Abstract：
:Human epidermis consists of a stratified epithelium mainly composed of keratinocytes and relies on a stem cell compartment to undergo constant regeneration. Genetic mutations affecting the capacity of basal keratinocytes to adhere firmly to the epidermal basement membrane lead to severe, and very often lethal, blistering disorders known as epidermolysis bullosa. Gene therapy represents a promising potential treatment for these devastating inherited disorders. Human epidermal stem cells can be cultivated ex vivo and stably transduced with integrating gene transfer vectors, allowing genetic and, more important, phenotypic correction of the adhesion properties of keratinocytes. Here we will review some of the issues that need to be addressed to make gene therapy a realistic treatment for these disorders, such as (1) which cells should be targeted, (2) which approach (in vivo or ex vivo) should be chosen, and (3) which gene transfer vector (retrovirus, lentivirus, or integrating nonviral strategies) should be used for stable gene correction. In the last 10 years, many reports have shown that gene transfer approaches to target epidermal stem cells are feasible and able to restore the adhesion properties of primary keratinocytes from patients with epidermolysis bullosa. In addition, tremendous progress has been achieved in culturing epidermal stem cells and generating sheets of stratified epithelium for permanent coverage of full-thickness burns. Gene modification of stem cells in combination with advanced tissue-engineering techniques could therefore represent a realistic option for patients with epidermolysis bullosa.
journal_name
Clin Dermatoljournal_title
Clinics in dermatologyauthors
Ferrari S,Pellegrini G,Mavilio F,De Luca Mdoi
10.1016/j.clindermatol.2004.07.017subject
Has Abstractpub_date
2005-07-01 00:00:00pages
430-6issue
4eissn
0738-081Xissn
1879-1131pii
S0738-081X(04)00145-2journal_volume
23pub_type
杂志文章,评审abstract：:Skin, together with its appendages, forms an organ with several defensive roles; among them it acts as a barrier, which is one of its very important protective functions. There has been increased interest in studying the effects of age on these functions. There are different intensities and timing involved in this pro...
journal_title：Clinics in dermatology
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journal_title：Clinics in dermatology
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abstract：:Recently, dermatology group practice ownership structures have changed, as private equity (PE) consolidates independent dermatology practices to create larger groups. Currently, little is known about how dermatology residents perceive practice ownership structures, including those that are owned by PE. One hundred thi...
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2003.12.017
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journal_title：Clinics in dermatology
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2006.07.013
更新日期：2006-11-01 00:00:00
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/j.clindermatol.2008.10.007
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2004.03.011
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2008.01.011
更新日期：2009-03-01 00:00:00
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journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/0738-081x(96)00048-x
更新日期：1996-09-01 00:00:00
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journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/0738-081x(87)90031-9
更新日期：1987-10-01 00:00:00
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journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/j.clindermatol.2009.02.005
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2009.03.009
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章
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journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/j.clindermatol.2011.11.013
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
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journal_title：Clinics in dermatology
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journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2003.12.030
更新日期：2004-01-01 00:00:00
abstract：:The metabolic syndrome (MetS) or insulin resistance syndrome is a constellation of obesity-related metabolic derangements predisposing to type 2 diabetes and cardiovascular disease. In 1998, WHO defined the first criteria of MetS. Three years later, the user-friendly National Cholesterol Education Program criteria of ...
journal_title：Clinics in dermatology
pub_type： 历史文章,杂志文章
doi：10.1016/j.clindermatol.2017.09.002
更新日期：2018-01-01 00:00:00
abstract：:The world population of adults aged 60 years or more is increasing globally, and this development can impact skin disease morbidity and mortality, as well as being reflected in the health care system organization. There is substantial evidence that the burden from a remarkable number of skin nonmalignant and malignant...
journal_title：Clinics in dermatology
pub_type： 杂志文章
doi：10.1016/j.clindermatol.2019.04.005
更新日期：2019-01-01 00:00:00
abstract：:Variations in pain perception have long been studied in the medical literature, especially for women in regards to their menstrual cycle. Anecdotally, women can be more sensitive to painful stimuli from cosmetic procedures during their menses; however, no studies have thoroughly examined variations in pain perception ...
journal_title：Clinics in dermatology
pub_type： 杂志文章,评审
doi：10.1016/j.clindermatol.2019.10.011
更新日期：2019-01-01 00:00:00
