Abstract：
:The measurement of amino acid neurotransmitters by high-performance liquid chromatography (HPLC) has emerged as a reliable and sensitive method. This paper describes a method which employs electrochemical (EC) detection of amino acid derivatives formed by a reaction with o-phthalaldehyde (OPA) in the presence of sulphite ions. This is discussed in relation to the problems of previously reported methods based on OPA derivatisation. Precise separation of the following 7 amino acid standards is achieved using isocratic elution: serine, glycine, taurine, glutamate, arginine, alanine and GABA, in order of increasing retention time. Total elution time is 25 min. Derivatisation proceeds at room temperature and the derivatives are stable for up to 5 h. This technique has the sensitivity to determine the concentrations of amino acid neurotransmitters in cerebrospinal fluid (CSF) and an in vivo microdialysis method is discussed for the detection of basal and potassium-stimulated levels of gamma-aminobutyric acid and glutamate from rat hippocampus.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Rowley HL,Martin KF,Marsden CAdoi
10.1016/0165-0270(94)00132-zsubject
Has Abstractpub_date
1995-03-01 00:00:00pages
93-9issue
1eissn
0165-0270issn
1872-678Xpii
016502709400132Zjournal_volume
57pub_type
杂志文章abstract：:An intra-arterially perfused working heart-brainstem preparation (WH-BP) was developed to allow studies into functionally identified cardiovascular and respiratory neurones in an in vitro milieu. This report provides that first description of this preparation. Evidence is presented indicating that the WH-BP: (1) spont...
journal_title：Journal of neuroscience methods
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doi：10.1016/0165-0270(95)00147-6
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:Functional magnetic resonance imaging (fMRI) enables non-invasive examination of both the structure and the function of the human brain. The prevalence of high spatial-resolution (sub-millimeter) fMRI has triggered new research on the intra-cortex, such as cortical columns and cortical layers. At present, ec...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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更新日期：1998-12-31 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1999-07-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00207-w
更新日期：1995-07-01 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1995-11-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2018-01-01 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2020.108601
更新日期：2020-01-22 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90066-9
更新日期：1984-02-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2014-03-30 00:00:00
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2007.05.031
更新日期：2007-09-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.11.032
更新日期：2008-03-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.11.002
更新日期：2018-05-15 00:00:00
abstract：:Motor neurons innervating tiny muscles in the antennal system of the cockroach, Periplaneta americana, were stained by two new approaches to backfilling motor axons and somata. Both methods are easier, and produce less leakage of dye, than conventional methods that rely on application of dye to the distal cut end of a...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00203-x
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abstract：:The Visual Paired Comparison (VPC) task is a recognition memory test that has shown promise for the detection of memory impairments associated with mild cognitive impairment (MCI). Because patients with MCI often progress to Alzheimer's Disease (AD), the VPC may be useful in predicting the onset of AD. VPC uses noninv...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,随机对照试验
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更新日期：2011-09-30 00:00:00
