Two AgNOR counts in fine-needle aspirates of lymphoproliferative disorders compared with acridine orange flow cytometry.

Abstract:

:Studies have shown that argyrophilic nucleolar organizer region-associated proteins (AgNORs) may correlate with DNA ploidy and/or proliferative activity in neoplastic and non-neoplastic conditions. However, studies have estimated only the mean AgNOR counts. Here we used two AgNOR counts, one of which may correlate with DNA ploidy and the other with proliferative activity. The mean AgNOR count (mAgNOR) was defined as the mean number of AgNORs/nucleus in 100 cells and may represent DNA or RNA index. The percentage of nuclei exhibiting 5 or more AgNORs/nucleus (pAgNOR) may reflect proliferative activity. These two AgNOR counts were correlated with results from acridine orange flow cytometry in 50 fine-needle aspirate (FNA) smears of nodal and extranodal sites, including three cases of reactive lymphadenopathy and 47 cases of non-Hodgkin's lymphoma. The mean mAgNOR count in the diploid specimens was 2.03 (+/- 0.74 SD) and 2.62 (+/- 0.73 SD) in the aneuploid tumors (P less than 0.0001). Samples with a low RNA index had mean mAgNOR of 1.80 (+/- 0.41 SD), whereas those with high RNA had a mean mAgNOR of 2.93 (+/- 0.86 SD) (P less than 0.0001). Lesions with low proliferative index, determined by flow cytometry, had a mean pAgNOR of 4%, whereas those with intermediate and high proliferative indices had a mean pAgNOR of 16% (P less than 0.0001). A similar but less significant correlation existed between RI and pAgNOR (P less than 0.005). We conclude that the two AgNOR counting methods may reliably reflect cell kinetics and distinguish ploidy from proliferative activity, making them useful adjuncts to flow cytometry in limited cytology specimens and small biopsy samples.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Mourad WA,Katz RL,Sembera D,Atkinson EN,el-Naggar AK

doi

10.1002/dc.2840080208

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

128-34

issue

2

eissn

8755-1039

issn

1097-0339

journal_volume

8

pub_type

杂志文章
  • Localized primary amyloid tumor associated with osseous metaplasia presenting as bilateral breast masses: cytologic and radiologic features.

    abstract::This report details the cytologic features of primary localized amyloid tumor of the breast presenting as bilateral breast masses in a 72-yr-old woman. Clinically and radiographically, the masses simulated metastatic or multifocal carcinoma. Fine-needle aspiration revealed irregular globules of acellular amorphous mat...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840090521

    authors: Lynch LA,Moriarty AT

    更新日期:1993-10-01 00:00:00

  • Adenomatous neuroendocrine tumors of the middle ear (MEANTs) with local invasion and lymph nodes metastasis: A brief report of a rare neoplasm.

    abstract::Adenomatous neuroendocrine tumors of the middle ear (MEANTs) are very rare neoplasms which are composed of both epithelial and neuroendocrine components. They have mostly bland histology and indolent biological behavior, yet they may recur and metastasize. Here we present a case of MEANT with ipsilateral local brain, ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24640

    authors: Yang L,Asiry S,Khader SN

    更新日期:2020-10-12 00:00:00

  • Pancreatic adenocarcinoma: regression analysis to identify improved cytologic criteria.

    abstract::The incidence of pancreatic adenocarcinoma is increasing and it is usually unresectable at the time of diagnosis. Consequently, fine-needle aspiration biopsy (FNAB) is being used more frequently for diagnosis. The reported sensitivity of diagnosing pancreatic adenocarcinoma by FNAB has varied between 50% and 100%. In ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070404

    authors: Cohen MB,Egerter DP,Holly EA,Ahn DK,Miller TR

    更新日期:1991-01-01 00:00:00

  • Fine-needle aspiration cytology of ameloblastoma and malignant ameloblastoma: a study of 12 cases.

    abstract::Ameloblastoma is an odontogenic tumor with aggressive biological behavior, high recurrence rate, and a complex microscopic appearance with many different histologic patterns. Primary ameloblastoma is also described in extragnathic locations. Because of its wide morphologic spectrum, which is mirrored also in cytologic...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21823

    authors: Klapsinou E,Stavros A,Smaragda A,Despoina P,Dimitra D

    更新日期:2013-03-01 00:00:00

  • Cytologic diagnosis of metastatic ovarian adenocarcinoma in the urinary bladder: a case report and review of the literature.

    abstract::A 53-yr-old woman with a 13-mo history of recurrent ovarian papillary serous adenocarcinoma presented with persistent microscopic hematuria. The patient was undergoing chemotherapy for her recurrent ovarian tumor when she was referred to the urology service for microscopic hematuria. An intravenous pyelogram was norma...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1097-0339(199903)20:3<156::aid-dc8>3

    authors: Edgerton ME,Hoda RS,Gupta PK

    更新日期:1999-03-01 00:00:00

  • Cytological identification of metastatic epithelial nephroblastoma in pleural fluid: report of a case and review of literature.

    abstract::Nephroblastoma (Wilms' tumor) is the most common childhood renal tumor and usually presents with a histology and cytology consisting of blastemal, epithelial, and stromal cells. Effusions are not uncommon and may suggest an unfavorable prognosis when containing anaplastic tumor cells. In the present case, we report th...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20535

    authors: Schinstine M,Abati A,Tsokos M,Fox E,Filie AC

    更新日期:2006-09-01 00:00:00

  • Papillary thyroid carcinoma with an adenoid cystic pattern: report of a case with fine-needle aspiration cytology and immunocytochemistry.

    abstract::Although fine-needle aspiration (FNA) cytologic features of conventional papillary thyroid carcinoma (PTC) and some of its variants have been documented in the literature, PTC with an adenoid cystic pattern has not so far been described. A 35-year-old woman presented with solitary cold nodule in the right lobe of thyr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20063

    authors: Haji BE,Ahmed MS,Prasad A,Omar MS,Das DK

    更新日期:2004-06-01 00:00:00

  • Urinary tract washing specimens containing atypical urothelial tissue fragments are significantly associated with urothelial neoplasia.

    abstract:BACKGROUND:We previously found that the presence of atypical urothelial tissue fragments (AUTF) was associated with an increased risk of high-grade urothelial carcinoma (HGUC) but not low-grade urothelial neoplasia (LGUN) in voided urine (VU) specimens. However, we subsequently found that patients with LGUN were more l...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23767

    authors: Zhang ML,Zhou AG,Rosenthal DL,VandenBussche CJ

    更新日期:2017-09-01 00:00:00

  • Improving ROSE: Discrepant touch preparation and histology findings in cytology of renal masses: A 10-year retrospective review.

    abstract:BACKGROUND:The number of "renal incidentalomas" is on the rise due to increasing use of radiologic studies. Image-guided core needle biopsies (CNB) with touch preparations are performed to guide specimen collection and triage of sample for additional studies. Results allow the clinical team to make appropriate treatmen...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24253

    authors: Shachner TR,Nodit L,Hubbard E,Van Meter S

    更新日期:2019-10-01 00:00:00

  • Utility of fine-needle aspiration cytology in the classification of leprosy.

    abstract::The role of fine-needle aspiration cytology (FNAC) in the diagnosis of benign skin lesions has been restricted primarily to the evaluation of bacteriologic and morphologic indices in leprosy. This study was undertaken to evaluate the efficacy of FNAC in the diagnosis and classification of lepromatous lesions. Aspirate...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.1068

    authors: Rao IS,Singh MK,Gupta SD,Pandhi RK,Kapila K

    更新日期:2001-05-01 00:00:00

  • Post-thyroid FNA testing and treatment options: a synopsis of the National Cancer Institute Thyroid Fine Needle Aspiration State of the Science Conference.

    abstract::The National Cancer Institute (NCI) sponsored the NCI Thyroid Fine Needle Aspiration (FNA) State of the Science Conference on October 22-23, 2007 in Bethesda, MD. The 2-day meeting was accompanied by a permanent informational Web site and several on-line discussion periods between May 1 and December 15, 2007 (http://t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20832

    authors: Layfield LJ,Abrams J,Cochand-Priollet B,Evans D,Gharib H,Greenspan F,Henry M,LiVolsi V,Merino M,Michael CW,Wang H,Wells SA

    更新日期:2008-06-01 00:00:00

  • Malignant effusions are sources of fibronectin and other promigratory and proinvasive components.

    abstract::Metastatic dissemination is the primary cause of death in ovarian cancer (OvCa) patients, and dissemination to pleural and peritoneal effusions is a common clinical event. Effusion samples were collected from 15 OvCa patients. Twenty-six samples were collected prospectively, two were archival, and eight were taken fro...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20279

    authors: Kohn EC,Travers LA,Kassis J,Broome U,Klominek J

    更新日期:2005-11-01 00:00:00

  • FNA diagnosis of a metastatic papillary thyroid carcinoma arising from a previously unknown follicular variant of papillary thyroid microcarcinoma.

    abstract::While metastatic tumors to bone or lymph nodes from previously known primaries are often successfully diagnosed via fine-needle aspiration (FNA), a metastatic deposit in a patient with no previously known cancer may pose a diagnostic dilemma. Here, we present a case of metastatic papillary thyroid carcinoma that prese...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23031

    authors: Jedrzkiewicz J,Witt BL

    更新日期:2014-08-01 00:00:00

  • Clinical significance of cultures collected from fine-needle aspiration biopsy.

    abstract::The rate of positive cultures in fine-needle aspiration biopsy (FNAB) specimens is evaluated, and the value of submitting FNAB culture is assessed. Review of 3,300 FNAB specimens from 2,416 patients were tabulated for culture results, when obtained from the FNAB material. For positive culture results, clinical impact ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20648

    authors: Granville LA,Laucirica R,Verstovsek G

    更新日期:2008-02-01 00:00:00

  • Primary diagnosis of malignant mesothelioma by fine-needle aspiration of a supraclavicular lymph node.

    abstract::Malignant mesothelioma is a rare neoplasm with poor prognosis. The pleural form is defined as a malignant tumor of mesothelial cells with a diffuse growth pattern involving the visceral and parietal surfaces of the pleura. To our knowledge, there have only been two reported cases in the literature where fine-needle as...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20314

    authors: Tafazzoli A,Raza A,Martin SE

    更新日期:2005-08-01 00:00:00

  • Respiratory cytology in the era of molecular diagnostics: a review.

    abstract::Carcinoma of the lungs remains one of the primary causes of cancer mortality in the United States and represents a significant diagnostic challenge. Current diagnostic protocols depend substantially on cytology as an initial diagnostic modality. Pulmonary cytology can be diagnostically challenging with false positive ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.22858

    authors: Witt BL,Wallander ML,Layfield LJ,Hirschowitz S

    更新日期:2012-06-01 00:00:00

  • Cytologic features of deep juvenile xanthogranuloma.

    abstract::Juvenile xanthogranuloma (JXG) is a rare lesion of the skin and deep tissues whose cytologic features have not previously been published. We report on 2 cases of JXG in patients aged 5 days and 5 yr who presented with an axillary mass and enlarged supraclavicular lymph node, respectively. Fine-needle aspiration and to...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0339(199611)15:4<329::AID-DC15>

    authors: Grenko RT,Sickel JZ,Abendroth CS,Cilley RE

    更新日期:1996-11-01 00:00:00

  • Fine-needle aspiration of breast lesions: role and accuracy in a review of 7,495 cases.

    abstract::In the past 10 years, 7,495 cytological breast fine-needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative res...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840030206

    authors: Fessia L,Botta G,Arisio R,Verga M,Aimone V

    更新日期:1987-06-01 00:00:00

  • Fine-needle aspiration of a pleomorphic lipoma of the head and neck: a case report.

    abstract::Pleomorphic lipoma is a rare soft-tissue tumor, most commonly seen in the head and neck regions of middle-aged men. Fine-needle aspiration (FNA) of these lesions can present a diagnostic challenge. Its large, hyperchromatic cells and multinucleated forms (floret cells) can easily be mistaken for a malignancy. A patien...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20183

    authors: Yong M,Raza AS,Greaves TS,Cobb CJ

    更新日期:2005-02-01 00:00:00

  • Usefulness of imprint cytology of gonadoblastoma with dysgerminoma in a patient with Turner syndrome and a Y chromosome: A case report and literature review.

    abstract::Ovarian gonadoblastoma coexisting with a dysgerminoma is extremely rare in patients with Turner syndrome (TS) and a Y chromosome. The cytological findings, including imprint cytology, of these unusual ovarian tumors have rarely been reported. We report a rare patient with a gonadoblastoma with dysgerminoma, 3.0 × 2.0 ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.24282

    authors: Nagai T,Hasegawa K,Motegi E,Machida H,Sasaki H,Kato H,Kosaka N,Fukasawa I,Kuroda H

    更新日期:2019-11-01 00:00:00

  • Adenoviral cytopathic changes in pleural fluid: A unique cytological presentation mimicking malignancy.

    abstract::Adenoviruses are emerging as important viral pathogens in immunocompromised patients due to immunodeficiency diseases and recently hematopoietic stem cell and solid organ transplant recipients, impacting morbidity and even mortality. Immunocompromised children are prone to respiratory infection, due to alterations in ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24662

    authors: Harbhajanka A,Michael CW

    更新日期:2020-11-16 00:00:00

  • Nodular sclerosing classical Hodgkin lymphoma masquerading as acute suppurative-necrotizing lymphadenitis.

    abstract::The diagnosis of nodular sclerosing classical Hodgkin lymphoma (NSCHL) by fine-needle aspiration (FNA) biopsy has historically been a diagnostic challenge due to the usual paucicellularity of the specimen. This case report, and other previously published reports, suggests that there is another facet to the potentially...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21829

    authors: Florentine BD,Cohen AN

    更新日期:2014-03-01 00:00:00

  • Diagnostic role of fine-needle aspiration of pancreatic allograft to detect rejection.

    abstract::The purpose of this study was to assess whether the same principles to evaluate renal transplant by fine-needle aspiration (FNA) for rejection could be applied to pancreatic allograft. Between 1996-1998, 25 ultrasound-guided FNAs on 13 patients with pancreatic allograft were performed and ThinPrep made. The percentage...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10179

    authors: Sariya D,Kluskens L,Assad L,Treaba D,Reddy V,Gattuso P

    更新日期:2002-11-01 00:00:00

  • Diagnosis of histoplasmosis in urine cytology: reactive urothelial changes, a diagnostic pitfall. Case report and literature review of urinary tract infections.

    abstract::Histoplasmosis not uncommonly causes systemic infection, particularly in immunocompromised patients. In systemic infection, the urinary tract is often involved, although the diagnosis of histoplasmosis in urine cytologic specimens has never been reported. Urinary tract histoplasmosis may present with gross hematuria, ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10049

    authors: Mukunyadzi P,Johnson M,Wyble JG,Scott M

    更新日期:2002-04-01 00:00:00

  • Chordoma: cytologic and immunocytochemical study of four cases.

    abstract::We present the cytologic features and the immunocytochemical profile of four cases of chordoma on fine-needle aspiration biopsies. The physaliferous cells in signet-ring, pearl-like formations and the trabecular arrangement with rounded contours are distinctive. Other cell types and cellular arrangements are also desc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840020112

    authors: Kontozoglou T,Qizilbash AH,Sianos J,Stead R

    更新日期:1986-01-01 00:00:00

  • Comparative cytologic features of pancreatic acinar cell carcinoma and islet cell tumor.

    abstract::Acinar cell carcinoma (ACC) and islet cell tumor (ICT), both rare pancreatic neoplasms, can be diagnosed accurately and rapidly with the use of imaging-guided fine-needle aspiration biopsies. The specific cytologic features of these tumors are described in a series of 17 patients, and histologic and immunocytochemical...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199702)16:2<112::aid-dc3>3

    authors: Labate AM,Klimstra DL,Zakowski MF

    更新日期:1997-02-01 00:00:00

  • Fine-needle aspiration cytologic features of pseudovascular adenoid squamous-cell carcinoma of the lung.

    abstract::Pseudovascular adenoid squamous-cell carcinoma (PASCC) is an epithelial malignancy with spindle-cell features that can mimic vascular neoplasms, particularly angiosarcoma, in its morphologic characteristics. There have been only a few surgical pathology reports of PASCC arising in the lung, skin, and breast. We descri...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199910)21:4<265::aid-dc7>3

    authors: Smith AR,Raab SS,Landreneau RJ,Silverman JF

    更新日期:1999-10-01 00:00:00

  • Pregnancy-associated anaplastic large-cell lymphoma of the breast: a rare mimic of ductal carcinoma.

    abstract::Anaplastic large-cell lymphoma (ALCL) is a rare T-cell lymphoma typically seen in children and young adults. It has been described in numerous sites; however, the breast is one of the least common locations. We herein report a case of ALCL arising in the breast of a 36-yr-old pregnant woman. To our knowledge this is t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20442

    authors: Iyengar P,Reid-Nicholson M,Moreira AL

    更新日期:2006-04-01 00:00:00

  • Pancreatic lymphoma: A cytologic diagnosis challenge.

    abstract::Very rarely lymphoma primarily or secondarily involves the pancreas. Involvement of the pancreatic parenchyma with lymphoma clinically may mimic pancreatic ductal adenocarcinoma (PDA) and other mass-forming pancreatic lesions. Endoscopic ultrasound fine needle aspiration (EUS-FNA) is the first step in the diagnostic p...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24349

    authors: Savari O,Al-Duwal Z,Wang Z,Ganesan S,Danan-Rayes R,Ayub S

    更新日期:2020-04-01 00:00:00

  • Cytological features of mixed adenoneuroendocrine carcinoma of the ampulla of Vater: A case report with immunocytochemical analyses.

    abstract::Mixed adenoneuroendocrine carcinoma (MANEC) is defined as a tumor that has morphologically recognizable both adenocarcinoma and neuroendocrine carcinoma components comprising at least 30% of either components. MANEC occurring in the ampulla of Vater is extremely rare, and only 16 cases have been reported in the Englis...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23891

    authors: Yoshioka S,Ebisu Y,Ishida M,Uemura Y,Yanagimoto H,Satoi S,Tsuta K

    更新日期:2018-06-01 00:00:00