Early intravenous infusion of sodium nitrite protects brain against in vivo ischemia-reperfusion injury.

Abstract:

BACKGROUND AND PURPOSE:The rate of nitric oxide (NO) generation from nitrite is linearly dependent on reductions in oxygen and pH levels. Recently, nitrite-derived NO has been reported to exert a profound protection against liver and heart ischemia-reperfusion injury. In this study, we hypothesized that nitrite would be reduced to NO in the ischemic brain and exert NO-dependent neuroprotective effects. METHODS:Cerebral ischemia-reperfusion injury was induced by intraluminal thread occlusion of middle cerebral artery in the adult male rats. Solutions of sodium nitrite were infused intravenously at the time of reperfusion. Sodium nitrate and carboxy-PTIO (30 minutes before ischemic surgery), a direct NO scavenger, were infused for comparisons. RESULTS:Nitrite reduced infarction volume and enhanced local cerebral blood flow and functional recovery. The effects were observed at concentrations of 48 nmol and 480 nmol, but not at 4800 nmol nitrite and 480 nmol nitrate. The neuroprotective effects of nitrite were inhibited completely by the carboxy-PTIO. The 480 nmol nitrite attenuated dihydroethidium activity, 3-nitrotyrosine formation, and lipid peroxidation in the ischemic brain. CONCLUSIONS:Nitrite exerted profound neuroprotective effects with antioxidant properties in the ischemic brains. These results suggest that nitrite, as a biological storage reserve of NO, may be a novel therapeutic agent in the setting of acute stroke.

journal_name

Stroke

journal_title

Stroke

authors

Jung KH,Chu K,Ko SY,Lee ST,Sinn DI,Park DK,Kim JM,Song EC,Kim M,Roh JK

doi

10.1161/01.STR.0000245116.40163.1c

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

2744-50

issue

11

eissn

0039-2499

issn

1524-4628

pii

01.STR.0000245116.40163.1c

journal_volume

37

pub_type

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