Suppression of Bcl-2 messenger RNA production may mediate apoptosis after ionizing radiation, tumor necrosis factor alpha, and ceramide.

Abstract:

:Recent studies have proposed that tumor necrosis factor alpha (TNF-alpha) and ionizing radiation induce apoptosis by activating hydrolysis of sphingomyelin to ceramide. Bcl-2 and a related gene, Bcl-X, inhibit several forms of apoptosis. Herein, we report that internucleosomal DNA fragmentation, characteristic of apoptosis and induced by ionizing radiation, is accompanied by concomitant decreases in Bcl-2 and Bcl-X mRNA levels in HL-60 and U-937 human leukemia cells. Apoptotic DNA fragmentation after exposure to TNF-alpha and C2-ceramide was also associated with down-regulation of Bcl-2 mRNA in HL-60 and U-937 cells, while Bcl-X mRNA production was unaffected. These results suggest that modulation of Bcl-2 gene expression may be a target for ceramide-mediated apoptosis following exposure to ionizing radiation and TNF-alpha. Changes in Bcl-2 expression may be the basis for the interactive killing observed between radiation and TNF-alpha in some human and tumor cells.

journal_name

Cancer Res

journal_title

Cancer research

authors

Chen M,Quintans J,Fuks Z,Thompson C,Kufe DW,Weichselbaum RR

subject

Has Abstract

pub_date

1995-03-01 00:00:00

pages

991-4

issue

5

eissn

0008-5472

issn

1538-7445

journal_volume

55

pub_type

杂志文章
  • Evidence for selection of homogeneously staining regions in a human melanoma cell line.

    abstract::Cytogenetic analysis of tumor cells from a human malignant melanoma was performed on both the primary tumor colony-forming cells and a cell line (HA-A) established subsequently from the clonogenic population. Chromosome-banding analysis demonstrated identical karyotypic alterations in both the tumor colony-forming cel...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Trent JM,Thompson FH,Ludwig C

    更新日期:1984-01-01 00:00:00

  • Distinct structural domains within C19ORF5 support association with stabilized microtubules and mitochondrial aggregation and genome destruction.

    abstract::C19ORF5 is a sequence homologue of microtubule-associated proteins MAP1A/MAP1B of unknown function, except for its association with mitochondria-associated proteins and the paclitaxel-like microtubule stabilizer and candidate tumor suppressor RASSF1A. Here, we show that when overexpressed in mammalian cells the recomb...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-3865

    authors: Liu L,Vo A,Liu G,McKeehan WL

    更新日期:2005-05-15 00:00:00

  • Reactive Oxygen and Nitrogen Species-Induced Protein Modifications: Implication in Carcinogenesis and Anticancer Therapy.

    abstract::Cancer is a complex disorder extremely dependent on its microenvironment and highly regulated by multiple intracellular and extracellular stimuli. Studies show that reactive oxygen and nitrogen species (RONS) play key roles in cancer initiation and progression. Accumulation of RONS caused by imbalance between RONS gen...

    journal_title:Cancer research

    pub_type: 杂志文章,评审

    doi:10.1158/0008-5472.CAN-18-0980

    authors: Moldogazieva NT,Lutsenko SV,Terentiev AA

    更新日期:2018-11-01 00:00:00

  • Expression of a transfected H-2Kb gene in B16 cells correlates with suppression of liver metastases in triple immunodeficient mice.

    abstract::In vivo experiments performed with NIH (nu/nu, bg/bg, xid/xid) triple immunodeficient (TD) mice revealed the striking ability of i.v. injected B16-F1 and B16-F10 murine melanoma cells to colonize not only the lungs but also the liver of TD mice. Subsequently, B16 melanoma cell cultures, which express very low levels o...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Calorini L,Marozzi A,Byers HR,Waneck GL,Lee KW,Isselbacher KJ,Gattoni-Celli S

    更新日期:1992-07-15 00:00:00

  • Peroxisome proliferator-activated receptor gamma-mediated up-regulation of syndecan-1 by n-3 fatty acids promotes apoptosis of human breast cancer cells.

    abstract::Diets enriched in n-3 polyunsaturated fatty acids (n-3 PUFA) may protect against breast cancer but biochemical mechanisms are unclear. Our studies showed that the n-3 fatty acid docosahexaenoic acid (DHA) up-regulated syndecan-1 (SDC-1) in human breast cancer cells, and we tested the hypothesis that DHA-mediated up-re...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-2305

    authors: Sun H,Berquin IM,Owens RT,O'Flaherty JT,Edwards IJ

    更新日期:2008-04-15 00:00:00

  • Histone H3 and heat shock protein GRP78 are selectively cross-linked to DNA by photoactivated gilvocarcin V in human fibroblasts.

    abstract::Gilvocarcin V (GV) is an antitumor antibiotic with a coumarin-based aromatic structure that promotes protein-DNA cross-linking when photoactivated by near-UV light. We have now identified several proteins that are selectively cross-linked to DNA in human fibroblasts by photoactivated GV, using NH2-terminal amino acid ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Matsumoto A,Hanawalt PC

    更新日期:2000-07-15 00:00:00

  • Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival.

    abstract::Sequence alterations of mitotic checkpoint genes, hBUB1 and hBUBR1, were examined, and their gene transcripts were quantified using on-line, real-time quantitative reverse transcription-PCR in surgically resected human colorectal cancers and their neighboring normal tissues. Our results reveal a new hBUB1 missense mut...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Shichiri M,Yoshinaga K,Hisatomi H,Sugihara K,Hirata Y

    更新日期:2002-01-01 00:00:00

  • In vivo antitumor activity of interleukin 21 mediated by natural killer cells.

    abstract::Immunotherapy with high-dose interleukin (IL) 2 has been shown to successfully treat tumors in animal models and cause dramatic tumor regressions in some patients with metastatic melanoma, renal cell carcinoma, and non-Hodgkin's lymphoma. However, toxicity associated with IL-2 administration has compromised its widesp...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang G,Tschoi M,Spolski R,Lou Y,Ozaki K,Feng C,Kim G,Leonard WJ,Hwu P

    更新日期:2003-12-15 00:00:00

  • Human monoclonal antibodies directed against melanoma tumor-associated antigens.

    abstract::To analyze the humoral immune response to melanoma, human-mouse hybridomas were generated by the fusions of regional lymph node lymphocytes of patients with the mouse myeloma cell line M5. Six stable hybridomas were cloned from six separate lymphocyte parents obtained from three patients. Ascites were obtained from nu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kan-Mitchell J,Imam A,Kempf RA,Taylor CR,Mitchell MS

    更新日期:1986-05-01 00:00:00

  • Transforming growth factor beta induces clustering of HER2 and integrins by activating Src-focal adhesion kinase and receptor association to the cytoskeleton.

    abstract::It has been proposed that cross talk between integrin and growth factor receptor signaling such as ErbB2 (HER2) is required for activation of downstream effectors and ErbB2-mediated mammary tumorigenesis. Here we show that transforming growth factor beta (TGF-beta) induced focal adhesion kinase (FAK)-dependent cluster...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-2649

    authors: Wang SE,Xiang B,Zent R,Quaranta V,Pozzi A,Arteaga CL

    更新日期:2009-01-15 00:00:00

  • Effects of double and multiple doses of hydroxyurea on mouse duodenum and mammary tumors.

    abstract::Previous data on the effects of a single dose of hydroxyurea on C3H mouse duodenum and mammary tumors from a fast growing line (S102F) were used to predict times that may be optimal (i.e., minimize killing of the duodenal S-phase cells while enhancing the killing of tumor S-phase cells) for the administration of subse...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Dethlefsen LA,Sorensen SP,Riley RM

    更新日期:1975-03-01 00:00:00

  • Predicting response to radioimmunotherapy from the tumor microenvironment of colorectal carcinomas.

    abstract::Solid tumors have a heterogeneous pathophysiology, which directly affects antibody-targeted therapies. Here, we consider the influence of selected tumor parameters on radioimmunotherapy, by comparing the gross biodistribution, microdistribution, and therapeutic efficacy of either radiolabeled or fluorescently labeled ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-2967

    authors: El Emir E,Qureshi U,Dearling JL,Boxer GM,Clatworthy I,Folarin AA,Robson MP,Nagl S,Konerding MA,Pedley RB

    更新日期:2007-12-15 00:00:00

  • Gprc5a deletion enhances the transformed phenotype in normal and malignant lung epithelial cells by eliciting persistent Stat3 signaling induced by autocrine leukemia inhibitory factor.

    abstract::Signal transducers and activators of transcription 3 (Stat3) is activated by cytokines and growth factors in lung cancers and regulates expression of genes implicated in cell growth, survival, and transformation. Previously, we found that mice with a deletion of the G protein-coupled receptor, family C, group 5, membe...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-10-0518

    authors: Chen Y,Deng J,Fujimoto J,Kadara H,Men T,Lotan D,Lotan R

    更新日期:2010-11-01 00:00:00

  • Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue.

    abstract::Human and mouse paralogues of the evolutionarily conserved mammalian HRAD9 and Mrad9 cell cycle checkpoint control genes have been isolated and called HRAD9B and Mrad9B, respectively. HRAD9B encodes a protein that is 414 amino acids long and is 55% similar and 35% identical to the HRAD9 gene product. The Mrad9B protei...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hopkins KM,Wang X,Berlin A,Hang H,Thaker HM,Lieberman HB

    更新日期:2003-09-01 00:00:00

  • Immune-mediated arrest and reversal of established visceral metastases in athymic mice.

    abstract::The metastasizing MDAY-D2 tumor of DBA/2 mice disseminates in BALB/c allogeneic athymic nude (nu/nu) mice in a manner identical to that observed in the syngeneic host. Both the kinetics and organ distribution pattern of metastases from s.c. implants of MDAY-D2 are routinely predictable at any given tumor dose. BALB/c ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wiltrout RH,Frost P,Morrison MK,Kerbel RS

    更新日期:1979-10-01 00:00:00

  • A double-blind study on the effects of differing purified cellulose and pectin fiber diets on 1,2-dimethylhydrazine-induced rat colonic neoplasia.

    abstract::The incidence, distribution, size, and histopathology of colonic tumors induced by parenteral administration of 1,2-dimethylhydrazine were examined in rats fed a chemically defined fiber-free diet or nutritionally and calorically equivalent diets containing either 4.5 or 9.0% purified cellulose or pectin. This double-...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Freeman HJ,Spiller GA,Kim YS

    更新日期:1980-08-01 00:00:00

  • Effects of differentiating agents on cell surface expression of the breast carcinoma-associated DF3-P epitope.

    abstract::The DF3 antigen is a member of a family of high-molecular-weight glycoproteins aberrantly expressed in human breast carcinomas. Recent work has described the generation of a monoclonal antibody (MAb), designated DF3-P, that reacts with immature, underglycosylated precursors of DF3 antigen. Immunoperoxidase staining st...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Perey L,Hayes DF,Kufe D

    更新日期:1992-11-15 00:00:00

  • Purification of an anemia-inducing factor from human placenta and its application to diagnosis of malignant neoplasms.

    abstract::An anemia-inducing substance was isolated from the human placenta and designated placental anemia-inducing factor (PAIF). An amount of 2.8 mg was purified from about 70 total placentas (total wet weight, 42 kg). PAIF caused hemolysis of human erythrocytes in vitro and reduced the number of erythrocytes in rabbits to 8...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Oh-Uti K,Inoue S,Minato S

    更新日期:1980-05-01 00:00:00

  • Neutralizing murine TGFβR2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis.

    abstract::Elevated levels of TGFβ are a negative prognostic indicator for patients diagnosed with pancreatic cancer; as a result, the TGFβ pathway is an attractive target for therapy. However, clinical application of pharmacologic inhibition of TGFβ remains challenging because TGFβ has tumor suppressor functions in many epithel...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-13-1807

    authors: Ostapoff KT,Cenik BK,Wang M,Ye R,Xu X,Nugent D,Hagopian MM,Topalovski M,Rivera LB,Carroll KD,Brekken RA

    更新日期:2014-09-15 00:00:00

  • Distribution of DNA polymerase Cm in normal and malignant human tissues.

    abstract::The distribution in human tissues has been determined of the new DNA polymerase activity designated DNA polymerase Cm, first isolated from the human melanoma cell line A-375. Tissue samples were fractionated by ion exchange chromatography on diethylaminoethyl cellulose and phosphocellulose and by affinity chromatograp...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Loewenstein PM,Lange GW,Gerard GF

    更新日期:1980-12-01 00:00:00

  • Elevated DNA repair capacity is associated with intrinsic resistance of lung cancer to chemotherapy.

    abstract::Non-small cell lung cancer (N-SCLC) is generally unresponsive to chemotherapy even without previous drug treatment, as opposed to small cell lung cancer (SCLC), which is initially responsive to chemotherapy. The mechanisms of this intrinsic resistance are unknown. This study was designed to investigate the role of DNA...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Zeng-Rong N,Paterson J,Alpert L,Tsao MS,Viallet J,Alaoui-Jamali MA

    更新日期:1995-11-01 00:00:00

  • Prognostic significance of HER2 and HER4 coexpression in childhood medulloblastoma.

    abstract::Recent in vitro studies of the epidermal growth factor receptor (EGFR) family have revealed complex signaling interactions involving the production of ligand-mediated heterodimers synergistic for the transformation of cells in vitro. In a series of 70 patients with childhood medulloblastoma, we have used immunohistoch...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Gilbertson RJ,Perry RH,Kelly PJ,Pearson AD,Lunec J

    更新日期:1997-08-01 00:00:00

  • Quantitative changes in tumor metabolism, partial pressure of oxygen, and radiobiological oxygenation status postradiation.

    abstract::Hypoxia is considered to be a major cause of tumor radioresistance. Reoxygenation of previously hypoxic areas after a priming dose of radiation is associated with an increase in tumor radiosensitivity. In a study of a hypoxic mammary carcinoma, 31P nuclear magnetic resonance spectra showed statistically significant in...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Koutcher JA,Alfieri AA,Devitt ML,Rhee JG,Kornblith AB,Mahmood U,Merchant TE,Cowburn D

    更新日期:1992-09-01 00:00:00

  • The significance of nonspecific injury for colon carcinogenesis in rats.

    abstract::A purse-string suture was put into the rat's cecum to form a "diverticulum." When the thread cut this stitch, the resultant extensive necrotic zone healed for a long time. The presence of a foreign body (ligature) provided a permanent source of injury to the cecal mucosa. The lesions caused an increase in [3H]thymidin...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Pozharisski KM

    更新日期:1975-12-01 00:00:00

  • Plants with potential to enhance significant tumor growth.

    abstract::Of 20,000 plant extracts submitted to the National Cancer Institute antitumor screens from 1960 to 1980, over 95% exhibited inadequate tumor-inhibiting activity or promoted tumor growth. Of these, 50 extracts representing 42 species showed significant levels of tumor growth enhancement compared to controls on the basi...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Caldwell ME,Brewer WR

    更新日期:1983-12-01 00:00:00

  • Topography of nonneoplastic and neoplastic cells of common origin.

    abstract::The possibility that neoplastic transformation may characteristically alter cell surface morphology prompted a comparison by scanning electron microscopy of nonneoplastic and tumorigenic cell lines from a single clone of mouse embryo cells. Among those studied by scanning electron microscopy, six lines of this clone p...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wetzel B,Sanford KK,Fox CH,Jones GM,Westbrook EW,Tarone RE

    更新日期:1977-03-01 00:00:00

  • Multilaminar endoplasmic reticulum and abnormal mitosis in Hodgkin tumor cells.

    abstract::A multilaminar alteration of endoplasmic reticulum (ER) has been observed in tumor cells of eight patients with Hodgkin's disease and a patient with histiocytic lymphoma. These multilaminar structures are more numerous in dividing cells and thus appear to arise primarily during mitosis. The stacked membranes in the mu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Parmley RT,Spicer SS,Garvin AJ

    更新日期:1976-05-01 00:00:00

  • Genetic alterations in primary bladder cancers and their metastases.

    abstract::Bladder cancer progression is thought to be associated with sequential genetic events. To search for the specific genetic changes associated with the metastatic process, comparative genomic hybridization was performed on 22 primary tumors and 24 metastases (10 distant and 14 nodal metastases) from 17 patients with sta...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hovey RM,Chu L,Balazs M,DeVries S,Moore D,Sauter G,Carroll PR,Waldman FM

    更新日期:1998-08-15 00:00:00

  • IFN-beta down-regulates the expression of DNA repair gene MGMT and sensitizes resistant glioma cells to temozolomide.

    abstract::Alkylating agents, such as temozolomide, are among the most effective cytotoxic agents used for malignant gliomas, but responses remain very poor. The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) plays an important role in cellular resistance to alkylating agents. IFN-beta can act as a drug sensiti...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-0036

    authors: Natsume A,Ishii D,Wakabayashi T,Tsuno T,Hatano H,Mizuno M,Yoshida J

    更新日期:2005-09-01 00:00:00

  • Mice transgenic for human carcinoembryonic antigen as a model for immunotherapy.

    abstract::Mice transgenic for the human carcinoembryonic antigen (CEA) gene were prepared for use as a preclinical model for immunotherapy. A 32.6-kb fragment containing the complete human CEA gene and flanking sequences was isolated from a genomic cosmid clone and used to produce transgenic C57BL/6 mice. A homozygous line was ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Clarke P,Mann J,Simpson JF,Rickard-Dickson K,Primus FJ

    更新日期:1998-04-01 00:00:00