[Polymerase chain reaction and its application].

Abstract:

:The polymerase chain reaction (PCR) is one of the most important new methods in molecular biology. It is widely used in genetic and anthropologic basic research, in oncology and virology, in all those fields, where molecular biologic methods can give answers to the questions raised. The procedure enables one to multiply with extreme precision targeted pieces of amounts as little as one target molecule of DNA or RNA by five to six logs, making them easy to be handled and examined by routine molecular biological methods. The method is presented through one possible application field, that is of great importance in the study of hepatocarcinogenesis. Sensitivity of PCR in detection of hepatitis B virus DNA is greater by four logs than animal inoculation, the last most sensitive method known.

journal_name

Orv Hetil

journal_title

Orvosi hetilap

authors

Sárosi I,Gerald E,Girish VN

subject

Has Abstract

pub_date

1992-07-05 00:00:00

pages

16-21

eissn

0030-6002

issn

1788-6120

journal_volume

133 Suppl 1

pub_type

杂志文章,评审
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  • [Incidence and elimination of false-negative results of ferritin determination].

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    authors: Papik K,Molnár B,Fedorcsák P,Rainer S,Fridl L,Fehér J,Tulassay Z

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  • [Evaluation of the teratogenic effects of occupational hazards during pregnancy].

    abstract::The Hungarian Case-Control Surveillance System of Congenital Anomalies, 1980-1987 contains 10,937 index patients affected by congenital anomalies and 10,937 matched control cases. Their comparative analysis indicated a significantly higher rate of some congenital anomaly types in the offspring of women worked in agric...

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    authors: Király J,Czeizel E

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  • [Drug therapy of gastroesophageal reflux (a prospective controlled clinical trial)].

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    doi:

    authors: Wittmann T,Rosztóczy A,Fehér A,Jármay K,Oláh T,Szendrényi V,Kiss I

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  • [Leukocyte migration inhibition test in celiac disease].

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    doi:

    authors: Horváth K,Horn G,Walcz E,Zavodni I,Bodánszky H

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  • [Relationship between intestinal permeability and antibodies against food antigens in IgA nephropathy].

    abstract::Elevated antibody titers to food antigens and increased intestinal permeability in IgA nephropathy (IgA NP) suggest that these factors may be interdependent and play a role in the pathogenesis of the disease. In 1989 IgA and IgG antibodies against eight common food antigens were examined in the sera of 35 IgA NP patie...

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    pub_type: 临床试验,杂志文章,评审

    doi:

    authors: Kovács T,Mette H,Per B,Kun L,Schmelczer M,Barta J,Jean-Claude D,Nagy J

    更新日期:1996-01-14 00:00:00

  • [Diagnosis and management of endocrine crises in adulthood].

    abstract::Diagnosis and management of endocrine crises in adulthood. The homeostasis of the body is regulated by concerted action of the endocrine, nervous and immune systems. An imbalance in this equilibrium, as a consequence of disease or endocrine failure, may lead to crisis, characterized by sudden onset and severe symptoms...

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    pub_type: 杂志文章,评审

    doi:

    authors: Vadász J

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  • [Whipple's disease. Do we think of it enough?].

    abstract:UNLABELLED:Whipple's disease is a chronic, systemic, relapsing bacterial illness, which is always fatal without treatment, and means a diagnostic challenge for both clinicians and pathologists. It occurs in Caucasian, white, middle-aged men in 80 % of the cases. CASE REPORT:The authors present three cases, and review ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/OH.2009.28668

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    更新日期:2009-07-12 00:00:00

  • [High-resolution computer tomography of diffuse infiltrative lung diseases].

    abstract::During a period of 17 months, a total of 101 patients were examined by high resolution computed tomography (HRCT) whose minor focal changes revealed by chest X-ray raised the suspicion of diffuse infiltrative pulmonary disease. The disease of the interstitium was primarily caused by idiopathic pulmonary fibrosis (19%)...

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    doi:

    authors: Vajda E,Mester A,Major T,Kiss K,Appel J,Magyar P,Makó E

    更新日期:1998-12-27 00:00:00

  • [Gut-liver axis in inflammatory bowel disease. A retrospective study].

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  • [Role of somatostatin receptor ligands in the treatment of acromegaly--literature review].

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  • [Successful operation of bleeding perforated Curling's ulcer].

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  • [Rickettsia helvetica: an emerging tick-borne pathogen in Hungary and Europe].

    abstract::Rickettsia helvetica belonging to spotted fever group rickettsiae was recently detected by polymerase chain reaction followed by sequencing in European sheep ticks (Ixodes ricinus) from Hungary. Current knowledge on these rickettsiae and the clinical and diagnostic aspects of R. helvetica infection is summarized. In a...

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    doi:

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  • [Correlation between ABO and Rh blood groups, serum cholesterol and ischemic heart disease in patients undergoing coronarography].

    abstract:UNLABELLED:The associations of the ABO- and Rh-blood groups, the serum cholesterol and the extent of the coronary artery disease were examined in 653 patients who underwent coronary angiography between 1980 and 1985 in the Hungarian Institute of Cardiology. CONCLUSIONS:1. among the patients with positive coronarograph...

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    authors: Tarján Z,Tonelli M,Duba J,Zorándi A

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  • [The prognostic value of cytokeratin-18 cell death marker in cardiac arrest survivors].

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  • [Bicentennial of catalase research, 1818-2018].

    abstract::L. J. Thénard and J. L. Gay-Lussac discovered hydrogen peroxide in 1818. Later, Thénard noticed that animal and plant tissues decompose hydrogen peroxide. The substance which is responsible for this reaction was named as catalase by O. Loew in 1900. The catalase enzyme was regarded as a diagnostic and a tumour marker ...

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    pub_type: 历史文章,杂志文章

    doi:10.1556/650.2018.31096

    authors: Góth L

    更新日期:2018-06-01 00:00:00

  • [Epidemiology, risk factors and molecular pathogenesis of primary liver cancer].

    abstract::Primary liver cancer is the fifth most common cancer worldwide. Hepatocellular carcinoma accounts for 85-90% of primary liver cancers. Distribution of hepatocellular carcinoma shows variations among geographic regions and ethnic groups. Males have higher liver cancer rates than females. Hepatocellular carcinoma occurs...

    journal_title:Orvosi hetilap

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    doi:10.1556/OH.2008.28313

    authors: Hagymási K,Tulassay Z

    更新日期:2008-03-23 00:00:00

  • [Nephrogenic diabetes insipidus].

    abstract::The syndrome of polyuria and polydipsia is practically identical in three very different disorders (central diabetes insipidus, nephrogenic diabetes insipidus, primary polydipsia). In congenital nephrogenic diabetes insipidus both the thirst mechanism and the production of the antidiuretic hormone are intact, but the ...

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    doi:

    authors: Radó J

    更新日期:1998-03-08 00:00:00

  • [Limitations and possibilities of detecting lung cancer by fluoroscopic screening of the population].

    abstract::In 10th district of Budapest a longitudinal, epidemiological examination was started in 1975 with the aim of analysis of the objective factors influencing the population screening and determination of possibilities of screening effectiveness. It was found that effectiveness of lung cancer detection with screening depe...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Abrahám E,Karácsonyi L,Dinya E

    更新日期:1989-07-30 00:00:00

  • [Surgical treatment of pulmonary metastases from colorectal tumors].

    abstract::Authors analysed 25 colorectal cancer lung metastases. Among them were 13 male 12 female. Ages ranged from 45 to 70 years. The lung nodules were discovered without any symptoms in 19 cases. The disease was localized on the right in 16 patients, in 7 on the left side and 2 others were bilateral. There were 19 solitary ...

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    pub_type: 杂志文章

    doi:

    authors: Csekeö A,Orbán K,Agócs L,Pálffy G

    更新日期:1995-01-29 00:00:00

  • [Image-guided radiotherapy for muscle invasive bladder cancer with intravesical lipiodol injection. A new option for bladder sparing treatment].

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  • [Urinary tract infections in diabetes mellitus].

    abstract::Urinary tract infections are more frequent in diabetic patients than in non-diabetics, or take a more severe course. The difference is more pronounced in women both in symptomatic infections and asymptomatic bacteriuria. The spectrum of pathogens is similar to that of non-diabetic patients. In case of cystitis, the tr...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2008.28298

    authors: Ludwig E

    更新日期:2008-03-30 00:00:00

  • [Clinical proarrhythmias induced by anti-arrhythmic drugs, non-cardiovascular agents and implantable cardioverter-defibrillators].

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    doi:

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  • [Trends and current questions of cardiovascular prevention in primary health care].

    abstract::Although an impressive progress has been achieved in the treatment of cardiovascular diseases, they are at the top of the mortality statistics in Hungary. Prevention of these diseases is an essential task of the primary health care. Cardiovascular prevention is carried out at primary, secondary and tertiary levels usi...

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  • [Atypical course of Kawasaki syndrome in a 3-month old infant].

    abstract::The authors report on the case history of a three-month-old infant with Kawasaki-syndrome. The course of the disease was atypical, the various symptoms (antibiotic resistant high fever, urticariform rash, meningitis serosa, steril pyuria, coronaria aneurysma, thrombocytosis, high sedimentation rate) developed slowly d...

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    doi:

    authors: Teufel A,Szauer E

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  • Molecular underpinnings of systemic treatment resistance in metastatic castration-resistant prostate cancer

    abstract::In the last few years, several new drugs with various mechanisms of action have been approved for the treatment of castration-resistant prostate cancer. Due to this development, therapeutic decision-making has become increasingly complex. Therefore, therapy selection as well as timing and sequence of treatments need t...

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    pub_type: 杂志文章,评审

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  • [Pacemaker in MR: absolute contraindication?].

    abstract::Due to developments in pacemaker technology, implanted pacemakers do not mean an absolute contraindication for MRI examination. However, there are several aspects of MRI examinations that should be considered for safety reasons in pacemaker patients. Based on literature data and own experiments, the safety protocol of...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/OH.2008.28122

    authors: Bogner P,Tóth L,Simor T,Gulyás J,Lukács G,Papp L,Repa I

    更新日期:2008-03-09 00:00:00

  • [May-Thurner syndrome].

    abstract::May-Thurner syndrome, a pelvic vein congenital anomaly is the consequence of compression of the left common iliacal vein by the overlying right common iliacal artery that results in an increased risk for ileofemoral deep vein thrombosis. Authors present a case of a young female with aberration of the pelvic vein, who ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/OH.2011.28998

    authors: Kecskeméthy Z,Holý J

    更新日期:2011-01-23 00:00:00

  • [Side effect of pegylated-interferon treatment in chronic C hepatitis: agranulocytosis].

    abstract::Chronic C hepatitis is a global health problem. Its treatment is still unresolved. Pegylated interferon means substantive breakthrough in therapy. The longer effect, the lasting, steady therapeutic blood level are the pharmacokinetic advances. There is no significant difference in the side effects of pegylated interfe...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Halász T,Farkas A,Tolvaj G,Horváth G

    更新日期:2006-02-19 00:00:00

  • [Total mesorectal excision with ultrasonic coagulation knife ("UltraCision") in surgery of rectal cancer].

    abstract::The authors report a total of 62 middle and low third rectal cancer cases operated on by total mesorectal excision by the method of Heald. The oncological basis of this procedure is the horizontal regional metastatization of rectal cancer. The total mesorectal excision facilitates, the low anterior resections and pres...

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    pub_type: 杂志文章

    doi:

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    更新日期:2000-02-20 00:00:00