Effects of buoyancy-driven convection on nucleation and growth of protein crystals.

Abstract:

:Protein crystallization has been studied in presence or absence of buoyancy-driven convection. Gravity-driven flow was created, or suppressed, in protein solutions by means of vertically directed density gradients that were caused by generating suitable temperature gradients. The presence of enhanced mixing was demonstrated directly by experiments with crustacyanin, a blue-colored protein, and other materials. Combined with the vertical tube position the enhanced convection has two main effects. First, it reduces the number of nucleated hen-egg-white lysozyme (HEWL) crystals, as compared with those in a horizontal capillary. By enabling better nutrition from the protein in the solution, convection results in growth of fewer larger HEWL crystals. Second, we observe that due to convection, trypsin crystals grow faster. Suppression of convection, achieved by decreasing solution density upward in the capillary, can to some extent mimic conditions of growth in microgravity. Thus, impurity supply, which may have a detrimental effect on crystal quality, was avoided.

journal_name

Ann N Y Acad Sci

authors

Nanev CN,Penkova A,Chayen N

doi

10.1196/annals.1324.001

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

1-9

eissn

0077-8923

issn

1749-6632

pii

1027/1/1

journal_volume

1027

pub_type

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