Abstract:
:Using either hexazotized pararosaniline or new fuchsin as coupling agents, we investigated the ultrastructural localization of alpha-naphthyl acetate esterase (ANAE) activity in guinea pig bone marrow and peritoneal exudates, and in human peripheral blood cells. DFP-inhibitable ANAE activity was present on the cell surface of lymphocytes, monocytes, macrophages, neutrophils, eosinophils, basophils, megakaryocytes, platelets, and blasts. Demarcation lines in megakaryocytes and the perinuclear cisternae in normoblasts were also positive. In addition, lymphocytes, monocytes, and macrophages displayed ANAE activity associated with cytoplasmic-vesicle clusters (CVC). Reaction product was always present on the cytoplasmic surfaces of these vesicles and in the adjacent cytoplasm; vesicle interiors were invariably ANAE-negative. Small lymphocytes generally had a single large paranuclear ANAE-positive CVC, whereas mononuclear phagocytes had multiple discrete foci of similar appearing ANAE-positive CVC that sometime became confluent. ANAE activity was also found in the Gall bodies of human lymphocytes and in coated vesicles of macrophages. Cytoplasmic ANAE activity was increased in oil-induced guinea pig peritoneal macrophages. Both surface and cytoplasmic esterase activities had a neutral pH optimum. An identical distribution of reaction product was observed when alpha-naphthyl butyrate was employed as substrate. The function of these esterases, and their relation to known surface and cytoplasmic neutral proteases, awaits further investigation.
journal_name
Bloodjournal_title
Bloodauthors
Monahan RA,Dvorak HF,Dvorak AMsubject
Has Abstractpub_date
1981-12-01 00:00:00pages
1089-99issue
6eissn
0006-4971issn
1528-0020journal_volume
58pub_type
杂志文章相关文献
BLOOD文献大全abstract::Biosynthesis of leukotrienes (LTs) occurs in human myeloid cells and B lymphocytes. However, the function of leukotrienes in B lymphocytes is unclear. Here, we report that B-cell chronic lymphocytic leukemia (B-CLL) cells produce leukotriene B(4), and that specific leukotriene biosynthesis inhibitors counteracted CD40...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-07-2546
更新日期:2005-02-01 00:00:00
abstract::Recently, we described a new genetic disorder (the "hereditary hyperferritinemia-cataract syndrome") clinically characterized by the combination of elevated serum ferritin and congenital bilateral nuclear cataract, both cotransmitted as an autosomal dominant trait. In affected subjects, hyperferritinemia (ranging from...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::We show that lentiviral delivery of human gamma-globin gene under beta-globin regulatory control elements in hematopoietic stem cells (HSCs) results in sufficient postnatal fetal hemoglobin (HbF) expression to correct sickle cell anemia (SCA) in the Berkeley "humanized" sickle mouse. Upon de-escalating the amount of t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-201863
更新日期:2009-08-06 00:00:00
abstract::Combinations of proinflammatory and procoagulant reactions are the unifying principle for a variety of disorders affecting the cardiovascular system. The factor XII-driven contact system starts coagulation and inflammatory mechanisms via the intrinsic pathway of coagulation and the bradykinin-producing kallikrein-kini...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-04-569111
更新日期:2018-04-26 00:00:00
abstract::Primary cutaneous lymphomas (PCLs) are clonal T- or B-cell neoplasms, which originate in the skin. In recent years, mast cells were described as regulators of the tumor microenvironment in different human malignancies. Here, we investigated the role of mast cells in the tumor microenvironment of PCL. We found signific...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-03-415638
更新日期:2012-09-06 00:00:00
abstract::A novel Hodgkin's disease (HD) derived cell line, L1236, was established from the peripheral blood of a patient with advanced Hodgkin's disease. Analysis of immunoglobulin (Ig) gene rearrangements revealed a biallelic Ig heavy chain and a monoallelic Ig kappa light chain gene rearrangement, pointing to a B-lymphoid or...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-04-15 00:00:00
abstract::Recent advances in molecular biology have led to the identification of hematopoietic growth factors that support and influence the proliferation of hematopoietic progenitor cells in vitro and in vivo. Although these factors have been extensively studied, little is known of their role in the regulation of cell-cycling ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Trial ALL-BFM 90 was designed to improve outcome in patients with childhood acute lymphoblastic leukemia (ALL) by using a reduced treatment regimen. Patients were stratified into a standard-risk group (SRG), a medium-risk group (MRG), both defined by adequate early treatment response; and a high-risk group (HRG), defi...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2000-06-01 00:00:00
abstract::We prospectively evaluated 169 patients with a number of screening studies performed between 71 to 121 days after allogeneic marrow transplantation to detect the development of chronic graft-versus-host disease (GVHD). Group 1 patients (n = 78) were asymptomatic and had normal physical examinations at the time of scre...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::The expression of Lewis fucosyltransferase (FT) mRNA was examined in gastric mucosa from two Lewis-positive [Le(+)] and two Lewis-negative [Le(-)] individuals. Northern blot analysis demonstrated that levels of mRNA were similar in both Le(+) and Le(-) gastric mucosa. We isolated the protein-coding region of the Lewis...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::De novo erythroleukemia (EL) is a rare disease. Reported median survival are poor and vary from 4 to 14 months. The value of hematopoietic stem cell transplantation (HSCT) for EL is unknown. This EBMT registry study reports on the largest series of patients with EL treated with HSCT in first complete remission-103 aut...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood.V100.9.3135
更新日期:2002-11-01 00:00:00
abstract::Fibrin forms the cohesive network of hemostatic plugs and thrombi, and it also provides the temporary matrix for initial support of healing and revascularization. Because cell proliferation is needed for revascularization after vessel injury, we have characterized structural requirements of fibrin needed to support ce...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Hematopoiesis is tightly controlled by transcription regulatory networks, but how and when specific transcription factors control lineage commitment are still largely unknown. Within the hematopoietic stem cell (Lin(-)Sca-1(+)c-Kit(+)) compartment these lineage-specific transcription factors are expressed at low level...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-03-275404
更新日期:2010-11-18 00:00:00
abstract::Dysregulation of polycomb repressive complex 2 (PRC2) promotes oncogenesis partly through its enzymatic function for inducing trimethylation of histone H3 lysine 27 (H3K27me3). However, it remains to be determined how PRC2 activity is regulated in normal and diseased settings. We here report a PRC2-associated cofactor...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019000578
更新日期:2019-10-03 00:00:00
abstract::Expression of the arterial marker molecule ephrinB2 in endothelial cells is a prerequisite for adequate remodeling processes of the developing or angiogenic vasculature. Although its role in these processes has been extensively studied, the impact of ephrinB2 on the remodeling of adult arteries is largely unknown. To ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-12-128835
更新日期:2008-07-01 00:00:00
abstract::The interaction between platelet receptor glycoprotein Ibα and the A1 domain of von Willebrand factor (VWF) mediates tethering/translocation of platelets to sites of vascular injury. Unexpectedly, we observed platelets translocating over A1A2A3 domains protein slower than on A1 domain at high shear stress. This observ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-530428
更新日期:2014-04-24 00:00:00
abstract::The regulation of CCR6 (chemokine receptor 6) expression during B-cell ontogeny and antigen-driven B-cell differentiation was analyzed. None of the CD34(+)Lin(-) hematopoietic stem cell progenitors or the CD34(+)CD19(+) (pro-B) or the CD19(+)CD10(+) (pre-B/immature B cells) B-cell progenitors expressed CCR6. CCR6 is a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Mig, the monokine induced by interferon-gamma, is a CXC chemokine active as a chemoattractant for activated T cells. Mig is related functionally to interferon-inducible protein 10 (IP-10), with which it shares a receptor, CXCR3. Previously, IP-10 was found to have antitumor activity in vivo. In the present study, muri...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-04-15 00:00:00
abstract::Studies with the granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin-3 (IL-3) fusion protein, PIXY321, demonstrated enhanced biological activity of this molecule in comparison with GM-CSF or IL-3 alone or in combination. Experiments were performed to study the mechanisms resulting in PIXY321-induced ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::All-trans retinoic acid (tRA) and arsenic trioxide (As(2)O(3)) induce non-cross-resistant complete clinical remission in patients with acute promyelocytic leukemia with t(15;17) translocation and target PML-RARalpha, the leukemogenic protein, by different pathways suggesting a possible therapeutic synergism. To evalua...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.1.264
更新日期:2001-01-01 00:00:00
abstract::The Hermes receptor CD44 is a multifunctional adhesion molecule that plays an essential role in the homing and invasion of neoplastic stem cells in various myeloid malignancies. Although mast cells (MCs) reportedly express CD44, little is known about the regulation and function of this receptor in neoplastic cells in ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-02-833582
更新日期:2018-11-01 00:00:00
abstract::The treatment of multiple myeloma is considered a continuously evolving paradigm as a result of the growing availability of new and highly effective drugs, including first- and second-generation proteasome inhibitors, immunomodulatory agents, and monoclonal antibodies. Clinical trials advocate long-term rather than sh...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-01-725705
更新日期:2017-04-27 00:00:00
abstract::We analyzed the incidence of posttransplant chronic myelogenous leukemia (CML) relapse in 283 consecutive related-donor (n = 177) and unrelated-donor (n = 106) allogeneic transplant recipients. Twenty-two of 165 related-donor recipients with stable or advanced disease at the time of transplant had hematologic relapse ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-07-15 00:00:00
abstract::Acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) are associated with disease-initiating stem cells that are not eliminated by conventional therapies. Novel therapeutic targets against preleukemic stem cells need to be identified for potentially curative strategies. We conducted parallel transcriptional...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-01-621631
更新日期:2015-05-14 00:00:00
abstract::A mechanism to control development of mast cells was investigated in mice. Although mast cells in the skin of normal C57BL/6 mice were still of host type 290 days after irradiation and injection of bone marrow cells from beige (Chediak-Higashi syndrome, C57BL/6 bgJ/bgJ) mice, donor-type mast cells with giant granules ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::In Wiskott-Aldrich syndrome (WAS), immunodeficiency and autoimmunity often comanifest, yet how WAS mutations misregulate chromatin-signaling in Thelper (TH) cells favoring development of auto-inflammation over protective immunity is unclear. Previously, we identified an essential promoter-specific, coactivator role of...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-05-646182
更新日期:2015-10-01 00:00:00
abstract::Maintaining a steady pool of self-renewing hematopoietic stem cells (HSCs) is critical for sustained production of multiple blood lineages. Many transcription factors and molecules involved in chromatin and epigenetic modifications have been found to be critical for HSC self-renewal and differentiation; however, their...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-306563
更新日期:2011-02-17 00:00:00
abstract::Dendritic cells (DC) are highly efficient antigen-presenting cells (APC) that have an essential function in the development of immune responses against microbial pathogens and tumors. Although during the past few years our understanding of DC biology has remarkably increased, a precise characterization of the differen...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-01-15 00:00:00
abstract::The interaction of von Willebrand factor (vWF) with the alpha v beta 3 integrin of human umbilical vein endothelial cells is dependent on the RGD sequence present at residues 1744-1746 of the mature vWF subunit. We compared vWF and its two dimeric fragments, SpIII (residues 1-1365) and SpII (residues 1366-2050), as ad...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-12-15 00:00:00
abstract::Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages and a robust ex vivo P vi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-08-596015
更新日期:2015-02-19 00:00:00