Abstract:
:Studies have shown that argyrophilic nucleolar organizer region-associated proteins (AgNORs) may correlate with DNA ploidy and/or proliferative activity in neoplastic and non-neoplastic conditions. However, studies have estimated only the mean AgNOR counts. Here we used two AgNOR counts, one of which may correlate with DNA ploidy and the other with proliferative activity. The mean AgNOR count (mAgNOR) was defined as the mean number of AgNORs/nucleus in 100 cells and may represent DNA or RNA index. The percentage of nuclei exhibiting 5 or more AgNORs/nucleus (pAgNOR) may reflect proliferative activity. These two AgNOR counts were correlated with results from acridine orange flow cytometry in 50 fine-needle aspirate (FNA) smears of nodal and extranodal sites, including three cases of reactive lymphadenopathy and 47 cases of non-Hodgkin's lymphoma. The mean mAgNOR count in the diploid specimens was 2.03 (+/- 0.74 SD) and 2.62 (+/- 0.73 SD) in the aneuploid tumors (P less than 0.0001). Samples with a low RNA index had mean mAgNOR of 1.80 (+/- 0.41 SD), whereas those with high RNA had a mean mAgNOR of 2.93 (+/- 0.86 SD) (P less than 0.0001). Lesions with low proliferative index, determined by flow cytometry, had a mean pAgNOR of 4%, whereas those with intermediate and high proliferative indices had a mean pAgNOR of 16% (P less than 0.0001). A similar but less significant correlation existed between RI and pAgNOR (P less than 0.005). We conclude that the two AgNOR counting methods may reliably reflect cell kinetics and distinguish ploidy from proliferative activity, making them useful adjuncts to flow cytometry in limited cytology specimens and small biopsy samples.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Mourad WA,Katz RL,Sembera D,Atkinson EN,el-Naggar AKdoi
10.1002/dc.2840080208subject
Has Abstractpub_date
1992-01-01 00:00:00pages
128-34issue
2eissn
8755-1039issn
1097-0339journal_volume
8pub_type
杂志文章abstract::This report details the cytologic features of primary localized amyloid tumor of the breast presenting as bilateral breast masses in a 72-yr-old woman. Clinically and radiographically, the masses simulated metastatic or multifocal carcinoma. Fine-needle aspiration revealed irregular globules of acellular amorphous mat...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840090521
更新日期:1993-10-01 00:00:00
abstract::Adenomatous neuroendocrine tumors of the middle ear (MEANTs) are very rare neoplasms which are composed of both epithelial and neuroendocrine components. They have mostly bland histology and indolent biological behavior, yet they may recur and metastasize. Here we present a case of MEANT with ipsilateral local brain, ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24640
更新日期:2020-10-12 00:00:00
abstract::The incidence of pancreatic adenocarcinoma is increasing and it is usually unresectable at the time of diagnosis. Consequently, fine-needle aspiration biopsy (FNAB) is being used more frequently for diagnosis. The reported sensitivity of diagnosing pancreatic adenocarcinoma by FNAB has varied between 50% and 100%. In ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840070404
更新日期:1991-01-01 00:00:00
abstract::Ameloblastoma is an odontogenic tumor with aggressive biological behavior, high recurrence rate, and a complex microscopic appearance with many different histologic patterns. Primary ameloblastoma is also described in extragnathic locations. Because of its wide morphologic spectrum, which is mirrored also in cytologic...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21823
更新日期:2013-03-01 00:00:00
abstract::A 53-yr-old woman with a 13-mo history of recurrent ovarian papillary serous adenocarcinoma presented with persistent microscopic hematuria. The patient was undergoing chemotherapy for her recurrent ovarian tumor when she was referred to the urology service for microscopic hematuria. An intravenous pyelogram was norma...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/(sici)1097-0339(199903)20:3<156::aid-dc8>3
更新日期:1999-03-01 00:00:00
abstract::Nephroblastoma (Wilms' tumor) is the most common childhood renal tumor and usually presents with a histology and cytology consisting of blastemal, epithelial, and stromal cells. Effusions are not uncommon and may suggest an unfavorable prognosis when containing anaplastic tumor cells. In the present case, we report th...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20535
更新日期:2006-09-01 00:00:00
abstract::Although fine-needle aspiration (FNA) cytologic features of conventional papillary thyroid carcinoma (PTC) and some of its variants have been documented in the literature, PTC with an adenoid cystic pattern has not so far been described. A 35-year-old woman presented with solitary cold nodule in the right lobe of thyr...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20063
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:We previously found that the presence of atypical urothelial tissue fragments (AUTF) was associated with an increased risk of high-grade urothelial carcinoma (HGUC) but not low-grade urothelial neoplasia (LGUN) in voided urine (VU) specimens. However, we subsequently found that patients with LGUN were more l...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23767
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:The number of "renal incidentalomas" is on the rise due to increasing use of radiologic studies. Image-guided core needle biopsies (CNB) with touch preparations are performed to guide specimen collection and triage of sample for additional studies. Results allow the clinical team to make appropriate treatmen...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24253
更新日期:2019-10-01 00:00:00
abstract::The role of fine-needle aspiration cytology (FNAC) in the diagnosis of benign skin lesions has been restricted primarily to the evaluation of bacteriologic and morphologic indices in leprosy. This study was undertaken to evaluate the efficacy of FNAC in the diagnosis and classification of lepromatous lesions. Aspirate...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.1068
更新日期:2001-05-01 00:00:00
abstract::The National Cancer Institute (NCI) sponsored the NCI Thyroid Fine Needle Aspiration (FNA) State of the Science Conference on October 22-23, 2007 in Bethesda, MD. The 2-day meeting was accompanied by a permanent informational Web site and several on-line discussion periods between May 1 and December 15, 2007 (http://t...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20832
更新日期:2008-06-01 00:00:00
abstract::Metastatic dissemination is the primary cause of death in ovarian cancer (OvCa) patients, and dissemination to pleural and peritoneal effusions is a common clinical event. Effusion samples were collected from 15 OvCa patients. Twenty-six samples were collected prospectively, two were archival, and eight were taken fro...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20279
更新日期:2005-11-01 00:00:00
abstract::While metastatic tumors to bone or lymph nodes from previously known primaries are often successfully diagnosed via fine-needle aspiration (FNA), a metastatic deposit in a patient with no previously known cancer may pose a diagnostic dilemma. Here, we present a case of metastatic papillary thyroid carcinoma that prese...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23031
更新日期:2014-08-01 00:00:00
abstract::The rate of positive cultures in fine-needle aspiration biopsy (FNAB) specimens is evaluated, and the value of submitting FNAB culture is assessed. Review of 3,300 FNAB specimens from 2,416 patients were tabulated for culture results, when obtained from the FNAB material. For positive culture results, clinical impact ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20648
更新日期:2008-02-01 00:00:00
abstract::Malignant mesothelioma is a rare neoplasm with poor prognosis. The pleural form is defined as a malignant tumor of mesothelial cells with a diffuse growth pattern involving the visceral and parietal surfaces of the pleura. To our knowledge, there have only been two reported cases in the literature where fine-needle as...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20314
更新日期:2005-08-01 00:00:00
abstract::Carcinoma of the lungs remains one of the primary causes of cancer mortality in the United States and represents a significant diagnostic challenge. Current diagnostic protocols depend substantially on cytology as an initial diagnostic modality. Pulmonary cytology can be diagnostically challenging with false positive ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.22858
更新日期:2012-06-01 00:00:00
abstract::Juvenile xanthogranuloma (JXG) is a rare lesion of the skin and deep tissues whose cytologic features have not previously been published. We report on 2 cases of JXG in patients aged 5 days and 5 yr who presented with an axillary mass and enlarged supraclavicular lymph node, respectively. Fine-needle aspiration and to...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0339(199611)15:4<329::AID-DC15>
更新日期:1996-11-01 00:00:00
abstract::In the past 10 years, 7,495 cytological breast fine-needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative res...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840030206
更新日期:1987-06-01 00:00:00
abstract::Pleomorphic lipoma is a rare soft-tissue tumor, most commonly seen in the head and neck regions of middle-aged men. Fine-needle aspiration (FNA) of these lesions can present a diagnostic challenge. Its large, hyperchromatic cells and multinucleated forms (floret cells) can easily be mistaken for a malignancy. A patien...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20183
更新日期:2005-02-01 00:00:00
abstract::Ovarian gonadoblastoma coexisting with a dysgerminoma is extremely rare in patients with Turner syndrome (TS) and a Y chromosome. The cytological findings, including imprint cytology, of these unusual ovarian tumors have rarely been reported. We report a rare patient with a gonadoblastoma with dysgerminoma, 3.0 × 2.0 ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.24282
更新日期:2019-11-01 00:00:00
abstract::Adenoviruses are emerging as important viral pathogens in immunocompromised patients due to immunodeficiency diseases and recently hematopoietic stem cell and solid organ transplant recipients, impacting morbidity and even mortality. Immunocompromised children are prone to respiratory infection, due to alterations in ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24662
更新日期:2020-11-16 00:00:00
abstract::The diagnosis of nodular sclerosing classical Hodgkin lymphoma (NSCHL) by fine-needle aspiration (FNA) biopsy has historically been a diagnostic challenge due to the usual paucicellularity of the specimen. This case report, and other previously published reports, suggests that there is another facet to the potentially...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21829
更新日期:2014-03-01 00:00:00
abstract::The purpose of this study was to assess whether the same principles to evaluate renal transplant by fine-needle aspiration (FNA) for rejection could be applied to pancreatic allograft. Between 1996-1998, 25 ultrasound-guided FNAs on 13 patients with pancreatic allograft were performed and ThinPrep made. The percentage...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10179
更新日期:2002-11-01 00:00:00
abstract::Histoplasmosis not uncommonly causes systemic infection, particularly in immunocompromised patients. In systemic infection, the urinary tract is often involved, although the diagnosis of histoplasmosis in urine cytologic specimens has never been reported. Urinary tract histoplasmosis may present with gross hematuria, ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10049
更新日期:2002-04-01 00:00:00
abstract::We present the cytologic features and the immunocytochemical profile of four cases of chordoma on fine-needle aspiration biopsies. The physaliferous cells in signet-ring, pearl-like formations and the trabecular arrangement with rounded contours are distinctive. Other cell types and cellular arrangements are also desc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020112
更新日期:1986-01-01 00:00:00
abstract::Acinar cell carcinoma (ACC) and islet cell tumor (ICT), both rare pancreatic neoplasms, can be diagnosed accurately and rapidly with the use of imaging-guided fine-needle aspiration biopsies. The specific cytologic features of these tumors are described in a series of 17 patients, and histologic and immunocytochemical...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199702)16:2<112::aid-dc3>3
更新日期:1997-02-01 00:00:00
abstract::Pseudovascular adenoid squamous-cell carcinoma (PASCC) is an epithelial malignancy with spindle-cell features that can mimic vascular neoplasms, particularly angiosarcoma, in its morphologic characteristics. There have been only a few surgical pathology reports of PASCC arising in the lung, skin, and breast. We descri...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199910)21:4<265::aid-dc7>3
更新日期:1999-10-01 00:00:00
abstract::Anaplastic large-cell lymphoma (ALCL) is a rare T-cell lymphoma typically seen in children and young adults. It has been described in numerous sites; however, the breast is one of the least common locations. We herein report a case of ALCL arising in the breast of a 36-yr-old pregnant woman. To our knowledge this is t...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20442
更新日期:2006-04-01 00:00:00
abstract::Very rarely lymphoma primarily or secondarily involves the pancreas. Involvement of the pancreatic parenchyma with lymphoma clinically may mimic pancreatic ductal adenocarcinoma (PDA) and other mass-forming pancreatic lesions. Endoscopic ultrasound fine needle aspiration (EUS-FNA) is the first step in the diagnostic p...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24349
更新日期:2020-04-01 00:00:00
abstract::Mixed adenoneuroendocrine carcinoma (MANEC) is defined as a tumor that has morphologically recognizable both adenocarcinoma and neuroendocrine carcinoma components comprising at least 30% of either components. MANEC occurring in the ampulla of Vater is extremely rare, and only 16 cases have been reported in the Englis...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23891
更新日期:2018-06-01 00:00:00