Abstract:
:Intracellular protein degradation is one of the most precisely regulated processes in living cells. The main component of the degradation machinery is the 20S proteasome present in eukaryotes as well as in prokaryotes. We have developed successful purification protocols for the 20S proteasome in its native state using an affinity tag strategy. This chapter describes in detail the purification protocols, proteolytic activity assays, crystallization, and structure determination for the yeast 20S proteasome. The crystal structure of the eukaryotic proteasome opens new possibilities for identifying, characterizing, and elucidating the mode of action for natural and synthetic inhibitors, which affect its function. Some of these compounds may find therapeutic applications in contemporary medicine.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Groll M,Huber Rdoi
10.1016/S0076-6879(05)98027-0subject
Has Abstractpub_date
2005-01-01 00:00:00pages
329-36eissn
0076-6879issn
1557-7988pii
S0076-6879(05)98027-0journal_volume
398pub_type
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journal_title:Methods in enzymology
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abstract::Vertebrate odorant-binding proteins (OBPs) are small soluble proteins abundantly secreted in the olfactory mucus of many animal species, including humans. Vertebrate OBPs reversibly bind odorant molecules with micromolar range affinities. Although their physiological role is not clearly understood, OBPs are proposed t...
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