Quantitative studies of the interaction of 3H-dsDNA/anti-DNA immune complexes with complement: comparison and evaluation of the Raji cell, the solution phase C1Q, and the red blood cell linked complement fixation radioimmunoassays.

Abstract:

:We have prepared antibody/3H-dsDNA immune complexes and have used three independent radioimmunoassays to quantitate their interaction with complement; the solution phase C1q assay, the Raji cell assay, and a complement-based red blood cell adherence assay (RBC-CF). Our results indicate that although there is reasonable qualitative agreement between the Raji cell assay and the RBC-CF assay, there are some differences in the quantitative range of sensitivities of the two assays. On the other hand, we find that most of the complement-fixing antibody/3H-dsDNA complexes are not detected in the solution phase C1q assay. The results suggest this is because the absolute concentrations of the immune complexes were too low to achieve significant precipitation under the standard conditions used in the C1q assay. The implication of these findings with respect to the potential detection and analysis of antibody/dsDNA immune complexes is discussed.

journal_name

Rheumatol Int

authors

Taylor RP,Andrews BS,Morley KW,Conlon T

doi

10.1007/BF00541220

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

29-34

issue

1

eissn

0172-8172

issn

1437-160X

journal_volume

1

pub_type

杂志文章
  • Elevated levels of nitrate in rheumatoid arthritis.

    abstract::Nitric oxide (NO) is a free radical that plays important roles in many physiological and pathological processes. Evidence suggests that NO participates in the pathogenesis of inflammatory reactions in many autoimmune and inflammatory diseases such as rheumatoid arthritis (RA). The purpose of this study was to evaluate...

    journal_title:Rheumatology international

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s002960100105

    authors: Onur O,Akinci AS,Akbiyik F,Unsal I

    更新日期:2001-05-01 00:00:00

  • Carbamylated vimentin represents a relevant autoantigen in Latin American (Cuban) rheumatoid arthritis patients.

    abstract::Smoking produces substances that activate proinflammatory, prothrombotic and vasoconstrictive mediators via posttranslational carbamylation of proteins. As a new consequence of carbamylation, induction of anti-carbamylated autoantibodies were observed in rheumatoid arthritis (RA) patients, sometimes prior to onset of ...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-016-3472-9

    authors: Martínez G,Gómez JA,Bang H,Martínez-Gamboa L,Roggenbuck D,Burmester GR,Torres B,Prada D,Feist E

    更新日期:2016-06-01 00:00:00

  • Plasma fibronectin and microvascular damage in essential mixed cryoglobulinaemia.

    abstract::Plasma fibronectin (FN) was measured in 17 patients with essential mixed cryoglobulinaemia (EMC) and in 17 normal subjects by single radial immunodiffusion (RID) and enzyme-linked immunosorbent assay (ELISA). In 9 patients the presence of FN in the cryoprecipitates was also assessed by immunoblotting. In the EMC group...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/BF00541379

    authors: Toschi V,Renoldi P,Motta A,Cimminiello C,Arpaia G,Fiorini GF

    更新日期:1987-01-01 00:00:00

  • The relationship between Knee Injury and Osteoarthritis Outcome Score (KOOS) and Timed Up and Go test in patients with symptomatic knee osteoarthritis.

    abstract::Both self-reported and physical performance tests are used as outcome measures in knee osteoarthritis (OA). The aim of this study is to investigate the relationship between Knee Injury and Osteoarthritis Outcome Score (KOOS) and Timed Up and Go (TUG) test in the patients with symptomatic knee OA. Eighty-nine patients ...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-012-2512-3

    authors: Sabirli F,Paker N,Bugdayci D

    更新日期:2013-10-01 00:00:00

  • Massive intracerebral hemorrhage associated with Wegener granulomatosis.

    abstract::Wegener granulomatosis (WG) is a necrotizing granulomatous vasculitis that predominantly affects airways and kidneys. But central nervous system involvement (7-11%) is an uncommon. Massive ICH may occur in the course of WG, and this serious condition is related with high risk of mortality. Therefore, the new treatment...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-011-1954-3

    authors: Ceri M,Ortabozkoyun L,Unverdi S,Kirac M,Duranay M

    更新日期:2012-06-01 00:00:00

  • Prevalence of other connective tissue diseases in idiopathic inflammatory myopathies.

    abstract::We sought to determine the prevalence of additional connective tissue diseases (CTDs) in patients with idiopathic inflammatory myopathies (IIM), and to study the muscle biopsy patterns in various clinico-serologic subsets of myositis. We undertook a retrospective cohort study of 648 patients with a histological diagno...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-019-04411-8

    authors: Maundrell A,Proudman S,Limaye V

    更新日期:2019-10-01 00:00:00

  • Sclerosis and hyperostosis of the manubrium sterni.

    abstract::Seven females and one male with almost identical sclerotic and hyperostotic changes of the manubrium sterni are reported. The clinical course was prolonged and characterized by intermittent periods of exacerbation followed by improvement. Malignancies, bacterial inflammatory processes, and Paget disease, which were fi...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/BF00541284

    authors: Jurik AG,Møller BN,Jensen MK,Jensen JT,Graudal H

    更新日期:1986-01-01 00:00:00

  • Stress (heat shock) proteins and rheumatic disease. New advance or just another band wagon?

    abstract::Protein synthesis in all cells is readily interrupted by environmental stresses such as hyperthermia. However, stressed cells also have mechanisms which, even under conditions where normal protein synthesis is completely inhibited, allow them to synthesise a group of highly conserved proteins--the so-called heat shock...

    journal_title:Rheumatology international

    pub_type: 杂志文章,评审

    doi:10.1007/BF00541323

    authors: Hurst NP

    更新日期:1990-01-01 00:00:00

  • Network meta-analysis for comparing treatment effects of multiple interventions: an introduction.

    abstract::Systematic reviews and meta-analyses of randomized trials have long been important synthesis tools for guiding evidence-based medicine. More recently, network meta-analyses, an extension of traditional meta-analyses enabling the comparison of multiple interventions, use new statistical methods to incorporate clinical ...

    journal_title:Rheumatology international

    pub_type: 杂志文章,评审

    doi:10.1007/s00296-014-2994-2

    authors: Catalá-López F,Tobías A,Cameron C,Moher D,Hutton B

    更新日期:2014-11-01 00:00:00

  • The expression of PADI4 in synovium of rheumatoid arthritis.

    abstract::PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotti...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-009-0870-2

    authors: Chang X,Zhao Y,Sun S,Zhang Y,Zhu Y

    更新日期:2009-10-01 00:00:00

  • Impairment measures in rheumatic disorders for rehabilitation medicine and allied health care: a systematic review.

    abstract:UNLABELLED:The objective of this study is to provide a critical overview of available instruments to assess impairments in patients with rheumatic disorders, and to recommend reliable and valid instruments for use in allied health care and rehabilitation medicine. A computer-aided literature search (1982-2004) in sever...

    journal_title:Rheumatology international

    pub_type: 杂志文章,评审

    doi:10.1007/s00296-005-0603-0

    authors: Swinkels RA,Bouter LM,Oostendorp RA,van den Ende CH

    更新日期:2005-09-01 00:00:00

  • Superiority of alfacalcidol compared to vitamin D plus calcium in lumbar bone mineral density in postmenopausal osteoporosis.

    abstract::In a randomized multicenter, double-blind, double-dummy, parallel group study a comparison of the efficacy and safety of 1 microg alfacalcidol to 880 IU vitamin D plus calcium carbonate (1 g calcium) once daily per os was performed on 148 postmenopausal osteoporotic Caucasian patients with normal vitamin D serum level...

    journal_title:Rheumatology international

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1007/s00296-005-0073-4

    authors: Nuti R,Bianchi G,Brandi ML,Caudarella R,D'Erasmo E,Fiore C,Isaia GC,Luisetto G,Muratore M,Oriente P,Ortolani S

    更新日期:2006-03-01 00:00:00

  • The classification of fibromyalgia syndrome.

    abstract::As has been shown by a number of working groups, primary fibromyalgia syndrome does not represent a single clinical entity. It is possible to distinguish between a subgroup with high pain sensitivity and no associated psychiatric condition, a second and a third subgroup characterized by depression associated with fibr...

    journal_title:Rheumatology international

    pub_type: 杂志文章,评审

    doi:10.1007/s00296-007-0403-9

    authors: Müller W,Schneider EM,Stratz T

    更新日期:2007-09-01 00:00:00

  • Ochronotic arthritis: case reports and review of the literature.

    abstract::Alkoptunuria is an inherited autosomal recessive metabolic disorder which is caused by the lack of homogentisic acid-oxidase enzyme. It is associated with various systemic abnormalities and related to the deposition of homogentisic acid pigment in connective tissues. These pigmentary changes are termed "ochronosis". W...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-004-0538-x

    authors: Cetinus E,Cever I,Kural C,Erturk H,Akyildiz M

    更新日期:2005-08-01 00:00:00

  • Anticyclic citrullinated peptide autoantibodies in systemic lupus erythematosus.

    abstract::Systemic lupus erythematosus is an autoimmune disease with protean manifestation. Arthritis is one of the most common manifestations seen in SLE. Anti-CCP Ab is a recently described autoantibody that has been claimed as a most sensitive and specific marker for the diagnosis of RA. Study was performed to see whether an...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-010-1374-9

    authors: Singh U,Singh S,Singh NK,Verma PK,Singh S

    更新日期:2011-06-01 00:00:00

  • Can clodronate be effective in the treatment of disabling hydroxyapatite crystal-deposition disease? A report of two cases.

    abstract::Hydroxyapatite crystals are often deposited in the vicinity of joints, where they can cause a clinical periarthritis. Clodronate is a first-generation bisphosphonate that has the ability to reduce ectopic calcifications. Two women were affected by disabling calcific periarthritis of the shoulders lasting for years and...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-010-1575-2

    authors: Saviola G,Abdi-Ali L,Baiardi P,Benucci M

    更新日期:2011-03-01 00:00:00

  • Long-term response after 6-year treatment with anakinra and onset of focal bone erosion in neonatal-onset multisystem inflammatory disease (NOMID/CINCA).

    abstract::The exact elucidation of skeletal and cartilagineous involvement in neonatal-onset multisystem inflammatory disease (NOMID) is still poorly known, and there are few data providing the long-term response to treatment with the available interleukin-1 inhibitors. We present here a 13-year-old boy with NOMID treated with ...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-010-1787-5

    authors: Rigante D,Leone A,Marrocco R,Laino ME,Stabile A

    更新日期:2011-12-01 00:00:00

  • Interference of cartilage surface with interaction of granulocyte elastase with alpha 1-proteinase inhibitor. An in vitro model of enzyme inhibition in the joint space.

    abstract::Synovial fluids of patients suffering from rheumatoid arthritis contain elevated levels of granulocyte (PMN) elastase in complex with alpha 1-proteinase inhibitor (alpha 1-PI), whereas free-elastase activity is usually not detectable. This absence of free enzymatic activity in joint effusions has cast some doubt on th...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/BF00270466

    authors: Burkhardt H,Kasten M,Rauls S,Rehkopf E

    更新日期:1987-01-01 00:00:00

  • Compliance with treatment of rheumatoid arthritis.

    abstract::Rheumatoid arthritis (RA) is a chronic, progressive, debilitating disease that demands continuous therapy with multiple medications. Noncompliance with disease-modifying drugs may cause disease flares, preventable functional impairment, unnecessary treatment changes, and loss of health care resources. The aim of the c...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-011-2162-x

    authors: Müller R,Kallikorm R,Põlluste K,Lember M

    更新日期:2012-10-01 00:00:00

  • The Afrikaans version of the Juvenile Arthritis Multidimensional Assessment Report (JAMAR).

    abstract::The Juvenile Arthritis Multidimensional Assessment Report (JAMAR) is a new parent/patient reported outcome measure that enables a thorough assessment of the disease status in children with juvenile idiopathic arthritis (JIA). We report the results of the cross-cultural adaptation and validation of the parent and patie...

    journal_title:Rheumatology international

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s00296-018-3980-x

    authors: Scott C,Okong'o L,Brice N,Murless S,Slamang W,Fadlelmola A,Consolaro A,Bovis F,Ruperto N,Paediatric Rheumatology International Trials Organisation (PRINTO).

    更新日期:2018-04-01 00:00:00

  • A retrospective study on the risk of tuberculosis in patients with rheumatoid arthritis.

    abstract::Tuberculosis (TB) is an important preventable infection in patients with rheumatoid arthritis (RA). To determine the risk of TB in patients with RA by comparing with those with non-specific back pain (NSBP), and to identify the risk factors in the RA group. Medical data were retrieved from a centralized electronic dat...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-020-04583-8

    authors: Chung TT,Ko HJ,Lau CS,Chung HY

    更新日期:2020-06-01 00:00:00

  • Stability of fatigue, pain, patient global assessment and the Bath Ankylosing Spondylitis Functional Index (BASFI) in spondyloarthropathy patients with stable disease according to the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI).

    abstract::The study objective was to examine natural variation of the patient-reported outcome measures fatigue, pain, patient global assessment (PaGl) and the Bath Ankylosing Spondylitis Functional Index (BASFI) in patients with stable axial spondyloarthropathy (ax-SpA) defined on the basis of the Bath Spondylitis Ankylosing D...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-017-3920-1

    authors: Madsen OR

    更新日期:2018-03-01 00:00:00

  • A confusing case: pulmonary lesions including cavities, isolated left heart endocarditis and inferior vena cava thrombosis in a patient with perforated diverticulitis.

    abstract::There are numerous causes of pulmonary cavitary lesions as infection (bacterial, parasitic and invasive fungal), Wegener granulomatosis (WG) and other vasculitis, sarcoidosis, malignancy, septic thromboembolism, airways disease (cystic bronchiectasis and bullae), pneumatoceles and traumatic parenchymal laceration. Her...

    journal_title:Rheumatology international

    pub_type: 信件

    doi:10.1007/s00296-012-2424-2

    authors: Işik M,Çinar E,Cemal Kizilarslanoğlu M,Özbek E,Etgül S,Kiraz S

    更新日期:2013-08-01 00:00:00

  • Osteoporosis in chronic liver disease: a case-control study.

    abstract::Osteoporosis has become an increasingly recognized complication among patients with chronic liver disease (CLD). The aim of the present study was to assess the prevalence and risk factors of osteoporosis in patients with CLD (primary biliary cirrhosis and chronic viral hepatitis B or C patients) in comparison with a g...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-009-1071-8

    authors: Wariaghli G,Mounach A,Achemlal L,Benbaghdadi I,Aouragh A,Bezza A,El Maghraoui A

    更新日期:2010-05-01 00:00:00

  • The effect of different treatment time of millimeter wave on chondrocyte apoptosis, caspase-3, caspase-8, and MMP-13 expression in rabbit surgically induced model of knee osteoarthritis.

    abstract::The aim of this study is to observe the effect of different treatment time of millimeter wave (MMW) on chondrocyte apoptosis, caspase-3, caspase-8, and matrix metalloproteinase-13 (MMP-13) in rabbit knee osteoarthritis induced by anterior cruciate ligament transection (ACLT). Thirty-two New Zealand White rabbits were ...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-011-2080-y

    authors: Xia L,Luo QL,Lin HD,Zhang JL,Guo H,He CQ

    更新日期:2012-09-01 00:00:00

  • Outcome of Boyd-McLeod procedure for recalcitrant lateral epicondylitis of elbow.

    abstract::Various surgical procedures including percutaneous and open release and arthroscopic procedures have been described to treat recalcitrant tennis elbow. We present the outcome of Boyd-McLeod surgical procedure for tennis elbow resistant to non-operative treatment in twenty-seven patients (twenty-nine limbs). Boyd McLeo...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-010-1450-1

    authors: Reddy VR,Satheesan KS,Bayliss N

    更新日期:2011-08-01 00:00:00

  • A portable dermatoscope for easy, rapid examination of periungual nailfold capillary changes in patients with systemic sclerosis.

    abstract::Microvascular lesions are a predominant feature in systemic sclerosis (SSc) and seem to play a central pathogenic role. The presence of nailfold capillary abnormalities is useful in diagnosing SSc. Capillaroscopy, however, usually requires special equipment and may be time consuming. Dermatoscope has been presented as...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-010-1532-0

    authors: Muroi E,Hara T,Yanaba K,Ogawa F,Yoshizaki A,Takenaka M,Shimizu K,Sato S

    更新日期:2011-12-01 00:00:00

  • Monitoring of therapeutic efficacy in a patient with RS₃PE syndrome by serologic variables and radiographic methods.

    abstract::We describe a typical case of a patient with remitting seronegative symmetrical synovitis and pitting edema (RS(3)PE) syndrome. He underwent a successful clinical course monitored by serologic variables and radiographic methods. Serum levels of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), matrix me...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-009-1142-x

    authors: Kawashiri SY,Nakano M,Kawakami A,Eguchi K

    更新日期:2010-11-01 00:00:00

  • Leflunomide: friend or foe for systemic lupus erythematosus?

    abstract::Leflunomide is a new immunosuppressive medicine that has been effectively used in the therapy of rheumatoid arthritis and subsequently used with success in animal models and patients with systemic lupus erythematosus (SLE). However, its use has also been associated with significant and serious adverse reactions involv...

    journal_title:Rheumatology international

    pub_type: 杂志文章,评审

    doi:10.1007/s00296-012-2508-z

    authors: Wu GC,Xu XD,Huang Q,Wu H

    更新日期:2013-02-01 00:00:00

  • Markers of endothelial damage and repair in Takayasu arteritis: are they associated with disease activity?

    abstract::The current clinical and laboratory parameters of Takayasu arteritis (TA) are insufficient for proper assessment of disease activity. The aims of this study were to investigate the markers of endothelial injury and repair, including circulating endothelial cell (CEC), circulating endothelial progenitor cell (CEPC) and...

    journal_title:Rheumatology international

    pub_type: 杂志文章

    doi:10.1007/s00296-013-2937-3

    authors: Dogan S,Piskin O,Solmaz D,Akar S,Gulcu A,Yuksel F,Cakır V,Sari I,Akkoc N,Onen F

    更新日期:2014-08-01 00:00:00