Determination of beta-choriogonadotropin in human plasma; evaluation and comparison of five "kit" methods.

abstract：PURPOSE:To review information on the use of laboratory tests in screening, diagnosis, and monitoring of acute and chronic hepatic injury. DATA SOURCES AND STUDY SELECTION:A MEDLINE search was performed for key words related to hepatic diseases, including acute hepatitis, chronic hepatitis, alcoholic hepatitis, cirrhos...

journal_title：Clinical chemistry

authors： Dufour DR,Lott JA,Nolte FS,Gretch DR,Koff RS,Seeff LB

更新日期：2000-12-01 00:00:00

abstract：BACKGROUND:Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal geneti...

journal_title：Clinical chemistry

authors： Li Y,Zimmermann B,Rusterholz C,Kang A,Holzgreve W,Hahn S

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

journal_title：Clinical chemistry

authors： Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

更新日期：2004-02-01 00:00:00

abstract：:The characteristics of the Abbott selective fluorescence polarization immunoassay for cyclosporine in whole blood (TDXSP) were compared with the Incstar radioimmunoassay by using kit standards (INCSP) or TDXSP standards (INCTDX) with samples from adult (48) and pediatric (24) liver-transplant recipients and a further ...

journal_title：Clinical chemistry

authors： Tredger JM,Gonde CE,Williams R

更新日期：1992-01-01 00:00:00

abstract：:We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working li...

journal_title：Clinical chemistry

authors： Tomokuni K,Ichiba M,Hirai Y,Hasegawa T

更新日期：1987-09-01 00:00:00

abstract：:Macromolecular aspartate aminotransferase was found in the serum of an apparently healthy patient. This complex was composed of aspartate aminotransferase (AST; EC 2.6.1.1) and immunoglobulin. Electrophoresis of the patient's serum showed an abnormal band migrating between mitochondrial (m) and cytosolic (s) AST. The ...

journal_title：Clinical chemistry

authors： Stasia MJ,Surla A,Renversez JC,Pene F,Morel-Femelez A,Morel F

更新日期：1994-07-01 00:00:00

abstract：BACKGROUND:Current noninvasive assays for urothelial carcinoma (UC) lack clinical sensitivity and specificity. Given the utility of plasma cell-free DNA (cfDNA) biomarkers, the development of urinary cfDNA biomarkers may improve the diagnostic sensitivity. METHODS:We assessed copy number alterations (CNAs) by shallow ...

journal_title：Clinical chemistry

authors： Ge G,Peng D,Guan B,Zhou Y,Gong Y,Shi Y,Hao X,Xu Z,Qi J,Lu H,Zhang X,Zhan Y,Li Y,Wu Y,Ding G,Shen Q,He Q,Li X,Zhou L,Ci W

更新日期：2020-01-01 00:00:00

abstract：:We have compared the operational, medical, and fiscal consequences of performing urgent glucose testing at the bedside vs in the central laboratory. The turnaround time (TAT) with bedside testing was only 1-2 min shorter than that from the central laboratory, to which specimens are sent by pneumatic tube and from whic...

journal_title：Clinical chemistry

authors： Winkelman JW,Wybenga DR,Tanasijevic MJ

更新日期：1994-08-01 00:00:00

abstract：:We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all th...

journal_title：Clinical chemistry

authors： Maitra SK,Yoshikawa TT,Hansen JL,Nilsson-Ehle I,Palin WJ,Schotz MC,Guze LB

更新日期：1977-12-01 00:00:00

abstract：BACKGROUND:Biochemical markers of bone turnover can provide prognostic information about the risk of osteoporotic fracture and are useful tools for monitoring efficacy of antiresorptive therapy. A serum-based automated assay may be of better clinical value than urinary markers because of lower imprecision and day-to-da...

journal_title：Clinical chemistry

authors： Garnero P,Borel O,Delmas PD

更新日期：2001-04-01 00:00:00

abstract：:In this report on trace-element concentrations (As, Ca, Cd, Cl, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Rb, Sb, Se, Zn) in human heart, liver, kidney, aorta, and rib obtained from 200 autopsied patients, we give special attention to sampling procedure, analysis technique, and various sources of error (autolysis...

journal_title：Clinical chemistry

authors： Aalbers TG,Houtman JP,Makkink B

更新日期：1987-11-01 00:00:00

abstract：:This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived ...

journal_title：Clinical chemistry

authors： MacDonald DJ,Mehta HC,Mack DS,Duffy T,Glen AC

更新日期：1984-11-01 00:00:00

abstract：:This assay was developed to measure apolipoprotein (apo) B in blood samples spotted onto filter paper. The long-term aim is to detect young families with the dominantly inherited familial hypercholesterolemia, during current neonatal screening programs. The interassay CV was 5.6%, and apo B concentrations correlated c...

journal_title：Clinical chemistry

authors： Wang XL,Dudman NP,Wilcken DE

更新日期：1989-06-01 00:00:00

abstract：BACKGROUND:Concentrations of physiological response variables fluctuate over time. The present study describes within-day and seasonal fluctuations for total cholesterol, dehydroepiandrosterone sulfate (DHEA-S), hemoglobin A(1c) (HbA(1c)), IgA, prolactin, and free testosterone in blood, and estimates within- (CV(i)) an...

journal_title：Clinical chemistry

authors： Garde AH,Hansen AM,Skovgaard LT,Christensen JM

更新日期：2000-04-01 00:00:00

abstract：:We used enzyme immunoassay to measure apolipoprotein B concentration in human plasma. Pure lipoprotein B was isolated from serum samples of fasting normolipidemic subjects by sequential preparative ultracentrifugation and coated to a polystyrene tube surface by adsorption. Human serum samples and rabbit antiserum to h...

journal_title：Clinical chemistry

authors： Fruchart JC,Desreumaux C,Dewailly P,Sezille G,Jaillard J,Carlier Y,Bout D,Capron A

更新日期：1978-03-01 00:00:00

abstract：BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD...

journal_title：Clinical chemistry

authors： Kishi K,Ochiai K,Ohta Y,Uemura Y,Kanatani K,Nakajima K,Nakamura M

更新日期：2002-05-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：:We evaluated a new agarose-gel-electrophoretic procedure (Corning) (I) for separating and quantitating of high-density lipoprotein cholesterol (HDLC), comparing it with the modified Lipid Research Clinics (LRC) procedure (heparin 183 kilounits/L, MnCl2 92 mmol/L) (II). Method I was insensitive to an HDLC concentration...

journal_title：Clinical chemistry

authors： Stein EA,McNeely S,Steiner P

更新日期：1979-11-01 00:00:00

abstract：:A new instrument has been developed that is similar in design and concept to the GeMSAEC centrifugal analyzer (Oak Ridge National Laboratory) except that electrochemical detection is used rather than optical detection. The present version has eight channels, each with its own detector. Problems of speed control and sa...

journal_title：Clinical chemistry

authors： Cho HK,Lee YH,Couch RA,Jagadeesh JM,Olson CL

更新日期：1982-09-01 00:00:00

abstract：BACKGROUND:Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary refere...

journal_title：Clinical chemistry

authors： van der Vorm LN,Hendriks JC,Laarakkers CM,Klaver S,Armitage AE,Bamberg A,Geurts-Moespot AJ,Girelli D,Herkert M,Itkonen O,Konrad RJ,Tomosugi N,Westerman M,Bansal SS,Campostrini N,Drakesmith H,Fillet M,Olbina G,Pasricha

更新日期：2016-07-01 00:00:00

abstract：:Using image-analyzing equipment, we measured the effect of quinidine on the activity of naphthol AS-D chloroacetate esterase, alpha-naphthyl butyrate esterase, alpha-naphthyl acetate esterase, and acid phosphatase in individual cells of a human Hep G2 hepatoma cell line. The impact of the drug on the morphology of the...

journal_title：Clinical chemistry

authors： Markovic OT,Young DS,Markovic NS

更新日期：1988-03-01 00:00:00

abstract：:A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma. The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.5 mol/L), which is used as the sample for the sp...

journal_title：Clinical chemistry

authors： Bourdon S,Houzé P,Bourdon R

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND:Protein tyrosine phosphatase 1B (PTPN1) dephosphorylates insulin receptors and attenuates insulin signaling. Polymorphisms in the coding sequence of PTPN1 have been variably associated with type 2 diabetes (T2D). We hypothesized that variations within the PTPN1 promoter might contribute to the development of...

journal_title：Clinical chemistry

authors： Meshkani R,Taghikhani M,Al-Kateb H,Larijani B,Khatami S,Sidiropoulos GK,Hegele RA,Adeli K

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:In vitro and animal studies suggest that tumor necrosis factor alpha (TNF-alpha) modulates intestinal iron transport. We hypothesized that the effect of TNF-alpha might be particularly relevant if iron absorption is not effectively controlled by the HFE gene. METHODS:In patients with homozygous C282Y hemoch...

journal_title：Clinical chemistry

authors： Krayenbuehl PA,Maly FE,Hersberger M,Wiesli P,Himmelmann A,Eid K,Greminger P,Vetter W,Schulthess G

更新日期：2006-08-01 00:00:00

abstract：:Ethchlorvynol is a commonly abused sedative hypnotic. I report here a seven-day study of serum ethchlorvynol concentrations in a patient who had received an extreme overdose. These concentrations were correlated with the patient's response to stimuli. Serum ethchlorvynol concentrations were logarithmically related to ...

journal_title：Clinical chemistry

authors： Pochopien DJ

更新日期：1975-06-01 00:00:00

abstract：:Lyophilized serum specimens were manufactured in such a way that fixed quantitative interspecimen relationships were established. The specimens were analyzed for about twenty constituents by several thousand laboratories in 1971, 1972, and 1973. Most of the consensus mean values derived from these analyses were found ...

journal_title：Clinical chemistry

authors： Grannis GF

更新日期：1976-07-01 00:00:00

abstract：BACKGROUND:Increasing numbers of patients are presenting worldwide to emergency departments with suspected myocardial infarction. The use of point-of-care troponin assays might enable faster decision-making in this high-risk population and reduce the burden on emergency facilities. Here, we evaluate the diagnostic perf...

journal_title：Clinical chemistry

authors： Sörensen NA,Neumann JT,Ojeda F,Giannitsis E,Spanuth E,Blankenberg S,Westermann D,Zeller T

更新日期：2019-12-01 00:00:00

abstract：:We present the results of measurements of alkaline phosphatase activity from interlaboratory transferability studies conducted by 12 laboratories in five countries. The variability, as demonstrated by within-day precision (CV less than or equal to 2.6%), between-day precision (CV less than or equal to 3.6%), and betwe...

journal_title：Clinical chemistry

authors： Tietz NW,Rinker AD,Burtis C,Duncan P,Ervin K,Ewen L,Hørder M,Mathieu M,Petitclerc C,Grisley D

更新日期：1984-05-01 00:00:00

abstract：BACKGROUND:Immune repertoire sequencing of the T-cell receptor can identify clonotypes that have expanded as a result of antigen recognition or hematological malignancies. However, current sequencing protocols display limitations with nonuniform amplification and polymerase-induced errors during sequencing. Here, we de...

journal_title：Clinical chemistry

authors： Johansson G,Kaltak M,Rîmniceanu C,Singh AK,Lycke J,Malmeström C,Hühn M,Vaarala O,Cardell S,Ståhlberg A

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small cell lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for lung cancer diagnosis and subtyping. Yet, histologic distinction of...

journal_title：Clinical chemistry

authors： Bensussan AV,Lin J,Guo C,Katz R,Krishnamurthy S,Cressman E,Eberlin LS

更新日期：2020-11-01 00:00:00