Covalently closed circular DNA is the predominant form of duck hepatitis B virus DNA that persists following transient infection.

abstract：:Brome mosaic virus (BMV) RNA replication is directed by two virus-encoded proteins, 1a and 2a. The amino-terminal half of 1a is a distant homolog of alphavirus nonstructural protein nsP1, which has been implicated in capping viral RNAs. In this study, we examined the enzymatic activities of BMV 1a expressed in yeast, ...

journal_title：Journal of virology

authors： Ahola T,Ahlquist P

更新日期：1999-12-01 00:00:00

abstract：:Cholesterol plays an essential role in the life cycle of several enveloped viruses. Many of these viruses manipulate host cholesterol metabolism to facilitate their replication. HIV-1 infection of CD4(+) T cells activates the sterol regulatory element-binding protein 2 (SREBP2) transcriptional program, which includes ...

journal_title：Journal of virology

authors： Taylor HE,Linde ME,Khatua AK,Popik W,Hildreth JE

更新日期：2011-08-01 00:00:00

abstract：:Transient lymphopenia is a hallmark of measles virus (MV)-induced immunosuppression. To address to what extent replenishment of the peripheral lymphocyte compartment from bone marrow (BM) progenitor/stem cells might be affected, we analyzed the interaction of wild-type MV with hematopoietic stem and progenitor cells (...

journal_title：Journal of virology

authors： Boussaad I,Varagnolo L,Hornich V,Rieger L,Krockenberger M,Stuehmer T,Kranzfelder D,Mueller AM,Schneider-Schaulies S

更新日期：2011-08-01 00:00:00

abstract：:The structural gene for diphtherial toxin is present in corynebacteriophage beta. Previous studies located several point mutations within the tox locus and determined the orientation of transcription of the toxin structural gene. The production of maximal quantities of toxin by Corynebacterium diphtheriae C7(beta) occ...

journal_title：Journal of virology

authors： Welkos SL,Holmes RK

更新日期：1981-03-01 00:00:00

abstract：:In bacterial strains containing the deoxyribonuclease endonuclease I (endonuclease I(+) strains), 70 to 80% of the injected superinfecting T-even phage deoxyribonucleic acid (DNA) is rapidly degraded to oligonucleotides having an average chain length of 8, the same value as that obtained by endonuclease I digestion of...

journal_title：Journal of virology

authors： Anderson CW,Eigner J

更新日期：1971-12-01 00:00:00

abstract：:Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the role of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites in hypervariable regions (V1 to V3) of the env gene ...

journal_title：Journal of virology

authors： Li Y,Cleveland B,Klots I,Travis B,Richardson BA,Anderson D,Montefiori D,Polacino P,Hu SL

更新日期：2008-01-01 00:00:00

abstract：:We previously reported that a recombinant pantothenate auxotroph of Mycobacterium bovis BCG expressing human immunodeficiency virus type 1 (HIV-1) subtype C Gag (rBCGpan-Gag) efficiently primes the mouse immune system for a boost with a recombinant modified vaccinia virus Ankara (rMVA) vaccine. In this study, we furth...

journal_title：Journal of virology

authors： Chege GK,Burgers WA,Stutz H,Meyers AE,Chapman R,Kiravu A,Bunjun R,Shephard EG,Jacobs WR Jr,Rybicki EP,Williamson AL

更新日期：2013-05-01 00:00:00

abstract：:Influenza viruses unable to express NS1 protein (delNS1) replicate poorly and induce large amounts of interferon (IFN). They are therefore considered candidate viruses for live-attenuated influenza vaccines. Their attenuated replication is generally assumed to result from the inability to counter the antiviral host re...

journal_title：Journal of virology

authors： van Wielink R,Harmsen MM,Martens DE,Peeters BP,Wijffels RH,Moormann RJ

更新日期：2012-11-01 00:00:00

abstract：:Among prion infections, two scenarios of prion spread are generally observed: (i) early lymphoid tissue replication or (ii) direct neuroinvasion without substantial antecedent lymphoid amplification. In nature, cervids are infected with chronic wasting disease (CWD) prions by oral and nasal mucosal exposure, and studi...

journal_title：Journal of virology

authors： Hoover CE,Davenport KA,Henderson DM,Denkers ND,Mathiason CK,Soto C,Zabel MD,Hoover EA

更新日期：2017-04-28 00:00:00

abstract：:Vaccination for human immunodeficiency virus type 1 (HIV-1) remains an elusive goal. Whether an unsuccessful vaccine might not only fail to provoke detectable immune responses but also could actually interfere with subsequent natural immunity upon HIV-1 infection is unknown. We performed detailed assessment of an HIV-...

journal_title：Journal of virology

authors： Balamurugan A,Lewis MJ,Kitchen CM,Robertson MN,Shiver JW,Daar ES,Pitt J,Ali A,Ng HL,Currier JS,Yang OO

更新日期：2008-03-01 00:00:00

abstract：:We established previously that the temperature-dependent host range mutant, td CE 3, of vesicular stomatitis virus (VSV) New Jersey possesses temperature-sensitive RNA transcriptase activity. In this paper, we describe dissociation and reconstitution experiments designed to determine which VSV polypeptide is affected ...

journal_title：Journal of virology

authors： Szilágyi JF,Pringle CR,Macpherson TM

更新日期：1977-05-01 00:00:00

abstract：:The ICP18.5 gene (UL28) of herpes simplex virus type 1 is a member of a well-conserved gene family among herpesviruses and is thought to play a role in localization of viral glycoproteins. We have cloned, sequenced, and expressed the entire pseudorabies virus (PRV) ICP18.5 open reading frame in Escherichia coli as a C...

journal_title：Journal of virology

authors： Pederson NE,Enquist LW

更新日期：1991-07-01 00:00:00

abstract：:An adeno-associated virus (AAV) genome with a Lys-to-His (K340H) mutation in the consensus nucleotide triphosphate binding site of the rep gene has a dominant-negative DNA replication phenotype in vivo. We expressed both wild-type (Rep78) and mutant (Rep78NTP) proteins in two helper-free expression systems consisting ...

journal_title：Journal of virology

authors： Owens RA,Carter BJ

更新日期：1992-02-01 00:00:00

abstract：:The mature virion of the alphaherpesvirus pseudorabies virus (PrV) contains a minimum of 31 structural proteins which are recruited into the virus particle by a network of protein-protein interactions which is only incompletely understood. We show here that deletion of the tegument protein pUL21 resulted in a drastic ...

journal_title：Journal of virology

authors： Michael K,Klupp BG,Karger A,Mettenleiter TC

更新日期：2007-01-01 00:00:00

abstract：:The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture sy...

journal_title：Journal of virology

authors： Van Etten JL,Burbank DE,Cuppels DA,Lane LC,Vidaver AK

更新日期：1980-02-01 00:00:00

abstract：:Production of a vesicular stomatitis virus spike protein G (VSVG)-pseudotyped lentiviral expression vector in HEK293 cells decreased on overexpression of low-density lipoprotein receptor (LDLR) but not that of ICAM1 or TfR1. Reverse transcription-quantitative PCR (RT-qPCR) revealed a reduction in vector RNA as a funct...

journal_title：Journal of virology

authors： Otahal A,Fuchs R,Al-Allaf FA,Blaas D

更新日期：2015-11-01 00:00:00

abstract：:The characteristics of fusion of respiratory syncytial virus (RSV) with HEp-2 cells were studied by the R18 fluorescence dequenching assay of membrane fusion. A gradual increase in fluorescence intensity indicative of virion-cell fusion was observed when R18-labeled RSV was incubated with HEp-2 cells. Approximately 35...

journal_title：Journal of virology

authors： Srinivasakumar N,Ogra PL,Flanagan TD

更新日期：1991-08-01 00:00:00

abstract：:Enterovirus 71 (EV71) (genus Enterovirus, family Picornaviridae), a common cause of hand, foot, and mouth disease (HFMD), may also cause severe neurological diseases, such as encephalitis and poliomyelitis-like paralysis. To examine the genetic diversity and rate of evolution of EV71, we have determined and analyzed c...

journal_title：Journal of virology

authors： Brown BA,Oberste MS,Alexander JP Jr,Kennett ML,Pallansch MA

更新日期：1999-12-01 00:00:00

abstract：:Using synthetic peptides, we characterized the B-lymphocyte (antibody) and T-lymphocyte (proliferation) responses to an immunodominant epitope of human immunodeficiency virus type 1 (HIV-1) located near the amino-terminal end of the transmembrane glycoprotein (env amino acids 598 to 609). Both immunoglobulin M (IgM) a...

journal_title：Journal of virology

authors： Schrier RD,Gnann JW Jr,Langlois AJ,Shriver K,Nelson JA,Oldstone MB

更新日期：1988-08-01 00:00:00

abstract：:Viral hemorrhagic septicemia virus (VHSV) infections cause high losses in cultured rainbow trout in Europe. Attempts to produce a recombinant vaccine based on the transmembrane glycoprotein (G protein) have indicated that proper folding is important for the antigenicity and immunogenicity of the protein. The present s...

journal_title：Journal of virology

authors： Einer-Jensen K,Krogh TN,Roepstorff P,Lorenzen N

更新日期：1998-12-01 00:00:00

abstract：:Kilham rat virus (KRV) was grown in a rat nephroma cell line and was purified by two isopycnic centrifugations in cesium chloride. The virus contains single-stranded deoxyribonucleic acid (DNA) with a molecular weight of approximately 1.6 x 10(6). The DNA was extracted from the virion by both phenol extraction and by ...

journal_title：Journal of virology

authors： Salzman LA,White WL,Kakefuda T

更新日期：1971-06-01 00:00:00

abstract：:The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...

journal_title：Journal of virology

authors： Youngner JS,Feingold DS

更新日期：1967-12-01 00:00:00

abstract：:Innate immunity provides an immediate defense against infection after host cells sense danger signals from microbes. Endoplasmic reticulum (ER) stress arises from accumulation of misfolded/unfolded proteins when protein load overwhelms the ER folding capacity, which activates the unfolded protein response (UPR) to res...

journal_title：Journal of virology

authors： Frabutt DA,Wang B,Riaz S,Schwartz RC,Zheng YH

更新日期：2017-12-14 00:00:00

abstract：:An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...

journal_title：Journal of virology

authors： De Leys R,Vanderborght B,Vanden Haesevelde M,Heyndrickx L,van Geel A,Wauters C,Bernaerts R,Saman E,Nijs P,Willems B

更新日期：1990-03-01 00:00:00

abstract：:There are two types of infectious vaccinia virus particles: intracellular naked virions and extracellular enveloped virions (EEV). To determine the biological role of the enveloped form of vaccinia virus, we produced and characterized a mutant that is defective in EEV formation. The strategy involved replacement by ho...

journal_title：Journal of virology

authors： Blasco R,Moss B

更新日期：1991-11-01 00:00:00

abstract：:The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data pr...

journal_title：Journal of virology

authors： Gilbert JM,Benjamin TL

更新日期：2000-09-01 00:00:00

abstract：:N-terminal modifications of the chemokine RANTES bind to C-C chemokine receptor 5 (CCR5) and block human immunodeficiency virus type 1 (HIV-1) infection with greater efficacy than native RANTES. Modified RANTES compounds induce rapid CCR5 internalization and much slower receptor reexpression than native RANTES, sugges...

journal_title：Journal of virology

authors： Sabbe R,Picchio GR,Pastore C,Chaloin O,Hartley O,Offord R,Mosier DE

更新日期：2001-01-01 00:00:00

abstract：:Identification of cell surface viral binding proteins is important for understanding viral attachment and internalization. We have fused the pre-S domain of the duck hepatitis B virus (DHBV) large envelope protein to glutathione S-transferase and demonstrated a 170-kDa binding protein (p170) in [35S]methionine-labeled...

journal_title：Journal of virology

authors： Tong S,Li J,Wands JR

更新日期：1995-11-01 00:00:00

abstract：:Two regions of the hepatitis B virus (HBV) genome have been shown to display properties of a transcriptional enhancer. Enhancer 1 is active in most hepatoma lines examined as well as in some non-hepatocyte-derived cell lines. In contrast, enhancer 2 activity is strictly liver specific. In this study, we show that aden...

journal_title：Journal of virology

authors： Chen ST,Su H,Yee JK

更新日期：1992-12-01 00:00:00

abstract：:A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...

journal_title：Journal of virology

authors： Steele KP,Frist RH

更新日期：1978-05-01 00:00:00