Abstract：
:To determine whether postmitotic neurons can be immortalized by oncogenic transduction, we used two approaches involving conditional expression of a temperature-sensitive SV40 large T antigen (Tts). Initially, Tts was introduced into E17 rat embryonal hippocampal cells that were then cultured at the non-permissive temperature to enrich for postmitotic pyramidal neurons, and subsequently cloned at the permissive temperature. One clonal line (HMR10-3) expressed neuron-specific proteins upon differentiation, was capable of generating action potentials, and formed synapses with primary rat neurons in co-culture. Replating of these postmitotic cells at the permissive temperature resulted in reversible loss of neurofilament expression. Conditionally immortalized cell lines were also generated from the brain of an adult mouse carrying an inducible Tts transgene. These lines proliferated in a T antigen-dependent manner and expressed neuron-specific proteins upon differentiation at the non-permissive temperature. These results suggest that postmitotic neurons can be induced to enter the cell cycle without losing their commitment to a neuronal lineage.
journal_name
Brain Resjournal_title
Brain researchauthors
Eves EM,Kwon J,Downen M,Tucker MS,Wainer BH,Rosner MRdoi
10.1016/0006-8993(94)91484-2subject
Has Abstractpub_date
1994-09-12 00:00:00pages
396-404issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(94)91484-2journal_volume
656pub_type
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