Abstract:
:The original activated partial thromboplastin time-based assay for activated protein C (APC)-resistant factor Va (FVa) requires carefully prepared fresh plasma and cannot be used in patients receiving warfarin or in patients with antiphospholipid antibodies. A new test is described here that circumvents these limitations and distinguishes without overlap heterozygotes for APC-resistant FVa from persons with normal FV. A diluted test plasma is incubated with an FV-deficient substrate plasma and tissue factor and then clotted with Ca2+ or Ca2+ plus APC. Test results are independent of the FV level or the dilution of the test plasma used. Of 39 controls, 37 gave normal results. Two controls (5%) gave results indicative of APC resistant FVa and on DNA analysis were found to be heterozygous for FV R506Q. Twenty of 21 randomly selected patients receiving warfarin gave normal results. In the single patient with abnormal results, heterozygous FV R506Q was confirmed by DNA analysis. Two of 15 patients with protein S deficiency and 5 of 29 patients with a lupus anticoagulant had abnormal results. APC resistance caused by FV R506Q was confirmed in the five of these seven patients available for DNA analysis. APC-resistant FVa was also detected in 10 of 21 (46%) stored plasma from unrelated patients with venous thrombosis and negative earlier evaluation for a lupus anticoagulant or a deficiency of protein C, protein S, or antithrombin, which confirms a high incidence of this defect among patients with venous thrombosis.
journal_name
Bloodjournal_title
Bloodauthors
Le DT,Griffin JH,Greengard JS,Mujumdar V,Rapaport SIsubject
Has Abstractpub_date
1995-04-01 00:00:00pages
1704-11issue
7eissn
0006-4971issn
1528-0020journal_volume
85pub_type
杂志文章相关文献
BLOOD文献大全abstract::ADP-induced TXA2 generation requires the costimulation of P2Y1, P2Y12, and the GPIIb/IIIa receptors. Signaling events downstream of the P2Y receptors that contribute to ADP-induced TXA2 generation have not been clearly delineated. In this study, we have investigated the role of G-protein-gated inwardly rectifying pota...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-03-010330
更新日期:2006-11-01 00:00:00
abstract::The initiation of primary hemostasis is mediated by interaction of the platelet glycoprotein Ib (GPIb) surface receptor and its arterial subendothelial von Willebrand factor (vWF) ligand. The intracellular signaling immediately following GPIb receptor occupancy connecting the adhesive event to platelet activation and ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-02-15 00:00:00
abstract::The MLLT10 gene, located at 10p13, is a known partner of MLL and PICALM in specific leukemic fusions generated from recurrent 11q23 and 11q14 chromosome translocations. Deep sequencing recently identified NAP1L1/12q21 as another MLLT10 partner in T-cell acute lymphoblastic leukemia (T-ALL). In pediatric T-ALL, we have...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-02-487256
更新日期:2013-06-20 00:00:00
abstract::Professional antigen-presenting cells (APCs) are sentinel cells of the immune system that present antigen to T lymphocytes and mediate an appropriate immune response. It is therefore surprising that knowledge of the professional APCs in human lymph nodes is limited. Using 3-color immunohistochemistry, we have identifi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-06-165266
更新日期:2009-02-05 00:00:00
abstract::To assess the influence of graft-versus-host disease (GVHD) on recurrent leukemia and survival after allogeneic marrow transplantation, we studied 1,202 patients with acute nonlymphocytic leukemia (ANL), acute lymphocytic leukemia (ALL), and chronic myelogenous leukemia (CML) given unmodified marrow grafts from HLA-id...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Human mast cells in adult tissues have been thought to have limited, if any, proliferative potential. The current study examined mast cells obtained from adult skin and cultured in serum-free medium with recombinant human stem cell factor. During the first 4 weeks of culture, the percentages of mast cells increased fr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.7.2045
更新日期:2001-04-01 00:00:00
abstract::We report the nonrandom occurrence, frequency, and degree of immunophenotype association of the t(1;14)(p34;q11) in children with acute lymphoblastic leukemia (ALL). This chromosomal abnormality occurred in leukemia cells from 5 of 1,630 (0.3%) consecutive children with newly diagnosed ALL who were entered on a single...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-09-15 00:00:00
abstract::The presence and physiologic role of cyclic GMP-dependent protein kinase (G-kinase) in human neutrophils was investigated by Western blot analysis and immunocytochemistry. Small quantities of G-kinase were found in the cytoskeletal-enriched fraction of neutrophil lysates as detected by Western blots using a polyclonal...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::We have investigated the role of plasminogen activator inhibitor 1 (PAI-1) in the regulation of fibrinolysis using a model thrombus composed of thrombin-stimulated platelets, fibrin(ogen), plasminogen, and recombinant tissue-type plasminogen activator. Laser light scattering kinetic measurements showed that clot lysis...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Hemojuvelin (HJV) positively modulates the iron regulator hepcidin, and its mutations are the major cause of juvenile hemochromatosis (JH), a recessive disease leading to iron overload. Defective HJV reduces hepcidin up-regulation both in humans and in Hjv-deficient mice. To investigate the JH pathogenesis and the fun...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-08-041004
更新日期:2007-05-15 00:00:00
abstract::Blood cells of an adult vertebrate are continuously generated by hematopoietic stem cells (HSCs) that originate during embryonic life within the aorta-gonad-mesonephros region. There is now compelling in vivo evidence that HSCs are generated from aortic endothelial cells and that this process is critically regulated b...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-264382
更新日期:2010-08-12 00:00:00
abstract::We investigated the clinical activity of the farnesyl transferase inhibitor R115777 in 22 patients with chronic myelogenous leukemia (CML) in chronic, accelerated, or blastic phase and in 8 patients with myelofibrosis (MF) and 10 patients with multiple myeloma (MM). R115777 was administered at 600 mg orally twice dail...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2002-07-1973
更新日期:2003-03-01 00:00:00
abstract::We here investigate the occurrence of fluoride intake-associated alterations in patients with hematologic disease on triazol antifungal medication. Clinical, laboratory, and radiology data of overall 43 patients with hematologic malignancies taking voriconazole (n = 20), posaconazole (n = 8), and itraconazole (n = 4),...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-01-403030
更新日期:2012-09-20 00:00:00
abstract::Fetal hemoglobin (HbF) induction can ameliorate the clinical severity of sickle cell disease and β-thalassemia. We previously reported that activation of the eukaryotic initiation factor 2α (eIF2α) stress pathway increased HbF through a posttranscriptional mechanism. In this study, we explored the underlying means by ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-03-564302
更新日期:2014-10-23 00:00:00
abstract::We previously reported the characterization of human osteoclast-associated receptor (hOSCAR), a novel Fc receptor gamma-chain (FcRgamma)-associated receptor expressed by myeloid cells. Here we show that ligation of hOSCAR by specific antibodies promotes dendritic cell (DC) survival by an extracellular signal-regulated...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-07-2809
更新日期:2005-05-01 00:00:00
abstract::In patients with common variable immunodeficiency (CVI), we have previously defined a subgroup of patients (CVIHyper) characterized by decreased numbers of CD4+ lymphocytes in peripheral blood, splenomegaly, and persistent immune activation in vivo, particularly of monocytes/macrophages. To further characterize this h...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-01-15 00:00:00
abstract::AML1-ETO (AE) is a fusion product of t(8;21) observed in 40% French-American-British M2 type of acute myeloid leukemia (AML). Clinical data suggest that Ras mutation is a frequent cooperating event in t(8;21) AML. Whether constitutively active Ras promotes leukemogenesis on the t(8;21) background has not been demonstr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-264119
更新日期:2011-02-17 00:00:00
abstract::The underlying pathways that lead to relapse in childhood acute lymphoblastic leukemia (ALL) are unknown. To comprehensively characterize the molecular evolution of relapsed childhood B-precursor ALL, we used human 500K single-nucleotide polymorphism arrays to identify somatic copy number alterations (CNAs) in 20 diag...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-06-165027
更新日期:2008-11-15 00:00:00
abstract::The Epstein-Barr virus (EBV) is associated with distinct forms of human lymphoid malignancies, including the endemic (eBL) and sporadic forms of Burkitt's lymphoma (sBL) and acquired immunodeficiency syndrome-associated non-Hodgkin lymphoma (AIDS-NHL). However, whether EBV has a pathogenetic role in these tumors or is...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::Recent studies have shown a discrepancy between the level of tissue factor (TF) expression and the level of TF procoagulant activity on the apical and basolateral surface domains of polarized epithelial cells. The present investigation was performed to elucidate possible reasons for the discordant expression of TF and...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Cancer patients are at increased risk of deep vein thrombosis and pulmonary embolism. The incidence among different groups of cancer patients varies considerably depending on clinical factors, the most important being tumor entity and stage. Biomarkers have been specifically investigated for their capacity of predicti...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2013-04-460147
更新日期:2013-09-19 00:00:00
abstract::Reprogramming of somatic cells to desired cell types holds great promise in regenerative medicine. However, production of transplantable hematopoietic stem cells (HSCs) in vitro by defined factors has not yet been achieved. Therefore, it is critical to fully understand the molecular mechanisms of HSC development in vi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-10-462655
更新日期:2013-07-18 00:00:00
abstract::We describe the development of acute myeloid leukemia (AML) in an adult with CBL syndrome caused by a heterozygous de novo germline mutation in CBL codon D390. In the AML bone marrow, the mutated CBL allele was homozygous after copy number-neutral loss-of-heterozygosity and amplified through a chromosomal gain; moreov...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-533844
更新日期:2014-03-20 00:00:00
abstract::HLA-G is a major histocompatibility complex class Ib molecule whose constitutive tissue distribution is restricted mainly to trophoblast cells at the maternal-fetal interface during pregnancy. In this study, we demonstrated the ability of the soluble HLA-G1 (sHLA-G1) isoform to inhibit fibroblast growth factor-2 (FGF2...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-12-019919
更新日期:2006-10-15 00:00:00
abstract::Multinucleated giant cells (MGC) are a common feature of granulomas that develop during various inflammatory reactions. MGC originate from fusion of monocytes or macrophages, but the exact mechanism of their generation is still unclear. In the present study, we investigated the influence of monocyte to macrophage matu...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-01-15 00:00:00
abstract::Umbilical cord blood (UCB) is an attractive cell source for hematopoietic cell transplantation (HCT). Here we examine whether the combination of homeobox B4 (HOXB4) and Delta-1 ligand (DL) synergize when used together. Monkey and human UCB CD34(+) cells were transduced with a HOXB4-expressing gammaretroviral vector an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-286062
更新日期:2010-12-23 00:00:00
abstract::The von Willebrand factor propeptide, vW AgII, has been shown to be required for the formation of vWF multimers and sorting of vWF to storage granules; whether these 2 processes are independent events has been unclear. Chimeric constructs of human and canine vWF were developed to further define these processes and to ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::In this issue of Blood, Rowley et al report that noncoding RNAs precisely regulate the messenger RNA (mRNA) profile in platelets. Interfering in this process using genetically engineered mice affects hemostatic and thrombotic functions of platelets. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2016-01-694893
更新日期:2016-04-07 00:00:00
abstract::Twenty-one human immunodeficiency virus (HIV)-positive patients, including 11 acquired immunodeficiency syndrome (AIDS)-free patients with immune thrombocytopenic purpura (ITP), were studied to determine whether the megakaryocytic/platelet lineage was infected by HIV. Because purification of platelets did not reach a ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::Circulating lymphocytes leave the blood by binding to specialized high endothelial cells lining postcapillary venules in lymphoid organs or sites of chronic inflammations, migrating through the vessel wall into the surrounding tissue. The capacity of lymphocytes to recognize and bind to high endothelial venules (HEVs)...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00