Abstract:
:Cellular mechanisms underlying beta-adrenergic stimulation of the arrhythmogenic transient inward current (TI) were investigated by using a two-microelectrode voltage-clamp technique in rabbit cardiac Purkinje fibers. TI induced by elevating [Ca2+]o to 30 mmol/L and substituting [Na+]o with N-methyl-D-glucamine (NMG) chloride had a distinct reversal potential (EREV) of -25 mV, suggesting that Na(+)-Ca2+ exchange was not the charge carrier for TI. In the absence of [Na+]o, isoproterenol (ISO, 0.01 to 5.0 mumol/L) had no effect on either inward or outward TI or on the current-voltage relation of TI. However, ISO (0.1 mumol/L) significantly increased both inward and outward TIs without affecting the EREV of TI, if [Na+]o was present. Pretreatment with propranolol (0.2 mumol/L) or atenolol (0.2 mumol/L) abolished the stimulatory effects of ISO. Addition of propranolol (0.2 to 0.5 mumol/L) after the effects of ISO had developed caused only partial reversal of TI stimulation. This indicates persistence of stimulatory effects downstream from the initial agonist-receptor interaction. Forskolin (1 mumol/L), a direct adenylate cyclase activator, also strongly increased both inward and outward TI in the presence of [Na+]o. These effects also were abolished when [Na+]o was substituted by NMG. Inward and outward TIs enhanced by either ISO or forskolin were reversed by two putative Na(+)-Ca2+ exchange blockers, dodecylamine (20 mumol/L) and quinacrine (20 mumol/L). These results suggest that beta-adrenergic stimulation of TI is mediated by the Na(+)-Ca2+ exchange; stimulation likely involves phosphorylation of the exchanger or some factor that modulates exchanger activity.
journal_name
Circ Resjournal_title
Circulation researchauthors
Han X,Ferrier GRdoi
10.1161/01.res.76.4.664subject
Has Abstractpub_date
1995-04-01 00:00:00pages
664-74issue
4eissn
0009-7330issn
1524-4571journal_volume
76pub_type
杂志文章abstract:RATIONALE:Long noncoding RNAs (lncRNAs) constitute a novel class of noncoding RNAs that regulate gene expression. Although recent data suggest that lncRNAs may be associated with cardiac disease, little is known about lncRNAs in the setting of myocardial ischemia. OBJECTIVE:To measure lncRNAs in patients with myocardi...
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更新日期:1995-12-01 00:00:00
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更新日期:1975-01-01 00:00:00
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更新日期:1998-06-15 00:00:00
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pub_type: 杂志文章
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更新日期:1983-11-01 00:00:00
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更新日期:1982-03-01 00:00:00
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更新日期:2015-07-31 00:00:00
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pub_type: 杂志文章
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更新日期:2006-02-17 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.107.164699
更新日期:2008-01-04 00:00:00
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pub_type: 杂志文章
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更新日期:2005-10-14 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-02-05 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.67.2.253
更新日期:1990-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,收录出版
doi:10.1161/01.res.77.1.121
更新日期:1995-07-01 00:00:00
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更新日期:1989-12-01 00:00:00
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更新日期:1978-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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更新日期:2013-03-15 00:00:00
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pub_type: 杂志文章
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更新日期:2009-01-30 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2014-05-09 00:00:00
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pub_type: 杂志文章
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更新日期:1978-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-08-18 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000168650.23479.0c
更新日期:2005-05-27 00:00:00
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更新日期:2015-02-27 00:00:00