Epitope specificity and V gene expression of cerebrospinal fluid T cells specific for intact versus cryptic epitopes of myelin basic protein.

Abstract:

:Recent evidence supports the possible involvement of myelin basic protein (BP) as one of the target autoantigens in multiple sclerosis (MS), including elevated frequencies of MS blood and cerebrospinal fluid (CSF) T cells, and the presence in MS plaque tissue of V beta gene sequences and CDR3 motifs characteristic of BP-reactive T cells. Because of its proximity to the target organ, the CSF has long been thought to harbor T cells involved in the pathogenic process. In order to evaluate their frequency and response characteristics, BP-reactive T cells were isolated by limiting dilution from the CSF of patients with MS and other neurological diseases (OND) for quantitation and determination of epitope specificity and V alpha and V beta gene expression. In addition to isolates responsive to intact BP epitopes that were present at a significantly higher frequency in MS versus OND CSF, we here describe a second clonotype responsive to 'cryptic' BP epitopes that is present at approximately equal frequencies in MS and OND patients. In spite of their difference in recognition of intact versus 'cryptic' BP determinants, both clonotypes predominantly recognized epitopes in the N terminal half of human BP, using a similar V gene repertoire that included biased use of V alpha 2 and to a lesser degree V beta 7 and V beta 18. These V gene biases were not related to the epitope specificity of the T cells, indicating that V gene selection is not epitope-driven. These data suggest that there is differential recognition of intact versus 'cryptic' BP determinants in MS versus OND patients that may be related to the processing and presentation of BP to the immune system.

journal_name

J Neuroimmunol

authors

Satyanarayana K,Chou YK,Bourdette D,Whitham R,Hashim GA,Offner H,Vandenbark AA

doi

10.1016/0165-5728(93)90268-4

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

57-67

issue

1

eissn

0165-5728

issn

1872-8421

pii

0165-5728(93)90268-4

journal_volume

44

pub_type

杂志文章
  • 6-Hydroxydopamine induces thymocyte apoptosis in mice.

    abstract::6-Hydroxydopamine (6-OHDA) induces degeneration of noradrenergic nerves and has been shown to alter the immune responses. In this study, intraperitoneal administration of 6-OHDA induces mouse thymus atrophy. The lowest levels of thymus weight and cell number were reached at days 3 and 5 in mice receiving 6-OHDA treatm...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(95)00166-2

    authors: Tsao CW,Cheng JT,Shen CL,Lin YS

    更新日期:1996-04-01 00:00:00

  • Thymocytes and cultured thymic epithelial cells express transcripts encoding alpha-3, alpha-5 and beta-4 subunits of neuronal nicotinic acetylcholine receptors: preferential transcription of the alpha-3 and beta-4 genes by immature CD4 + 8 + thymocytes.

    abstract::Thymic tissues express transcripts encoding the alpha-3, alpha-5 and beta-4 subunits of nicotinic neuronal acetylcholine receptors (AcChRs) suggesting that neuronal AcChRs similar to those expressed in ganglia are expressed in the thymus. Transcription occurs in both isolated thymocytes and thymic epithelial cells. RT...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/s0165-5728(97)00120-3

    authors: Mihovilovic M,Denning S,Mai Y,Whichard LP,Patel DD,Roses AD

    更新日期:1997-11-01 00:00:00

  • Increased serum acetylcholine receptor α1 subunit protein in anti-acetylcholine receptor antibody-positive myasthenia gravis.

    abstract::Complement-dependent disruption of motor endplate is detected in anti-acetylcholine receptor (AChR) antibody-positive myasthenia gravis (MG). We measured serum AChR α1 subunit protein levels, which may be associated with neuromuscular damage, in 55 patients with MG (47 were seropositive and 8 were negative) and in 20 ...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2019.577125

    authors: Uzawa A,Ozawa Y,Yasuda M,Oda F,Kojima Y,Kawaguchi N,Kanai T,Himuro K,Kuwabara S

    更新日期:2020-02-15 00:00:00

  • Occurrence and clinical relevance of an interleukin-4 gene polymorphism in patients with multiple sclerosis.

    abstract::An epistatic gene interaction has been advocated to explain disease susceptibility in multiple sclerosis (MS). Cytokine genes are possible candidates due to the central role played by cytokines in the regulation of the immune-mediated pathogenetic process leading to central nervous system demyelination in these patien...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/s0165-5728(97)00058-1

    authors: Vandenbroeck K,Martino G,Marrosu M,Consiglio A,Zaffaroni M,Vaccargiu S,Franciotta D,Ruggeri M,Comi G,Grimaldi LM

    更新日期:1997-06-01 00:00:00

  • T cell recognition of myelin proteolipid protein and myelin proteolipid protein peptides in the peripheral blood of multiple sclerosis and control subjects.

    abstract::Myelin proteolipid protein (PLP) is a prime candidate autoantigen for multiple sclerosis. In order to define potential immunodominant epitopes, T cell lines (TCL) from the peripheral blood of HLA-DR 15(2) MS patients were established which responded to the intact molecule of PLP. These TCL were then tested in individu...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/s0165-5728(97)00260-9

    authors: Trotter JL,Pelfrey CM,Trotter AL,Selvidge JA,Gushleff KC,Mohanakumar T,McFarland HF

    更新日期:1998-04-15 00:00:00

  • The conditioned enhancement of neutrophil activity is catecholamine dependent.

    abstract::Neutrophil activity was elevated in the conditioned mice for the first time through an established conditioned training process. Catecholamines were proved to be important in the regulation of this conditioned innate immunity. In the study, the camphor odor (as the conditioned stimulus, CS) and poly I: C (as the uncon...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2004.09.008

    authors: Chao HJ,Hsu YC,Yuan HP,Jiang HS,Hsueh CM

    更新日期:2005-01-01 00:00:00

  • Circulating CD56dim NK cells expressing perforin are increased in progressive multiple sclerosis.

    abstract::In this study we evaluated the percentages of CD3(-)CD56(bright), CD3(-)CD56(dim), CD3(-)CD56(bright)perforin(+) and CD3(-)CD56(dim)perforin(+) Natural Killer (NK) cells in peripheral blood from untreated secondary progressive (SP) and primary progressive (PP) multiple sclerosis (MS) patients and age and sex matched h...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2013.10.004

    authors: Plantone D,Marti A,Frisullo G,Iorio R,Damato V,Nociti V,Patanella AK,Bianco A,Mirabella M,Batocchi AP

    更新日期:2013-12-15 00:00:00

  • Visualisation of the kinetics of macrophage infiltration during experimental autoimmune encephalomyelitis by magnetic resonance imaging.

    abstract::Macrophages are considered to be the predominant effector cells in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Ultra small particles of iron oxide (USPIO) can be used to detect macrophage infiltrates in the CNS with magnetic resonance imaging (MRI). Here, we investigat...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2007.11.008

    authors: Baeten K,Hendriks JJ,Hellings N,Theunissen E,Vanderlocht J,Ryck LD,Gelan J,Stinissen P,Adriaensens P

    更新日期:2008-03-01 00:00:00

  • Encephalitogenic and immunogenic potential of myelin-associated glycoprotein (MAG), oligodendrocyte-specific glycoprotein (OSP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in ABH and SJL mice.

    abstract::Synthetic peptides of myelin-associated glycoprotein (MAG), oligodendrocyte-specific glycoprotein (OSP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) were screened for their ability to induce experimental allergic encephalomyelitis (EAE) in ABH (H-2A(g7)) and SJL (H-2(s)) mice. The use of overlapping 16mer...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/s0165-5728(01)00460-x

    authors: Morris-Downes MM,McCormack K,Baker D,Sivaprasad D,Natkunarajah J,Amor S

    更新日期:2002-01-01 00:00:00

  • Cell adhesion molecule expression in the regenerating rat facial nucleus.

    abstract::Transection of the rat facial nerve leads to an intrinsic microglial reaction in the facial nucleus. In the present study, we have examined immunocytochemically the expression of cell adhesion molecules during this process. Resting microglia constitutively expressed the LFA-1 alpha and beta chain (CD11a and CD18) in t...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(93)90181-w

    authors: Moneta ME,Gehrmann J,Töpper R,Banati RB,Kreutzberg GW

    更新日期:1993-06-01 00:00:00

  • Norepinephrine inhibits gamma-interferon-induced MHC class II (Ia) antigen expression on cultured brain astrocytes.

    abstract::Recent evidence that astrocytes can be induced to express the class II major histocompatability (MHC) antigens suggests that these cells may be involved in the development of intracerebral immune responses. The principal inducer of MHC class II antigen (Ia) expression is a soluble lymphokine, gamma-interferon (gamma-I...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(88)90017-3

    authors: Frohman EM,Vayuvegula B,van den Noort S,Gupta S

    更新日期:1988-01-01 00:00:00

  • Age-associated alterations in the time-dependent profile of pro- and anti-inflammatory proteins within the hippocampus in response to acute exposure to interleukin-1β.

    abstract::The pro-inflammatory cytokine IL-1β is known to play a role in several models of aging, neuroinflammation, and neurodegenerative diseases. Here, we document a detailed time- and age-dependent pattern of pro- and anti-inflammatory biomarkers following bilateral intrahippocampal injection of interleukin-1β. During the f...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2013.12.010

    authors: Hopp SC,Royer S,Brothers HM,Kaercher RM,D'Angelo H,Bardou I,Wenk GL

    更新日期:2014-02-15 00:00:00

  • Interferon-γ potentiates GABAA receptor-mediated inhibitory currents in rat hippocampal CA1 pyramidal neurons.

    abstract::The neural transmission and plasticity can be differentially modulated by various elements of the immune system. Interferon-γ (IFN-γ) is a "pro-inflammatory" cytokine mainly produced by T lymphocytes, activates its corresponding receptor and plays important roles under both homeostatic and inflammatory conditions. How...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2019.577050

    authors: Flood L,Korol SV,Ekselius L,Birnir B,Jin Z

    更新日期:2019-12-15 00:00:00

  • False positive immunoassay for acetyl choline receptor antibody (AChR Ab) in patients exposed to polyvalent antisnake venom.

    abstract::Acute flaccid paralysis is a neuromuscular emergency characterized by rapidly worsening weakness that evolves quickly to cause diaphragmatic failure. The challenge for the treating physician is to stabilize the patient, generate the differential diagnosis and determine the management; all in quick time. Neurotoxic sna...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2017.08.004

    authors: Sundar K,Venkatasubramanian S,Shanmugam S,Arthur P,Subbaraya R,Hazeena P

    更新日期:2017-10-15 00:00:00

  • The T cell-selective IL-2 mutant AIC284 mediates protection in a rat model of Multiple Sclerosis.

    abstract::Targeting regulatory T cells (Treg cells) with interleukin-2 (IL-2) constitutes a novel therapeutic approach for autoimmunity. As anti-cancer therapy with IL-2 has revealed substantial toxicities a mutated human IL-2 molecule, termed AIC284 (formerly BAY 50-4798), has been developed to reduce these side effects. To as...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2015.03.020

    authors: Weishaupt A,Paulsen D,Werner S,Wolf N,Köllner G,Rübsamen-Schaeff H,Hünig T,Kerkau T,Beyersdorf N

    更新日期:2015-05-15 00:00:00

  • Autoantibody repertoires to brain tissue in autism nuclear families.

    abstract::The hypothesis of an immune dysfunction in autism spectrum disorders has previously been put forward without, however, compelling evidence of a direct relation to its etiology or pathogenesis. To further understand if autoimmunity could play a significant role in autism, we analyzed autoantibody repertoires to brain t...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2004.03.015

    authors: Silva SC,Correia C,Fesel C,Barreto M,Coutinho AM,Marques C,Miguel TS,Ataide A,Bento C,Borges L,Oliveira G,Vicente AM

    更新日期:2004-07-01 00:00:00

  • Interferon beta in multiple sclerosis: relationship between sustained serum IgG levels and clinical outcome.

    abstract::We investigated the effects of interferon beta-1a (IFN beta-1a) on specific response towards two immunodominant MBP peptides and on global production of IgG. We evaluated 54 sera from multiple sclerosis (MS) patients at baseline and 1 year after treatment. We did not observe any modification of immune response to the ...

    journal_title:Journal of neuroimmunology

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0165-5728(02)00204-7

    authors: Dubucquoi S,de Seze J,Lefranc D,Almeras L,Dutoit V,Prin L,Vermersch P

    更新日期:2002-08-01 00:00:00

  • Influenza virus- and cytokine-immunoreactive cells in the murine olfactory and central autonomic nervous systems before and after illness onset.

    abstract::Influenza virus invades the olfactory bulb (OB) and enhances cytokine mRNAs therein at the time of illness onset. Here we show that viral antigen immunoreactivity co-localized with glial markers in the OB but could not be detected in other brain areas. Interleukin 1beta- and tumor necrosis factor alpha-immunoreactivit...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2009.03.016

    authors: Leyva-Grado VH,Churchill L,Wu M,Williams TJ,Taishi P,Majde JA,Krueger JM

    更新日期:2009-06-25 00:00:00

  • Strain differences in sleep and other pathophysiological sequelae of influenza virus infection in naive and immunized mice.

    abstract::To characterize behavioral and physiological alterations induced by viral respiratory infection, C57BL/6 and BALB/c strains of mice were monitored for 2 days before and 4 days after intranasal inoculation with influenza virus. Both strains developed hypothermia, decrease locomotor activity, and decreased delta-wave am...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(94)00193-r

    authors: Toth LA,Rehg JE,Webster RG

    更新日期:1995-04-01 00:00:00

  • Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

    abstract::Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-t...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2011.02.001

    authors: Biegler BW,Yan SX,Ortega SB,Tennakoon DK,Racke MK,Karandikar NJ

    更新日期:2011-05-01 00:00:00

  • Granulocyte-macrophage colony-stimulating factor as a mediator of autoimmunity in multiple sclerosis.

    abstract::Autoreactive, myelin-specific, CD4+ T cells have a central role in multiple sclerosis (MS) pathogenesis; however the exact phenotype characteristics of these cells remain elusive. Recently, granulocyte-macrophage colony-stimulating factor (GM-CSF) expression has emerged as the main pathological signature of the enceph...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneuroim.2018.07.002

    authors: Kostic M,Zivkovic N,Cvetanovic A,Stojanovic I

    更新日期:2018-10-15 00:00:00

  • The autoimmune T and B cell repertoires in monozygotic twins discordant for myasthenia gravis.

    abstract::Two pairs of monozygotic twins, discordant for myasthenia gravis (MG) for more than 30 years, were studied regarding T cell and antibody reactivity against disease related autoantigens, the acetylcholine receptor, one idiotypic and one anti-idiotypic human monoclonal antibody. The healthy and myasthenic twins had very...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2003.09.019

    authors: Kakoulidou M,Ahlberg R,Yi Q,Giscombe R,Pirskanen R,Lefvert AK

    更新日期:2004-03-01 00:00:00

  • Alternative splicing of interleukin-7 (IL-7) and interleukin-7 receptor alpha (IL-7Ralpha) in peripheral blood from patients with multiple sclerosis (MS).

    abstract::IL-7 and IL-7Ralpha (IL-7R) form a non-redundant ligand receptor system which plays a crucial role in human T cell immunity. Both IL-7 and IL-7R are multi-exonal genes and exhibit alternative splicing. We measured the relative distribution of IL-7 and IL-7R spliced mRNA from patients with MS and healthy individuals an...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2010.02.014

    authors: Rane L,Vudattu N,Bourcier K,Graniar E,Hillert J,Seyfert V,Maeurer MJ

    更新日期:2010-05-01 00:00:00

  • Peroxisome proliferator-activated receptor-gamma agonists inhibit the activation of microglia and astrocytes: implications for multiple sclerosis.

    abstract::Peroxisome proliferator-activated receptor (PPAR)-gamma agonists, including thiazolidinediones (TZDs) and 15-deoxy-Delta(12,14) prostaglandin J(2) (15d-PGJ(2)), have been shown to be effective in the treatment of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). This study ai...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2004.12.015

    authors: Storer PD,Xu J,Chavis J,Drew PD

    更新日期:2005-04-01 00:00:00

  • TRPV1 polymorphisms and risk of interferon β-induced flu-like syndrome in patients with relapsing-remitting multiple sclerosis.

    abstract::Interferon-β (IFN-β) is often discontinued in RRMS patients due to its most common side effect, the flu-like syndrome (FLS). The mechanisms underlying IFN β-induced FLS symptoms are still unclear. The endocannabinoid system (ECS) is a key regulator of pain and inflammation. Thus we tested the hypothesis that the ECS c...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2017.02.007

    authors: Buttari F,Zagaglia S,Marciano L,Albanese M,Landi D,Nicoletti CG,Mercuri NB,Silvestrini M,Provinciali L,Marfia GA,Mori F,Centonze D

    更新日期:2017-04-15 00:00:00

  • TDP-43 proteolysis is associated with astrocyte reactivity after traumatic brain injury in rodents.

    abstract::The aggregation and deposition of transactivation response DNA-binding protein 43 (TDP-43) in neurons and astrocytes is characteristic in a number of neurodegenerative diseases including Alzheimer's disease, frontotemporal lobar degeneration, and amyotrophic lateral sclerosis. Nevertheless, the exact role of TDP-43 in...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2017.10.011

    authors: Huang CY,Lee YC,Li PC,Liliang PC,Lu K,Wang KW,Chang LC,Shiu LY,Chen MF,Sun YT,Wang HK

    更新日期:2017-12-15 00:00:00

  • Lymphocytic choriomeningitis virus induces a chronic wasting disease in mice lacking class I major histocompatibility complex glycoproteins.

    abstract::Lymphocytic choriomeningitis virus (LCMV) induces a chronic, wasting syndrome when injected intracerebrally into H-2b mice homozygous for a beta 2-microglobulin (beta 2-m (-/-)) gene disruption. These mice have very few CD8+ T cells and express little class I MHC glycoprotein, though minimal levels of the H-2Db molecu...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(93)90228-q

    authors: Doherty PC,Hou S,Southern PJ

    更新日期:1993-07-01 00:00:00

  • Interferon-beta specifically inhibits interferon-gamma-induced class II major histocompatibility complex gene transcription in a human astrocytoma cell line.

    abstract::We established cultures of human astrocytes and astrocytoma cells from surgical specimens, to study regulation of class II major histocompatibility (MHC) complex antigen expression by interferons. Using these cultures we previously showed that expression of the class II MHC determinant HLA-DR could be induced by inter...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(91)90054-b

    authors: Ransohoff RM,Devajyothi C,Estes ML,Babcock G,Rudick RA,Frohman EM,Barna BP

    更新日期:1991-08-01 00:00:00

  • Pattern recognition receptor mRNA expression and cytokine and granzyme levels in HIV infected individuals with neurotuberculosis.

    abstract::Neurotuberculosis is one of the commonest HIV-associated opportunistic infections (OI) of the CNS. Cross-talk between HIV, Mycobacterium tuberculosis and host immune responses may alter expression of pattern recognition receptors (PRRs), thereby affecting cytokine profiles and functional responses. We examined PRR mRN...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/j.jneuroim.2018.01.015

    authors: Rao D,Vasanthapuram R,Satishchandra P,Desai A

    更新日期:2018-05-15 00:00:00

  • Beta-endorphin protects mice from neurological disease induced by the murine coronavirus MHV-JHM.

    abstract::The neurotropic murine coronavirus, MHV-JHM (JHMV) causes encephalitis and paralytic-demyelinating disease in susceptible strains of mice and rats, serving as a model for human demyelinating diseases such as multiple sclerosis. In this communication, we report that a single intracerebral administration of the naturall...

    journal_title:Journal of neuroimmunology

    pub_type: 杂志文章

    doi:10.1016/0165-5728(93)90061-3

    authors: Gilmore W,Moradzadeh DS

    更新日期:1993-10-01 00:00:00