Abstract:
:Several proteins involved in exocytosis have been identified recently, but it is still completely unclear which molecules perform the fusion event itself. Although in viral fusion the fusion proteins are known, even there the molecular mechanism remains controversial. Investigation of single fusion events by electrophysiological techniques together with fluorimetric measurements have now provided some insight into the properties of the first aqueous connection, the fusion pore. This pore has an initial size similar to an ion channel and allows movement of lipids only after it has substantially expanded, indicating that it is initially not a purely lipidic structure, but incorporates lipids when it expands. Although neurotransmitter release may occur through narrow transient fusion pores, the fusion pore of synaptic vesicles probably expands vey rapidly, making it unlikely that secretion is performed by rapid exo/endocytosis without full fusion under normal conditions. Recent recordings from small membrane patches have made it possible to resolve fusion events from vesicles as small as synaptic vesicles. Future experiments using excised patches may provide an approach to identify the molecular machinery of exocytotic membrane fusion.
journal_name
Curr Opin Cell Bioljournal_title
Current opinion in cell biologyauthors
Lindau M,Almers Wdoi
10.1016/0955-0674(95)80007-7subject
Has Abstractpub_date
1995-08-01 00:00:00pages
509-17issue
4eissn
0955-0674issn
1879-0410pii
0955-0674(95)80007-7journal_volume
7pub_type
杂志文章,评审abstract::It is during gastrulation that the primordial germ layers are specified, embryonic axes become morphologically manifest, and the embryonic body plan begins to take shape. As morphogenetic movements push and pull nascent tissues into position within the gastrula, new interactions are established between neighboring cel...
journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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abstract::Considerable advances into the basis of RNA-polymerase-II-mediated transcriptional regulation have recently emerged. Biochemical, genetic and structural studies have contributed to novel insights into transcription, as well as the functional significance of covalent histone modifications. New details regarding transcr...
journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in cell biology
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abstract::Several recently discovered mammalian cell adhesion proteins recognize and bind to sialic acid-containing ligands. Reports concerning the molecular specificities of these interactions have been intriguing but somewhat confusing, partly because of pitfalls in methodology or interpretation. Nevertheless, these protein-c...
journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:1995-10-01 00:00:00
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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更新日期:1994-10-01 00:00:00
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journal_title:Current opinion in cell biology
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doi:10.1016/j.ceb.2008.06.007
更新日期:2008-10-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:2012-02-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:2013-02-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:1993-06-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:1997-02-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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更新日期:1993-04-01 00:00:00
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journal_title:Current opinion in cell biology
pub_type: 杂志文章,评审
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