Abstract：
:Leishmania spp. are the causative agents of visceral, cutaneous and mucocutaneous leishmaniosis with several taxa ("species") of the genus Leishmania being involved in human disease. As diagnostics based on microscopical detection of the parasites or on serological tests are often unsatisfactory, also molecular biological methods, particularly the polymerase chain reaction (PCR), have been employed for the detection of Leishmania spp. in the past years. The aim of the present study was to compare different PCR-protocols and optimise them for our needs, placing emphasis on the improvement of DNA isolation. PCR was performed with whole cell DNA isolated from cultures, as well as from simulated blood samples and clinical samples. Three different methods for the isolation of DNA from blood samples and two different PCR-protocols, one amplifying a fragment of the 18S rDNA and one for the amplification of the whole kDNA-circle, were applied and compared. No significant difference in sensitivity was detected between the different PCR-protocols, however, it was shown that the highest yield of DNA was achieved with a DNA isolation protocol based on urea.
journal_name
Wien Klin Wochenschrjournal_title
Wiener klinische Wochenschriftauthors
Fürnkranz U,Walochnik J,Grimm F,Deplazes P,Aspöck Hsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
30-4eissn
0043-5325issn
1613-7671journal_volume
116 Suppl 4pub_type
杂志文章abstract：:The first successful smallpox vaccination with cowpox lymph outside of England was carried out in Vienna--only ten months after the publication of Edward Jenner's book "An Inquiry into the Causes and Effects of the Variolae Vaccinae, a Disease ... known by the Name of the Cow Pox", and only a little more than three mo...
journal_title：Wiener klinische Wochenschrift
pub_type： 历史文章,杂志文章
doi：
更新日期：1999-04-23 00:00:00
abstract：:The inhibitory effect of somatostatin on the secretion of glucagon permits the examination of the effect of glucagon on some metabolic parameters in the insulin-dependent diabetic patient. The results obtained in 5 insulin-dependent diabetics and in 4 pancreatectomized patients revealed an inhibitory effect of somatos...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1976-09-03 00:00:00
abstract：:As a result of progress in the modern treatment of burns, systemic candidosis (CA), in the sense of "surviving mycoses", has become increasingly frequent. The site of entry is not usually the wound, but the intestinal tract, which becomes overgrown by the yeasts as a consequence of the requisite therapy with antibacte...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1976-11-12 00:00:00
abstract：:In a 29 year-old female occlusions of both femoral arteries were caused by the administration of ergotamine in the form of Cafergot-suppositories. Angiographically long smooth-walled stenoses and occlusions over a segment of approximately 5 cm length in each superficial femoral artery as well as occlusions of both tib...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1978-03-03 00:00:00
abstract：:Interpretation of lung function values in children with cystic fibrosis (CF) depends on the applied reference values. We hypothesize that differences between the new global lung function initiative (GLI) values and the formerly used Zapletal et al. values produce significantly different clinical results. We analyzed 3...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-017-1184-0
更新日期：2017-08-01 00:00:00
abstract：:The following parameters have been examined in twenty-one patients suffering from chronic renal failure (creatinine level between 4.5 and 18.8 mg/100 ml serum): maximum motor nerve conduction of the peroneal nerve, amplitude of the compound muscle action potential of the extensor digitorum brevis muscle, serum creatii...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1976-12-10 00:00:00
abstract：:Indirect mesentericoportography was performed in 36 patients after administration of prostaglandin E1 and immediately before contrast medium injection. The results were compared with the findings in a control group of 20 patients receiving tolazoline as vasodilator. On comparing prostaglandin E1 with tolazoline no sig...
journal_title：Wiener klinische Wochenschrift
pub_type： 临床试验,杂志文章
doi：
更新日期：1985-09-13 00:00:00
abstract：:Ulcerative colitis occurs before the age of 15 years in 20% and before 20 years in 40% of all patients involved. The frequency of this disease in patients at the University Children's Hospital of Munich has clearly risen over the past years. In many patients we found characteristic differences in history, clinical pic...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1986-08-29 00:00:00
abstract：:A qualitative cytological study demonstrated that normal cell count in the cerebrospinal fluid does not exclude abnormal cytological findings. Siderophages were found as a late indicator of haemorrhage into the CSF. In some cases of tumours of the CNS sporadic atypical cells were found without other alterations in CSF...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1981-06-12 00:00:00
abstract：:During the last decades a sharp increase in the occurrence of invasive aspergillosis associated with a high mortality has been observed. This fact is mainly due to an increase in the number of immunosuppressed patients. Nosocomial aspergillosis usually is of exogenous origin and often related to building construction ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章,评审
doi：
更新日期：1998-06-05 00:00:00
abstract：:The pharmacodynamic interaction between lumefantrine and its monodesbutyl analogue (DBB) has been investigated in 35 fresh isolates of Plasmodium falciparum. Both compounds showed highly significant activity correlation. The geometric mean values for complete inhibition of schizont maturation (GMCOC) were 536,8 nM for...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-008-1080-8
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to compare the surgical outcomes of patients operated on, with or without discography prior to operation. METHODS:The study was designed as a randomized controlled trial, using power analysis with McNemar's test on two correlated proportions. The study comprised of 310 patient...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00508-013-0404-5
更新日期：2013-10-01 00:00:00
abstract：:Peroxisomes were the latest subcellular organelles to be discovered. For a long time, due to limited knowledge of their function, peroxisomes were considered to play only a supporting role for other organelles in cellular metabolism. The discovery of specific metabolic pathways carried out exclusively in peroxisomes a...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:The principle of estimating the probability of monozygosity based on Bayes' formula is outlined for tweins with unknown parental genotypes. Tables based on Austrian gene frequencies are given for the common blood groups for easy computing of the chance of monozygosity. ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1980-01-18 00:00:00
abstract：:Hereditary fructose intolerance (HFI) was diagnosed in a 61 year-old male patient on account of liver dysfunction followed by prolonged shock immediately after the administration of a fructose and lactose infusion postoperatively. The diagnosis of HFI was based on an increased value of fructose, hypoglycaemia, lactic ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1978-03-31 00:00:00
abstract：:The Poison Control Centre in Vienna registered 4018 inquires regarding possible poisoning in children over a two-year period. The peak incidence occurred in the age group between 2 and 3 years and poisoning chiefly took place in the mornings and in the afternoons, the most frequently ingested substances being househol...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1978-01-06 00:00:00
abstract：BACKGROUND:In this study we investigated the neurolinguistic and neuroimaging characteristics of post-stroke motor aphasia patients. The effects of acupuncture on cortex activation by using magnetic resonance imaging (MRI) in patients with motor aphasia were also studied. METHODS:In this study 43 patients with motor a...
journal_title：Wiener klinische Wochenschrift
pub_type： 临床试验,杂志文章
doi：10.1007/s00508-016-1070-1
更新日期：2017-02-01 00:00:00
abstract：:The value of plate thermography in the determination of placental localization was investigated in 120 cases, and compared with the results obtained by the ultrasonic B-Scan method. The duration of pregnancy in these cases ranged from 15 to 37 weeks. The results of plate thermography corresponded with ultrasonic local...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1975-11-14 00:00:00
abstract：BACKGROUND:Rising costs of pharmaceuticals are a challenge to the public health care system. In collaboration with a company health insurance with 3143 members we analysed the economic benefit of reduced prescription fees for generic drugs in a 12-month period. METHODS:Within the observation period 1 euro per prescrip...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-007-0890-4
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVE:Our aim was to evaluate the usefulness of screening for cardiovascular risk factors and the effect of applying professional guidelines for risk reduction in postmenopausal women, as judged by the Framingham and the high-risk systematic coronary risk evaluation (SCORE) methods. METHODS:18 menopause clinics in...
journal_title：Wiener klinische Wochenschrift
pub_type： 临床试验,杂志文章
doi：10.1007/s00508-009-1152-4
更新日期：2009-01-01 00:00:00
abstract：:A report is given of 135 panendoscopic examinations in acute upper gastrointestinal bleeding. The importance of endoscopy is underlined by the high incidence of erosions and potential bleeding sources, since these lesions are not detected by radiological examination. Nor is it possible by means of X-rays to distinguis...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1976-10-15 00:00:00
abstract：:The ambient ozone concentration has increased greatly over the past decades, which may lead to environmental health problems, especially during the summer. WHO guidelines recommend an 8 hour mean value of < or = 60 ppb ozone without health risk. The aim of the present study was to investigate if elevated zone concentr...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1997-05-09 00:00:00
abstract：:Spermatological examinations were performed on 61 patients before and after varicocelectomy. Sperm morphology remained unchanged, but the sperm density increased in 67.2% cases and in 54.1% the sperm motility rose over a period of up to 18 months following the treatment. In 72.1% of the cases an improvement in at leas...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1982-04-30 00:00:00
abstract：:The value of cooperation between the paediatrician and the dentist in the field of nutrition and dietary instruction to maintain the health of children's dentition is stressed. The influence of the diet on the development of the dentition and the role of carbohydrates in the production of caries and parodontic disease...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1975-04-18 00:00:00
abstract：PURPOSE:This study aims to provide empirical data on physicians' intentions in withholding and withdrawing treatment, and to discuss possible implications for the ethical debate. BASIC PROCEDURES:The data presented come from EURELD, a large research project designed to investigate medical end-of-life decisions in six ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-006-0583-4
更新日期：2006-06-01 00:00:00
abstract：:Acute pandysautonomia is a rare disease defined as acute widespread and severe sympathetic and parasympathetic failure and sparing of somatic nerve fibers. The causes of this syndrome are often an autoimmune disease leading to autonomic ganglionopathy. The majority of cases have a poor prognosis with a chronic debilit...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-011-0030-z
更新日期：2011-08-01 00:00:00
abstract：:A preliminary report is presented on oral vaccination against whooping cough. 23 babies younger than, and 2 older than nine weeks and 12 newborn infants were vaccinated orally. No side effects were observed. Serum antibody titres did not increase markedly, but no child was seronegative after vaccination. An evident an...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1979-11-09 00:00:00
abstract：:To obtain a clearer definition of the influence of cardiac disease on the development of cerebrovascular disease (CVD), we carried out a comparative study of two groups of patients, matched for age and sex, one with and one without CVD. These were compared with regard to the incidence of cardiac disease, hypertension ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1981-06-12 00:00:00
abstract：:In the introduction, the changes in the clinical features of tuberculosis over the past few decades are analyzed. Despite the reverse trend in incidence, tuberculosis is still one of the major infectious diseases in the world. The essential points in fighting tuberculosis are prophylaxis and early diagnosis, therapy h...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Increased activity of the sympathetic nervous system plays a major role in the pathophysiology of primary arterial hypertension. Interventional renal sympathetic denervation (RSD) is a novel percutaneous treatment, decreasing sympathetic activity and consecutively blood pressure by ablating sympathetic nervous fibers ...
journal_title：Wiener klinische Wochenschrift
pub_type： 杂志文章
doi：10.1007/s00508-012-0257-3
更新日期：2012-11-01 00:00:00
