Abstract:
:Two proteinous factors, designated here as ram p25 GDP dissociation inhibitor (I) and (II) (ram-GDI (I) and (II)), were detected in the cytosolic fraction of rat spleen, which inhibited the initial dissociation of GDP from ram p25 produced by E. coli by causing characteristic lag. They had very weak effects on the rate of dissociation of GDP after the lag, and did not affect the mode of the dissociation of 5'-(3-O-thio)triphosphate (GTP gamma S) from ram p25. By gel filtration, the molecular masses of ram-GDI (I) and (II) were calculated to be 90 KDa and 40 KDa, respectively. These ram-GDIs did not affect the GDP-dissociation of Ha-ras protein produced in E. coli.
journal_name
Life Scijournal_title
Life sciencesauthors
Nagata K,Suzuki T,Okano Y,Nozawa Ydoi
10.1016/0024-3205(92)90455-xsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
1137-42issue
16eissn
0024-3205issn
1879-0631pii
0024-3205(92)90455-Xjournal_volume
50pub_type
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