Abstract：
:Monitoring of BCR-ABL transcript level is widely used in chronic myeloid leukemia (CML) to follow up response to therapy. In this study we compare abilities of two quantitative RT-PCR assays to characterize the disease status in CML patients: RT-PCR quantifying the BCR-ABL transcript concentration and RT-PCR determining the BCR-ABL/BCR transcript ratio (R). We demonstrate that in non-responders only R, but not BCR-ABL, unambiguously characterizes the state of disease. Moreover, R values >1 found in all poor responders indicate lower BCR expression compared to BCR- ABL in these patients. Our results demonstrate the importance of BCR-ABL/BCR transcript ratio for the disease status and the disease prognosis characterization and suggest a possible role of the normal BCR gene expression in CML pathogenesis.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Moravcova J,Regner J,Mouckova D,Fiser K,Zmekova V,Malacova R,Michalova K,Klamova Hsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
119-25issue
2eissn
0028-2685issn
1338-4317journal_volume
52pub_type
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