Abstract：
:Hyperforin is an important antidepressant constituent of Hypericum perforatum (St. John's wort). Cell cultures of the related species H. calycinum were found to contain the homologue adhyperforin and to a low extent hyperforin, when grown in BDS medium in the dark. Adhyperforin formation paralleled cell culture growth. Cell-free extracts from the cell cultures contained isobutyrophenone synthase activity catalyzing the condensation of isobutyryl-CoA with three molecules of malonyl-CoA to give phlorisobutyrophenone, i.e. the hyperforin skeleton. The formation of the hyperforins during cell culture growth was preceded by an increase in isobutyrophenone synthase activity. The cell cultures also contained benzophenone synthase and chalcone synthase activities which are involved in xanthone and flavonoid biosyntheses, respectively. The three type III polyketide synthases were separated by anion exchange chromatography.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Klingauf P,Beuerle T,Mellenthin A,El-Moghazy SA,Boubakir Z,Beerhues Ldoi
10.1016/j.phytochem.2004.11.003subject
Has Abstractpub_date
2005-01-01 00:00:00pages
139-45issue
2eissn
0031-9422issn
1873-3700pii
S0031-9422(04)00591-6journal_volume
66pub_type
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