Gossypol inhibition of adenylate cyclase.

abstract：:The effect of fatty acid binding proteins (FABPs) on two key steps of microsomal phosphatidic acid formation was examined. Rat liver microsomes were purified by size-exclusion chromatography to remove endogenous cytosolic fatty acid and fatty acyl-CoA binding proteins while recombinant FABPs were used to avoid cross-c...

journal_title：Archives of biochemistry and biophysics

authors： Jolly CA,Hubbell T,Behnke WD,Schroeder F

更新日期：1997-05-01 00:00:00

abstract：:Prior exposure of guinea pig macrophages to LPS (lipopolysaccharide) resulted in reduced cAMP-generating responses to prostaglandin E1 and epinephrine. LPS-induced refractoriness was diminished when LPS treatment was carried out in the presence of an inhibitor of prostaglandin synthesis, hydrocortisone, or indomethaci...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Mori Y,Ui M

更新日期：1986-05-01 00:00:00

abstract：:The cytochrome P-450-dependent cholesterol side chain cleavage system of the brain has been studied using nonsynaptic mitochondria as the source of enzymatic activity. The system has been found to bind cholesterol and 11-deoxycorticosterone, producing type I difference spectra, whereas the binding of pregnenolone indu...

journal_title：Archives of biochemistry and biophysics

authors： Walther B,Ghersi-Egea JF,Minn A,Siest G

更新日期：1987-05-01 00:00:00

abstract：:Antioxidative flavonoids, ubiquitously included in vegetables, fruits and teas, are expected to prevent degenerative diseases. It is unclear, however, whether flavonoids can enter the cellular nuclei and suppress the oxidative damage of DNA. Here, several flavonoids at the physiological concentration of 10 microM were...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa K,Uehara M,Yanagitani H,Hashimoto T

更新日期：2006-11-15 00:00:00

abstract：:The ferric and ferrous forms of bovine erythrocyte green hemeprotein react with hydroperoxides to form higher oxidation state intermediates with absorbance maxima in the Soret region at 426 and 422 nm, respectively. In the absence of an appropriate reductant, these intermediates undergo rapid bleaching reactions. 2,2'...

journal_title：Archives of biochemistry and biophysics

authors： Xu F,DeFilippi LJ,Ballou DP,Hultquist DE

更新日期：1993-02-15 00:00:00

abstract：:Five functional isoforms of a novel plant thiol methyltransferase from the leaves of cabbage (Brassica oleracea L.) were purified to electrophoretic homogeneity. Pooled, partly purified preparations of the enzyme were previously shown to methylate thiol compounds released upon the hydrolysis of glucosinolates. The enz...

journal_title：Archives of biochemistry and biophysics

authors： Attieh J,Sparace SA,Saini HS

更新日期：2000-08-15 00:00:00

abstract：:The nuclear Ah receptor from mouse hepatoma (Hepa-1c1c9) cells is a 176-kDa multimeric protein which is stable under conditions of up to 1 M KCl. Under denaturing conditions, the Hepa-1 nuclear receptor can be dissociated into a ligand-binding subunit of Mr approximately 91,000. The identity of subunits that compose t...

journal_title：Archives of biochemistry and biophysics

authors： Prokipcak RD,Denison MS,Okey AB

更新日期：1990-12-01 00:00:00

abstract：:The cardiac contraction-relaxation cycle is controlled by a sophisticated set of machinery. Of particular interest, is the revelation that allosteric networks transmit effects of binding at one site to influence troponin complex dynamics and structural-mediated signaling in often distal, functional sites in the myofil...

journal_title：Archives of biochemistry and biophysics

authors： Marques MA,Parvatiyar MS,Yang W,de Oliveira GAP,Pinto JR

更新日期：2019-03-15 00:00:00

abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

journal_title：Archives of biochemistry and biophysics

authors： Matsuda T,Iwata H

更新日期：1988-06-01 00:00:00

abstract：:Complete structures are described for three urinary oligodextrins from one patient with type II and one patient with type III glycogen storage disease. GLC-MS, direct probe MS, and 1H NMR demonstrate two heptasaccharides and one hexasaccharide containing only alpha 1-4 and alpha 1-6 linkages. The observation that all ...

journal_title：Archives of biochemistry and biophysics

authors： Kumlien J,Grönberg G,Nilsson B,Månsson O,Zopf D,Lundblad A

更新日期：1989-03-01 00:00:00

abstract：:Orotate phosphoribosyltranferase (OPRTase) catalyzes the formation of orotidine 5'-monophosphate from the nitrogenous base orotate and alpha-D-5-phosphoribosyl-1-pyrophosphate (PRPP). While it is known that Mg2+ is necessary for catalysis, the mechanism of activation of the phosphoribosyl transfer by Mg2+ remains uncl...

journal_title：Archives of biochemistry and biophysics

authors： Bhatia MB,Grubmeyer C

更新日期：1993-06-01 00:00:00

abstract：:The glutamylation of methotrexate has been evaluated in H35 hepatoma cells in vitro as a function of the conditions of culture. Glutamylation yields methotrexate polyglutamate with two to five additional glutamate residues and is a saturable process. The rate of glutamylation increases little above 10 microM extracell...

journal_title：Archives of biochemistry and biophysics

authors： Nimec Z,Galivan J

更新日期：1983-10-15 00:00:00

abstract：:Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

journal_title：Archives of biochemistry and biophysics

authors： Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

更新日期：2011-05-15 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates biological and toxicological effects by binding to its agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Previously we demonstrated that flavonoids suppressed the TCDD-induced DNA-binding activity of the AhR in a st...

journal_title：Archives of biochemistry and biophysics

authors： Mukai R,Shirai Y,Saito N,Fukuda I,Nishiumi S,Yoshida K,Ashida H

更新日期：2010-09-01 00:00:00

abstract：:Binding sites having the properties of high-affinity receptors for activated alpha 2-macroglobulin (alpha 2M) have been purified over 100-fold from membranes of spontaneously transformed NIH-3T3 cells (J. A. Hanover, S.-y. Cheng, M. C. Willingham, and I. H. Pastan [1983] J. Biol. Chem. 258, 370-377). To identify the m...

journal_title：Archives of biochemistry and biophysics

authors： Hanover JA,Rudick JE,Willingham MC,Pastan I

更新日期：1983-12-01 00:00:00

abstract：:The ionization of 4-nitroimidazole to 4-nitroimidazolate was investigated as a function of ionic strength. The apparent pKa varies from 8.99 to 9.50 between 0.001 and 1.0 M ionic strength, respectively, at 25 degrees C. The ionic strength dependence of this ionization is anomalous. The binding of 4-nitroimidazole by h...

journal_title：Archives of biochemistry and biophysics

authors： Taylor KC,Vitello LB,Erman JE

更新日期：2000-10-15 00:00:00

abstract：:The presence of multiple proteases in the culture filtrate of Streptomyces moderatus was detected. After preliminary purification by ammonium sulfate precipitation and decolorization using DEAE-cellulose, the fractionation of various proteases was carried out using CM-trisacryl cation-exchange chromatography. By this ...

journal_title：Archives of biochemistry and biophysics

authors： Chandrasekaran S,Dhar SC

更新日期：1987-09-01 00:00:00

abstract：:Prostaglandin endoperoxide H synthases-1 and -2 (PGHS-1 and -2) are integral membrane proteins associated with the luminal surface of the endoplasmic reticulum (ER) and the nuclear envelope (NE). The C-terminal sequences of PGHSs end in -Ser/Pro-Thr-Glu-Leu (-S/PTEL), a sequence similar to one of the known ER targetin...

journal_title：Archives of biochemistry and biophysics

authors： Song I,Smith WL

更新日期：1996-10-01 00:00:00

abstract：:Dystrophic chicken breast muscle mitochondria contain significantly less mitochondrial creatine kinase than normal breast muscle mitochondria. Breast muscle mitochondria from normal 16- to 40-day-old chickens contain approximately 80 units of mitochondrial creatine kinase per unit of succinate:INT (p-iodonitrotetrazol...

journal_title：Archives of biochemistry and biophysics

authors： Bennett VD,Hall N,DeLuca M,Suelter CH

更新日期：1985-07-01 00:00:00

abstract：:Transcription factor Nkx2.5, essential for heart development, regulates cardiomyocyte-specific gene expression through combinatorial interactions with other cardiac-restricted (GATA4 and dHAND) or ubiquitous (p300) transcription regulators. Here we demonstrate that Nkx2.5 and p53 synergistically activate the promoter ...

journal_title：Archives of biochemistry and biophysics

authors： Kojic S,Nestorovic A,Rakicevic L,Protic O,Jasnic-Savovic J,Faulkner G,Radojkovic D

更新日期：2015-03-01 00:00:00

abstract：:The rate constant for quenching, kq, of the phosphorescence of 6-bromo-2-naphthyl sulfate (BNS) by cytochromes c, cytochrome c peroxidase, catalase, and myoglobin has been measured as a function of temperature and solvent viscosity. In aqueous solution at pH 7.0 for cytochromes c and myoglobin the value of kq is nearl...

journal_title：Archives of biochemistry and biophysics

authors： Beckham S,Cook MP,Karki L,Luchsinger MM,Whitlock VR,Wu Y,Zhang Q,Schuh MD

更新日期：1994-05-01 00:00:00

abstract：:The Metabotropic glutamate receptor 2 (mGluR2) is involved in several neurological and psychiatric disorders and is an attractive drug target. It is believed to form a strict dimer and the dimeric assembly is necessary for glutamate induced activation. Although many studies have focused on glutamate induced conformati...

journal_title：Archives of biochemistry and biophysics

authors： Singh DR,Pandey K,Mishra AK,Pandey P,Vivcharuk V

更新日期：2021-01-15 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:Adenylate cyclase activation by GTP and octopamine as well as basal activity (in the presence of Mg2+) have been studied as a function of membrane structure in plasma membranes from brain of the dipterous Ceratitis capitata. Benzyl alcohol and lidocaine, but not phenobarbital, inhibited the three activities to the sam...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Gavilanes FG,Municio AM

更新日期：1991-05-01 00:00:00

abstract：:The cDNA probe H-9, originally constructed to recognize a portion of the mRNA for lapine synovial collagenase, also hybridized with a RNA of the same size (approximately 2.0 kb) isolated from activated lapine articular chondrocytes. Primary, monolayer cultures of lapine articular chondrocytes did not contain detectabl...

journal_title：Archives of biochemistry and biophysics

authors： Lin CW,Phillips SL,Brinckerhoff CE,Georgescu HI,Bandara G,Evans CH

更新日期：1988-07-01 00:00:00

abstract：:The importance of the sn-glycerol- 3-phosphate (G-3-P) electron transfer shuttle in hormonal regulation of gluconeogenesis was examined in hepatocytes from rats with decreased mitochondrial G-3-P dehydrogenase activity (thyroidectomized) or increased G-3-P dehydrogenase activity [triiodothyronine (T(3)) or dehydroepia...

journal_title：Archives of biochemistry and biophysics

authors： Kneer N,Lardy H

更新日期：2000-03-01 00:00:00

abstract：:Reports suggest that microRNAs have implications in the development of several diseases including cancer. It is therefore believed that miRs may act as therapeutic targets for cancer treatment. The treatment of ovarian cancer is mainly obstructed by lack of biomarkers and efficient drug targets. Against this backdrop,...

journal_title：Archives of biochemistry and biophysics

authors： Si L,Jia Y,Lin R,Jian W,Yu Q,Yang S

更新日期：2019-06-15 00:00:00

abstract：:The physiological role of the peroxisomal fatty acyl-CoA beta-oxidizing system (FAOS) is not yet established. We speculated that there might be a relationship between peroxisomal degradation of long-chain fatty acids in the liver and the biosynthesis of bile acids. This was investigated using [1-14C]butyric acid and [...

journal_title：Archives of biochemistry and biophysics

authors： Hayashi H,Miwa A

更新日期：1989-11-01 00:00:00

abstract：:Primary chondrocyte cell cultures and explants of bovine articular cartilage were subjected to cyclic hydrostatic pressure in a novel computer-controlled pressure chamber designed for this purpose. The cultures were labeled with 5 microCi/ml 35SO4 and simultaneously pressurized with 5 MPa load for 1.5 or 20 h with pre...

journal_title：Archives of biochemistry and biophysics

authors： Parkkinen JJ,Ikonen J,Lammi MJ,Laakkonen J,Tammi M,Helminen HJ

更新日期：1993-01-01 00:00:00

abstract：:The interaction of sarcoplasmic reticulum Ca(2+)-ATPase with the Mg.ATP analogues Rh(H2O)4ATP and Co(NH3)4ATP have been examined. Co(NH3)4ATP slowly inactivates Ca(2+)-ATPase in a first order process, with a rate constant of 1.13 x 10(-3) s-1 and an apparent inactivation constant, KI, of 32 mM. Rh(H2O)4ATP likewise in...

journal_title：Archives of biochemistry and biophysics

authors： Kuntzweiler TA,Grisham CM

更新日期：1992-05-15 00:00:00