Abstract:
:Intraoperative irradiation (IORT) is used to enhance local tumour control by using large, single doses while removing critical structures from the treatment field. Peripheral nerve remains a dose-limiting normal tissue that often cannot be removed from the field. To assess ultrastructural changes in canine sciatic nerve after IORT, computerized morphometric analysis of plastic sections and electron micrographs of nerve cross-sections was used. Surgically exposed sciatic nerves were irradiated with 6 MeV electrons to 12, 20 or 28 Gy. Twelve months after treatment dogs were killed humanely and the nerves from three dogs per dose group, including non-irradiated controls, were analyzed. Twelve months after 28-Gy IORT a significant decrease in nerve fiber density occurred. Nerve fiber loss was particularly prominent in the central portion of the nerve predominantly among large nerve fibers. Other nerve fiber parameters including fiber and axon area, diameter and perimeter, myelin thickness, form factor (measure of roundness), and G ratio (axon diameter/fiber diameter) did not show significant, dose-related changes. An increase in microtubule and neurofilament density in irradiated nerve axons was found. These changes are suggestive of radiation-induced hypoxia (damage to microvasculature) resulting in axon damage and subsequent nerve fiber loss as a possible mechanism of late radiation injury to peripheral nerve.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Vujaskovic Z,Gillette SM,Powers BE,Thurmond DN,Gillette EL,Colacchio TAdoi
10.1080/09553009514550941subject
Has Abstractpub_date
1995-07-01 00:00:00pages
71-6issue
1eissn
0955-3002issn
1362-3095journal_volume
68pub_type
杂志文章abstract::The repair of DNA double-strand breaks was measured by pH 7.2 filter elution in cells incubated at 25-45 degrees C either before or after X-irradiation. Exposure to 45 degrees C for 15 minutes immediately prior to X-irradiation significantly increased both the half-time for DNA double-strand break closure and the numb...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214550601
更新日期:1992-01-01 00:00:00
abstract::Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentratio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146228
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To examine whether the levels of micronuclei induction, as a marker for genomic instability in the progeny of X-irradiated cells, correlates with DNA repair function. MATERIALS AND METHODS:Two repair deficient cell lines (X-ray repair cross-complementing 1 [XRCC1] deficient cell line [EM9] and X-ray repair cro...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.752596
更新日期:2013-04-01 00:00:00
abstract:PURPOSE:Epidemiological studies have demonstrated a possible correlation between exposure to extremely low-frequency magnetic fields (ELF-MF) and cancer. However, this correlation has yet to be definitively confirmed by epidemiological studies. The principal objective of this study was to assess the effects of 60 Hz ma...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.652724
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141771
更新日期:1998-07-01 00:00:00
abstract:PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701317366
更新日期:2007-06-01 00:00:00
abstract::A new approach is reported that combines synchrotron radiolysis and mass spectrometry to probe the structure of proteins. Hydroxyl radicals produced upon the radiolysis of protein solutions using synchrotron light modify amino acid side-chains on millisecond timescales. This results in the formation of stable oxidatio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110094805
更新日期:2002-02-01 00:00:00
abstract::Despite the extensive utilization of radiotherapy in cancer treatments, clinicians often face challenges in achieving desired outcomes. This is because of the existence of secondary radiation effects like bystander phenomenon that influence radiation nontargeted cells and tissues. The concept of bystander effects of r...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1547440
更新日期:2019-03-01 00:00:00
abstract::Radioiodine ((131)I) induced a dose- and time-dependent increase in DNA single-strand breaks (DNA-ssb) in human (G0) mononuclear blood cells (MNC) in vitro. Incubation of MNC with 22MBq (131)I/ml at 4 degrees C caused a linear, time-dependent induction of DNA-ssb (increase in elution rate: 24.7 x 10(-3) h(-1) per 100 ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143103
更新日期:1997-11-01 00:00:00
abstract::One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mito...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551771
更新日期:1988-09-01 00:00:00
abstract::Purpose: This review discusses recent developments in our understanding of biological and physiological mechanisms underlying radiation cataractogenesis. The areas discussed include effects of low-dose exposures to the lens including potential relevance of non-targeted effects, the development of new personal-protecti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1767820
更新日期:2020-05-26 00:00:00
abstract::Murine bone marrow contains osteogenic precursor cells that undergo differentiation during in vitro cultivation. In vitro these cells are potential target cells for alpha-irradiation-induced bone tumour formation. Under defined tissue culture conditions these differentiating cells were directly exposed to alpha-partic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551111
更新日期:1994-08-01 00:00:00
abstract:PURPOSE:The identification of fibroblast-like cells of the marrow stroma by means of alkaline phosphatase (ALP) cytochemistry reveals delicate ALP-positive structures interspersed among haematopoietic cells and arranged in a loosely meshed network. These cells are often referred to as 'reticular' cells and the network ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000085740
更新日期:2003-04-01 00:00:00
abstract::This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097142834
更新日期:1997-12-01 00:00:00
abstract:PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139494
更新日期:1999-10-01 00:00:00
abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1562254
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:Radioresistance is highly correlated with radiotherapy failure in clinical cancer treatment. In the current study, we sought to examine the efficacy of Celecoxib and Afatinib co-treatment as radiosensitizers in the management of non-small cell lung cancer (NSCLC) A549 cells. MATERIALS AND METHODS:Generally, A5...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1846817
更新日期:2020-11-12 00:00:00
abstract:PURPOSE:Cells derived from individuals in which the ataxia telangiectasia (ATM) gene is mutated are hypersensitive to ionizing radiation. Whether differences in ATM protein levels exist among human malignant glioma cell lines and whether such differences are correlated with cellular radiosensitivity were determined. M...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141591
更新日期:1998-08-01 00:00:00
abstract:PURPOSE:To investigate the relationship between the susceptibility of B-chronic lymphoid leukaemia (B-CLL) cells to DNA damage-induced apoptosis, the kinetics of DNA strand-break rejoining, and chromosome damage after exposure to ionizing irradiation. MATERIALS AND METHODS:Lymphocytes from B-CLL patients were gamma-ir...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110120364
更新日期:2002-08-01 00:00:00
abstract:PURPOSE:The aim is to assess the change with age and disease of the oxygen concentration within the coronary artery walls. MATERIALS AND METHODS:In an accompanying paper, Part A, the age-dependent morphology and composition for the wall layers of normal and diseased coronary arteries were developed from published data...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802389645
更新日期:2008-10-01 00:00:00
abstract:PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110075220
更新日期:2001-12-01 00:00:00
abstract:PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1427903
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Ionizing radiation (IR) produced a dose-dependent increase in apoptosis in U937/pCEP4 cells which was attenuated by the stable over expression of Bcl-2 (U937/Bcl-2). A dose of 2 Gy IR was selected for further analyses to determine if subsequent exposure to 10nM bryostatin- would overcome the resistance to IR-in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050151592
更新日期:2000-10-01 00:00:00
abstract:PURPOSE:Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and phosphorylation in hum...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.634882
更新日期:2012-03-01 00:00:00
abstract:PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138745
更新日期:2000-03-01 00:00:00
abstract:PURPOSE:Ifosfamide as a chemotherapeutic drug is used for the treatment of different cancer types. The purpose of this study is the preparation of 99mTc-ifosfamide complex to be evaluated as a potential candidate for tumor imaging. MATERIALS AND METHODS:The radiolabeling of ifosfamide with technetium-99m was carried o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1524945
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:To evaluate the radioprotective activity of C3, a regioisomer of water-soluble carboxyfullerene and a potent free radical scavenger, on both normal and tumour cells. MATERIALS AND METHODS:The murine committed bone-marrow stem cells for both granulocytes and monocytes (GM-CFC) were used to represent normal cell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001750068966
更新日期:2001-02-01 00:00:00
abstract:PURPOSE:To illustrate how probability-generating functions (PGFs) can be employed to derive a simple probabilistic model for clonogenic survival after exposure to ionizing irradiation. METHODS:Both repairable and irreparable radiation damage to DNA were assumed to occur by independent (Poisson) processes, at intensiti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138600
更新日期:2000-04-01 00:00:00
abstract::The radiation response of 15 mammalian cell lines comprising 11 human tumour, two human fibroblast and two murine lymphoma cell lines, has been analysed using the linear-quadratic equation. As well as using conventional analysis of acute dose-survival curves to derive values for alpha and beta (termed alpha ac and bet...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551241
更新日期:1992-04-01 00:00:00
abstract:PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141672
更新日期:1998-07-01 00:00:00