Histochemical staining. Its use for detection of glyceraldehyde-3-phosphate dehydrogenase in skeletal muscle.

Abstract:

:Histochemical staining of glyceraldehyde-3-phosphate dehydrogenase activity in fresh-frozen muscle tissue may be performed using a beta-nicotinamide adenine dinucleotide (beta-NAD) reduced-dehydrogenase tetrazolium-reductase linked reaction. The phosphate-buffered incubating medium contains glyceraldehyde-3-phosphate, beta-NAD, nitro blue tetrazolium, and edetic acid. This technique produces a fine reticular pattern of blue staining in muscle fibers. In human muscle specimens, type I fibers stain darker than type II fibers.

journal_name

Arch Pathol Lab Med

authors

Brumback RA,Susag ME,Gerst JW

subject

Has Abstract

pub_date

1981-08-01 00:00:00

pages

419-20

issue

8

eissn

0003-9985

issn

1543-2165

journal_volume

105

pub_type

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