Abstract：
:Sulfatases hydrolyze sulfate esters on a variety of molecules including glycosaminoglycans, sulfoglycolipids, and cytosolic steroids. These enzymes are found in a wide range of organisms with their basic enzymatic mechanisms broadly conserved. In mammals, many of the sulfatases localize in the lysosome and exhibit enzymatic activity on a small aryl substrate such as 4-methylumbelliferyl sulfate (4-MUS). They are known as arylsulfatases. Sulf-1 and Sulf-2 have been cloned and identified as sulfatases that release sulfate groups on the C-6 position of GlcNAc residue from an internal subdomain in intact heparin. Hence, these enzymes are endosulfatases. The Sulfs are secreted in an active form into conditioned medium of transfected Chinese hamster ovary (CHO) cells. In this chapter, arylsulfatase and endoglucosamine-6-sulfatase assays for the Sulfs are described. A solid-phase binding assay is also detailed, which allows investigation of the ability of the Sulfs to modulate the interaction of heparin-binding proteins with immobilized heparin. The example illustrated is vascular endothelial growth factor (VEGF). This assay is projected to be very useful in the investigation of the biological functions of the Sulfs.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Uchimura K,Morimoto-Tomita M,Rosen SDdoi
10.1016/S0076-6879(06)16015-2subject
Has Abstractpub_date
2006-01-01 00:00:00pages
243-53eissn
0076-6879issn
1557-7988pii
S0076-6879(06)16015-2journal_volume
416pub_type
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更新日期：2009-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/B978-0-12-385118-5.00022-0
更新日期：2011-01-01 00:00:00
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journal_title：Methods in enzymology
pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
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更新日期：2014-01-01 00:00:00
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journal_title：Methods in enzymology
pub_type： 杂志文章
doi：10.1016/bs.mie.2018.03.001
更新日期：2018-01-01 00:00:00
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journal_title：Methods in enzymology
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journal_title：Methods in enzymology
pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
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journal_title：Methods in enzymology
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Methods in enzymology
pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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