Influence of middle molecules on the anemia of uremic patients.

Abstract:

:To evaluate their toxicity at the cellular level, middle molecules from uremic serum were incubated with erythrocytes from healthy subjects and the activity of the enzyme Delta-aminolevulinic acid dehydrase (D-ALA-D) and peroxidative hemolysis were investigated. Uremic middle molecules caused a significant decrease of the D-ALA-D activity of normal erythrocytes which was not due to differences in the concentrations of Pb, Cd or Zn. The decreased enzyme activity could be restored by adding reduced glutathione (GSH; 5 mmol/L) together with the middle molecules to the assay system. Uremic middle molecules caused a significant increase of peroxidative hemolysis in normal erythrocytes. Uremic middle molecules contribute to the anemia of uremic patients by impeding hemoglobin synthesis and by increasing peroxidative hemolysis, possibly by affecting SH-groups. H2O2-producing compounds should be avoided in uremic patients.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Leber HW,Spiegelhalter R,Ulm A,Goubeaud G,Rawer P

doi

10.1111/j.1525-1594.1978.tb01626.x

subject

Has Abstract

pub_date

1978-11-01 00:00:00

pages

378-81

issue

4

eissn

0160-564X

issn

1525-1594

journal_volume

2

pub_type

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