Abstract:
:We have prepared antibody/3H-dsDNA immune complexes and have used three independent radioimmunoassays to quantitate their interaction with complement; the solution phase C1q assay, the Raji cell assay, and a complement-based red blood cell adherence assay (RBC-CF). Our results indicate that although there is reasonable qualitative agreement between the Raji cell assay and the RBC-CF assay, there are some differences in the quantitative range of sensitivities of the two assays. On the other hand, we find that most of the complement-fixing antibody/3H-dsDNA complexes are not detected in the solution phase C1q assay. The results suggest this is because the absolute concentrations of the immune complexes were too low to achieve significant precipitation under the standard conditions used in the C1q assay. The implication of these findings with respect to the potential detection and analysis of antibody/dsDNA immune complexes is discussed.
journal_name
Rheumatol Intjournal_title
Rheumatology internationalauthors
Taylor RP,Andrews BS,Morley KW,Conlon Tdoi
10.1007/BF00541220subject
Has Abstractpub_date
1981-01-01 00:00:00pages
29-34issue
1eissn
0172-8172issn
1437-160Xjournal_volume
1pub_type
杂志文章abstract::Nitric oxide (NO) is a free radical that plays important roles in many physiological and pathological processes. Evidence suggests that NO participates in the pathogenesis of inflammatory reactions in many autoimmune and inflammatory diseases such as rheumatoid arthritis (RA). The purpose of this study was to evaluate...
journal_title:Rheumatology international
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s002960100105
更新日期:2001-05-01 00:00:00
abstract::Smoking produces substances that activate proinflammatory, prothrombotic and vasoconstrictive mediators via posttranslational carbamylation of proteins. As a new consequence of carbamylation, induction of anti-carbamylated autoantibodies were observed in rheumatoid arthritis (RA) patients, sometimes prior to onset of ...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-016-3472-9
更新日期:2016-06-01 00:00:00
abstract::Plasma fibronectin (FN) was measured in 17 patients with essential mixed cryoglobulinaemia (EMC) and in 17 normal subjects by single radial immunodiffusion (RID) and enzyme-linked immunosorbent assay (ELISA). In 9 patients the presence of FN in the cryoprecipitates was also assessed by immunoblotting. In the EMC group...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/BF00541379
更新日期:1987-01-01 00:00:00
abstract::Both self-reported and physical performance tests are used as outcome measures in knee osteoarthritis (OA). The aim of this study is to investigate the relationship between Knee Injury and Osteoarthritis Outcome Score (KOOS) and Timed Up and Go (TUG) test in the patients with symptomatic knee OA. Eighty-nine patients ...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-012-2512-3
更新日期:2013-10-01 00:00:00
abstract::Wegener granulomatosis (WG) is a necrotizing granulomatous vasculitis that predominantly affects airways and kidneys. But central nervous system involvement (7-11%) is an uncommon. Massive ICH may occur in the course of WG, and this serious condition is related with high risk of mortality. Therefore, the new treatment...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-011-1954-3
更新日期:2012-06-01 00:00:00
abstract::We sought to determine the prevalence of additional connective tissue diseases (CTDs) in patients with idiopathic inflammatory myopathies (IIM), and to study the muscle biopsy patterns in various clinico-serologic subsets of myositis. We undertook a retrospective cohort study of 648 patients with a histological diagno...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-019-04411-8
更新日期:2019-10-01 00:00:00
abstract::Seven females and one male with almost identical sclerotic and hyperostotic changes of the manubrium sterni are reported. The clinical course was prolonged and characterized by intermittent periods of exacerbation followed by improvement. Malignancies, bacterial inflammatory processes, and Paget disease, which were fi...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/BF00541284
更新日期:1986-01-01 00:00:00
abstract::Protein synthesis in all cells is readily interrupted by environmental stresses such as hyperthermia. However, stressed cells also have mechanisms which, even under conditions where normal protein synthesis is completely inhibited, allow them to synthesise a group of highly conserved proteins--the so-called heat shock...
journal_title:Rheumatology international
pub_type: 杂志文章,评审
doi:10.1007/BF00541323
更新日期:1990-01-01 00:00:00
abstract::Systematic reviews and meta-analyses of randomized trials have long been important synthesis tools for guiding evidence-based medicine. More recently, network meta-analyses, an extension of traditional meta-analyses enabling the comparison of multiple interventions, use new statistical methods to incorporate clinical ...
journal_title:Rheumatology international
pub_type: 杂志文章,评审
doi:10.1007/s00296-014-2994-2
更新日期:2014-11-01 00:00:00
abstract::PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotti...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-009-0870-2
更新日期:2009-10-01 00:00:00
abstract:UNLABELLED:The objective of this study is to provide a critical overview of available instruments to assess impairments in patients with rheumatic disorders, and to recommend reliable and valid instruments for use in allied health care and rehabilitation medicine. A computer-aided literature search (1982-2004) in sever...
journal_title:Rheumatology international
pub_type: 杂志文章,评审
doi:10.1007/s00296-005-0603-0
更新日期:2005-09-01 00:00:00
abstract::In a randomized multicenter, double-blind, double-dummy, parallel group study a comparison of the efficacy and safety of 1 microg alfacalcidol to 880 IU vitamin D plus calcium carbonate (1 g calcium) once daily per os was performed on 148 postmenopausal osteoporotic Caucasian patients with normal vitamin D serum level...
journal_title:Rheumatology international
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00296-005-0073-4
更新日期:2006-03-01 00:00:00
abstract::As has been shown by a number of working groups, primary fibromyalgia syndrome does not represent a single clinical entity. It is possible to distinguish between a subgroup with high pain sensitivity and no associated psychiatric condition, a second and a third subgroup characterized by depression associated with fibr...
journal_title:Rheumatology international
pub_type: 杂志文章,评审
doi:10.1007/s00296-007-0403-9
更新日期:2007-09-01 00:00:00
abstract::Alkoptunuria is an inherited autosomal recessive metabolic disorder which is caused by the lack of homogentisic acid-oxidase enzyme. It is associated with various systemic abnormalities and related to the deposition of homogentisic acid pigment in connective tissues. These pigmentary changes are termed "ochronosis". W...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-004-0538-x
更新日期:2005-08-01 00:00:00
abstract::Systemic lupus erythematosus is an autoimmune disease with protean manifestation. Arthritis is one of the most common manifestations seen in SLE. Anti-CCP Ab is a recently described autoantibody that has been claimed as a most sensitive and specific marker for the diagnosis of RA. Study was performed to see whether an...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-010-1374-9
更新日期:2011-06-01 00:00:00
abstract::Hydroxyapatite crystals are often deposited in the vicinity of joints, where they can cause a clinical periarthritis. Clodronate is a first-generation bisphosphonate that has the ability to reduce ectopic calcifications. Two women were affected by disabling calcific periarthritis of the shoulders lasting for years and...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-010-1575-2
更新日期:2011-03-01 00:00:00
abstract::The exact elucidation of skeletal and cartilagineous involvement in neonatal-onset multisystem inflammatory disease (NOMID) is still poorly known, and there are few data providing the long-term response to treatment with the available interleukin-1 inhibitors. We present here a 13-year-old boy with NOMID treated with ...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-010-1787-5
更新日期:2011-12-01 00:00:00
abstract::Synovial fluids of patients suffering from rheumatoid arthritis contain elevated levels of granulocyte (PMN) elastase in complex with alpha 1-proteinase inhibitor (alpha 1-PI), whereas free-elastase activity is usually not detectable. This absence of free enzymatic activity in joint effusions has cast some doubt on th...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/BF00270466
更新日期:1987-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic, progressive, debilitating disease that demands continuous therapy with multiple medications. Noncompliance with disease-modifying drugs may cause disease flares, preventable functional impairment, unnecessary treatment changes, and loss of health care resources. The aim of the c...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-011-2162-x
更新日期:2012-10-01 00:00:00
abstract::The Juvenile Arthritis Multidimensional Assessment Report (JAMAR) is a new parent/patient reported outcome measure that enables a thorough assessment of the disease status in children with juvenile idiopathic arthritis (JIA). We report the results of the cross-cultural adaptation and validation of the parent and patie...
journal_title:Rheumatology international
pub_type: 杂志文章,多中心研究
doi:10.1007/s00296-018-3980-x
更新日期:2018-04-01 00:00:00
abstract::Tuberculosis (TB) is an important preventable infection in patients with rheumatoid arthritis (RA). To determine the risk of TB in patients with RA by comparing with those with non-specific back pain (NSBP), and to identify the risk factors in the RA group. Medical data were retrieved from a centralized electronic dat...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-020-04583-8
更新日期:2020-06-01 00:00:00
abstract::The study objective was to examine natural variation of the patient-reported outcome measures fatigue, pain, patient global assessment (PaGl) and the Bath Ankylosing Spondylitis Functional Index (BASFI) in patients with stable axial spondyloarthropathy (ax-SpA) defined on the basis of the Bath Spondylitis Ankylosing D...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-017-3920-1
更新日期:2018-03-01 00:00:00
abstract::There are numerous causes of pulmonary cavitary lesions as infection (bacterial, parasitic and invasive fungal), Wegener granulomatosis (WG) and other vasculitis, sarcoidosis, malignancy, septic thromboembolism, airways disease (cystic bronchiectasis and bullae), pneumatoceles and traumatic parenchymal laceration. Her...
journal_title:Rheumatology international
pub_type: 信件
doi:10.1007/s00296-012-2424-2
更新日期:2013-08-01 00:00:00
abstract::Osteoporosis has become an increasingly recognized complication among patients with chronic liver disease (CLD). The aim of the present study was to assess the prevalence and risk factors of osteoporosis in patients with CLD (primary biliary cirrhosis and chronic viral hepatitis B or C patients) in comparison with a g...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-009-1071-8
更新日期:2010-05-01 00:00:00
abstract::The aim of this study is to observe the effect of different treatment time of millimeter wave (MMW) on chondrocyte apoptosis, caspase-3, caspase-8, and matrix metalloproteinase-13 (MMP-13) in rabbit knee osteoarthritis induced by anterior cruciate ligament transection (ACLT). Thirty-two New Zealand White rabbits were ...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-011-2080-y
更新日期:2012-09-01 00:00:00
abstract::Various surgical procedures including percutaneous and open release and arthroscopic procedures have been described to treat recalcitrant tennis elbow. We present the outcome of Boyd-McLeod surgical procedure for tennis elbow resistant to non-operative treatment in twenty-seven patients (twenty-nine limbs). Boyd McLeo...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-010-1450-1
更新日期:2011-08-01 00:00:00
abstract::Microvascular lesions are a predominant feature in systemic sclerosis (SSc) and seem to play a central pathogenic role. The presence of nailfold capillary abnormalities is useful in diagnosing SSc. Capillaroscopy, however, usually requires special equipment and may be time consuming. Dermatoscope has been presented as...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-010-1532-0
更新日期:2011-12-01 00:00:00
abstract::We describe a typical case of a patient with remitting seronegative symmetrical synovitis and pitting edema (RS(3)PE) syndrome. He underwent a successful clinical course monitored by serologic variables and radiographic methods. Serum levels of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), matrix me...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-009-1142-x
更新日期:2010-11-01 00:00:00
abstract::Leflunomide is a new immunosuppressive medicine that has been effectively used in the therapy of rheumatoid arthritis and subsequently used with success in animal models and patients with systemic lupus erythematosus (SLE). However, its use has also been associated with significant and serious adverse reactions involv...
journal_title:Rheumatology international
pub_type: 杂志文章,评审
doi:10.1007/s00296-012-2508-z
更新日期:2013-02-01 00:00:00
abstract::The current clinical and laboratory parameters of Takayasu arteritis (TA) are insufficient for proper assessment of disease activity. The aims of this study were to investigate the markers of endothelial injury and repair, including circulating endothelial cell (CEC), circulating endothelial progenitor cell (CEPC) and...
journal_title:Rheumatology international
pub_type: 杂志文章
doi:10.1007/s00296-013-2937-3
更新日期:2014-08-01 00:00:00