Abstract:
BACKGROUND:Two pancreatic cancer cell lines, the highly invasive and metastatic cell line PC-1.0 and the weakly invasive and rarely metastatic cell line PC-1, were established from a pancreatic ductal carcinoma induced by N-nitrosobis (2-oxopropyl) amine in a Syrian golden hamster. METHODS:The cancer cell dissociation activity in serum-free conditioned medium of PC-1.0 cells was partially purified using a heparin column, a hydroxylapatite column, anion exchange, and gel filtration high-performance liquid chromatography. Several biologic properties of the partially purified activity were evaluated. RESULTS:Two cell lines exhibited different growth morphologic changes in vitro: the weakly invasive cell line PC-1 formed islandlike colonies, and the highly invasive cell line PC-1.0 grew mainly as single cells. The conditioned medium of PC-1.0 cells induced dissociation of islandlike colonies and morphologic changes of PC-1 cells to elongated cells, with a high frequency of pseudopodia formation similar to the morphologic findings of PC-1.0 cells. The dissociation activity did not bind to the heparin column and had an apparent molecular mass of > 400 kDa, as deduced from gel filtration. Several immunoreactive proteinous bands were observed in immunoblotting analysis using a polyclonal blocking antibody. The partially purified activity enhanced cell motility, chemoinvasion, and cell adhesion to plastic plates and fibronectin. CONCLUSIONS:Highly invasive and metastatic PC-1.0 cells produce a soluble proteinous factor, called "dissociation factor" (DF), which induces cell dissociation of weakly invasive and rarely metastatic PC-1 cells. It seems likely that DF has a role in tumor invasion and metastasis.
journal_name
Cancerjournal_title
Cancerauthors
Kurizaki T,Egami H,Hirota M,Akagi J,Ohmachi H,Yamamoto S,Ogawa Mdoi
10.1002/1097-0142(19950315)75:6+<1554::aid-cncr282subject
Has Abstractpub_date
1995-03-15 00:00:00pages
1554-61issue
6 Suppleissn
0008-543Xissn
1097-0142journal_volume
75pub_type
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