Rapid, sensitive detection of myoglobulinemia by improved counterimmunoelectrophoresis in cases of acute myocardial infarction.

Abstract:

:A counterimmunoelectrophoresis technique for detection of serum myoglobin (Mb) was improved using non-ionic polymer dextran. Precipitin lines were graded according to their strength, which was ascertained by radioimmunoassay data. By this method, serum Mb in concentrations of 500 ng./ml. before stain and of 200 ng./ml. after stain were detected. Electrophoretic time was 60 minutes. Among 32 cases of acute myocardial infarction (AMI) whose blood samples were collected within 24 hours after disease onset, precipitin lines were detected in 25 cases (78%) before stain and 31 cases (97%) after stain. Considering the early peak concentration time (approximately 10 hours) of serum Mb after AMI onset, diagnosis becomes more rapid and exact with this method, especially in severe cases.

journal_name

Am Heart J

journal_title

American heart journal

authors

Hiramori K,Sumiyoshi T,Motegi S,Honda T,Kimata S,Hirosawa K,Kawai H,Kondo A,Iwaasa M,Miyoshi K

doi

10.1016/0002-8703(78)90084-4

subject

Has Abstract

pub_date

1978-08-01 00:00:00

pages

187-90

issue

2

eissn

0002-8703

issn

1097-6744

pii

0002-8703(78)90084-4

journal_volume

96

pub_type

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