Abstract:
:Establishing reference ranges by multiparametric immunophenotyping of mature B cells in bone marrow of healthy adults is of interest because the detection of bone marrow infiltration and persistence of light chain restriction, as well as discrimination between reactive and malignant lymphocytes are important applications of B-cell immunophenotyping. To determine the pattern of antigens as expressed by malignant mature B lymphocytes in the present study, bone marrow aspirates of healthy adults were investigated for the presence and percentage frequency of those antigens as defined for immunophenotyping of B cells by the REAL Classification. For this purpose, analysis of CD19-positive B lymphocytes by 'live gate' analysis was performed. The established two-color as well as three-color stainings will serve as a basis for future investigations designed to test multiparametric analysis of B lymphocytes in bone marrow aspirates. All investigated antibodies stained with varying percentage frequency on B-cell subtypes, and no statistical significant difference was found between bone marrow aspirates of women and those of men. On the basis of this analysis, all the reported lineage antigen combinations are present both in malignant B lymphocytes and in normal B cells in considerable percentage frequency. These findings are of importance for follow-up investigations of patients with non-Hodgkin's lymphomas by multiparametric immunophenotyping.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Höffkes HG,Schmidtke G,Schmücker U,Uppenkamp M,Brittinger Gdoi
10.1007/BF01702647subject
Has Abstractpub_date
1995-09-01 00:00:00pages
123-8issue
3eissn
0939-5555issn
1432-0584journal_volume
71pub_type
杂志文章abstract::Indolent primary cutaneous B cell lymphomas (PCBCL) generally have a good prognosis, but they often relapse leading in some cases to extracutaneous disease and therefore, to poor survival. We developed a prognostic model to improve the therapeutic approach to these lymphomas. Two hundred and seventeen patients with di...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-010-1083-1
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abstract::The objective of this study was to investigate whether metabolic tumor volume (MTV) by positron emission tomography (PET) can be a potential prognostic tool when compared with Ann Arbor stage, in stages II and III nodal diffuse large B cell lymphoma (DLBCL). We evaluated 169 patients with nodal stages II and III DLBCL...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1357-2
更新日期:2012-05-01 00:00:00
abstract::Evaluation of bone marrow involvement (BMI) by conventional bone marrow biopsy (BMB) can generate false-negative results if marrow disease is focal. The sensitivity of 18F-2-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (FDG-PET/CT) in assessing BMI in extranodal NK/T cell lymphoma (ENKL) h...
journal_title:Annals of hematology
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doi:10.1007/s00277-014-2289-4
更新日期:2015-06-01 00:00:00
abstract::Cryoglobulinemia is uncommon in Southern Chinese in Hong Kong, with tropical climates and low incidence of hepatitis C virus (HCV) infection. Eight positive cases were detected among 481 patients screened for cryoglobulins over a 10-year period. Three HCV carriers (38%) ran benign courses. The others included two carr...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-004-0955-7
更新日期:2005-02-01 00:00:00
abstract::An acute leukemia with an unusual immunophenotype developed in a 17-year-old girl. At the initial presentation, extramedullary involvement was not evident, but with advancing disease, massive splenomegaly and an osteolytic rib tumor developed. The disease was aggressive and refractory to intensive chemotherapeutic reg...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050472
更新日期:1999-01-01 00:00:00
abstract::Gastrointestinal graft-versus-host disease (GVHD) is one of the main causes of therapy-related death after allogeneic hemopoietic stem cell transplantation. Early diagnosis and immediate treatment are probably essential for improving clinical outcome. High-resolution sonography allows the evaluation of single bowel wa...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-004-0893-4
更新日期:2005-01-01 00:00:00
abstract::The current phase-II trial was initiated to assess the efficacy and toxicity of the Ida-FLAG regimen in patients with poor-risk acute myeloid leukemia (AML). Three subgroups of patients with AML were eligible for the study: (a) refractory, (b) first relapse, or (c) secondary AML (i.e., signs of trilineage myelodysplas...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1007/s002770050541
更新日期:1999-09-01 00:00:00
abstract::To investigate whether cytokine genetic polymorphisms influence the outcome of diffuse large B cell lymphoma (DLBCL), we tested 337 consecutive DLBCL treated with CHOP or rituximab-CHOP (R-CHOP) from interleukin 10 (IL10), Bcl-2, and tumor necrosis factor (TNF)-α polymorphisms. Patients who carried the IL10 rs1800871 ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2857-x
更新日期:2017-02-01 00:00:00
abstract::Cancer incidence in patients with recurrent unprovoked venous thromboembolism (VTE) is much higher than after a first event, but the incidence of myeloproliferative neoplasms (MPN) in this situation is still unknown. We tested for JAK2V617F and calreticulin mutants, 372 DNA samples of patients treated for (VTR). Among...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2853-1
更新日期:2017-03-01 00:00:00
abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hemolytic disease with thrombosis as a major complication. The mechanism of thrombosis and related risk factors in PNH patients are still not well characterized. We retrospectively enrolled 99 patients with newly diagnosed PNH at our institute from 2011 to 2016....
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-019-03770-3
更新日期:2019-10-01 00:00:00
abstract::Imatinib is a new promising therapeutic option for chronic myeloid leukemia (CML) with efficacy even in the blast phase of the disease. However, most patients treated with imatinib in the blast phase develop progressive disease rapidly. Thus, treatment with imatinib has to be followed by other treatment strategies. Th...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0643-z
更新日期:2003-05-01 00:00:00
abstract::In the present study, we questioned whether the cholesterol synthesis inhibitor lovastatin potentiates the cytotoxicity of chemotherapeutic agents in the primitive CD34(+) subpopulation of acute myeloid leukemia (AML) cells. AML mononuclear cells (n = 17) were sorted in CD34(+) and CD34(-) fractions and compared to no...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-008-0633-2
更新日期:2009-06-01 00:00:00
abstract::The appropriate management of patients with moderate aplastic anemia (mAA) remains to be unclear and controversial. A cohort of 118 patients with mAA received a novel immunosuppressive strategy of cyclosporine alternately combined with levamisole (CSA and LMS regimen), which included 42 newly diagnosed and 76 chronic ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-013-1764-7
更新日期:2013-09-01 00:00:00
abstract::Polo-like kinase 2 (SNK/PLK2), a transcriptional target for wild-type p53 and is hypermethylated in a high percentage of multiple myeloma and B cell lymphomas patients. Given these data, we sought to study the methylation status of the specific gene in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), a...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1193-4
更新日期:2011-09-01 00:00:00
abstract::Acquired aplastic anemia (AA) and paroxysmal nocturnal hemoglobinuria (PNH) are interrelated ultra-rare diseases. Quality of life (QoL) evaluation tools used in studies for AA and PNH are unspecific and designed for cancer patients (e.g., the European Organization for Research and Treatment of Cancer Quality of Life Q...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2867-8
更新日期:2017-02-01 00:00:00
abstract::Multiple myeloma is a malignant B-cell neoplasm that involves the skeleton in approximately 80% of the patients. With an average age of 60 years and a 5-years survival of nearly 45% Brenner et al. (Blood 111:2516-2520,35) the onset is to be classified as occurring still early in life while the disease can be very aggr...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-009-0829-0
更新日期:2009-12-01 00:00:00
abstract::Extreme thrombocytosis (ExT) has been associated with an increased bleeding risk in myeloproliferative neoplasm (MPN) patients and is included in the high risk category in treatment guidelines. Treatment of patients with ExT has not been studied in prospective trials. To study physicians' approaches to ExT, we distrib...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2826-4
更新日期:2017-01-01 00:00:00
abstract::Thrombotic thrombocytopenia purpura (TTP) and atypical hemolytic uremic syndromes (aHUS) are distinct clinical disorders characterized by hemolytic anemia, thrombocytopenia, microthrombi, and end organ damage. TTP is characterized by a low ADAMTS13 activity level at diagnosis of <10 % ADAMTS13 activity, while aHUS is ...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-015-2411-2
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abstract::Secondary central nervous system (CNS) involvement in diffuse large B-cell lymphoma (DLBCL) includes an isolated CNS relapse or CNS involvement with systemic disease progression. This rare but fatal clinical problem still remains a therapeutic dilemma in the management of DLBCL. However, there are limited data about i...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-010-1104-0
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究,随机对照试验
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abstract::Parainfluenza virus (PIV) infection is a significant cause of morbidity and mortality, especially in hematologic malignancy patients including hematopoietic stem cell transplantation (HCT) recipients. However, limited information is available for risk stratification in PIV-infected patients with hematologic malignancy...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-020-03996-6
更新日期:2020-06-01 00:00:00
abstract::Alterations in hemoglobin oxygen affinity can be detected by exposing blood to different PO2 and recording oxygen saturation, a method termed tonometry. It is the gold standard to measure the PO2 associated with 50 % oxygen saturation, the index used to quantify oxygen affinity (P50Tono). P50Tono is used in the evalua...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-012-1667-z
更新日期:2013-04-01 00:00:00
abstract::The benefits of intensive post-remission chemotherapy have not been verified in elderly patients with acute myeloid leukemia (AML). To reduce fatal complications caused by intensive post-remission therapy, we performed a prospective phase II multicenter trial of standard induction chemotherapy ('7+3' of cytarabine plu...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-006-0110-8
更新日期:2006-06-01 00:00:00
abstract::While inherited X-linked (XL) isolated thrombocytopenia is a mild condition, the Wiskott-Aldrich syndrome (WAS) associates severe thrombocytopenia with an immunodeficiency component and has a poor prognosis. Whether these conditions correspond to separate genetic entities or to different mutations of the same gene(s) ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01707282
更新日期:1991-08-01 00:00:00
abstract::We screened for increased osmotic fragility of erythrocytes in 1464 healthy German blood donors. The osmotic fragility was determined by an acidified glycerol lysis test (AGLT) using glycerol-sodium phosphate-buffered NaCl solution. Since the original test described by Zanella et al. [23] showed only low specificity f...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01715351
更新日期:1992-02-01 00:00:00
abstract::The efficacy of a single dose of MCNU 150 mg was evaluated in nine symptomatic patients with essential thrombocythemia (ET). As the platelet counts increased during the following 4 weeks, an extra dose of 100 mg of MCNU was administered to three patients. All patients had thrombotic or hemorrhagic complications. Seven...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01738474
更新日期:1993-05-01 00:00:00
abstract::2-Chlorodeoxyadenosine (2-CdA) is a purine analogue which has proved to be active in acute myeloid leukemia (AML), especially in children. In adults, results yielded by 2-CdA alone or with ara-C were less encouraging. Here we report on the efficacy of 2-CdA with or without daunorubicin (DNR) in 19 relapsing or refract...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s002770050355
更新日期:1998-01-01 00:00:00
abstract::Infections and especially blood stream infections (BSI) with gram-negative bacteria (GNB) represent a major threat for patients with hematological diseases undergoing chemotherapy and mainly contribute to morbidity and mortality. In this retrospective single-center study, we analyzed the impact of BSI with different g...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-018-3423-5
更新日期:2018-11-01 00:00:00
abstract::Patients with malignancy often present with a variety of coagulation abnormalities which may ultimately lead to recurrent arterial and venous thromboses. Recently the presence of antiphospholipid antibodies in cancer patients has been proposed as one of the potential mechanisms promoting hypercoagulability. Here we re...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0565-1
更新日期:2002-12-01 00:00:00
abstract::The Infectious Diseases Working Party of the German Society of Haematology and Oncology presents their guidelines for the treatment of fungal infections in patients with hematological and oncological malignancies. These guidelines are evidence-based, considering study results, case reports and expert opinions, using t...
journal_title:Annals of hematology
pub_type: 指南,杂志文章,实务指引
doi:10.1007/s00277-003-0767-1
更新日期:2003-10-01 00:00:00