Abstract：
:Metallothioneins (MT) are common proteins in animal tissues. These proteins take part in the homeostasis of the ions of the metals which are necessary for the proper metabolism of the organism (zinc, copper), biosynthesis regulation and zincprotein activity (for example the activity of the zinc-dependant transcription factors) and they also take part in the detoxication of the tissue from toxic metals. Apart from these, they also protect the tissue from reactive oxygen species, radiation, electrophilic pharmacological agents used in the cancer therapy and the mutagens. The aim of this work was to obtain cellular sub-fractions of brain tumors and to separate these proteins by SDS-polyacrylamide gel electrophoresis and Western Blotting technique and to determine the level of metallothioneins and to determine the level of metallthioneins in the cellular sub-fraction. The experimental materials were the brain neoplastic tissues resected during neurosurgical procedures. The brain tumors were divided into two groups; astrocytoma G-2 and malignant gliomas (astrocytoma GM-4, glioblastoma multiforme). The cellular fractions of tumour tissues were obtained according to Clark and Nicklas and Chauveau et al. methods. The level of the metallothioneins was determined by the cadmium-hemoglobin affinity assay using the cadmium isotope (109Cd). By performing protein dissection on polyacrylamide gel (PAGE) and applying immunoidentifying technique we proved the presence of metallothioneins in all the cell sub-fractions. In GM-4 patients the total MT level increased by 12.06% whereas in the cytosol sub-fraction it increased by 17.02% in comparison with astrocytoma G-2 patients. In the mitochondrial sub-fraction the level increased by 49.09% while in the nuclear and microsomal sub-fractions the increase was by 22.38 and 8.24%, respectively.
journal_name
Folia Neuropatholjournal_title
Folia neuropathologicaauthors
Floriańczyk B,Osuchowski J,Kaczmarczyk R,Starosławska E,Trojanowski Tsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
91-6issue
2eissn
1641-4640issn
1509-572Xpii
3430journal_volume
43pub_type
杂志文章abstract：:The post-mortem neuropathological investigations were carried out on 20 female New Zealand rabbits. Two main types of changes were found: inflammatory, including meningeal and perivascular infiltrates, and thrombotic within the nervous tissue. The findings revealed that active process within the CNS persists at least ...
journal_title：Folia neuropathologica
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journal_title：Folia neuropathologica
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journal_title：Folia neuropathologica
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更新日期：2000-01-01 00:00:00
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journal_title：Folia neuropathologica
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更新日期：2004-01-01 00:00:00
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journal_title：Folia neuropathologica
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更新日期：2002-01-01 00:00:00
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journal_title：Folia neuropathologica
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journal_title：Folia neuropathologica
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更新日期：2004-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Folia neuropathologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia neuropathologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2011-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2008-01-01 00:00:00
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journal_title：Folia neuropathologica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Folia neuropathologica
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更新日期：2001-01-01 00:00:00
abstract：:Paralytic tremor (pt), a hereditary neurological disorder of rabbits, is a recessive, X-linked point mutation in exon 2 of the plp gene, responsible for substitution of 38 His by Glu in the PLP molecule. Pt genotype is expressed in a range of phenotypes, distinguished by the severity of neurological symptoms. Variable...
journal_title：Folia neuropathologica
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更新日期：2006-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2007-01-01 00:00:00
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journal_title：Folia neuropathologica
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doi：
更新日期：2010-01-01 00:00:00
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journal_title：Folia neuropathologica
pub_type： 杂志文章,评审
doi：
更新日期：1994-01-01 00:00:00
abstract：:This paper presents ultrastructural changes in neuronal and glial cells with special reference to intranuclear inclusion bodies in subacute sclerosing panencephalitis (SSPE) with different duration (from several weeks to seven years). Brain autopsy at ultrastructural level revealed the nucleocapsids of paramyxovirus i...
journal_title：Folia neuropathologica
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更新日期：2001-01-01 00:00:00
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abstract：INTRODUCTION:Brain injury caused by ischaemic stroke is a major cause of disability and death throughout the world. The present study evaluates the neuroprotective effect of pseudopterosin A (PtA) against ischaemia-induced brain injury. MATERIAL AND METHODS:Ischaemia was induced by pMCAO model, and rats were separated...
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更新日期：2018-01-01 00:00:00
