Abstract:
:A quantitative bioassay for nerve growth factor (NGF) has been previously described which employs PC13 rat pheochromocytoma cells. The basis of this assay is that rapid neurite regeneration by PC12 cells pretreated ('primed') with NGF for at least a week is quantitatively related to the amount of NGF present in the culture medium. The present experiments demonstrate that NGF-pretreated PC12 cells retain their priming for over a year when appropriately frozen and stored and that such frozen cells can be employed for the quantitative bioassay of NGF. The major advantage of this assay is that large numbers of cells can be primed at a time (in suspension culture) and stored in aliquots until future use. The newly described bioassay employs the frozen primed cells essentially as reagents and avoids the need to have freshly primed cultures on hand.
journal_name
Brain Resjournal_title
Brain researchauthors
Rukenstein A,Greene LAdoi
10.1016/0006-8993(83)91218-0subject
Has Abstractpub_date
1983-03-14 00:00:00pages
177-80issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(83)91218-0journal_volume
263pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Effect of a mu-opioid agonist (D-Ala2,N-MePhe4,Gly5-ol-enkephalin, DAGO), on glycine (Gly)-induced chloride current (IGly) was investigated in the periaqueductal gray (PAG) neurons acutely dissociated 1-2-week-old Wistar rats by the use of nystatin-perforated patch recording configuration under voltage-clamp condition...
journal_title:Brain research
pub_type: 杂志文章
doi:
更新日期:1996-09-23 00:00:00
abstract::We investigated the effect of losartan, a nonpeptide angiotensin II (Ang II)-type 1 (AT1) receptor antagonist, on the responses evoked by Ang II and L-glutamate (L-Glu) in the rostral ventrolateral medulla (RVLM). Adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were anesthetized with halothane ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91344-7
更新日期:1994-12-05 00:00:00
abstract::Experimental allergic encephalomyelitis (EAE) was induced in young male Lewis rats. Blood-brain barrier permeability to radiotracers of different molecular sizes was studied at intervals after induction using a tissue sampling technique. The results were correlated to the clinical picture and to the histological appea...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90249-x
更新日期:1984-06-08 00:00:00
abstract::The projections of serotonin-containing neurons of the midbrain raphe nuclei (nucleus raphe dorsalis, nucleus centralis superior) are studied by analysis of axonal transport of labeled amino acids. These results are correlated with regional alterations of serotonin content following midbrain raphe lesions which produc...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90144-x
更新日期:1976-05-14 00:00:00
abstract::The avian pineal exhibits a daily rhythm in the synthesis and secretion of the hormone, melatonin, which is involved in maintaining temporal order within the circadian system of some species. The pineal is richly innervated by sympathetic nerves which originate in the superior cervical ganglia (SCG) and, in the chicke...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90636-n
更新日期:1992-01-13 00:00:00
abstract::The effects of intracerebral injections of brain-gut peptides in the preoptic region on the secretion of luteinizing hormone (LH) and prolactin (PRL) were examined in ovariectomized, estrogen-primed rats. Gastrin or cholecystokinin-octapeptide injection in the preoptic region induced a moderate increase of circadian r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90330-1
更新日期:1987-05-05 00:00:00
abstract::A differential role has been suggested for two important areas in the neural circuitry of stress, central nucleus of the amygdala (CeA) and bed nucleus of stria terminalis (BNST) in the extended amygdala, in regulating fear versus anxiety. Since chronic stress enhances anxiety and consolidation of aversive memories, w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)04162-8
更新日期:2003-03-07 00:00:00
abstract::Antisense oligodeoxynucleotides (ODNs) are used for the selective inhibition of gene expression. Antisense ODNs are promising tools for the investigation of physiological implications of proteins in the central nervous system of rodents in vivo. We have previously demonstrated that a phosphorothioate antisense ODN to ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00463-0
更新日期:1997-08-08 00:00:00
abstract::The carotid body of the mouse is ideally suited for in vitro photometric and fluorometric studies using transillumination. It is small, thin and translucent. Photometric recordings were made by using a dual wavelength method. Wavelengths of 550 nm (maximal absorbance, MA) and 540 nm (isosbestic point, IP) were employe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90019-8
更新日期:1985-03-18 00:00:00
abstract::Mild NMDA receptor activation is correlated with neuroprotection in models of cerebral ischemia. Neuroprotection with NMDA manifests as a form of ischemic tolerance and involves the induction of cellular stress systems sensitive to disturbances in cellular calcium homeostasis. Unilateral micro-injection of 10, 160 and...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.10.014
更新日期:2009-01-09 00:00:00
abstract::High-intensity stimulation of the supraorbital region elicits, together with a blink reflex, a reflex inhibition of the jaw-closing muscles in normal man. The response differs from the well known inhibition obtained by intra- and perioral stimulation in two main features. Firstly, it consists of a single late silent p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90366-5
更新日期:1986-04-23 00:00:00
abstract::Cell death was due to activation of the receptor responsive to N-methyl-D-aspartate (NMDA). Even during a 30-min incubation, glutamate toxicity was unaffected by 5 microM CNQX and was greater than 90% blocked by 160 nM MK-801, a specific antagonist of the NMDA receptor. The extent of toxicity was dependent on the age ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91244-u
更新日期:1991-09-27 00:00:00
abstract::In anaesthetized cats, effects of iontophoretic application of angiotensin II were studied in the nuclei of the tractus solitarius. Specifically, its effects were tested on single respiratory neurones, having rhythmic activity related temporally with the phrenic nerve discharge, and on presumed reflex interneurones, w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91232-7
更新日期:1987-03-24 00:00:00
abstract::Hemicholinium-3 (HCh-3) is a potent and specific inhibitor of the high-affinity choline transport process (HAChT) localized on cholinergic neurons. In this study, the specific binding of [3H]HCh-3 (120 Ci/mmol) was characterized in crude synaptic membranes prepared from rat brain. The binding of [3H]HCh-3 to forebrain...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90451-2
更新日期:1985-12-02 00:00:00
abstract::In Parkinson disease (PD), the subthalamic nucleus (STN) becomes hyperactive (disinhibited), which is reported to cause excitotoxic damage to midbrain dopaminergic neurons. Here, we examined whether silencing of the hyperactive STN by chronic bilateral deep brain stimulation (DBS) increased the survival of midbrain do...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.08.082
更新日期:2006-11-20 00:00:00
abstract::We investigated the effects of neonatal optic nerve transection on cortical acetylcholinesterase (AChE) activity in hooded rats during postnatal development and following behavioral manipulation after weaning. AChE reaction product was quantified on digitized images of histochemically stained sections in layer IV of p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.12.080
更新日期:2007-03-30 00:00:00
abstract::In an attempt to demonstrate a protective effect against convulsions induced by hyperbaric oxygen (OHP), aminooxyacetic acid (AOAA at 2.5 mg/kg) was administered subcutaneously to 60 white leghorn cockerels (aged 21-28 days) at periods of 1-72 h before exposure to OHP. A transient protection was noted 1-8 h after admi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90753-9
更新日期:1983-10-16 00:00:00
abstract::ATP has been recognized as an important extracellular signaling molecule and P2X receptors are membrane ion channels activated by the binding of extracellular ATP. Since both AQP4 and P2X7 receptor (P2X7R) are known to be present in astrocytes, we examined whether P2X7R activation plays a role in the regulation of AQP...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.12.023
更新日期:2008-02-21 00:00:00
abstract::Recently, a novel method for detection of DNA synthesis has been developed based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction). In the present stud...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.092
更新日期:2010-03-10 00:00:00
abstract::Damage to the cerebral endothelium from ischemia could exacerbate brain injury by altering vascular integrity, but little is known concerning the response of cerebral endothelial cells to hypoxia. To address this issue, cerebral capillary endothelial cells were isolated from 14-day-old rats, grown to confluence, and p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90367-0
更新日期:1994-08-08 00:00:00
abstract::The role of GABA in NMDA-dependent long term depression (LTD) in the medial vestibular nuclei (MVN) was studied on rat brainstem slices. High frequency stimulation (HFS) of the primary vestibular afferents induces a long lasting reduction of the polysynaptic (N2) component of the field potentials recorded in the dorsa...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00895-w
更新日期:1995-11-20 00:00:00
abstract::The distribution of creatine kinase BB-isoenzyme in the human central nervous system (CNS) was investigated immunohistochemically and the findings were compared with the distributions of tubulin and astroprotein. Creatine kinase BB-isoenzyme existed both in neurons and astrocytes, and was universally distributed withi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)91338-0
更新日期:1983-04-11 00:00:00
abstract::We have previously demonstrated that ibotenate (IBO)-stimulated polyphosphoinositide (PPI) hydrolysis is increased for a long period in the amygdala/pyriform cortex (AM/PC) of amygdala (AM)- and hippocampal (HIPP)-kindled rats. This finding indicates that enhanced function of the PPI-coupled excitatory amino acid (EAA...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90370-o
更新日期:1992-01-08 00:00:00
abstract::This study investigated the role of strain and vendor differences on the outcome of focal cerebral ischemia in the rat induced by occlusion of the middle cerebral artery by an intraluminal thread. The cortical infarct volumes (Mean +/- S.E.M.) were: 14.2 +/- 6.2 mm3 in Simonsen Laboratories Sprague-Dawley rats; 84.0 +...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00033-m
更新日期:1995-03-27 00:00:00
abstract::Neonatal rats were exposed to lead from parturition to weaning via the milk of dams drinking 0.2% lead acetate solution. The alterations of long-term potentiation (LTP) and paired-pulse facilitation (PPF) of hippocampal dentate gyrus in adult rats (90-115 days) following developmental lead exposure were studied in viv...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00739-2
更新日期:1998-09-28 00:00:00
abstract::Previous research has shown that, in freely moving laboratory rats, the odors of benzyl alcohol, camphor, carvacrol, isopentenyl methyl sulfide, 2-propylthietane, salicylaldehyde, trimethylthiazoline, and xylene (plus other compounds) elicit high amplitude bursts of roughly 20-Hz waves (beta-waves) in the olfactory bu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03225-5
更新日期:2002-01-11 00:00:00
abstract::Afferents from the nucleus reticularis thalami (RT) to the thalamic ventrobasal complex were studied in the rat by looking for degenerating terminals after selective neurotoxic lesion of RT using injections of kainic acid. Several lines of evidence are presented indicating that RT afferents terminate in the VB by F ty...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90607-8
更新日期:1983-07-04 00:00:00
abstract::Electrical stimulation of the dorsal hippocampal formation of the rat was employed to determine the effect of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D, on induced seizure thresholds. Stereotaxic injection of 100 micrograms or 50 micrograms 1,25-dihydroxyvitamin D3 in 2 microliter propyle...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91153-3
更新日期:1984-04-23 00:00:00
abstract::The effect of hippocampal kindling on neuronal and glial marker proteins was studied in the rat by immunochemical methods. In hippocampus, pyriform cortex and amygdala there was an increase in glial fibrillary acidic protein (GFAP), indicating reactive gliosis, and an increase in the glycolytic enzyme NSE, suggesting ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90791-9
更新日期:1990-10-29 00:00:00
abstract::The regulation of spike generation in rat midbrain dopamine (DA) neurons was investigated using in vitro intracellular recordings. DA neurons fired long (greater than 1.8 ms) action potentials that exhibited comparatively depolarized spike thresholds (approx. -35 to -45 mV). Depolarization of the DA neuron increased t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90488-w
更新日期:1990-07-30 00:00:00