Abstract:
:In this study we investigated the tumorigenicity, growth pattern and spontaneous metastatic ability of a series of nine human colorectal carcinoma cell lines after subcutaneous and intracaecal xenografting in nude mice. CaCo2 cells were found to be poorly tumorigenic to non-tumorigenic in either site; the other cell lines were tumorigenic in both sites. SW1116, SW480 and SW620 did not show local invasive in the NCI-H716 and LS174T cells were both invasive in the caecum, but only NCI-H716 was invasive in the subcutis. HT29 and 5583 (S and E) cells were invasive in the caecum and from that site metastatic to the lungs and/or the liver. HT29 and 5583S cells were both invasive in the subcutis, but 5583E cells were not. Of each category of in vivo behaviour in the caecum, one cell line was further investigated with regard to invasion in vitro (into embryonic chick heart fragments), E-cadherin expression in vivo and in vitro and in vitro production of u-PA and t-PA. These parameters were chosen in view of their purported role in extracellular matrix degradation and intercellular adhesion, which are all involved in the invasive and metastatic cascade. Invasion in vitro was not predictive for invasion or metastasis in vivo. In the cell line which showed invasion in embryonic chick heart tissue, heterogeneous E-cadherin expression was observed in vitro together with a relatively high production of u-PA. The non-invasive cell lines showed in vitro homogeneous expression of E-cadherin with a relatively low production of u-PA. In vivo expression of E-cadherin was either absent or heterogeneous. We conclude that: (1) colorectal carcinoma xenografts show site-specific modification of in vivo invasive and metastatic behaviour; (2) invasion in vitro does not correlate with invasion and metastasis in vivo; (3) in vitro non-invasion might be associated with homogeneous E-cadherin expression and low production of u-PA; (4) E-cadherin expression in vitro differs from E-cadherin expression in vivo. The results support the notion that the microenvironment in which cancer cells grow is one of the factors involved in the regulation of invasive and metastatic behaviour.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
de Vries JE,Dinjens WN,De Bruyne GK,Verspaget HW,van der Linden EP,de Bruïne AP,Mareel MM,Bosman FT,ten Kate Jdoi
10.1038/bjc.1995.55subject
Has Abstractpub_date
1995-02-01 00:00:00pages
271-7issue
2eissn
0007-0920issn
1532-1827journal_volume
71pub_type
杂志文章abstract:BACKGROUND:The purpose of this study was to identify prostate cancer (PC) oncogenic microRNAs (miRs) based on miR microarray and to investigate whether these oncogenic miRs may be useful as PC biomarkers. METHODS:Initially, we carried out miR microarray and real-time PCR using RWPE-1, PC-3, DU-145 and LNCaP cells. To ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.125
更新日期:2013-04-30 00:00:00
abstract::Angiotensin II, a main effector peptide in the renin-angiotensin system, acts as a growth-promoting and angiogenic factor via type 1 angiotensin II receptors (AT(1)R). We have recently demonstrated that angiotensin II enhanced tumour cell invasion and vascular endothelial growth factor (VEGF) secretion via AT1R in ova...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602961
更新日期:2006-02-27 00:00:00
abstract::Overexpression of the c-erbB-2 proto-oncogene in mammary carcinoma is frequently associated with amplification of the c-erbB-2 gene, but it also occurs from single-copy gene. Studies in mammary-derived cell lines have shown that, whether or not the gene is amplified, there is a 6- to 8-fold increase in the accumulatio...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.146
更新日期:1995-04-01 00:00:00
abstract::The pharmacokinetics (PK) and pharmacodynamics (PD) of metronomic irinotecan have not been studied in cancer patients. The aim of the study is to investigate the PK/PD profile of irinotecan/SN-38 administered by metronomic schedule. Twenty chemotherapy-refractory or chemotherapy-resistant patients with metastatic colo...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604311
更新日期:2008-04-22 00:00:00
abstract::Trastuzumab has significantly improved the overall survival of patients with HER2+ metastatic breast cancer (MBC). However, outcomes can vary, with patients progressing within 1 year of treatment or exceptional cases of complete response to trastuzumab for ≥10 years. Identification of the underlying genomic aberration...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0999-z
更新日期:2020-10-01 00:00:00
abstract::Tamoxifen is the current therapy of choice for patients with oestrogen receptor positive breast cancer, and it is currently under evaluation as a prophylactic for women at high risk of developing the disease. However, tamoxifen is also known to induce proliferative changes in the endometrium increasing the risk of dev...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600157
更新日期:2002-03-04 00:00:00
abstract::Angiopoietin-1 is an important regulator of endothelial cell survival. Angiopoietin-1 also reduces vascular permeability mediated by vascular endothelial growth factor. The effects of angiopoietin-1 on tumour growth and angiogenesis are controversial. We hypothesised that angiopoietin-1 would decrease tumour growth an...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600598
更新日期:2002-11-04 00:00:00
abstract::Patients with recurrent or refractory head and neck squamous cell carcinoma received cisplatin/epinephrine injectable gel or placebo gel injected directly into the clinically dominant tumour. The double-blind phase III trial comprised of up to 6 weekly treatments over 8 weeks, 4 weekly evaluation visits, and then mont...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/sj.bjc.6600588
更新日期:2002-10-21 00:00:00
abstract::Hormone concentrations in blood and total 12 h urine values were compared between 40 post-menopausal women with breast cancer and 40 control women in a study which carefully controlled for the possible confounding effects of age, weight and pregnancy history by individually matching cases and controls on these factors...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1990.56
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:The role of further hormone therapy in castration-resistant prostate cancer (CRPC) remains unclear. We performed a multi-centre randomised phase III study comparing the use of Dexamethasone, Aspirin, and immediate addition of Diethylstilbestrol (DAiS) vs Dexamethasone, Aspirin, and deferred (until disease pr...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2011.7
更新日期:2011-02-15 00:00:00
abstract::We assessed the feasibility and pharmacokinetics of high-dose infusional paclitaxel in combination with doxorubicin, cyclophosphamide, and peripheral blood progenitor cell rescue. Between October 1995 and June 1998, 63 patients with high-risk primary [stage II with >or= 10 axillary nodes involved, stage IIIA or stage ...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1054/bjoc.2001.1835
更新日期:2001-06-15 00:00:00
abstract:BACKGROUND:Rectal bleeding is a recognised early symptom of colorectal cancer. This study aimed to assess the diagnostic accuracy of symptoms, signs and diagnostic tests in patients with rectal bleeding in relation to risk of colorectal cancer in primary care. METHODS:Diagnostic accuracy systematic review. Medline (19...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1038/sj.bjc.6605426
更新日期:2010-01-05 00:00:00
abstract::HMGI-C belongs to the high-mobility-group-protein (HMG) family of architectural transcription factors and considerable interest has recently been shown in its expression in neoplastic tissues and apparent involvement in tumorigenesis. We could previously demonstrate an expression of HMGI-C mRNA in the peripheral blood...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600935
更新日期:2003-05-06 00:00:00
abstract::We examined polymorphisms in exons 3 and 4 of microsomal epoxide hydrolase in 101 patients with colon cancer and compared the results with 203 control samples. The frequency of the exon 3 T to C mutation was higher in cancer patients than in controls (odds ratio 3.8; 95% confidence intervals 1.8-8.0). This sequence al...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690028
更新日期:1999-01-01 00:00:00
abstract::Common warts could indicate cervical cancer susceptibility, as both are caused by human papillomavirus (HPV). Eczema was also investigated, as atopic eczema has been negatively associated with warts, but non-atopic eczema may be associated with compromised host defences, as observed in patients with HIV, suggesting in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600585
更新日期:2002-10-21 00:00:00
abstract::Recent studies indicate that cancer antigen 125 (CA125) response criteria tend to overestimate a tumour reduction measured by standard WHO response criteria in recurrent epithelial ovarian carcinoma. The aim of the study was to validate the recently introduced GCIG (The Gynaecological Cancer Intergroup) CA125 response...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/sj.bjc.6601501
更新日期:2004-01-26 00:00:00
abstract::Primary liver cancer, predominantly consisting of hepatocellular carcinoma (HCC), is one of the most common and aggressive human malignancies worldwide. MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression post-transcriptionally. Emerging evidence indicates that miRNAs are often deregu...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6606010
更新日期:2011-01-18 00:00:00
abstract::This study was undertaken to investigate the intracellular induction of reactive oxygen species (ROS) by cis-dichlorodiammineplatinum (CDDP) and the augmentation of their cytotoxicity in bladder cancer cells (KU7) by enhancement of ROS generation by the glutathione (GSH) depletors buthionine sulphoximine (BSO) and die...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.363
更新日期:1997-01-01 00:00:00
abstract::Microcolonies were obtained by culturing cells of two human osteosarcoma lines (OHS and KPDX) and one human melanoma line (WIX-c) for either 24 or 72 h. The microcolonies were treated with either alpha-particle radiation emitted by the 211At-labelled monoclonal antibody (MAb) TP-3 or external beam X-rays. Survival of ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.196
更新日期:1994-06-01 00:00:00
abstract::This paper illustrates the efficacy of DNA vaccination through electroporation in the prevention of oral transplantable carcinoma in Syrian hamsters. At 21 and 7 days before tumour challenge, 19 hamsters were vaccinated with plasmids coding for the extracellular and transmembrane domains of rat HER-2 receptor (EC-TM p...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602853
更新日期:2005-11-28 00:00:00
abstract::Hepatic arterial floxuridine (HAI) in 35 patients with systemic fluorouracil/folinic acid-resistant colorectal liver metastases achieved a 14% partial response and 26% disease stabilization rate, with a median response duration of 7 months from onset of HAI. ...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1998.627
更新日期:1998-10-01 00:00:00
abstract::Reductions in cell-cell adhesion and stromal and vascular invasion are essential steps in the progression from localized malignancy to metastatic disease. In this study, changes in the expression of the components of the E-cadherin-catenin cell adhesion complex have been investigated using immunohistochemical techniqu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.252
更新日期:1997-01-01 00:00:00
abstract::To design hybrid tumour necrosis factor-alpha (TNF-alpha) applicable to systemic anti-tumour therapeutic use, we assessed the relationships among the molecular size of hybrid TNF-alpha, in vitro bioactivity and in vivo anti-tumour potency. Natural human TNF-alpha was covalently modified with polyethylene glycol (PEG) ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.495
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND:Overexpression of plasma membrane multi-drug resistance protein 1 (MRP-1) can lead to multidrug resistance. In this study, we describe for the first time the expression of mitochondrial MRP-1 in untreated human normal and cancer cells and tissues. METHODS:MRP-1 expression and subcellular localisation in nor...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.40
更新日期:2012-03-13 00:00:00
abstract::The incidence of oncofoetal antigens has been reported to be increased in patients with gynaecological cancers. In this study the incidence of CEA, AFP, and hCG (beta subunit) were studied in patients with adenocarcinoma of the ovary, adenocarcinoma of the cervix, and squamous-cell carcinoma of the cervix. Using a low...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1981.198
更新日期:1981-09-01 00:00:00
abstract::Aminoglutethimide without glucocorticoid has been shown to be a clinically effective treatment for postmenopausal breast cancer in low dosage (250 mg day-1). The mechanism of action of this approach is thought to be the inhibition of peripheral aromatase, the enzyme which converts androstenedione to oestrone. The acti...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1985.144
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:Cervical cancer screening coverage remains insufficient in most countries. Our objective was to assess whether in-home vaginal self-sampling with a dry swab for high-risk human papillomavirus (HR-HPV) testing is effective and cost-effective in increasing participation in cervical cancer screening. METHODS:I...
journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1038/bjc.2014.510
更新日期:2014-11-25 00:00:00
abstract::The zinc finger protein glioma-associated oncogene homologue 1 (Gli-1) is a critical component of the Hedgehog (Hh) signalling pathway, which is essential for morphogenesis and stem-cell renewal, and is dysregulated in many cancer types. As data were not available on the role of Gli-1 expression in oesophageal cancer ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604361
更新日期:2008-05-20 00:00:00
abstract::The effects of interferon-alpha (IFN) plus tamoxifen (TMX) in the treatment of advanced breast cancer were assessed. Changes of in vivo biologic determinants including hormone receptors, P24 protein, Ki-67 and growth factor expression were evaluated. Seven patients with advanced, heavily pretreated, breast cancer with...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.339
更新日期:1993-08-01 00:00:00
abstract::The largest single underlying cause of hepatocellular carcinoma (HCC) worldwide is hepatitis B virus (HBV) infection. Hepatitis B virus increases cellular oxidative stress and the development of HCC occurs after a long latency period. The study was carried out to determine whether mitochondrial DNA abnormalities were ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602496
更新日期:2005-04-11 00:00:00