Abstract:
:The rate of depolymerization of microtubules upon lowering the temperature was found to depend on the amount of time elapsed since the beginning of the assembly process. In the first minutes following self-assembly at 37 degrees C, microtubules are more cold sensitive and depolymerize faster than later at the steady state. In the meanwhile, no change occurred in the average length nor in the shape of the distribution of microtubules. On the other hand, the evolution with time of the apparent dissociation rate constant of tubulin from microtubules was in good correlation with the GTP content of microtubules following assembly, showing that GTP hydrolysis modifies the tubulin-tubulin interactions. Microtubule-bound GTP was not exchangeable for GDP, but steady-state GTP hydrolysis was inhibited by GDP. This result indicates that GDP and GTP exhibit different affinities for tubulin in the body and at the ends of microtubules. It is proposed that GTP-tubulin dissociates faster from microtubules than GDP-tubulin. In other words GTP hydrolysis contributes to the stabilization of microtubules.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bonne D,Pantaloni Ddoi
10.1021/bi00534a039subject
Has Abstractpub_date
1982-03-02 00:00:00pages
1075-81issue
5eissn
0006-2960issn
1520-4995journal_volume
21pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Escherichia coli were grown on 14.3% uniformly 13C-labeled glucose as the sole carbon source and challenged anaerobically with 90% 13C-labeled formaldehyde. The major multiply labeled metabolites were identified by 13C NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-prop...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a050
更新日期:1985-07-16 00:00:00
abstract::In recent years, there has been dramatic growth in the study of RNA. RNA has gone from being known as an intermediate in the central dogma of molecular biology to a molecule with a large diversity of structure and function that is involved in all aspects of biology. As new functions are rapidly discovered, it has beco...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01141
更新日期:2018-08-07 00:00:00
abstract::Chloramphenicol acetyltransferase (CAT) reversibly transfers an acetyl group between CoA and the 3-hydroxyl of either chloramphenicol (Cm) or 1-acetylchloramphenicol (1AcCm). The products of the forward reactions, 3-acetylchloramphenicol (3-AcCm) and 1,3-diacetylchloramphenicol (1,3Ac2-Cm), are the substrates for the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00199a003
更新日期:1994-08-23 00:00:00
abstract::A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960845g
更新日期:1996-09-10 00:00:00
abstract::The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962219p
更新日期:1997-03-18 00:00:00
abstract::The effects of site-directed mutagenesis were used to explore the role of residues in M4 on the apparent Ki of a selective, K+-competitive inhibitor of the gastric H+,K+ ATPase, SCH28080. A double transfection expression system is described, utilizing HEK293 cells and separate plasmids encoding the alpha and beta subu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991837d
更新日期:2000-03-21 00:00:00
abstract::The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00344a013
更新日期:1985-11-05 00:00:00
abstract::Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701950j
更新日期:2008-02-26 00:00:00
abstract::The structures of four isomorphous crystals of ternary complexes of chicken heart citrate synthase with D- or L-malate and acetyl coenzyme A or carboxymethyl coenzyme A have been determined by X-ray crystallography and fully refined at 1.9-A resolution. The structures show that both L-malate and D-malate bind in a ver...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00238a028
更新日期:1991-06-18 00:00:00
abstract::The fluorophore 4-heptadecyl-7-hydroxycoumarin was used as a probe to study the properties of phospholipid bilayers at the lipid-water interface. To this end, the steady-state fluorescence anisotropy, the differential polarized phase fluorometry, and the emission lifetime of the fluorophore were measured in isotropic ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00324a005
更新日期:1985-01-29 00:00:00
abstract::NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9920230
更新日期:2000-02-08 00:00:00
abstract::The second-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cyclic voltammetry utilizing a gold electrode modified with beta-mercaptopropionate. When cyclic voltammetry is performed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9529723
更新日期:1996-09-24 00:00:00
abstract::The mechanism by which some hydrophobic molecules such as steroids and free fatty acids (FFA) act as noncompetitive inhibitors of the nicotinic acetylcholine receptor (AChR) is still not known. In the present work, we employ Förster resonance energy transfer (FRET) between the intrinsic fluorescence of membrane-bound ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061388z
更新日期:2007-03-20 00:00:00
abstract::A combined N- and C-terminal truncation variant of human apolipoprotein A-I (apoA-I) was designed, expressed in Escherichia coli, isolated, and characterized. Hydrodynamic experiments yielded a weight average molecular weight of 34000, indicating apoA-I-(44-186) exists in solution predominantly as a dimer. An axial ra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0477135
更新日期:2005-03-22 00:00:00
abstract::Two nucleoside diphosphate analogs, 3'-C-methyl-ADP and 3'-C-methyl-UDP, have been tested as substrate and/or allosteric effectors using the adenosylcobalamin-dependent ribonucleoside diphosphate reductase of Corynebacterium nephridii. Neither analog was a substrate for the reductase. However, they did function as all...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a035
更新日期:1992-11-17 00:00:00
abstract::Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00428a038
更新日期:1989-01-24 00:00:00
abstract::The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8013032
更新日期:2009-01-13 00:00:00
abstract::A well-defined region of pancreatic and other secreted phospholipase A2 (PLA2), which we call the i-face, makes a molecular contact with the interface to facilitate and control the events and processivity of the interfacial catalytic turnover cycles. The structural features of the i-face and its allosteric relationshi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9925470
更新日期:2000-05-16 00:00:00
abstract::The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00523a010
更新日期:1981-09-29 00:00:00
abstract::Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400197u
更新日期:2013-05-07 00:00:00
abstract::The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980139a
更新日期:1998-06-09 00:00:00
abstract::To gain insight into the mechanism by which Arg-163 influences oligomerization of alphaA-crystallin, we prepared a series of truncated alphaA-crystallins with or without mutation of the Arg-163 residue. Expression of the proteins was achieved in Escherichia coli BL21 (DE3) pLysS cells, and alphaA-crystallin was purifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060705z
更新日期:2006-12-26 00:00:00
abstract::Peripherin/rds is a 39 kDa integral membrane glycoprotein essential for normal photoreceptor cell development in vertebrates. It has been implicated in several human retinal degenerative diseases including retinitis pigmentosa and macular degeneration and is thought to play a structural role at photoreceptor outer seg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00043a028
更新日期:1995-10-31 00:00:00
abstract::The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9626158
更新日期:1997-04-01 00:00:00
abstract::Oligonucleotides capable of sequence-specific triple helix formation have been proposed as DNA binding ligands useful for modulation of gene expression and for directed genome modification. However, the effectiveness of such triplex-forming oligonucleotides (TFOs) depends on their ability to bind to their target sites...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982290q
更新日期:1999-02-09 00:00:00
abstract::A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9914964
更新日期:2000-01-11 00:00:00
abstract::Neisseria meningitidis α-1,4-galactosyltransferase C (LgtC) is responsible for the transfer of α-galactose from donor UDP-galactose to the lipooligosaccharide terminal acceptor lactose. Crystal structures of its substrate analogue complexes have provided key insights into the galactosyl transfer mechanism, including a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301317d
更新日期:2013-01-15 00:00:00
abstract::We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a020
更新日期:1993-08-24 00:00:00
abstract::The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00963
更新日期:2016-12-06 00:00:00
abstract::Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00281a026
更新日期:1983-06-07 00:00:00