Abstract:
:The hippocampal slice is a popular model system in which to study the cellular properties of long-term potentiation (LTP). Synaptogenesis induced by exposure to ice-cold artificial cerebrospinal fluid (ACSF), however, raises the concern that morphological correlates of LTP might be obscured, especially in mature slices. Here we demonstrate that preparation of mature hippocampal slices at room temperature (approximately 25 degrees C) maintains excellent ultrastructure and a synapse density comparable to perfusion-fixed hippocampus. These results suggest that slices prepared at room temperature might provide a better basis from which to detect LTP-related changes in synapse number and morphology.
journal_name
Neuropharmacologyjournal_title
Neuropharmacologyauthors
Bourne JN,Kirov SA,Sorra KE,Harris KMdoi
10.1016/j.neuropharm.2006.06.020subject
Has Abstractpub_date
2007-01-01 00:00:00pages
55-9issue
1eissn
0028-3908issn
1873-7064pii
S0028-3908(06)00201-2journal_volume
52pub_type
杂志文章abstract::An earlier finding that three systems were responsible for the uptake of gamma-aminobutyric acid by synaptosomes was corroborated. In addition, the existence of three uptake systems for RS-nipecotic acid and beta-alanine was demonstrated. We have called these systems high affinity, medium affinity and low affinity upt...
journal_title:Neuropharmacology
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doi:10.1016/0028-3908(86)90183-8
更新日期:1986-05-01 00:00:00
abstract::A genetic component is associated with the development of many forms of epilepsy. Recently, mutations in the GABAA receptor have been linked to several inherited epilepsies. One of these mutations is a non-conservative change of alanine to aspartate in the third transmembrane domain of the alpha1 subunit. To determine...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2003.11.015
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journal_title:Neuropharmacology
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(85)90090-5
更新日期:1985-01-01 00:00:00
abstract::A cDNA encoding the human metabotropic glutamate receptor type 4 (hmGluR4) was isolated from human brain cDNA libraries by cross-hybridization with rat mGluR4 probes. The deduced amino acid sequence of human mGluR4 consists of 912 residues and shows a sequence identity of 96% to the amino acid sequence of rat mGluR4. ...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(94)00149-m
更新日期:1995-02-01 00:00:00
abstract::The effects of intracerebroventricular (i.c.v.) administration of NT on the activity of midbrain DA neurons were studied in rats using single unit recording techniques. NT (i.c.v., 50 micrograms) was found to have no significant effect on the spontaneous activity of DA cells. On the other hand, NT treatment significan...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(90)90119-c
更新日期:1990-11-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
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更新日期:2016-03-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2007.08.010
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journal_title:Neuropharmacology
pub_type: 杂志文章,评审
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更新日期:2017-08-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章,评审
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更新日期:2017-09-15 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(93)90020-4
更新日期:1993-11-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/s0028-3908(03)00041-8
更新日期:2003-04-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(83)90026-6
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2013.04.056
更新日期:2013-10-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.neuropharm.2014.09.022
更新日期:2015-09-01 00:00:00
abstract::Changes in the levels of mRNA for the NR1 subunit of the glutamate NMDA receptor and in NMDA-sensitive glutamate binding were investigated in consecutive sections of the prefrontal cortex and striatum of control and Parkinson's disease (PD) post-mortem brain using in-situ hybridisation and receptor autoradiography. Bo...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/s0028-3908(98)00219-6
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2016.03.031
更新日期:2016-08-01 00:00:00
abstract::The obesity epidemic is one of the biggest health challenges globally and rates have tripled since 1975. Overeating is the largest determinant of obesity, yet little is understood of the neural mechanisms underlying why individuals consume food regardless of satiety. The lateral hypothalamic (LH) input to the ventral ...
journal_title:Neuropharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.neuropharm.2019.05.014
更新日期:2019-08-01 00:00:00
abstract::Hormones may produce long-term effects on excitability by regulating K+ channel gene expression. Previous studies demonstrated that administration of dexamethasone, a glucocorticoid receptor agonist, to adrenalectomized rats, rapidly induces Kv1.5 K+ channel expression in the ventricle of the hear. Here, RNase protect...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Central sensitization represents a key mechanism mediating chronic pain, a major clinical problem lacking effective treatment options. LIM-domain kinases (LIMKs) selectively regulate several substrates, e.g. cofilin and cAMP response element-binding protein (CREB), that profoundly affect neural activities, such as syn...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2017.06.031
更新日期:2017-10-01 00:00:00
abstract::Clinically effective antidepressants are thought to exert their therapeutic effects by facilitating central monoamine neurotransmission. However, recent data showing that neurokinin-1 receptor (NK1R) antagonists have antidepressant properties in both animal and clinical studies raise the possibility that classical ant...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2004.02.013
更新日期:2004-06-01 00:00:00
abstract::Anti-emetic drugs such as the tachykinin NK(1) receptor antagonists are useful to control emesis induced by diverse challenges. Evidence suggests pungent capsaicin-like TRPV1 activators also have broad inhibitory anti-emetic activity. However, pungent compounds are associated with undesirable effects including adverse...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2009.10.002
更新日期:2010-02-01 00:00:00
abstract::Ethylcholine mustard aziridinium ion (AF64A), or vehicle, was infused bilaterally (3 nmol/3 microliter per side) into the lateral ventricles of rats. The effect of such treatment on various cholinergic responses was measured in the hippocampus, cortex and striatum. Potassium-stimulated release of acetylcholine from su...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(85)90031-0
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(83)90223-x
更新日期:1983-12-01 00:00:00
abstract::NMDA receptors (NMDAr) are widely expressed throughout the brain on many cell types, and loss of function of these receptors (ie: NMDAr hypofunction) is a candidate mechanism explaining working memory impairment in schizophrenia. However, the cellular source driving the working memory deficits caused by NMDAr hypofunc...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2020.108103
更新日期:2020-07-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/0028-3908(91)90203-n
更新日期:1991-02-01 00:00:00
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journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2013.03.027
更新日期:2013-08-01 00:00:00
abstract::24S-hydroxycholesterol (24S-HC) is a brain-derived product of lipid metabolism present in the systemic circulation, where its level can change significantly in response to physiological and pathophysiological conditions. Here, using electrophysiological and optical approaches, we have found a high sensitivity to 24S-H...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2017.01.030
更新日期:2017-05-01 00:00:00
abstract::Excitatory neurotransmission mediated by N-methyl-d-aspartate receptors (NMDARs) is fundamental to learning and memory and, when impaired, causes certain neurological disorders. NMDARs are heterotetrameric complexes composed of two GluN1 [NR1] and two GluN2(A-D) [NR2(A-D)] subunits. The GluN2 subunit is responsible fo...
journal_title:Neuropharmacology
pub_type: 杂志文章
doi:10.1016/j.neuropharm.2011.12.018
更新日期:2012-04-01 00:00:00