGROUND:Children with chronic renal failure (CRF) exhibit growth retardation and a disturbed growth hormone/insulin-like growth factor-I (GH/IGF-I) axis. Treatment of children with CRF with GH or GH/IGF-I can partially restore linear growth. The molecular basis for decreased longitudinal growth is not known but may ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.2000.00141.x

    authors: Edmondson SR,Baker NL,Oh J,Kovacs G,Werther GA,Mehls O

    更新日期:2000-07-01 00:00:00

  • Cardiovascular disease in pediatric chronic dialysis patients.

    abstract:BACKGROUND:Little information is available regarding cardiac morbidity and mortality in children with end-stage renal disease. We sought to determine the incidence of cardiac morbidity and mortality in pediatric chronic dialysis patients. METHODS:Medicare incident pediatric (0 to 19 years) dialysis patients from 1991 ...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.2002.00472.x

    authors: Chavers BM,Li S,Collins AJ,Herzog CA

    更新日期:2002-08-01 00:00:00

  • Increased susceptibility to erythrocyte C5b-9 deposition and complement-mediated lysis in chronic renal failure.

    abstract:BACKGROUND:Decreased red blood cell survival contributes to the anemia of chronic renal failure patients. Because patients on chronic dialysis therapy are frequently exposed to excessive complement activation, we investigated the susceptibility of this patient population to erythrocyte C5b-9 deposition, complement-medi...

    journal_title:Kidney international

    pub_type: 杂志文章

    doi:10.1046/j.1523-1755.1999.00277.x

    authors: Himmelfarb J,McMonagle E,Holbrook D,Hakim R

    更新日期:1999-02-01 00:00:00