Abstract:
:Anaplasma marginale and A. phagocytophylum are intracellular rickettsiae that cause bovine anaplasmosis and human granulocytic anaplasmosis, respectively. The ultimate vaccine for the control of anaplasmosis would be one that reduces infection and transmission of the pathogen by ticks. Effective vaccines for control of anaplasmosis are not available despite attempts using different approaches, such as attenuated strains, infected erythrocyte and tick cell-derived purified antigens, and recombinant pathogen and tick-derived proteins. Three lines of functional analyses were conducted by our laboratory to characterize host-tick-Anaplasma interactions to discover potential vaccine candidate antigens to control tick infestations and the infection and transmission of Anaplasma spp.: (1) characterization of A. marginale adhesins involved in infection and transmission of the pathogen, (2) global expression analysis of genes differentially expressed in HL-60 human promyelocytic cells in response to infection with A. phagocytophilum, and (3) identification and characterization of tick-protective antigens by expression library immunization (ELI) and analysis of expressed sequence tags (EST) in a mouse model of tick infestations and by RNA interference in ticks. These experiments have resulted in the characterization of the A. marginale MSP1a as an adhesin for bovine erythrocytes and tick cells, providing support for its use as candidate vaccine antigen for the control of bovine . Microarray analysis of genes differentially expressed in human cells infected with A. phagocytophilum identified key molecules involved in pathogen infection and multiplication. The screening for tick-protective antigens resulted in vaccine candidates reducing tick infestation, molting, and oviposition and affecting Anaplasma infection levels in ticks.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
de la Fuente J,Ayoubi P,Blouin EF,Almazán C,Naranjo V,Kocan KMdoi
10.1196/annals.1374.081subject
Has Abstractpub_date
2006-10-01 00:00:00pages
416-23eissn
0077-8923issn
1749-6632pii
1078/1/416journal_volume
1078pub_type
杂志文章abstract::The dynamics and kinematics of saccades in a patient with severe ocular myasthenia were studied before and after treatment with intravenous immunoglobulin (IVIG). Before therapy, horizontal saccades were hypometric, but faster than similar-sized saccades made by normal subjects. During a 5-minute test period, saccades...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2008.03712.x
更新日期:2009-05-01 00:00:00
abstract::A range of imidazoline derivatives are known to be effective stimulators of insulin secretion, and this response correlates with closure of ATP-sensitive potassium channels in the pancreatic beta-cell. However, mounting evidence indicates that potassium channel blockade may form only part of the mechanism by which imi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1999.tb09364.x
更新日期:1999-06-21 00:00:00
abstract::These initial studies show that deregulated expression of ALG7 affects diverse cellular functions crucial to development, including proliferation, differentiation, and morphogenesis. Furthermore, the data suggest multiple genetic targets for ALG7 and provide the basis for future dissection of these developmentally rel...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1998.tb09648.x
更新日期:1998-04-15 00:00:00
abstract::The dramatic changes in overall mode of life experienced since 1989 by the people in Central and Eastern Europe (CEE) provides a good opportunity to study the aging processes and the status of the elderly. In the 1980s, CEE countries were already at an advanced stage of demographic aging of their populations. The firs...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1396.041
更新日期:2007-10-01 00:00:00
abstract::We cloned a kappa 3-related opioid receptor, and although it is still not clear whether this clone corresponds to the kappa 3 receptor itself or is a related gene product, the extensive antisense mapping and the antibody immunoreactivity strongly associate this clone with the kappa 3 receptor. Our approach also indica...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1995.tb17491.x
更新日期:1995-05-10 00:00:00
abstract::Proopiomelanocortin (POMC) and proenkephalin (PE) are synthesized at the endoplasmic reticulum and transported to the trans-Golgi network (TGN) where they are sorted and packaged into dense-core granules of the regulated secretory pathway (RSP). The mechanism of sorting POMC and PE to the RSP in neuroendocrine cells w...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2002.tb04504.x
更新日期:2002-10-01 00:00:00
abstract::Physicians have used various forms of antipyretic therapy since antiquity to lower the temperature of febrile patients. Nevertheless, it has yet to be determined whether the benefits of antipyretic therapy outweigh its risks. It is not known, for example, if core temperatures encountered during the febrile state ever ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1998.tb08328.x
更新日期:1998-09-29 00:00:00
abstract::During the past decade, a considerable body of evidence has emerged showing that circumstances during the fetal period may have lifelong programming effects on different body functions with a considerable impact on disease susceptibility. The purpose of this article is to provide a synopsis of these findings and their...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1367.026
更新日期:2006-11-01 00:00:00
abstract::The identification of sensitive assay formats capable of distinguishing islet autoreactive T cells directly ex vivo in blood is a major goal in type 1 diabetes research. Whereas much effort has been made to identify suitable assay formats, relatively little attention has been paid to optimizing the quality of cell pre...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1337.005
更新日期:2004-12-01 00:00:00
abstract::Aberrations in estrogen signaling increase breast cancer risk. Molecular mechanisms that impact breast cancer initiation, promotion, and progression can be investigated using genetically engineered mouse models. Increasing estrogen receptor alpha (ERα) expression levels twofold is sufficient to initiate and promote br...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2011.06086.x
更新日期:2011-07-01 00:00:00
abstract::We studied the effects of change of head position in the pitch plane on the ability to hold horizontal eccentric gaze in five patients with cerebellar disorders. All patient showed a change in the time constant of horizontal centripetal drift when the head was pitched forward or back. This result suggests that otolith...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1325.075
更新日期:2005-04-01 00:00:00
abstract::Human speech perception seems to involve the ability to recognize groupings of speech sounds rather than component phonemes, and to distinguish between permuted orders of items within sequences as holistic entities. Humans can use this Holistic Pattern Recognition (HPR) not only with speech and music, but also with se...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1976.tb25532.x
更新日期:1976-01-01 00:00:00
abstract::This study measured on-road driving behavior in subjects with bilateral vestibular loss (BVL). Data included point-of-regard (what the driver is looking at and attending to), gaze stability (the performance of the vestibulo-ocular reflex), and head movement, during complex maneuvers such as changing lanes, cornering, ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2008.03733.x
更新日期:2009-05-01 00:00:00
abstract::Invasive plants cause substantial economic and environmental damage throughout the world. However, eradication of most invasive species is impossible and, in some cases, undesirable. An alternative is to slow the spread of an invasive species, which can delay impacts or reduce their extent. We identify three main area...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2011.06313.x
更新日期:2012-02-01 00:00:00
abstract::Hybrid liver systems are being developed as temporary extracorporeal liver support therapy. The overview given here emphasizes the development of both hepatocyte culture models for bioreactors and of systems for clinical therapy. In vitro studies demonstrate long term external metabolic function in isolated primary he...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2001.tb03843.x
更新日期:2001-11-01 00:00:00
abstract::As illustrated in Figure 1, a disturbance of the intracellular Ca2+ homeostasis is thought to be a common pathogenic factor for the generation of secondary nerve cell damage that develops after brain trauma or stroke or during the course of neurodegenerative diseases. A neuronal Ca2+ overload which may result from an ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1997.tb48409.x
更新日期:1997-10-15 00:00:00
abstract::Substantial evidence suggests that stress can alter the general toxicological properties of the substituted amphetamines (AMPs) as well as their psychostimulant properties. Research concerning the interactions between stress and the neurotoxicity associated with the AMPs is, however, limited. Our previous work demonst...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1996.tb17438.x
更新日期:1996-10-31 00:00:00
abstract::Our previous studies have demonstrated that the methanol extract of heat-processed Panax ginseng C.A. Meyer exerts antioxidative, antiinflammatory, and anti-tumor-promoting effects. In the present study, we examined the antiinflammatory effects of several ginsenosides (Rb(1), Rc, Re, Rg(1), Rg(3)) derived from P. gins...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2002.tb04672.x
更新日期:2002-11-01 00:00:00
abstract::The development of glucocorticoid (GC) resistance is a serious problem that complicates the treatment of immune-related diseases, such as asthma, ulcerative colitis, and hematologic cancers. hGRalpha and hGRbeta are two isoforms of the human glucocorticoid receptor, which differ in the structural composition of the ca...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1351.001
更新日期:2006-06-01 00:00:00
abstract::Mouse pheochromocytoma cells (MPCs) provide an excellent model system for investigating the effects of hypoxia on catecholamine enzyme genes and on transcription factors mediating stress responses. RT-PCR detects rapid, transient increases in PNMT mRNA in hypoxic MPC 712 cells. Additionally, elevation of mRNAs encodin...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2002.tb04434.x
更新日期:2002-10-01 00:00:00
abstract::Estrogen synthesized in situ plays a more important role in breast cancer cell proliferation than does circulating estrogen. Aromatase is the enzyme that converts androgen to estrogen and is expressed at a higher level in breast cancer tissue than in surrounding noncancer tissue. A promising route of chemoprevention a...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2002.tb04116.x
更新日期:2002-06-01 00:00:00
abstract::In epithelia and endothelia, overall resistance (TER) is determined by all ion-conductive structures, such as membrane channels, tight junctions, and the intercellular space, whereas the epithelial capacitance is due to the hydrophobic phase of the plasma membrane. Impedance means alternating current resistance and, i...
journal_title:Annals of the New York Academy of Sciences
pub_type: 历史文章,杂志文章,评审
doi:10.1111/j.1749-6632.2012.06540.x
更新日期:2012-06-01 00:00:00
abstract::The human ABO(H) blood group phenotypes arise from the evolutionarily oldest genetic system found in primate populations. While the blood group antigen A is considered the ancestral primordial structure, under the selective pressure of life-threatening diseases blood group O(H) came to dominate as the most frequently ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/nyas.13694
更新日期:2018-08-01 00:00:00
abstract::Explanations of the socioeconomic gradient in health status must account for the observations that the gradient cuts across a wide range of disease processes and is capable of replicating itself on new disease processes as they emerge in society. Understanding this pattern requires an understanding of how human organi...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.1999.tb08107.x
更新日期:1999-01-01 00:00:00
abstract::With the progressive aging of the world's population, immunosenescence is rapidly becoming a clinical concern as it accounts for a higher incidence of severe infections and poor response to vaccines. To identify nutritional approaches that may counteract immunosenescence is of obvious importance in clinical practice. ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1749-6632.2009.05264.x
更新日期:2010-03-01 00:00:00
abstract::The melanocortin (MC)-4 receptor participates in regulating body weight homeostasis. We demonstrated early that acute blockage of the MC-4 receptor increases food intake and relieves anorexic conditions in rats. Our recent studies show that 4-week chronic blockage of the MC-4 receptor leads to robust increases in food...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2003.tb03164.x
更新日期:2003-06-01 00:00:00
abstract::Previous studies, which included predominantly Caucasian populations, examined psychiatric and physical illness and associated suicide risk. We used a nationwide database to reassess the findings in an Asian population, and also analyzed the influence of different onset timing of psychiatric and physical illness and t...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/nyas.14216
更新日期:2020-02-01 00:00:00
abstract::The biological consequences of chronic consumption of Maillard reaction products (MRPs) on renal function in health and renal disease are still incompletely understood. We investigated the metabolic and renal effects of a diet with varying MRP content in healthy and subtotally nephrectomized rats. Male Wistar rats wer...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1196/annals.1333.055
更新日期:2005-06-01 00:00:00
abstract::Despite advances in technology and medicine, death itself remains an immutable certainty. Indeed, the acceptance and understanding of our mortality are among the enduring metaphysical challenges that have confronted human beings from the beginning of time. How have we sought to cope with the inevitability of our morta...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/nyas.12474
更新日期:2014-11-01 00:00:00
abstract::Tocopherols are known to undergo metabolism to phytyl chain-shortened metabolites excreted in urine. We sought to characterize the pathway, including associated enzymes, involved in this biotransformation. We previously found that human hepatoblastoma (HepG2) cultures metabolized tocopherols to their corresponding sho...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1331.002
更新日期:2004-12-01 00:00:00