Abstract:
:The responses of slice-cultured Purkinje cells to trans-DL-1-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD) were examined by intracellular recording techniques and fura-2 microfluorometry. Bath-application of t-ACPD (100 microM, 30 s), a selective agonist of metabotropic glutamate receptors (mGluRs), to Purkinje cells voltage-clamped near their resting potential -65 to -60 mV) consistently induced a transient inward current, followed by a slower outward current (Iout). This outward current was characterized by a linear current-voltage relationship in the range from -130 to -60 mV and accompanied by a significant decrease in membrane conductance. The extrapolated reversal potential of Iout was positive to 0 mV. When t-ACPD was applied for 60 s or more it became apparent that Iout emerged in parallel to the wash-out of t-ACPD. Microfluorometric fura-2 measurements in combination with electrophysiological recordings were used to assess the relation between Iout and intracellular free calcium concentration ([Ca2+]i). In contrast to the inward current that was associated with a transient elevation in [Ca2+]i. Iout was not correlated with an elevated [Ca2+]i. When t-ACPD was applied in the presence of caffeine (5 mM), Iout was reversibly enhanced in amplitude. Caffeine affected neither the t-ACPD-induced calcium signal nor the resting [Ca2+]i. While longer applications of caffeine alone induced outward currents with a current-voltage relationship similar to that of Iout, short applications (30 s) of caffeine had no detectable effect per se but still were effective in enhancing Iout when applied in conjunction with t-ACPD. 3-Isobutyl-1-methylxanthine (IBMX, 0.5 mM), a more selective and potent phosphodiesterase inhibitor than caffeine, exhibited caffeine-like effects at a 10-fold lower concentration. We propose that Iout is generated by a transient inhibition of an inward current that is tonically active at rest and largely voltage-independent in the range tested. Our observations provide evidence for an involvement of cyclic nucleotide second messenger systems in the regulation of this current.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Vranesic I,Staub C,Knöpfel Tdoi
10.1016/0168-0102(93)90125-asubject
Has Abstractpub_date
1993-03-01 00:00:00pages
209-15issue
3eissn
0168-0102issn
1872-8111pii
0168-0102(93)90125-Ajournal_volume
16pub_type
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